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Desenvolvimento de ensaios para a determinação da atividade enzimática das aldeído desidrogenases de cordados invertebrados. / Assays developments for the determination of the enzymatic activity of aldehydes dehydrogenases of invertebrate chordates.Amaral, Fábio Neves do 04 April 2018 (has links)
O presente projeto representa uma extensão dos paradigmas que criamos com estudos de simulação molecular para compreender as frequentes mudanças de estrutura e função das Aldeído Desidrogenases (ALDHs) durante a evolução. As ALDHs formam uma superfamília de proteínas que catalisam a oxidação de vários aldeídos, mas as origens evolutivas das preferências pelos seus substratos são pouco conhecidas. Apesar de possuírem uma elevada identidade sequencial, duas destas ALDHs, a ALDH1 e a ALDH2, exibem distintos papéis funcionais de sinalização celular e detoxicação, respectivamente. Através de prévia análise computacional e logenética, identicamos que, curiosamente, as ALDH1s de organismos invertebrados, Branchiostoma oridae e Ciona intestinalis apresentam características estruturais mais semelhantes às de suas ALDH2s do que das ALDH1 típicas. Isto sugere que essas ALDH1s divergentes podem ter evoluído na direção da atividade de degradação de aldeídos pequenos e tóxicos, que parecem representar a função ancestral das ALDH2s eucarióticas. Nossa análise identicou três assinaturas de aminoácidos localizadas na área interna do canal de entrada do substrato (CES) que distinguem as ALDH1 das ALDH2. Desta forma, constatamos que as ALDH1s possuem um CES amplo e desobstruído, consistente com o fato destas enzimas catalisarem aldeídos de cadeia longa como o retinaldeído, que é um precursor de vias de sinalização por retinóides. Em contraste, as ALDH2s possuem o CES pouco volumoso e constrito, consistente com sua função na degradação de pequenos aldeídos tóxicos e reativos, como o acetaldeído. Neste projeto o nosso objetivo é analisar a correlação funcional e estrutural entre as ALDH1 e ALDH2 presentes em B. oridae e C. intestinalis para desvendar e compreender seus papéis funcionais e evolutivos em cordados. Especicamente, testaremos a hipótese de que as três assinaturas descritas constituem o núcleo fundamental da preferência por substratos, e que sua inversão por mutações sitio-dirigidas entre ALDH1 e ALDH2 modicará a preferência de substrato de acordo com a origem da assinatura. Se conrmado experimentalmente, este será um exemplo pioneiro de reversão evolutiva molecular, que terá impacto direto sobre as interpretações atuais sobre controversa lei de Dollo da irreversibilidade evolutiva. / This project represents an extension of the paradigms that we created from our molecular simulation studies to understand the frequent structure and function changes of aldehyde dehydrogenases (ALDH) during evolution. The ALDHs form a superfamily of proteins that catalyze the oxidation of several aldehydes, but the evolutionary origins of their substrate preference are unknown. Despite having a high sequence identity, two of these ALDHs, the ADLH1, and ALDH2, exhibit distinct functional roles of cellular signaling and detoxication, respectively. Through previous computational and phylogenetic analysis, we found that, interestingly, the ALDH1s of invertebrate organisms (Branchiostoma oridaeand Ciona intestinalis) show structural features more similar to their ALDH2s than the typical ALDH1. It suggests that these divergent ALDH1sevolved to provide small aldehydes detoxication pattern, what seems to represent the ancestral eukaryotic ALDH2s function. Our analysis also identied three aminoacidsignatures, located internally in the substrate entry channel (SEC), that distinguishes the ALDH1 from ALDH2. Thus, we nd that ALDH1s have a wide, open and unobstructed SEC, consistent with the fact that these enzymes catalyze bulky long-chainaldehydes, like retinaldehyde, a precursor of important signaling pathways. In contrast, the ALDH2s have a small and constricted SEC, consistent with the degradation function of small aldehydes, like the toxic metabolite acetaldehyde. In this project, our objective is to understand the functional and structural correlation between ALDH1 and ALDH2 found in B. oridaeand C. intestinalisto discover and comprehend their functional and evolutionary roles in chordates. Specically, we will test the hypothesis that the three signatures described above are the fundamental core of the substrate preference, based on the signature origin. If conrmed experimentally, this will be a pioneeringexample of evolutionary molecular reversion, impacting directly on the current interpretations of the controversial Dollos Law of Irreversibility.
