Valová D.: Vinca minor L. alkaloids and their biological activity III. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2020, 75 p. Alzheimer's disease (AD) is a progressive, neurodegenerative disorder and it's the most common form of dementia. Because we're still not able to treat the cause of disease, searching for a new substance is relevant. This thesis is focused on isolation of alkaloids from a Vinca minor L. alkaloidal extract as a potential drug. The preparation and column chromatography of the summary extract were performed by Ing. Miroslav Ločárek as a part of his doctoral studies. Subsequent preparative TLC led to the isolation of three compounds. The chemical structures of the isolated alkaloids were elucidated by means of optical rotation, NMR and MS analyses and by comparison of the obtained data with those in the literature. One of the compounds was determined as(-)-vincine, other two compounds have not been isolated yet. Isolated compounds were also tested for their biological activity. Vincine, DV-1 a DV-3 were tested for their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Additionally, vincine and DV-3 were also tested for their inhibitory activity on prolyl...

Vašíčková A.: Alkaloids of Vinca minor L. and their biological activity (inhibition of human cholinesterases) V. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové 2020. From the selected fraction VM 215-258 were isolated 3 alkaloids by flash chromatography followed, preparative thin layer chromatography and crystallization. Their structure was determined by mass spectroscopy, NMR and optical rotation, and the obtained data were compared with those in literature. Strictamine and minovincinine belong to alkaloids previously isolated, the alkaloid AV-3 has not been isolated yet. These alkaloids were tested for their ability to inhibit human cholinesterases and their inhibitory activity was compared to standards galanthamine and physostigmine. Compound AV-3 showed mild inhibitory activity against BChE (IC50 μM > 86.3 ± 2.3), other alkaloids were considered inactive, their IC50 values against cholinesterases were > 100 μM. Key words: Vinca minor L. (Apocynaceae), vinca alkaloids, minovincinine, strictamine, Alzheimer's disease, acetylcholinesterase, butyrylcholinesterase.

A região amazônica possui grande diversidade de espécies vegetais, sendo considerada uma das maiores do mundo, entretanto, pouca atenção vem sendo dada quando se trata de estudos tecnológicos e morfoanatômicos de sementes de espécies florestais. Com isso, o objetivo deste trabalho foi estudar a germinação, morfologia, anatomia de sementes maduras, plântulas e plantas jovens de Aspidosperma spruceanum, visando buscar conhecimentos biológicos e análise de sementes, que irá propiciar o uso dessa espécie em programas de reflorestamento. Os estudos foram realizados no Laboratório de Sementes Florestais (Embrapa Amazônia Oriental) e no Laboratório de Anatomia Vegetal (Museu Paraense Emílio Goeldi). A metodologia e a terminologia empregada foram específicas de cada área, ilustrando os principais caracteres com gráficos, desenhos, fotografias e imagens. Os resultados tecnológicos mostraram que houve diferenças significativas entre os frutos e as sementes das matrizes analisadas para os dados biométricos, entretanto, o tamanho do fruto não influencia no número de sementes por fruto. A espécie produz fruto relativamente grande, em média de 17 sementes por fruto. A porcentagem de germinação, tempo médio e índice de velocidade de germinação não diferiram significativamente entre as sementes das matrizes, entretanto, as médias mostraram que houve diferença significativa entre as temperaturas e os substratos. O melhor índice de germinação foi obtido na temperatura de 250C, destacando-se o substrato vermiculita. Verificou-se que sementes de A. spruceanum não apresentam dormência. Morfologicamente as sementes são aladas e estenospérmicas, constituída por testa, com germinação do tipo epígea fanerocotiledonar. Apresenta eofilos simples, opostas, pecioladas e metafilos alternos e peciolados. Anatomicamente, as células da testa e da ala são