Thesis (M.S.)--University of Tennessee, Knoxville, 2008. / Title from title page screen (viewed on Mar. 9, 2009). Thesis advisor: J. Scott McElroy. Vita. Includes bibliographical references.

Annual bluegrass (Poa annua L.) is a problematic weed in turfgrass that has evolved resistance to twelve different herbicide sites of action. The mitotic-inhibiting herbicide pronamide has both pre- and post-emergence activity on susceptible annual bluegrass populations. Still, post-emergence activity may be compromised in some resistant populations due to the lack of root uptake or an unknown foliar resistance mechanism. Spray droplet size may affect foliar and soil deposition of pronamide, thus potentially explaining variation in population control or differential foliar and root uptake. Pronamide, flazasulfuron, and pronamide + flazasulfuron deposition were quantified on annual bluegrass as affected by spray-droplet size. The efficacy of these herbicide treatments in resistant (R) and susceptible (S) annual bluegrass populations was then evaluated with two droplet sizes (400 and 1000 μm). Absorption and translocation of pronamide were investigated in R and S populations following foliar-only and soil-only pronamide applications.

No description available.

Annual bluegrass weevil (Listronotus maculicollis Kirby) (Coleoptera: Curculionidae) (ABW) is a major pest of annual bluegrass (Poa annua L.) and creeping bentgrass (Agrostis stolonifera L.) on golf courses in the northeastern United States. The asynchronous life cycle makes managing ABW difficult, putting emphasis on scouting to achieve accurate insecticide timing and acceptable control. Little is known about the biology and management of ABW in Virginia's more temperate climate. Reported cases of ABW resistance to pyrethroids (IRAC Group 3) continues to grow in the northeast, yet no pyrethroid-resistance cases have been reported in Virginia outside of the metropolitan Washington, D.C. For this thesis, I confirmed the widespread distribution of ABW across Virginia with a survey of golf course superintendents. Two golf courses in southwestern Virginia were monitored weekly during the 2019 and 2020 growing seasons to determine the seasonal biology of ABW within this region. These data suggest that overwintering ABW emerge much earlier than described in the northeast, with adult weevil activity beginning in late February or early March. I observed three complete ABW generations, with a potential fourth generation. Soil plugs from the same two golf courses were used to compare the salt floatation and Berlese-Tullgren funnel methods of larval extraction. The methods were highly correlated (R2 = 0.7856), suggesting either method is appropriate for ABW larval extraction. Bioassays conducted on adult ABW from the same two golf courses showed that field rate concentrations of the pyrethroid bifenthrin showed variable mortality ranging from 20% to 80% suggesting the presence of resistance genes in the population. A 100-fold rate of bifenthrin resulted in 100% mortality of ABW, however. Because cross-resistance has been reported among northeastern ABW populations, the common insecticide active ingredients chlorpyrifos (IRAC Group 1B: Organophosphate), trichlorfon (IRAC Group 1B: Organophosphate), λ-cyhalothrin (IRAC Group 3: Pyrethroid), α-cypermethrin (IRAC Group 3: Pyrethroid), imidacloprid (IRAC Group 4A: Neonicotinoid), and spinosad (IRAC Group 5: Spinosyn), were tested on ABW adults. In another bioassay, two organophosphates, trichlorfon and chlorpyrifos, resulted in significantly higher ABW mortality rates than all other labeled insecticides (P < 0.0001). Two other larvicides, spinosad and α-cypermethrin, also exhibited adult control, an important factor to consider for ABW management and preventing pyrethroid-resistance. These results provide valuable insight into the seasonal biology and management of ABW in Virginia and direction for further investigation into these populations. / Master of Science in Life Sciences / Annual bluegrass weevil (Listronotus maculicollis Kirby) (ABW) is a tiny, but damaging insect pest of cool-season golf course turfgrasses in the northeastern United States. As pest populations have spread southward, ABW has become an emerging pest in Virginia. The objectives of this thesis were to 1) determine the geographic distribution and seasonal biology of ABW in Virginia, 2) compare two methods for extracting ABW larvae from turf cores in order to estimate larval densities, and 3) to assess the susceptibility of ABW to various insecticides commonly used by golf course superintendents. A survey of golf course superintendents in 2019 revealed widespread prevalence of ABW across Virginia, particularly in areas growing predominantly cool-season turfgrasses. Two golf courses in southwestern Virginia were monitored weekly during the growing seasons of 2019 and 2020 to determine the seasonal biology of ABW. Data suggest that overwintering ABW emerge much earlier than reported in the northeast, with adult weevil activity beginning in late February or early March compared to April in the northeast. In addition, I detected three complete ABW generations, with a possible fourth generation occurring. Soil plugs from two golf courses were used to compare a