Thesis (M.S.)--University of Wisconsin--Madison, 1973. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph.D.)--Indiana University, Dept. of History, 2006. / "Title from dissertation home page (viewed July 12, 2007)." Source: Dissertation Abstracts International, Volume: 67-10, Section: A, page: 3939. Adviser: John H. Hanson.

Thesis (Th. M.)--Grace Theological Seminary, 1983. / Abstract. Bibliography: leaves 88-93.

Thesis (Ph. D.)--Ohio State University, 1974. / Includes vita. Includes bibliographical references (leaves 281-286). Available online via OhioLINK's ETD Center.

Thesis ( (M.Sc.)(Wildlife Management)--University of Pretoria, 1999. / Summary in Afrikaans and English. Includes bibliographical references.

The primary structure of a 38 kDa protein isolated from membrane preparations of African trypanosomes (Trypanosoma brucei rhodesiense) was determined by protein and DNA sequencing. Searching of the protein database with the trypanosome translated amino acid sequence identified glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) from various prokaryotic and eukaryotic organisms as the optimal scoring protein. Surprisingly, the eukaryotic trypanosome enzyme showed the highest degree of sequence identity with the corresponding enzyme from the prokaryote Escherichia coli. Using recombinant DNA techniques, the trypanosome molecule was expressed in Escherichia coli and found to be enzymatically active, thus confirming the identity of the molecule as an NAD+-dependent glycerol 3-phosphate dehydrogenase. A monoclonal antibody specific for the 38 kDa protein was used to localize the enzyme to glycosomes. The enzyme has a pi of 9.0, a net charge of +9 at physiological pH and contains the peroxisome-like targeting tripeptide SKM at its C-terminus, all characteristic of glycosomal enzymes. Amino acids predicted to be involved in the NAD+-dependent glycerol 3-phosphate dehydrogenase active site have diverged from those of the mammalian enzyme. Kinetic analyses of the trypanosome GPD and GPD from rabbit muscle showed that the Km values of the two enzymes are different The data suggests that the trypanosome protein may be a candidate target for rational drug design. Northern and Southern blot analyses showed that the trypanosome NAD+-dependent glycerol 3- phosphate dehydrogenase was translated from a single transcript and that only two gene copies exist thus making this molecule an attractive target for knockout mutagenesis. A second molecule, an abundant 11 kDa membrane protein, was also purified from African trypanosomes. This protein cross-reacted with monoclonal antibodies originally generated against the lipophosphoglycan-associated protein of Leishmania donovani. Immunoblot analysis showed that the 11 kDa molecule was present in a variety of species of kinetoplastids. It was found in several species and subspecies of African trypanosomes and was present in low amounts in bloodstream forms and in larger amounts in procyclic, epimastigote and metacyclic life cycle stages. The molecule was present in procyclic trypanosome membranes at approximately [special characters omitted] molecules per cell. Its wide distribution in kinetoplastids and its membrane disposition suggested a name for this class of molecules (kinetoplastid membrane protein-11) and for the molecule characterized in this thesis (trypanosome kinetoplastid membrane protein-11). The kinetoplastid membrane protein-11 molecule was purified from Trypanosoma brucei rhodesiense by organic solvent extraction and octyl-Sepharose chromatography and a 14 amino acid internal peptide sequence was obtained by gas phase microsequencing. This sequence matched a translated Leishmania donovani kinetoplastid membrane protein-11 sequence, thus suggesting the use of the Leishmania sequence as a probe to select for the Trypanosoma gene. Screening of a trypanosome cosmid library with the Leishmania probe, in combination with a series of polymerase chain reaction amplifications from both genomic DNA and cDNA, allowed the determination of the entire DNA sequence and corresponding translated amino acid sequence of the trypanosome kinetoplastid membrane protein-11. The 92 amino acid sequence showed 18 percent sequence divergence from the corresponding molecule of the related kinetoplastid Leishmania donovani donovani^ including one key amino acid at position 45 which may be of functional relevance. The secondary structure of the trypanosome molecule was predicted to form two amphipathic helices connected by a random-coil segment, and suggests that it would interact with lipid bilayers in the parasite cell membrane. Northern and Southern blot analyses using the T.b. rhodesiense ViTat 1.1 clone showed that the trypanosome molecule was translated from a single transcript and that there was only a single gene copy, thus making this molecule an attractive target for knockout mutagenesis. / Graduate