alongadas, heterodimensionais, com presença de mucilagem, e o envoltório interno apresenta células epidérmicas, com 2 a 3 camadas de células parenquimáticas irregulares e cristais do tipo drusa de carbonato de cálcio. Os cotilédones apresentam epiderme adaxial e abaxial unisseriadas, com forma poligonal, heterodimensionais, parede anticlinal espessa, coberta por cutícula delgada incipiente, desprovida de complexo estomático. Pelos resultados obtidos das partes analisadas de sementes de A. spruceanum pode-se concluir um melhor conhecimento biológico da espécie, pelos caracteres específicos e indispensáveis gerados para o seu desenvolvimento. / The amazon region has a big diversity of vegetable, being considered one of the biggest of the world. However, less attention has been given when is about technologics and morphoanatomics studies of forests species seeds. Whin this, the aim of this work was study the germination, morphology, anatomy of mature seeds, seedlings and juvenile plants of the Aspidosperma spruceanum aiming lokk for biologics knowledge and seeds analysis, that will be propritious the use of this species in reforestation programs. The studies were made on Forests Seeds Laboratory (EMBRAPA Amazônia Oriental) and on Vegetable Anatomy Laboratory (Museu Paraense Emílio Goeldi). The methodology and terminology applied were specifics in each area, illustrating the mean characteristics with graperics, draws, photografs and pictures. The technologics results showed that were meaning fue differences between the fruits and the seeds of the analysed matrix for the biometrics basics, however, the size of the fruit doesnT have influence on the number of seeds by fruit. The specie produces fruit relatiraly big, an average of 17 seeds by fruits. The percentage of germination, average time and speed rate of germination don't differed meaning fully among the matrix seeds however, the middle ones showed that was a meanful difference between the temperatura and the substracts. The best rate of germination was made on 250C of temperatura, outstanding the vermiculite substract. It was found seeds of A. spruceanum don't present dormancy. Morphologically the seeds are winged and esternospermics, constituted by forehead, with germination by the fanerocotylar epigeal. Presents eophylls simples, opposite, petiolates and metaphylls are alternate and petiolate. Anatomicaly, testai and winged cells are elongated, heterodimensional, with mucilage presença, and internai coat presents epidermics cells, witer two to three layer of irregular parenquimatics cells and crystal of type drusa and calcium carbonate druses. The cotiledones present adaxial epiderm and abaxial unisseriaded, with polygonal form, heterodimensional cells, consistent anticlinal wall, filled by an incipient thin cuticle, without a stomats complex. By the results got it of the analysed parts of seeds of A. spruceanum can be conclude a better biological knowledgment of the specie, by the specifics characteristics and essential generated for it's deveiopment.

Submitted by Cleide Dantas (cleidedantas@ufpa.br) on 2014-12-10T00:12:58Z No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_EstudoFitoquimicoAtividade.pdf: 2445711 bytes, checksum: 3a6673c71aabe192cc664cb74e214829 (MD5) / Approved for entry into archive by Ana Rosa Silva (arosa@ufpa.br) on 2014-12-12T14:47:26Z (GMT) No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_EstudoFitoquimicoAtividade.pdf: 2445711 bytes, checksum: 3a6673c71aabe192cc664cb74e214829 (MD5) / Made available in DSpace on 2014-12-12T14:47:26Z (GMT). No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Dissertacao_EstudoFitoquimicoAtividade.pdf: 2445711 bytes, checksum: 3a6673c71aabe192cc664cb74e214829 (MD5) Previous issue date: 2014 / O presente trabalho descreve a análise fitoquímica e avaliação da atividade antiplasmódica em Plasmodium falciparum (W2) de Himatanthus articulatus (Vahl) Woodson. O extrato etanólico (EEHS) obtido por percolação do pó das cascas, após concentração, forneceu um precipitado (EEHSP) e um resíduo pastoso que foi submetido a liofilização (EEHS). Fracionou-se este por: re-extração