The spread amongst humans of viral diseases such as acquired immunodeficiency syndrome (AIDS), hepatitis and severe acute respiratory syndrome (SARS) is alarming. A plant-based high fidelity production system is being developed with emphasis on producing antigens capable of being orally delivered to humans in plant packets. To test whether transgenic tobacco, carrot and rice plants can correctly process and assemble the hepatitis-B virus (HBV) core particle/antigen (HBcAg), they were transformed with a C-terminal truncated version of the HBcAg subunit coding sequence. Transgenic tobacco, carrot and rice plants processed the HBV subunits accurately indicating that these recombinant expression systems can be extended to produce other proteins at reduced costs. In the wild-type expression construct (H1); the enhanced cauliflower mosaic virus double 35S (CaMV-d35S) promoter was fused to the alfalfa mosaic virus RNA 4 (AMV-RNA4) sequence to achieve greater translation of a C-terminal truncated HBV core particle subunit. A second expression construct (H2) was plant-codon optimized to match the Arabidopsis thaliana plant genome codon preferences. A third codon-optimized expression construct (H3) had a KDEL (lysyl-aspartyl-glutamyl-leucine) encoded sequence. While a fourth expression construct (H4) included an extensin signal sequence in place of the AMV-RNA4 sequence. Western blotting analysis showed the presence of the HBcAg in transgenic tobacco, carrot and rice plants. The HBcAg levels increased from the H1 to the H4 transgenic tobacco lines. Plant codon-optimization of the HBcAg sequence and addition of the KDEL encoded sequence led to higher levels of HBcAg. The most effective modification was observed when the extensin signal sequence replaced the AMV-RNA4 translation enhancer sequence resulting in the highest observed yields of HBcAg in both the leaves and seeds of the best H4 tobacco plant. In edible plants, higher levels of HBcAg were observed in carrot roots as opposed to carrot leaves and in rice seeds as opposed to rice leaves. Further analyses via electron microscopy indicated that the HBV subunits had assembled into virus-like particles of 25--30 nm diameter in all three plant systems. Therefore, these studies may aid in the global quest to develop cheap, safe and effective vaccines.

Master of Science / Department of Animal Sciences and Industry / Micheal J. Brouk / An evaluation of short-term replacement of corn silage (CS) and soybean meal with a blend of wet brewers grains (BG) and cracked corn on milk production and DMI was completed utilizing 8 primiparous (192 DIM) and 4 multiparous (191 DIM) mid-lactation Holstein cows. Milk production, composition, DMI, production efficiency, fatty acid composition and diet digestibility were evaluated. Cows were allotted to a 4 x 4 Latin Square with 3 replications blocked by parity, DIM and energy corrected milk (ECM). Crude protein and starch levels were balanced between diets by varying the levels of cracked corn and soybean meal in four diets; 0 BG (0% wet BG and 24% CS of diet DM), 12 BG (12% wet BG and 12% CS), 18 BG (18% wet BG and 6% CS), and 24 BG (24% wet BG and 0% CS). Fifteen day periods were used, d11-15 designated for collection. Orts were collected daily and TMRs were fed at 5 to 10% of previous day’s intake. Cows were milked 3x/day and individual milk weights recorded at every milking. Milk samples, body weights and BCS were taken -2 and -1d pre-trial to obtain baseline data and d14 and 15 of each period. During collection, samples of TMR and orts were taken d1, 3 and 5. Fecal grab samples were taken d12-15 at 8 hr intervals and advanced 2 hrs every 24 hr period to account for diurnal variation. Dry matter intake was similar (P=0.33) among treatments (20.3, 20.8, 20.9 and 21.2 kg/cow) for 0 BG, 12 BG, 18 BG AND 24 BG respectively, however CP intake of 24 BG tended to be greater (P=0.05) than 0 BG. NDF intake was lower for 0 BG compared to all other treatments and 24 BG was higher than 12 BG (P=0.0007). Dietary fat intake was different (P<0.001) across all treatments, increasing with greater BG inclusion. Inclusion of BG had no effect (P=0.37) on milk production (30.5, 31.5, 31.6 and 32.1 kg/cow), fat percent or amount, protein percent, SNF, lactose or SCC, but protein yield (P=0.04) was lower and MUN (P=0.05)tended to be lower with 0 BG compared to 18 BG and 24 BG. Efficiency of milk production did not differ (P=0.93) among treatments. Milk fatty acid profiles were different among treatments, with general increases of individual fatty acids as BG inclusion increased. No differences were found in DM, CP or ADF digestibility across treatments. Results suggest wet BG fed simultaneously with grass hay can be utilized as a short-term replacement for CS in mid-lactation dairy cow diets.