heat extraction method using a Berlese-Tullgren funnel with the traditional salt float method for extraction of ABW larvae. The two methods extracted similar numbers of ABW larvae suggesting that either method is appropriate for ABW larval extraction. Adult ABW from the same two golf courses were collected and subjected to the pyrethroid insecticide bifenthrin at 0.128 kg ai ha-1 (field application rate), 1.28 kg ai ha-1 (10-fold rate), 12.8 kg ai ha-1 (100-fold rate), along with a water control. Results indicated that the field application rate of bifenthrin killed > 50% but <90% of ABW adults, suggesting that some resistance may be present in the population. A concentration of 100-fold bifenthrin field rate killed 100% of tested individuals, suggesting that widescale field resistance to pyrethroids is likely not present. Because cross-resistance has been reported among northeastern ABW populations, the common insecticide active ingredients chlorpyrifos (IRAC Group 1B: Organophosphate), trichlorfon (IRAC Group 1B: Organophosphate), λ-cyhalothrin (IRAC Group 3: Pyrethroid), α-cypermethrin (IRAC Group 3: Pyrethroid), imidacloprid (IRAC Group 4A: Neonicotinoid), and spinosad (IRAC Group 5: Spinosyn), were tested on ABW adults from the same two golf courses. Two organophosphates, trichlorfon, a larvicide, and chlorpyrifos resulted in significantly higher ABW mortality rates than all other active ingredients (P < 0.0001). Two other larvicides, spinosad and α-cypermethrin, also exhibited adult control, an important factor to consider when attempting to manage ABW and preventing resistance to pyrethroids. Our results provide valuable insight into the seasonal biology and management of ABW in Virginia and direction for further investigation into these populations.

Annual bluegrass is a winter annual grass that is problematic on golf putting greens due to its light green color, prolific seedhead production and intolerance to stress. On creeping bentgrass putting greens, herbicides for annual bluegrass control are limited. A new herbicide, methiozolin, developed by Moghu Research Center, LLC, in Daejeon, South Korea, safely and selectively controls annual bluegrass in creeping bentgrass and several other turfgrass species. Methiozolin typically controls annual bluegrass over several weeks, allowing desirable turfgrass time to grow into areas previously infested by annual bluegrass with little surface disruption. The mode of action of methiozolin is unknown, but has been proposed to act as either a cell wall biosynthesis inhibitor (CBI) or an inhibitor of tyrosine aminotransferase (TAT). Field studies were conducted at Virginia Tech to investigate strategies promoting surface recovery on putting greens following atypically rapid annual bluegrass loss resulting from methiozolin application, intensive core-cultivation as well as potential interactions with plant growth regulators (PGR's), like ethephon. In the rapid annual bluegrass removal study, all treatments receiving additional fertility via synthetic fertilizer with or without trinexapac-ethyl or biostimulant recovered 1 to 3 weeks more quickly than treatments that did not include additional fertility. Addition of the PGR trinexapac-ethyl inconsistently regulated speed of canopy recovery, both increasing and decreasing recovery speed. Under normal maintenance conditions, methiozolin does not negatively influence putting green recovery, however, if the putting green is exposed to droughty conditions, methiozolin can reduce recovery time by several weeks. Core-cultivation should be avoided in conjunction with methiozolin and ethephon applications because when this procedure was conducted on the same day as herbicide application it significantly damaged creeping bentgrass, reducing cover to 19% at 2000 g ai ha⁻¹, compared to the non-treated at 62%. Regarding the question of methiozoling mode of action, laboratory studies supported the claim that addition of exogenous 4-hydroxyphenylpyruvate (4-HPP) alleviates symptoms of methiozolin exposure in lesser duckweed, a model monocot species, but feeding various turfgrass species and annual bluegrass exogenous 4-HPP did not alleviate symptoms. Creeping bentgrass secondary root length and density was not affected by methiozolin, although annual bluegrass, Kentucky bluegrass and perennial ryegrass secondary root lengths were reduced. Based on these data, it does not appear that TAT inhibition is a primary mode of action of methiozolin in turfgrass. Studies were conducted to determine if methiozolin inhibited cell wall biosynthesis in desirable turfgrass species and annual bluegrass. All species exhibited decreased enrichment of ¹³C in cell-wall sugars form ¹³C-glucose in response to methiozolin and a known cell wall biosynthesis inhibitor, indaziflam. Indaziflam and methiozolin at 0.01 µM inhibited ¹³C enrichment of all sugars less than methiozolin at 1.0 µM, for xylose, arabinose and glucose, but not galactose. Addition of 4-HPP increased incorporation of ¹³C into xylose, but had no other influence on ¹³C incorporation into other cell wall sugars. Lack of species specific response indicates that cell wall biosynthesis inhibition is probably not the source of interspecific species responses observed in the field. / Ph. D.