sob refluxo, partição ácido-base e coluna cromatográfica de sílica gel. Além disso, submeteu-se o pó das cascas a extração com ácido clorídrico 1 N para separação de alcalóides. A prospecção fitoquímica, em CCD, foi realizada com EEHS e EEHSP, enquanto que EEHS, frações (FrDCM EEHS, FrAcOET EEHS e FrMeOH EEHS) e FAHS2 DCM foram analisadas por cromatografia líquida de alta eficiência acoplada a DAD (arranjo de diiodo-CLAE-DAD). FrAcOET EEHS foi fracioanda por coluna cromatográfica fornecendo uma substância isolada (S1). Analisou-se o EEHS, a fração majoritária obtida na coluna cromatográfica (F71), FAHS2 DCM e S1 por cromatografia liquida acoplada a espectrometria de massas (CL-EM). O espectro no infravermelho foi obtido para F71 e S1. A estrutura química de S1 foi definida como sendo o plumierídeo por ressonância magnética nuclear. A avaliação de atividade antiplasmódica foi realizada com EEHS, EEHSP, FrDCM EEHS, FrAcOET EEHS, FrMeOH EEHS, FAHS1 DCM, FAHS2 DCM, FAEEHS, FNEEHS, FNHS DCM e S1 pelo método da lactato desidrogenase parasitária (pLDH). Na prospecção fitoquímica, EEHS e EEHSP apresentaram resultados positivos para polifenóis e taninos, saponinas, triterpenos e esteroides, alcaloides e geninas flavônicas. EEHS e frações analisadas por CLAE-DAD mostraram os picos mais intensos sugestivos de iridoides, comprovados por CL-EM de EEHS que mostrou como substância majoritária o iridóide plumierídeo, coerente com o espectro no IV de F71 e S1 que revelou absorções de grupos funcionais presentes em iridóides. Nas análises destas por CL-EM, observou-se um pico a m/z 471 (M+H), atribuído ao íon pseudomolecular do plumierídeo e/ou isoplumierídeo. S1 foi identificada como plumierídeo. Até o presente momento não foi relatada a presença de alcaloides para a espécie, porém nas frações alcaloídicas (FAHS1 DCM, FAHS2 DCM, FAEEHS) e de neutros (FNEEHS e FNHS DCM) em análise por CCD revelada com reagente Dragendorff observaram-se manchas sugestivas de alcalóides, coerente com análise por CLAE-DAD de FAHS2 DCM, a qual mostrou pico majoritário com cromóforo sugestivo de alcaloide β-carbolínico. Em análises por CL-EM de FAHS2 DCM observou-se pico majoritário sugestivo do alcalóide 10-hidroxi-antirina-N-óxido com massa molecular 328 u. Os ensaios de atividade antiplasmódica foram negativos para EEHS, EEHSP, FrDCM EEHS, FrAcOET EEHS, FrMeOH EEHS, S1, FAEEHS, FAHS1 DCM, FNEEHS, FNHS DCM e moderadamente ativo (CI50 22,89 μg/mL) para FAHS2 DCM. Estes resultados indicam que a atividade antiplasmódica da planta pode se atribuída aos alcaloides, cuja presença em H. articulatus é descrita pela primeira vez. / The present master thesis describes the phytochemical study and the antiplasmodial activity against Plasmodium falciparum (W2) of Himatanthus articulatus (Vahl) Woodson. The ethanol extract (EEHS) obtained by percolation of the bark powder, gave, after concentration, a precipitate (EEHSP) and a pasty residue that was submitted to lyophilization (EEHS). Fractionation of EEHS was carried out by successively re-extraction with DCM, AcOEt and MeOH under reflux, by acid-base partitioning for separation of alkaloids and by chromatography over silica gel column. Furthermore, the bark powder was extracted with 1 N HCl for the separation of alkaloids. TLC prospection of EEHS and EEHSP was carried out while EEHS, FrDCM EEHS, FrAcOET EEHS, FrMeOH EEHS and FAHS2 DCM were also analyzed by HPLC - DAD. Column chromatography of FrAcOET EEHS afforded a substance (S1). EEHS, the major fraction obtained in its chromatographic column (F71), DCM FAHS2 and S1 were analysed by UPLC-PDA-MS/ESI. Spectroscopic analyses of S1 (IR, UV, MS/ESI, 1H and 13CNMR) allowed its identification as plumieride. Antiplasmodial activity against P. falciparum (W2) was evaluated for EEHS, EEHSP, FrDCM EEHS, FrAcOET EEHS, FrMeOH EEHS, FAHS1 DCM, DCM FAHS2, FAEEHS, FNEEHS, FNHS DCM and S1 by the parasite lactate dehydrogenase (pLDH) method. In the TLC prospection, EEHS and EEHSP showed positive results for polyphenols and tannins, saponins, triterpenes and steroids, alkaloids and flavonoids. EEHS fractions were analyzed by HPLC-DAD showing