Master of Science / Department of Animal Sciences and Industry / Barry J. Bradford / The transition from gestation to lactation requires numerous physiological and metabolic adaptations in order for the body to maintain relative homeostasis. For the modern dairy cow, the difficulty to meet these challenges is increased many-fold due to the large demand for energy and metabolites placed on the body by the high producing mammary gland. Milk fever or periparturient hypocalcemia can be defined as a failure of the calcium homeostatic mechanisms to maintain serum calcium around the time of calving. Though clinical cases may only arise in ≈ 5% of transition cows, subclinical rates are much higher. Animals suffering from even subclinical milk fever are much more susceptible to numerous other transition disorders. Preventing milk fever by formulation of the prepartum ration may be accomplished by decreasing the dietary cation anion difference (DCAD) which can be defined as the balance between positively and negatively charged ions in the diet. An experiment was designed to test 2 diets containing t products designed to deliver supplementary anions to the diet versus a control ration with no added anions. Total serum calcium and incidence of postpartum health disorders were not affected by prepartum dietary treatment. Though DCAD was drastically different between the control ration and the 2 anionic diets, the concentration of the strong cation potassium was low across all treatments which presumably prevented hypocalcemia with the onset of lactation. Though our diets contained low concentrations of potassium, many diets used by dairymen contain forages that are high in potassium and thus might benefit from the addition of anions. An experiment of an unrelated nature was conducted to observe the effects of 2 diets containing wet corn gluten feed (46 or 56% of DM) as the primary energy substrate and tallgrass prairie hay (14 or 20% of DM) as the sole source of physically effective fiber versus a control ration containing alfalfa and corn silage. The 20% tallgrass prairie hay diet resulted in milk components and efficiencies similar to those of the control ration, but production and income over feed cost did not match that of the control ration in this situation.

Master of Science / Department of Clinical Sciences / Johann F. Coetzee / Objective—To determine the pharmacokinetic parameters of xylazine, ketamine, and butorphanol administered IM and sodium salicylate administered PO and to compare their effect on biomarkers of pain and distress following sham (Period 1) and actual (Period 2) castration and dehorning. Animals—40 Holstein bull calves Procedures—Calves weighing 108 to 235 kg received the following treatments prior to sham castration and dehorning (Period 1) and castration and dehorning (Period 2) (n=10 calves/group): (i) 0.9% saline solution IM (PLACEBO) (ii) sodium salicylate (SAL) supplied free-choice in water to provide concentrations from 2.5 to 5 mg/mL beginning 24 hours prior to Period 1 to 48 hours after Period 2; (iii) 0.025 mg/kg butorphanol, 0.05 mg/kg xylazine, 0.1 mg/kg ketamine co-administered IM immediately prior to both periods (XKB); and (iv) a combination of treatments (ii) and (iii) (SAL + XKB). Plasma drug concentrations, average daily gain (ADG), chute exit speed, serum cortisol concentrations and electrodermal activity (EDA) were evaluated. Results—ADG (0-13d) was significantly greater in the SAL and SAL + XKB groups. Calves receiving XKB had significantly slower chute exit speed in both periods. Serum cortisol concentrations were significantly increased in all groups during Period 2 compared to Period 1. However, XKB attenuated serum cortisol response for the first hour after castration and dehorning while oral salicylate significantly reduced cortisol from 1-6 hours. XKB administration significantly decreased EDA scores in both periods. Conclusions and Clinical Relevance—Free-choice sodium salicylate decreases cortisol concentrations and reduced weight loss associated with castration and dehorning in calves.