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Engineering of an enzyme cocktail for biodegradation of petroleum hydrocarbons based on known enzymatic pathways and metagenomic techniquesBaburam, Cindy 07 1900 (has links)
Ph. D. (Department of Biotechnology, Faculty of Applied and Computer Sciences), Vaal University of Technology. / Hydrocarbon pollution is becoming a growing environmental concern in South Africa and globally. This inadvertently supports the need to identify enzymes for their targeted degradation. The search for novel biocatalysts such as monooxygenases, alcohol dehydrogenases and aldehyde dehydrogenases, have relied on conventional culture-based techniques but this allows sourcing of the biomolecules from only 1-10 % of the microbial population leaving the majority of the biomolecules unaccounted for in 90-99 % of the microbial community. The implementation of a metagenomics approach, a culture-independent technique, ensures that more or less than 100 % of the microbial community is assessed. This increases the chance of finding novel enzymes with superior physico-chemical and catalytic traits. Hydrocarbon polluted soils present a rich environment with an adapted microbial diversity. It was thus extrapolated that it could be a potential source of novel monooxygenases, alcohol dehydrogenases (ADH) and aldehyde dehydrogenases (ALDH) involved in hydrocarbon degradation pathways. Therefore, the aim of the study was to extract metagenomic DNA from hydrocarbon contaminated soils and construct a metagenomic fosmid library and screen the library for monooxygenases, alcohol dehydrogenases (ADH) and aldehyde dehydrogenases (ALDH). Accordingly, the fosmid library was constructed from metagenome of hydrocarbon-contaminated soil. Then the library was functionally screened using hexadecane, octadecene and cyclohexane as substrates and fifteen positive clones were selected. The fosmid constructs of the positive clones were sequenced using PacBio next generation sequencing platform. The sequences were de novo assembled and analysed using CLC Genomic Workbench. The open reading frames (ORF) of the contigs were identified by blasting the contigs against uniport database. Accordingly, four novel genes namely amo-vut1, aol-vut3, dhy-sc-vut5 and dhy-g-vut7 that showed close similarity with our target enzymes were further analysed in silico and codon-optimized as per Escherichia coli codon preference. The codon adjusted sequences were synthesised and cloned into pET30a(+) expression vector. However, it is worth noting that expression of amo-vut1 was not successful since it was later identified to be a multi-pass member protein, which made it insoluble despite the use of detergent to the effect.
There is a need to meticulously genetically engineer amo-vut1 to remove the signal and other membrane-bound peptides while maintaining its activity. Yet the other three constructs were successfully transformed and expressed in E. coli BL21 (DE3). The enzymes were purified and characterized and cocktail for hydrolysis of hexanol was succesfully engineered based on AOL-VUT3, DHY-SC-VUT5 and DHY-G-VUT7. Therefore, novel enzymes were mined from metagenome of fossil-oil contaminated soil and effective hydrocarbon-degrading enzyme cocktails containing their combination were successfully engineered.
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Analyse cinétique des rétinaldéhydes déshydrogénases recombinantes de type 3 et 4 de sourisSima, Aurélia 08 1900 (has links)
Les Rétinal déshydrogénases (RALDHs) catalysent irréversiblement la déshydrogénation du Rétinal en Acide Rétinoïque (AR) qui est impliqué dans l’embryogenèse et la différenciation tissulaire. Pour comprendre le rôle dans la biosynthèse de l’AR des RALDHs type 3 et 4 de souris, nous avons déterminé leurs propriétés cinétiques ainsi que leur comportement en présence de différents inhibiteurs.
Les tests enzymatiques sont effectués avec une préparation d’enzyme recombinante, tagguée avec 6 histidines, purifiée sur colonne Ni-NTA (Qiagen). L’activité enzymatique est évaluée en quantifiant la production d’AR par chromatographie liquide à haute performance (HPLC) en phase inversée. Les constantes cinétiques ont été déterminées pour les isomères du rétinal tout-trans, 9-cis et 13-cis.
La RALDH4 catalyse les isomères 9-cis et 13-cis de rétinal, elle présente un faible KM (3μM) pour les deux isomères et a une efficacité catalytique élevée pour le 9-cis rétinal 3.4 fois supérieure au 13-cis rétinal. La RALDH3 est spécifique au tout-trans rétinal avec un KM de 4 μM et une efficacité élevée.
β-Ionone, inhibiteur possible pour la RALDH4, inhibe l’activité avec le rétinal 9-cis et 13-cis, mais n’influence pas l’activité de la RALDH3. Le para-hydroxymercuribenzoïque (p-HMB) inhibe l’activité de deux isoenzymes. Le cation MgCl2 augmente par 3 fois l’oxydation du rétinal 13-cis par la RALDH4, diminue l’oxydation du 9-cis rétinal et influence faiblement la RALDH3.