suggestive peaks of iridoids. Analyses by UPLC-PDA-MS/ESI showed that EEHS contains plumieride as the major substance whose psedomolecular peak was observed at m/z 471 (M + H). S1 was identified as plumeride. Until now it was not reported the presence of alkaloids for the species but spots suggestive of alkaloids were observed on TLC with Dragendorff reagent of FAHS1 DCM, DCM FAHS2, FAEEHS, FNEEHS and FNHS DCM were observed. UV spectra registered online by HPLC-DAD for FAHS2 showed that the major peak is suggestive of a β-carboline alkaloid. UPLC-PDA-MS/ESI analysis of FAHS2 DCM showed an intense peak corresondng to a pseudomolecular ion 329 u that could be possibly attributed to the alkaloid 10-hydroxy-antirine-N-oxide (MM 328 u). The antiplasmodial assays were negative for EEHS, EEHSP, FrDCM EEHS, FrAcOET EEHS, FrMeOH EEHS, S1, FAEEHS, FAHS1 DCM, FNEEHS, FNHS DCM and moderately active (CI50 22,89 μg/mL) for FAHS2 DCM. These results indicate that the antiplasmodial activity might be attributed to the plant alkaloids whose presence in H. articulatus is reported here by the firist time.

V. Hojgrová: Alkaloids of Vinca minor L. and their biological activity VIII. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany. Number of pages 69. This diploma thesis deals with the isolation of alkaloids from Vinca minor L. from the family Apocynaceae. Separation of alkaloids from the selected fraction (VM 215-258) or from their subfractions (VM 34-41, VM 86, VM 87-113) was performed by preparative TLC. Two pure alkaloids were isolated from the subfraction (VH 34-41). The first VH-1 alkaloid not yet isolated and the second VH 2 alkaloid: (-)-raucubainin. Alkaloids were identified by EI-MS, LC-MS, NMR and optical rotation and were compared with data in the literature. Isolated alkaloids were tested for acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and prolyloligopeptidase (POP) inhibitory activity and for cytotoxicity. Both substances did not show significant cholinesterase inhibitory activity IC50 against AChE after measurement, only (-)-raucubainin showed a slight activity against BuChE (IC50 = 94 ± 7 μM), VH-1 was found to be inactive (IC50 > 100 μM). POP inhibitory activity has so far only been tested for (-)-raucubainin; was found to be inactive (IC50 > 1000 µM). The results of the cytotoxic activity of the alkaloids...

Submitted by Cleide Dantas (cleidedantas@ufpa.br) on 2014-07-21T13:11:39Z No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_AbordagemFitoquimicaDeterminacao.pdf: 2599690 bytes, checksum: 0968a3faa969681e73e4bfc3c74a9337 (MD5) / Approved for entry into archive by Ana Rosa Silva (arosa@ufpa.br) on 2014-09-08T13:10:10Z (GMT) No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_AbordagemFitoquimicaDeterminacao.pdf: 2599690 bytes, checksum: 0968a3faa969681e73e4bfc3c74a9337 (MD5) / Made available in DSpace on 2014-09-08T13:10:10Z (GMT). No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_AbordagemFitoquimicaDeterminacao.pdf: 2599690 bytes, checksum: 0968a3faa969681e73e4bfc3c74a9337 (MD5) Previous issue date: 2011 / A malária é uma doença causada por protozoários do gênero Plasmodium. O tratamento da malária está se tornando cada vez mais difícil com a expansão dos casos de parasitas resistentes aos fármacos utilizados na terapêutica. Neste contexto, produtos isolados a partir de plantas têm dado importante contribuição, representando importante fonte para a obtenção de novos fármacos antimaláricos. A atividade antiplasmódica de alcalóides de origem vegetal tem sido amplamente relatada na literatura. Plantas da família Apocynaceae, ricas em alcalóides indólicos, apresentam amplas propriedades medicinais e algumas espécies do gênero Aspidosperma já demonstraram potencial antimalárico. Assim, este trabalho teve como objetivo realizar uma abordagem fitoquímica, avaliar a atividade antiplasmódica in vitro e a toxicidade preliminar do extrato hidroetanólico concentrado das cascas de A. excelsum, nativa da Região Amazônica, onde é usada tradicionalmente para tratar várias enfermidades, inclusive malária. A atividade antiplasmódica in vitro de diferentes concentrações