Annual bluegrass (Poa annua L.) is the most problematic weed to control in creeping bentgrass (Agrostis stolonifera L.) putting greens.  The objective of this study was to transition a mixed putting green stand of annual bluegrass and creeping bentgrass to a monoculture by using fertilizers and plant growth regulators that selectively inhibit annual bluegrass.  A 25 year old loamy sand rootzone research green, planted with \'Penn-Eagle\' creeping bentgrass, with roughly 45% initial annual bluegrass coverage was utilized.  The biweekly application of ammonium sulfate (4.8 kg ha-1) with treatments of ferrous sulfate at rates of 0, 12.2, 24.4, and 48.8 kg ha-1 and in combination with seaweed extract (12.8 L ha-1) or paclobutrazol (0.37 L ai ha-1 spring and fall; 0.18 L ai ha-1 summer) were applied March to October, 2011 and 2012.  Plots receiving the highest rate of ferrous sulfate resulted in annual bluegrass infestation declines from an early trial amount of 45% to a final average of 20% but also resulted in unacceptable late-summer events of annual bluegrass collapse.  The ferrous sulfate medium rate resulted in a smooth transition from early-trial annual bluegrass infestation of 45% to an end of trial infestation of 20% and had the highest putting green quality.  Previous research has reported that consistent use of paclobutrazol can effectively and safely reduce annual bluegrass infestations.  In this trial annual bluegrass was reduced to 9% infestation after three months of application.  Two unexpected observations from this trial were that ferrous sulfate, applied at medium to high rates, significantly reduced silvery thread moss (Bryum argentum Hedw.) populations and occurrences of dollar spot (Sclerotinia homoeocarpa F. T. Bennett) disease.  Dollar spot control with ferrous sulfate has not previously been reported in the literature, so additional studies were designed to investigate this phenomenon further.  A creeping bentgrass putting green study was conducted to determine if sulfur, iron, or the two combined as ferrous sulfate decreases dollar spot activity.  Ferrous sulfate resulted in the highest turf quality and suppressed S. homoeocarpa infection, even during high disease pressure.  Fe-EDTA suppressed dollar spot infection as well as ferrous sulfate but quality declined to unacceptable levels during the summer, due to Fe-EDTA only.  Sulfur did not affect or increased S. homoeocarpa infection, indicating that a high and frequent foliar rate of iron is responsible for dollar spot control.  An in-vitro study was conducted to determine if agar pH in combination with iron concentrations affects mycelial growth of S. homoeocarpa.  Results from this trial indicated that 5.4 agar pH is an optimal pH for mycelial growth.  The 10 to 100 mg iron kg-1 concentration had little effect on mycelial growth at 5.0 and 5.5 pH, but increased growth at 4.5 and 6.5 pH.  As the iron concentration was increased from 10 to 100 to 1000 mg kg-1, mycelial growth decreased or stopped.  Our final conclusions are that seasonal biweekly foliar applications of the medium rate of ferrous sulfate (24.4 kg ha-1) safely and effectively reduced annual bluegrass infestation out of a creeping bentgrass putting green, while also effectively suppressing silvery thread moss and dollar spot incidence. / Master of Science

Golf courses can expand hundreds of acres, making scouting for both pests and beneficial insect populations a time-consuming task. Scouting for insects is labor-intensive, potentially damaging, but is an integral part of an integrated pest and pollinator management (IPPM) plan. Virginia golf courses are currently using remote sensing and light reflectance to detect non-insect pests in turfgrass. This thesis aims to develop remote sensing and light reflectance methods to aid in a turfgrass IPPM plan, to document the phenology of ABW weevil (Listronotus maculicollis Kirby, Coleoptera: Curculionidae, ABW), and to catalogue pollinator-friendly out-of-play areas. Light reflectance, the measurement of the amount of light reflected, of plants can be used as a proxy for the health of a plant. The light reflectance of turfgrass affected by ABW stress and plants in the out-of-play areas of golf courses was collected proximally and remotely, using a backpack spectrometer and an unmanned aerial vehicle (UAV), respectively. Mathematical light reflectance indices were applied and compared to insect populations in both areas to determine the correlation. The Normalized Difference Vegetation Index (NDVI), which uses red and near-infrared wavelengths to indicate stress, was found to highlight ABW stressed turfgrass. The Structure Intensive Vegetation Pigment Index (SIPI), which uses red and green wavelengths to highlight flowering plants, was found to highlight potential pollinator- friendly habitats in out-of-play areas. When applied to flights, NDVI could help in the targeted application of insecticides to combat the annual bluegrass