Master of Science / Department of Animal Sciences and Industry / R. Scott Beyer / The modern poultry industry uses auger feed lines to transport feed for broilers to pans to make feed available for them while greatly reducing labor costs. The feed given to broilers is manufactured at significant cost into a pelleted form. Pellets reduce broiler production costs by increasing feed intake and growth rate while reducing feed. Pellet quality is expressed using a method called the Pellet Durability Index (PDI). A series of studies was conducted to determine the effects of these destructive forces on both pellet quality and nutritive value. In Experiment 1, it was found that pellets of 78 and 86 PDI had 30% more fines in the final pan compared to the initial pan, but there was no significant difference between the two diets. In Experiment 2, a significant difference existed between a 23 PDI diet and an 82 PDI diet in the percentage of fines found at 12 selected pans. This indicates destruction occurring along the length of the feed line. In Experiment 3, soybean oil was applied to the exterior of the pellets and they were transported through the feed line. A significant difference in the amount of fat at each collection site was found, as the fat was removed from the exterior of the pellets in earlier pans, indicating destructive forces having an effect on the pellets. In Experiment 4, whole sorghum was used to create four diets with similar PDI. The feed was then placed in the feed line, collected at twelve locations, and analyzed for crude fat, CP, DM, ash and Ca. The results indicated the presence of whole sorghum in the pellets had no effect on percent fines or nutrient values. In Experiment 5, three diets with different PDI were transported through the feed line. Five collection sites were selected. Results indicate that high quality pellets withstand handling while pellets below 68 PDI show increased fine percentage. These experiments indicate pellets are damaged during transport in the feed line, but nutrients remain the same unless they are on the exterior of the pellet.

Master of Science / Department of Animal Sciences and Industry / Daniel W. Moser / The purpose of this study was to estimate the heritability of calving rate and age at first calving in Hereford heifers, and evaluate whether age at calving would add accuracy to a genetic evaluation of calving rate. Pedigree and performance data on Hereford heifers born between 2001 and 2007 was provided by the American Hereford Association. After editing to exclude animals that did not fit inclusion criteria, the evaluated dataset contained 94,709 heifers with calving status information. Data were analyzed using single and two-trait animal models to obtain heritability estimates, and genetic correlation between calving rate and age at first calving was determined using MTDFREML. Contemporary groups for calving traits were defined as heifers that were in the same yearling weight contemporary group, and remained in the ownership of the same breeder through the age that they would be expected to calve. Estimates of heritability for calving rate and age at first calving from single-trait models were 0.25, and 0.12, respectively. Genetic correlation between calving rate and age at first calving was -0.01. Calving rate is moderately heritable in Hereford heifers, and can be used in genetic evaluation of sires to improve the trait through selection. Age at first calving has minimal genetic relationship to calving rate, and is not useful in increasing accuracy of selection for calving rate.