do extrato e frações alcaloídica e metanólica foi avaliada em culturas de P. falciparum W2 pela percentagem de inibição da parasitemia e determinação da concentração inibitória média (CI50) em intervalos de 24, 48 e 72 h. O ensaio de citotoxicidade do extrato e fração alcaloídica foi realizado em fibroblastos L929 de camundongo pelo método do MTT e o teste de toxicidade aguda oral do extrato foi realizado de acordo com o Procedimento de Dose Fixa adotado pela OECD com pequenas adaptações. A prospecção fitoquímica revelou a presença de saponinas, açúcares redutores, fenóis e taninos e alcalóides e estes foram confirmados em quantidades significativas na fração alcaloídica extraída com clorofórmio (C2). Através de cromatografia em camada delgada e cromatografia líquida de alta eficiência do extrato, foi caracterizada a presença do alcalóide indólico ioimbina. O extrato e as frações apresentaram atividade antiplasmódica in vitro. O extrato apresentou a melhor atividade em 24 h (CI50= 5,2 ± 4,1 μg/mL), indicando uma boa atividade esquizonticida. Apenas a fração alcaloídica C2 apresentou uma pequena, porém significativa citotoxicidade (concentrações superiores a800 μg/mL). O extrato não só não apresentou citotoxicidade como também nenhum sinal evidente de toxicidade aguda oral na dose de 5000 mg/mL. Os resultados obtidos indicam que o extrato de Aspidosperma excelsum Benth apresenta promissor potencial antimalárico, merecendo estudos mais detalhados sobre sua atividade antiplasmódica, com vistas no isolamento de compostos ativos e elucidação de seu(s) mecanismo(s) de ação. / Malaria is a disease caused by protozoa of the genus Plasmodium. The treatment of malaria is becoming increasingly difficult with the expansion of the cases of parasites resistant to drugs used in therapy. In this context, products isolated from plants have give an important contribution, representing an important source for obtaining new antimalarial drugs. Antiplasmodial activity of alkaloids of plant origin has been widely reported in the literature. Plants of the Apocynaceae family, rich in indole alkaloids have medicinal properties and some large species of the genus Aspidosperma have demonstrated antimalarial potential. Thus, this study aimed to perform a phytochemical approach, evaluate the antiplasmodial activity and toxicity in vitro preliminary of the hydroethanolic extract from the bark of A. excelsum, native of the Amazon region, where it is traditionally used to treat various diseases, including malaria. Antiplasmodial activity in vitro of different concentrations of the extract and alkaloidal and methanolic fractions was evaluated in cultures of P. falciparum W2 by the percentage of inhibition of parasitaemia and the mean inhibitory concentration (IC50) was determined at intervals of 24, 48 and 72 h. The cytotoxicity assay of the extract and alkaloidal fraction was carried out on L929 mouse fibroblasts by MTT method and the testing of acute oral toxicity of the extract was carried out according to the Fixed Dose Procedure adopted by the OECD with small modifications. The phytochemical approach revealed the presence of saponins, reducing sugars, phenols and tannins and alkaloids, and these were confirmed in significant amounts in the alkaloidal fraction with chloroform fraction (C2). Through thin layer chromatography and high performance liquid chromatography of the extract characterized the presence of the indole alkaloid yohimbine. The extract and fractions showed antiplasmodial activity in vitro. The extract showed the best activity in 24 h (IC50 = 5.2 ± 4.1 μg / mL), indicating a good activity schizonticide. Only C2 alkaloidal fraction showed a small but significant cytotoxicity (concentrations higher than 800 μg/mL). The extract not only cytotoxicity but also did not showed any obvious sign of toxicity in acute oral dose of 5000 mg/mL. The results indicate that the extract of Aspidosperma excelsum Benth presents promising potential antimalarial and deserves more detailed studies on antiplasmodial activity, aiming the isolation of active compounds and elucidation of their mechanisms of action.