Ces données enrichissent les connaissances sur les caractéristiques cinétiques des RALDHs recombinantes de souris de types 3 et 4 et fournissent des éclaircissements sur la biogenèse de l’acide rétinoïque in vivo. / SUMMARY
Retinal dehydrogenases (RALDHs) catalyze the dehydrogenation of retinal into retinoic acids (RA) that are required for embryogenesis and tissue differentiation. This study sought to determine the detailed kinetic properties of 2 mouse RALDHs, namely RALDH3 and 4, for retinal isomer substrates, to better define their specificities in RA isomer synthesis.
RALDH3 and 4 were expressed as His-tagged proteins and affinity-purified. RALDH3 oxidized all-trans retinal with high catalytic efficiency but did not show activity for either 9-cis or 13-cis retinal substrates. RALDH4 was inactive for all-trans retinal substrate, exhibited high activity for 9-cis retinal oxidation, and oxidized 13-cis retinal with lower catalytic efficiency.
β-ionone, a potent inhibitor of RALDH4 activity, suppressed 9-cis and 13-cis retinal oxidation competitively, but had no effect on RALDH3 activity. The p-HMB inhibited the activity for both RALDH3 and RALDH4. The divalent cation MgCl2 activated 13-cis retinal oxidation by RALDH4 by 3-fold, slightly decreased 9-cis retinal oxidation, and did not significantly influence RALDH3 activity.
These data extend the kinetic characterization of RALDH3 and 4, providing their specificities for retinal isomer substrates, which should help in determining their functions in the synthesis of RAs in specific tissues.
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Analyse cinétique des rétinaldéhydes déshydrogénases recombinantes de type 3 et 4 de sourisSima, Aurelia 08 1900 (has links)
Les Rétinal déshydrogénases (RALDHs) catalysent irréversiblement la déshydrogénation du Rétinal en Acide Rétinoïque (AR) qui est impliqué dans l’embryogenèse et la différenciation tissulaire. Pour comprendre le rôle dans la biosynthèse de l’AR des RALDHs type 3 et 4 de souris, nous avons déterminé leurs propriétés cinétiques ainsi que leur comportement en présence de différents inhibiteurs.
Les tests enzymatiques sont effectués avec une préparation d’enzyme recombinante, tagguée avec 6 histidines, purifiée sur colonne Ni-NTA (Qiagen). L’activité enzymatique est évaluée en quantifiant la production d’AR par chromatographie liquide à haute performance (HPLC) en phase inversée. Les constantes cinétiques ont été déterminées pour les isomères du rétinal tout-trans, 9-cis et 13-cis.
La RALDH4 catalyse les isomères 9-cis et 13-cis de rétinal, elle présente un faible KM (3μM) pour les deux isomères et a une efficacité catalytique élevée pour le 9-cis rétinal 3.4 fois supérieure au 13-cis rétinal. La RALDH3 est spécifique au tout-trans rétinal avec un KM de 4 μM et une efficacité élevée.
β-Ionone, inhibiteur possible pour la RALDH4, inhibe l’activité avec le rétinal 9-cis et 13-cis, mais n’influence pas l’activité de la RALDH3. Le para-hydroxymercuribenzoïque (p-HMB) inhibe l’activité de deux isoenzymes. Le cation MgCl2 augmente par 3 fois l’oxydation du rétinal 13-cis par la RALDH4, diminue l’oxydation du 9-cis rétinal et influence faiblement la RALDH3.
Ces données enrichissent les connaissances sur les caractéristiques cinétiques des RALDHs recombinantes de souris de types 3 et 4 et fournissent des éclaircissements sur la biogenèse de l’acide rétinoïque in vivo. / SUMMARY
Retinal dehydrogenases (RALDHs) catalyze the dehydrogenation of retinal into retinoic acids (RA) that are required for embryogenesis and tissue differentiation. This study sought to determine the detailed kinetic properties of 2 mouse RALDHs, namely RALDH3 and 4, for retinal isomer substrates, to better define their specificities in RA isomer synthesis.
RALDH3 and 4 were expressed as His-tagged proteins and affinity-purified. RALDH3 oxidized all-trans retinal with high catalytic efficiency but did not show activity for either 9-cis or 13-cis retinal substrates. RALDH4 was inactive for all-trans retinal substrate, exhibited high activity for 9-cis retinal oxidation, and oxidized 13-cis retinal with lower catalytic efficiency.
β-ionone, a potent inhibitor of RALDH4 activity, suppressed 9-cis and 13-cis retinal oxidation competitively, but had no effect on RALDH3 activity. The p-HMB inhibited the activity for both RALDH3 and RALDH4. The divalent cation MgCl2 activated 13-cis retinal oxidation by RALDH4 by 3-fold, slightly decreased 9-cis retinal oxidation, and did not significantly influence RALDH3 activity.
These data extend the kinetic characterization of RALDH3 and 4, providing their specificities for retinal isomer substrates, which should help in determining their functions in the synthesis of RAs in specific tissues.
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