weevil, therefore reducing their presence in the environment. The use of SIPI could highlight potential pollinator friendly habitats and therefore assist superintendents in the development of their IPPM plan. / Master of Science in Life Sciences / Scouting, such as completing visual monitoring or taking soil core samples, is an important part in the development of an integrated pest and pollinator management (IPPM) plan for Virginia golf courses; an IPPM plan focuses on control of a pest, while considering the needs of pollinators. The size of golf courses makes scouting for insect pests and beneficial insects a time-consuming task. Golf courses are currently using remote sensing, the use of drones in combination with other technology, to scout for other pests or disease. Light reflectance, the measurement of the amount of light reflected, is often used in combination with remote sensing as a proxy for the health of plants. This thesis developed remote sensing and light reflectance techniques not only to detect a common turfgrass pest, the annual bluegrass weevil (Listronotus maculicollis Kirby, Coleoptera: Curculionidae, ABW), but to also predict the presence of potential pollinator habitats in the out-of-play areas of Virginia golf courses. Instruments such as a spectrometer and a drone were used to collect light reflectance at the ground level and aerially, respectively. Ground data was collected through soap water flushes to detect adult ABW, and visual monitoring of potentially pollinating bees, beetles, butterflies, and flies. The light reflectance and ground data were compared using mathematical indices to determine if there was a relationship between the presence of insects and a particular index. Indices could be applied to drone flights that golf course superintendents are already performing, and they can use this information to highlight potential areas of insect presence. This will help them to take care not to apply insecticides in areas with pollinators or to only apply necessary insecticides where there is likely a presence of ABW. This will reduce the labor, other costs, and the environmental impact of insecticides.

<p>  </p> <p>Annual bluegrass (<em>Poa annua</em> L.) is the most troublesome weed in turfgrass systems and the second most troublesome weed across all grass crops. Controlling annual bluegrass is exceptionally complicated due to its high genetic adaptability to new environments. Additionally, prolific seed production allowed the rapid development of herbicide resistance to 12 herbicide modes of action. Experiments were initiated with the goal to better understand annual bluegrass ecology and resistance to ethofumesate. A dose-response experiment was initiated in 2022 to determine the potential level of ethofumesate resistance in annual bluegrass collected from seed production systems. Seed from 55 annual bluegrass populations was obtained from three sources: seed production fields (31 populations), seed cleaning process (6 populations), and seed testing prior to retail distribution (18 populations). </p> <p>Individual seedlings (2–3 tillers) were treated with ten doses of ethofumesate: 0, 0.6, 1.1, 2.8, 5.6, 8.4, 11.2, 16.8, 22.4, and 44.8 kg ai ha−1; with 1.1 to 2.2 kg ha−1 as the label application rates for perennial ryegrass (<em>Lolium perenne</em> L.). The resistance to susceptible ratio of populations across all sources ranged from 0.48 to 5.48. The most resistant populations from production fields, removed during the seed cleaning process, or found in seed testing lots had ED50 values of 12.1, 13.1, and 9.4 kg ai ha−1, respectively. Further, 68% of the populations found in production fields had ED50 higher than 6 kg ai ha−1, which indicates that annual bluegrass resistance is common in grass seed production. A garden study was initiated in November 2020 to assess the development, reproduction, and survival of ten annual bluegrass populations in Indiana. Annual bluegrass plants were maintained in the absence of turf competition and not subjected to typical turfgrass management practices including irrigation, mowing and fertilization. Data collected in included growth rate, biomass production, ground cover, morphology, flowering time, seed production and morphology, and both winter survival and subsequent summer survival of plants. Principal component analysis indicated that certain populations grouped together based on their development, morphology, stress tolerance, and seed production. Plants from the cooler climates (OR, PA, and IN) were characterized by higher growth rates and biomass compared to southern ecotypes. These three populations survived the longest during the summer, with the PA population averaging the highest ground cover of 276 cm2 on 23 July 2021. Plants from warm climates (AL, FL, NC, SC, TN, TX) had poor summer survival. Additionally, the FL population had the highest winterkill of 68%, followed by TX at 45%. The NJ population was distinct from other