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Studies on food-derived antihypertensive peptidesYan, Tsz-king, Eric. January 2000 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references.
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Evidence supporting a dual glucocorticoid and mineralocorticoid role for the elasmobranch steroid 1[alpha]-hydroxycorticosteroneEvans, Andrew Neil, 1979- 10 September 2012 (has links)
In mammals distinct steroid hormones termed mineralocorticoids (MCs) and glucocorticoids (GCs) regulate hydromineral balance and the stress response, respectively. In contrast, it is thought that a single corticosteroid, 1[alpha]-hydroxycorticosterone (1[alpha]-B) serves as both a GC and MC in elasmobranchs. I investigated the putative dual MC and GC roles of 1[alpha]-B by examining ex vivo regulation of interrenal 1[alpha]-B synthesis by osmoregulatory and stress hormones in the euryhaline stingray Dasyatis sabina. A commercial enzyme-linked immunoassay was adapted for the quantification of 1[alpha]-B. I also isolated cDNA sequences encoding two rate-limiting steroidogenic enzymes, the steroidogenic acute regulatory protein (StAR) and P450 cholesterol side-chain cleavage (P450scc), and characterized the steroidogenic activity of the encoded proteins using a heterologous expression system. Both the stress hormone adrenocorticotropic hormone (ACTH) and the antinatriuretic peptide angiotensin II (ANG II) were potently steroidogenic in ex vivo interrenal cultures, whereas C-type natriuretic peptide (CNP) inhibited 1[alpha]-B synthesis. StAR and P450scc mRNA levels were increased by 24 h incubation with ACTH and decreased by both ANG II and CNP. To examine changes in osmoregulatory hormone systems that impinge upon 1[alpha]-B synthesis, I also isolated the cDNA sequences of the ANG II and CNP receptors, AT and NPR-B. Both AT and NPR-B mRNA levels were significantly elevated in osmoregulatory tissues of freshwater (FW; Lake Monroe, FL) versus saltwater (SW; Corpus Christi Bay, TX) populations of D. sabina. Interrenal StAR and NPR-B mRNA levels were also significantly higher in FW individuals. The physiological roles of 1[alpha]-B were further investigated in vivo by examining the effects of stress and FW transfer on interrenal synthesis of 1[alpha]-B. Plasma 1[alpha]-B and glucose were significantly elevated by hook-and-line capture stress, indicating that 1[alpha]-B acts in classical GC fashion to facilitate the stress response. In contrast, 1[alpha]-B was significantly decreased 24 h after SW-FW transfer. In light of the osmotic strategy of euryhaline elasmobranchs, this result is consistent with a MC role for 1[alpha]-B. Taken together, the results of this research strongly support a dual role for 1[alpha]-B in facilitating both hydromineral balance and the stress response in elasmobranchs. / text
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The functional interaction of mouse secretin and angiotensin II receptorsWong, Ka-yan, Karen, 黃嘉欣 January 2010 (has links)
published_or_final_version / Biological Sciences / Master / Master of Philosophy
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Mechanisms of Amiodarone and Desethylamiodarone Cytotoxicity in Human Lung CellsBLACK, JEANNE 26 November 2009 (has links)
Amiodarone (AM) is a potent antidysrhythmic agent which can cause potentially life-threatening pulmonary fibrosis, and N-desethylamiodarone (DEA) is a metabolite of AM that may contribute to the toxicity of AM in vivo. Recent evidence has implicated the involvement of the renin-angiotensin system (RAS) in the initiation and progression of amiodarone-induced pulmonary toxicity.
In cultured HPL1A human peripheral lung epithelial cells, we found AM to be converted to DEA minimally (< 2%) after 24 h of incubation, indicating that the HPL1A cell culture model can be used to study the effects of AM and DEA independently. Apoptotic cell death was assessed by annexin-V-FITC (ann-V) staining and by terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick-end labeling (TUNEL), while necrotic cell death was determined by propidium iodide (PI) staining. The percentage of PI positive cells increased over six-fold after 24 h treatment with 20 μM AM (80.8%) compared to control (12.0%), and doubled after 24 h treatment with 3.5 μM DEA (20.4%) compared to control (10.8%). The percentage of ann-V positive cells decreased from 8.26% (control) to 1.56% following 24 h treatment with 10 μM AM and more than doubled after 24 h incubation with 3.5 μM DEA (22.0%) compared to control (9.86%) (p<0.05). Treatment for 24 h with 5.0 μM DEA caused the percentage of TUNEL positive cells to increase from 4.21% (control) to 26.7% (p<0.05). Vitamin E (5 – 20 μM) did not protect against AM or DEA cytotoxicity, as determined by ann-V and PI dual staining.
Angiotensin II (100 pM – 1 μM) alone or in combination with AM or DEA did not alter cytotoxicity. Furthermore, the angiotensin converting enzyme inhibitor captopril did not protect against AM or DEA cytotoxicity. In conclusion, in vitro, AM activates primarily necrotic pathways, whereas DEA activates both necrotic and apoptotic pathways, and the RAS does not seem to be involved in AM or DEA cytotoxicity in HPL1A cells. Multiple mechanisms may contribute to the initiation of lung damage observed clinically, due to actions of both AM and its metabolite DEA.
Keywords: amiodarone, desethylamiodarone, vitamin E, renin-angiotensin system / Thesis (Master, Pharmacology & Toxicology) -- Queen's University, 2009-11-26 13:57:09.65
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A New Bioassay Method for Measurement of Angiotensin II Using Isolated Bovine Adrenal Cells : Clinical Application on the Plasma Renin ActivityYOGO, HIROSHI, TATEMATSU, AKIRA, NONOYAMA, MASUO, TANAKA, MASANORI, KAKO, TAKESHI 11 1900 (has links)
No description available.
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Helicobacter pylori associated effects on inflammatory radical formation and angiotensin II receptors in the stomach /Elfvin, Anders, January 2007 (has links)
Diss. (sammanfattning) Göteborg : Göteborgs universitet, 2007. / Härtill 5 uppsatser.
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Comparative molecular analysis of the binding between severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and angiotensin converting enzyme 2(ACE2)Lam, Chun-yip, January 2007 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2007. / Also available in print.
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Wege der Angiotensin-II-induzierten Apoptose in ventrikulären Kardiomyozyten der adulten Ratte /Schröder, Dunja. January 2008 (has links)
Zugl.: Giessen, Universiẗat, Diss., 2008.
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Wege der Angiotensin-II-induzierten Apoptose in ventrikulären Kardiomyozyten der adulten RatteSchröder, Dunja. January 2008 (has links) (PDF)
Zugl.: Giessen, Universiẗat, Diss., 2008.
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On the development of the Angiotensin IV ligands, Norleual and NLE¹-Angiotensin IV, as anti-cancer and wound healing agentsElias, Patrick David, January 2008 (has links) (PDF)
Thesis (Ph. D.)--Washington State University, August 2008. / Includes bibliographical references.
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