Master of Science / Department of Grain Science and Industry / Leland McKinney / Three experiments were conducted to evaluate value added uses for dried distillers grains with solubles (DDGS) and crude glycerol in swine and poultry diets. In Exp.1, crude glycerol from multiple biodiesel production facilities was evaluated for storage and variability characteristics. Part one reviewed the storage capabilities of crude glycerol at room temperature and excessive heat conditions on ferrous and stainless steel metal. There was no notable corrosion during the two-month experiment for either metal type under each condition. There was a slight discoloration observed on the ferrous metal stored under excessive heat conditions, but no pitting or sign of corrosion was noted. No changes were observed in the stainless steel under either environment condition. Part two evaluated the variability of crude glycerol from multiple biodiesel production facilities. Representative samples of the parent feedstock and resultant glycerol was collected an analyzed. There was considerable variation between samples, particularly when comparing glycerol from the different feedstocks (vegetable vs. animal). Exp. 2 and 3 took place in the Feed Processing Research Center in the Department of Grain Science at Kansas State University. Pellet quality and electrical energy consumption was evaluated by reviewing production rate, conditioning and hot pellet temperatures, motor load, and pellet durability index (PDI) as testing parameters. In Exp. 2, diets containing varying levels of crude glycerol were evaluated on pellet quality and pellet mill performance in a pilot mill and in a commercial facility. For part one, a corn-soy based swine grower diet was formulated to contain 0, 3, 6, and 9% crude glycerol. Each diet was steam conditioned to 150, 170 and 190 °F in an atmospheric conditioner and pelleted. An interaction existed between glycerol and conditioning temperature. For all diets containing glycerol, roll skid occurred and the pellet mill plugged as conditioning temperature approached 190°F. Pellet quality increased linearly (P < 0.01) with increasing levels of glycerol. Part two took place at Don’s Farm Supply in Newell, IA. A corn-soy based turkey grower diet was formulated to contain 3% glycerol and pelleted. Results from part two were consistent with part one, in that there was an interaction between conditioning temperature and the addition of glycerol. Exp. 3 evaluated DDGS on pellet quality and electrical consumption. A poultry diet was formulated to contain 0, 15, or 30% DDGS and steam conditioned to 140, 160, and 180°F. As conditioning temperature increased, pellet quality in diets containing DDGS significantly improved (P>.001). Electrical consumption in diets containing DDGS compared to the control showed no significance in reduction in energy usage (P>.001). In conclusion, the production of pelleted diets containing crude glycerol is a feasible option; however, conditioning temperatures should be kept minimal and storage and variation of the material should be considered. In addition, improved pellet quality in pelleted diets containing DDGS is in part by conditioning at higher temperatures, respectively. The data suggests that the addition of crude glycerol and DDGS in animal diets can serve as a beneficial feed additive.

Doctor of Philosophy / Department of Animal Sciences and Industry / Joe D. Hancock / Twelve experiments were completed to evaluate corn- and sorghum-distillers dried grains with solubles (DDGS) in diets for nursery and finishing pigs. In Exp. 1, corn-DDGS had 223 kcal/kg greater DE than sorghum-DDGS (P<0.02). In Exp. 2, pigs fed a corn-soy control diet had greater ADG, nutrient digestibility, HCW, and dressing percentage (P<0.02) and lower iodine value (IV) of jowl fat (P<0.001) than pigs fed diets with 40% DDGS. High-energy DDGS supported lower ADG, ADFI, and digestibility of DM (P<0.06) than moderate-energy DDGS, and sorghum-DDGS resulted in lower IV than corn-DDGS (P<0.001). In Exp. 3 and 4, addition of sodium bicarbonate to adjust dietary pH and electrolyte balance did not improve growth performance in nursery or finishing pigs (P>0.16) and adding molasses to improve palatability decreased (P<0.05) G:F for finishing gilts. In Exp. 5 and 6, enzyme additions improved nutrient digestibility in nursery (P<0.04) and finishing (P<0.01) pigs fed diets with high inclusion of DDGS. In Exp. 7, expander processing improved (P<0.02) ADG, G:F, and digestibility of DM, N, GE, and cellulose compared with standard steam conditioning of diets for nursery pigs, with the greatest response in G:F for pigs fed sorghum-DDGS (DDGS source × conditioning; P<0.02). In Exp. 8 and 9, expander conditioning improved G:F and dressing percentage (P<0.007) and digestibility of DM, N, and GE compared with standard conditioning (P<0.02), with the greatest response in digestibility of DM for the DDGS diets (diet × conditioning, P<0.01). In Exp. 10, 11, and 12, increasing tallow from 0 to 5% in diets with 40% DDGS improved (P<0.05) G:F. Dressing percentage was improved with addition of tallow and palm oil (P<0.08), but IV become worse (linear, P<0.06) as tallow was increased in the diet. Adding coconut oil improved growth performance and carcass firmness in pigs fed diets with 40% DDGS (P<0.01). In conclusion, growth performance and nutrient digestibility decreased with addition of DDGS to diets for nursery and finishing pigs. However, adding enzymes partially restored nutrient digestibility; expander conditioning improved G:F, dressing percentage, and nutrient digestibility; and adding coconut oil to diets with 40% DDGS improved G:F and carcass firmness.