This thesis deals with phylogenetic relationships at different taxonomic levels. All the plants studied are or have been included in the order Gentianales, which comprises about 17 200 species. The phylogenies are based on parsimony analyses of nucleotide sequence data (DNA) from different regions in the chloroplast genome, but morphological characteristics have also been studied. Analyses of sequence data from the genes rbcL and ndhF provide the interfamilial structure of the order Gentianales, shown to comprise the families Apocynaceae (incl. Asclepiadaceae), Gelsemiaceae, Gentianaceae, Loganiaceae, and Rubiaceae. Exclusion of certain genera from the Loganiaceae is confirmed and their phylogenetic positions are clarified. Some of these genera remain within the Gentianales, while others belong to other orders. Exclusion of the tribe Buddlejeae from the Loganiaceae is confirmed, and a monophyletic group formed by Buddleja, Emorya, Gomphostigma, and Nicodemia is recognized and placed in the Lamiales. The Loganiaceae s.str. forms, after these exclusions, a strongly supported monophyletic group comprising 13 genera. The tribe Paederieae in the family Rubiaceae is analysed using sequence data from the regions rbcL gene, rps16 intron, and the regions trnT-F, and is shown to be paraphyletic. These results lead to a new circumscription of Paederieae comprising the genera Leptodermis, Paederia, Serissa, and Spermadictyon. The tribe Putorieae is reestablished with the single genus Plocama, including 34 species. Aitchisonia, Choulettia, Crocyllis, Gaillonia, Jaubertia, Pseudogaillonia, Pterogaillonia, and Putoria are reduced to synonyms of Plocama based on the molecular analyses and morphological studies. The Mediterranean species of the re-circumscribed Plocama, previously segregated as the genus Putoria, are revised. Two species, Plocama calabrica and P. brevifolia, are recognized, their synonymics are established, and seven lectotypes are selected.

MSc (Chemistry) / Department of Chemistry / Rauvolfia caffra Sond, a species of evergreen trees and shrubs in the dogbane family, (Apocynaceae), is used as a medicinal plant among traditional communities in many countries for the treatment of malaria, diabetes, coughs, gastrointestinal disturbances, skin infections, impotence, insomnia, diarrhoea, dysentery, scabies, worm infections, and both parasitic and microbial infections. Phytochemical studies have revealed that indole alkaloids are the major constituents of the stem bark. However, there are limited studies linking the compounds with the ethnomedicinal uses. The aim of this study is to isolate and characterize bioactive compounds from Rauvolfia caffra Sond. The highest phenolic content found in a fraction was 16.06±0.125 mg GAE/g, while the highest flavonoid content measured was 9.453±0.081 mg QE/g. In the DPPH free radical scavenging activity and reducing power tests, a lowest IC50 value of 0.022±0.003 μg/mL and IC0.5 value 0.518±0.044 μg/mL, respectively, was found. Six compounds were isolated from the stem bark, including lupeol, a pentacyclic tri-terpenoid isolated for the first time from the genus Rauvolfia; raucaffricine, a rare glycoalkaloid of the monoterpenoid indole class; N-methylsarpagine, an indole alkaloid isolated for the time from R. caffra and spegatrine, an indole alkaloid isolated for the first time from R. caffra, respectively. Concerning antimicrobial activity, the highest activity of a fraction was against B. cereus with MIC values as low as 12.5 mg/mL. One fraction at the tested concentration (250 μg/mL) decreased the viability of Plasmodium falciparum (4.149±6.979 %) with an IC50 value of 6.533 μg/mL. The crude extract and some fractions affected the viability of the Trypanosomes at the tested concentration (250 μg/mL), giving -0.133 ± 0.206 %, 11.334 ± 2.692 %, 1.026 ± 0.143 % and 20.769 ± 9.054 % with IC50 values of 18.50 μg/mL, 14.15 μg/mL, 15.58 μg/mL and 34.71 μg/mL, respectively. Furthermore, the fractions did not show significant cytotoxic effects at a concentration of 50 μg/mL. / NRF