<p> Ma th&egrave;se s&rsquo;appuie sur des recherches existantes sur le f&eacute;minisme dans les litt&eacute;ratures africaines pour retracer le rapport entre les modes de repr&eacute;sentations du corps f&eacute;minin et ses implications aussi bien sociales que politiques. Allant au-del&agrave; d&rsquo;une simple repr&eacute;sentation du corps f&eacute;minin dans la litt&eacute;rature produite en fran&ccedil;ais par des &eacute;crivains africains (hommes et femmes), je soutiens que le corps de la femme est devenu l&rsquo;un des lieux de (r&eacute;sistance au) pouvoir &agrave; la fois pendant la colonisation et apr&egrave;s l&rsquo;ind&eacute;pendance de l&rsquo;Afrique francophone. Dans ma th&egrave;se, j&rsquo;utilise des critiques d&rsquo;Odile Cazenave, Pierre Bourdieu, Gayatri Spivak et Julia Kristeva pour montrer que le corps de la femme en Afrique est litt&eacute;ralement devenu une m&eacute;taphore de la nation enti&egrave;re. En fin de compte, ma th&egrave;se analyse la repr&eacute;sentation du corps f&eacute;minin noir dans son usage, ses fonctions, son espace et ses relations avec les pouvoirs sociaux et politiques. Je discute aussi des conditions mentales et structurales qui ont rendu possible les m&eacute;canismes qui ont facilite la construction d&rsquo;un imaginaire du corps f&eacute;minin noir. En m&rsquo;appuyant sur les p&eacute;r&eacute;grinations de la femme africaine plus connue sous le nom de Venus Hottentot, je soutiens en outre que les personnages f&eacute;minins noirs sont en perp&eacute;tuel combat pour r&eacute;clamer leur corps,ses usages, comme la manifestation ultime de leur droit d&rsquo;exister.</p><p>

Forms of Affiliation maps new literary geographies that cut across national, postcolonial, local, and global frameworks. Focusing on fiction from the 1950s to the present-day from South Africa, Malawi, Zambia, and Zimbabwe, it demonstrates how writers from these nations have developed new genres of fiction in popular media to imagine changing modes of interconnection across space. Popular media—including newspapers, magazines, and their digital iterations—are vital literary outlets in southern Africa and often the only means for underrepresented populations to find a voice in public discourse. Crucially, many of the genres in these publications do not fit neatly into European literary categories. They also envision Africanness and blackness within a variety of overlapping spatial scales, from the township to the diaspora, thus challenging the common conception of southern African literatures as tied primarily to nationalist projects. Through the analysis and translation of hundreds of stories from publications such as African Parade, Africa!, the Malawi News, and the Chimurenga Chronic, I identify four generic categories of southern African fiction: “migrant forms,” “township tales,” “newspaper short stories,” and “literary time-machines.” Across its chapters, Forms of Affiliation shows how these genres make visible combinations of form, meaning, and geography that are obscured by traditional literary categories. / African and African American Studies

This dissertation explores the significance of the youth in the popular politics of 20th-century Ghana. Based on two and half years of archival and field research in Ghana and Britain, the dissertation investigates the political agency of the youth, especially in the domains of youth associations, student politics, and popular culture. It also examines the structural factors in the colonial and postcolonial periods that shaped youth political engagement, and how youth worked within and without these structural frames to shape popular politics. I argue that youth-centered politics has been a motive force in Ghanaian popular politics. It opened up space for subalterns to be important players in colonial politics especially as catalysts of anti-colonial nationalism. In the post-colonial period, youth politics, mostly in the form of university students’ political activism, articulated public interests and was a bulwark against the authoritarianism of civilian and military governments. The dissertation charts the changing manifestations of Ghanaian youth political identity and formation from the early 1900s, when Britain completed its formal imposition of colonial rule on the territory that is present-day Ghana, to the political crisis of the late 1970s in which students and youth played crucial roles. The dissertation is a corrective to elite-focused accounts of political developments in Ghana’s history. It establishes youths as historically significant players who have shaped the country’s political ideas, values and practices. The dissertation also contributes to the renewed and growing focus on intergenerational relations, generational identity, and youth in scholarship on Africa. / History

This study re-examines historical change in western Africa during the eighteenth and nineteenth centuries through the lens of cotton textiles; that is by focusing on the production, exchange and consumption of cotton cloth, including the evolution of clothing practices, through which the region interacted with other parts of the world. It advances a recent scholarly emphasis to re-assert the centrality of African societies to the history of the early modern trade diasporas that shaped developments around the Atlantic Ocean. However, this study argues that Atlantic exchanges in western Africa relied significantly upon Indian Ocean commercial networks as well. By analyzing a wide range of European travel accounts, commercial records and correspondence, visual images, maps, and oral narratives, this study breaks with well-rehearsed Atlantic conceptual frameworks for this period of African history to instead emphasize the global historical context in which Africans made decisions that impacted their communities and the larger world. The geographic focus of this study covers a large part of western Africa: from the Sahara desert in the north, northeast to the Niger bend, southeast to coastal Sierra Leone and west to the Senegal river valley and Atlantic coast. Its main findings emphasize the diversity of western African engagements with global commerce via textile production and consumption across time and space. / African and African American Studies