populations, and plants had robust aboveground biomass and high seed production. The results indicate that the development, reproduction, and survival of different annual bluegrass biotypes are dependent on the climate of origin. A third experiment was designed to understand patterns of germination and seed longevity in populations from five climates across the U.S. at two depths of burial. Seed was retrieved in 6-month intervals up to 24 months. Seed viability by depth (surface vs. 5-cm deep) of burial differed only 18 months after the initiation of the study. However, seed viability did differ among populations on each date of seed retrieval. Viability was low ranging from 0.21 to 0.91%, and populations originating from cool climates (New Jersey, Pennsylvania, and Oregon) generally had higher viability than populations originating from warmer climates. Annual bluegrass seed tested in this study typically had low levels of survival (<0.5%); however, all populations retained some level of viability 24 months after burial, which would allow future reproduction of this troublesome weed. Overall, this research determined that herbicides alone will likely be ineffective at controlling annual bluegrass and that knowledge of the development, reproduction, and survival of local annual bluegrass populations should be factored into an integrated weed management strategies created for each site. </p> <p>  </p>

Annual bluegrass (Poa annua L.) and roughstalk bluegrass (Poa trivialis L.) are among the most troublesome grass weeds on golf courses throughout the United States. Herbicides for selective control of these weeds in cool-season fairways are limited and ineffective. Methiozolin is a new isoxazoline herbicide that controls annual bluegrass on putting greens and shows promise for possible weed control in fairways. Kentucky bluegrass (Poa pratensis L.) is among the most common turfgrass species used for golf fairways in the Northern United States and its response to methiozolin has scarcely been tested. A 2.5-yr field study was conducted at four Virginia locations to evaluate methiozolin efficacy for selective annual bluegrass and roughstalk bluegrass control in creeping bentgrass (Agrostis stolonifera L.) or Kentucky bluegrass fairways. Another study evaluated the response of 110 Kentucky bluegrass varieties to three rates of methiozolin. Annual bluegrass has long been presumed to impact putting green trueness, or the ability of the greens canopy to provide a smooth and directionally-consistent ball roll. Although much research has evaluated the impact of greens management on ball roll distance, no peer-reviewed research has evaluated how canopy surface factors, such as weedy annual bluegrass, will influence ball roll direction. Laboratory and field research was conducted to elucidate and overcome experimental errors that may be limiting assessment of ball directional imprecision caused by greens canopy anomalies. Techniques to minimize experimental error were employed in field studies at two Virginia golf courses to determine the influence of annual bluegrass on ball directional imprecision, bounce, and acceleration. Study results suggest that annual bluegrass patches in a creeping bentgrass putting surface can cause subtle increases in ball directional imprecision and bounce but several sources of error must be controlled before these effects can be measured. By using a mechanical putter to avoid directional errors associated with simulated-putt devices, selecting golf balls with balanced centers of gravity, eliminating legacy or "tracking" effects of repeated ball rolls via canopy brushing, and scoring ball direction 30 cm prior to terminal acceleration, we were able to detect an increase in ball directional imprecision of 8 mm m⁻¹ when balls rolled over a single patch of annual bluegrass compared to adjacent rolls on visually-pure creeping bentgrass. In herbicide efficacy studies, methiozolin-only treatments did not significantly injure creeping bentgrass or Kentucky bluegrass, reduce quality, or reduce normalized difference vegetative index regardless of application timings and rates. In general, fall applications of methiozolin reduced roughstalk bluegrass and annual bluegrass cover more than the spring-only treatments. At 1 year after the last treatment, methiozolin at 1500 g ha⁻¹ applied four times in fall at 2-wk intervals for two consecutive years controlled roughstalk bluegrass and annual bluegrass ≥85% and more consistently than other herbicides or treatment regimes. Spanning 110 Kentucky bluegrass varieties, a commercially-acceptable threshold of 30% Kentucky bluegrass injury required between 3.4 to more than 10 times the methiozolin rate needed for annual bluegrass control. Results indicate that annual bluegrass increases directional imprecision and bounce of golf balls rolling across a greens canopy. Methiozolin could be a viable herbicide for managing annual and roughstalk bluegrass in Kentucky