Master of Science / Department of Grain Science and Industry / Leland McKinney / Carryover of medicated feed additives between batches of feed can potentially result in harmful drug residues in the edible tissues of food-animals. Flushing the equipment with an ingredient, such as ground grain, is one method used to remove any residual medicated feed from the system. It is generally recommended that the quantity of flush used be between 5 and 10% of the mixer's capacity. However, there is little data that supports this recommendation. Therefore, two experiments were conducted to 1.)determine which manufacturing equipment is the major source of carryover, 2.)evaluate which flush size adequately prevents drug carryover, and 3.) quantify the interrelationship between flush size and drug concentration. In Experiment 1, feed medicated with nicarbazin (Nicarb 25%®; 0.0125%) was manufactured and conveyed from the mixer, through a drag conveyor and bucket elevator, and then into a finished product bin. The system was then flushed using ground corn in the amount of 2.5, 5, 10, 15, or 20% of the mixer's capacity (454.5 kg). Subsequently, a non-medicated diet was conveyed through the system and samples were collected and analyzed for nicarbazin. No significant (P > 0.05) differences were detected among the flush treatments, and all treatments were effective in preventing nicarbazin carryover to the non-medicated diet. In Experiment 2, feed medicated with three levels of monensin (Rumensin® 80; 100, 600, and 1,200 g/ton) was manufactured and handled in the same manner as in Experiment 1. The flushing treatments examined were: 1, 2.5, and 5% of the mixer's capacity. Samples of the non-medicated diet for each treatment were collected and analyzed for monensin. There was significant interaction (P < 0.05)between drug level and sampling location between treatments. As the drug level in the medicated diet increased, higher concentrations of monensin were detected in the non-medicated diet. Collectively, these studies demonstrate that a 2.5%, even a 1% flush size, is effective in preventing carryover of medicated feed additives. It was also demonstrated that the bucket elevator and finished product bin were the major sources of drug carryover in this particular feed manufacturing system.

Master of Science / Department of Animal Sciences and Industry / Dale A. Blasi / An experiment was conducted to evaluate the effects of dry matter intake (DMI) restriction on early receiving performance by steers in a drylot and subsequent grazing performance, feedlot performance, and carcass characteristics. During the backgrounding period, crossbred, weanling steers (n = 329; initial BW = 191± 5.52 kg ) were randomly assigned to 1 of 4 DMI levels corresponding to ad libitum, 2.50% of BW (2.50%), 2.25% of BW (2.25%), and 2.00% of BW (2.00%) for 62 d. During the subsequent grazing period, the same steers were randomly assigned to 13 paddocks to graze for 90 d. Paddocks were stocked at 281 kg live weight per hectare. Initial steer BW were similar on each pasture and each backgrounding treatment was equally represented within a paddock. During the feedlot period, steers were finished at a commercial feedlot and were assigned to 1 of 4 pens according to their rank in BW. Entire pens were harvested when average steer BW reached 545 kg. During the backgrounding period, ad libitum-fed steers had greater (P < 0.001) ADG and final BW than other treatments; steers fed at 2.50 and 2.25% of BW had similar ADG and final BW and were greater (P < 0.001) than steers fed 2.00% of BW. During the grazing period, compensatory gain was observed in restricted DMI treatments. Steers fed at 2.00% of BW had greater (P = 0.006) ADG than ad libitum-fed steers but an ADG similar to that of the other restricted DMI treatments. Steers fed ad libitum, 2.50% of BW, and 2.25% of BW had similar final BW and steers fed 2.00% of BW had lesser (P < 0.001) final BW than other treatments. During the feedlot phase, steers fed 2.00% of BW were on feed longer (P < 0.05) than other treatments. Growth compensation during grazing illustrated that restricted feeding immediately prior to pasture grazing can reduce backgrounding costs.