bluegrass and creeping bentgrass fairways but weed control efficacy may be dependent on application timing. By measuring small differences in ball directional imprecision as influenced by greens canopy factors, future research efforts will aim to help turf managers choose appropriate greens management techniques. / Ph. D. / Annual bluegrass and roughstalk bluegrass are among the most troublesome grass weeds on golf courses throughout the United States. Both these weedy bluegrass species reduces the aesthetics and playability of golf turf, including fairways, tees, and putting greens. Since both annual bluegrass and roughstalk bluegrass favors growing conditions very similar to that of desirable cool-season grasses, especially Kentucky bluegrass and creeping bentgrass – the most prominent cool-season grasses on golf courses throughout the Northern USA, selective removal of these weedy bluegrass species from the desirable turf sward is very difficult. Moreover, genetic similarity of annual bluegrass and roughstalk bluegrass to Kentucky bluegrass accentuates the difficulty in selective control even more. Commercially-available herbicides for selective control of these weedy bluegrass species in cool-season golf fairways are limited and often ineffective for long-term control. Methiozolin (PoaCure®) is a new herbicide that has been extensively studied and shown to control annual bluegrass on golf putting greens and shows promise for possible weed control in fairways. However, PoaCure® has scarcely been tested to selectively and safely control annual bluegrass and roughstalk bluegrass in cool-season golf fairways. Therefore, field research was conducted at four Virginia locations to evaluate PoaCure® efficacy for selective, long-term annual bluegrass and roughstalk bluegrass control in creeping bentgrass or Kentucky bluegrass fairways. To assess the weed-control potential of PoaCure® on a broader spectrum of Kentucky bluegrass varieties grown here in VA and other cool-season grass growing parts of the nation, another field research was conducted to evaluate the response of 110 Kentucky bluegrass varieties to three different field application rates of PoaCure®. In PoaCure® weed control efficacy studies, PoaCure® by itself did not injure or reduced quality of creeping bentgrass or Kentucky bluegrass regardless of application timings and rates. In general, fall applications of PoaCure® reduced roughstalk bluegrass and annual bluegrass green cover more than the spring-only treatments. At trial completion, which was 2.5 years after trial initiation and 1 year after the last herbicidal treatment, PoaCure® at 82 fl oz/A applied four times in fall at 2-wk intervals for two consecutive years provided ≥85% control of annual bluegrass and roughstalk bluegrass and did so more consistently than other herbicides or treatment regimes in the study. In the tolerance study of 110 Kentucky bluegrass varieties, a commercially-acceptable threshold of 30% injury required between 3.4 to more than 10 times the PoaCure® rate needed for annual bluegrass control. Results from herbicide efficacy and tolerance studies indicate that PoaCure® could be a viable herbicide for managing annual and roughstalk bluegrass in Kentucky bluegrass and creeping bentgrass fairways but weed control efficacy may be dependent on application timing. In addition to being difficult to control and aesthetically unpleasing to view, annual bluegrass has also long been blamed for missed golf putts. Some researchers have surmised that a golf ball's direction may be altered when the ball traverses an anomaly in the greens surface, such as annual bluegrass, but no scientific studies have tested this assumption. Laboratory and field research was conducted to elucidate and overcome experimental errors that may be limiting assessment of ball directional imprecision caused by greens canopy anomalies. Study results suggest that an isolated patch of annual bluegrass increases ball directional imprecision by 8 mm m^-1 compared to visibly-pure creeping bentgrass and that tools and methodology currently reported in scientific literature are not precise enough to discern these subtle changes in direction. At Virginia Tech, we devised a new methodology to discern subtle changes in ball roll directional precision and bounce as influenced by an isolated patch of annual bluegrass in an otherwise visually-pure creeping bentgrass canopy. We used a mechanical putter to minimize directional errors associated with commercially-available simulated putt-devices, selected balanced golf balls, eliminated legacy of repeated ball rolls by brushing putting green surface canopy between ball rolls, scored ball direction prior to terminal acceleration with pressuresensitive paper, and used high-speed video and motion tracking software to measure ball wobble and bounce. Results indicate that annual bluegrass increases directional imprecision and bounce of golf balls rolling across a greens canopy.