Watershed systems are experiencing rapid changes to water quality and hydrologic regimes due in part to climate-induced changes in temperature and precipitation, urbanization, and increases in aquatic invasive species. Aquatic invasive species are one of the primary threats to ecosystems, contributing to loss of biodiversity, altered hydrologic regimes, and stream degradation. Urban land use and climatic factors influence the spread of invasive species, presenting greater challenges for future invasive species management. There is a need for more research that evaluates the watershed process in connection with urban land use and climate change factors in relation to invasive species spread. This study will examine factors of climate change and land use that may be influencing the spread and occurrence of aquatic invasive plants within the Connecticut River watershed. There will be four species involved in this study: Eurasian milfoil (Myriophyllum spicatum), Variable milfoil (Myriophyllum heterophyllum), Hydrilla (Hydrilla verticillate), and the European water chestnut (Trapa natans). Hydrological conditions within the watershed will be analyzed using the SWAT model through the HAWQS interface. ArcGIS Pro will be used to combine and prepare data so that it may be utilized through MaxEnt. MaxEnt will be used to create species distribution models to estimate the probability of the presence of invasive aquatic plant species in the Connecticut river watershed.

Dissertation submitted in partial compliance with the requirements for the Masters Degree in Technology, 2010. / Water storage dams play an important part in the collection and purification of water destined for human consumption. However, the nutrient rich silt in these dams promotes rapid growth of aquatic plants which tend to block out light and air. Glyphosate is universally used as the effective non-selective herbicide for the control of aquatic plants in rivers and dams. Invariably there is residual glyphosate present in water after spraying of dams and rivers with glyphosate herbicide. The amount of residual glyphosate is difficult to determine on account of high solubility of glyphosate in water. Thus a method of sample preparation and a sensitive HPLC method for the detection of trace amounts of glyphosate and its major metabolite aminomethylphosphonic acid (AMPA) in water is required. A crucial step in sample preparation is pre-column derivitization of glyphosate with 9-fluorenylmethyl chloroformate (FMOC-Cl). For sample pretreatment, water samples were derivatized with FMOC-Cl at pH 9, extracted with ethyl acetate and sample clean-up was carried out by passing a sample through the SPE cartridge. For SPE, recovery studies were done to choose a suitable cartridge for glyphosate and AMPA analysis. The following cartridges were compared, namely, C18, Oasis HLB and Oasis MAX SPE cartridges. Best recoveries (101% for glyphosate and 90% for AMPA) were obtained using 500 mg of C18 solid-phase extraction cartridge. The eluent from SPE cartridge was injected into HPLC column. Three types of separation columns (namely; C18 column, silica based amino column and polymeric amino column) were compared for the separation of glyphosate and AMPA. The best separation of glyphosate and AMPA in water samples was achieved using a polymeric amino column and a mobile phase at pH 10 which contained a mixture of acetonitrile and 0.05 M phosphate buffer (pH 10) 55:45, (v/v) respectively. The method was validated by spiking tap water , deionized water and river water at a level of 100 μg/l. Recoveries were in the range of 77% -111% for both analytes. The method was also used in determining the levels of glyphosate and AMPA in environmental samples. This method gave detection limits of 3.2 μg/l and 0.23 μg/l for glyphosate and AMPA respectively. The limits of quantification obtained for this method were 10.5 μg/l and 3.2 μg/l for glyphosate and AMPA respectively. / Eskom Tertiary Education Support Programme (TESP) Durban University of Technology.

The extent and quality of freshwater systems is declining globally. Combined with past drainage, straightening and flow regulation, current systems are often functional but not pristine. Conservation, creation and restoration of freshwater systems is common but requires significant planning, resources and active monitoring and may only be a short-term solution to the long-term problem of destruction and loss of riparian zones. Beavers (Castor spp.) have the ability to create physical and biological habitat heterogeneity through the construction of woody debris dams, thereby restoring lost natural discontinuities in freshwater systems. Beavers may thus offer a natural, more passive solution to the need for wetland restoration or creation and the problem of homogenisation of watercourses. As such, numerous beaver reintroductions and introductions have been undertaken based in part on restoring this lost natural heritage. However, it is crucial to be able to predict the potential effects on existing biota of physical modifications by beavers to ecosystems, especially in the light of further population expansion, whilst also disentangling these effects from other influences, namely herbivory. The impact of beavers on aquatic systems was studied using a combination of field-based surveys and experiments, using aquatic plants and macro-invertebrates as indicators of hydromorphological changes and to quantify the effects of direct foraging. The research presented in this thesis demonstrates beaver adaptive foraging behaviours between terrestrial and aquatic habitats, whilst feeding highly selectively, optimally and opportunistically, using the white water lily (Nymphaea alba) as a model species. The effects of beaver foraging on the aquatic plant resource and diversity was low over short time spans (e.g. 1 year), but when selective foraging was assessed over greater time scales (e.g. 10 years) the effects of foraging were distinct. Significant changes in aquatic plant height, biomass, richness, diversity and composition were observed over this time period due to selective grazing on large rhizomatous species (e.g. Menyanthes trifoliata). These direct effects occurred even though changes in water levels, which are commonly believed to be the main driver of beaver influence on aquatic vegetation, were negligible. In a separate study in Sweden where beavers commonly constructed dams, with ponds then forming upstream, the aquatic plant and coleoptera species richness and composition differed in comparison to adjacent non-beaver created wetlands. Therefore, having a range of wetland types in the environment increases physical and biological heterogeneity creating unique niches that are exploited by disparate taxa. The construction of a series of dams within a single reach of stream flowing through a Scottish agricultural landscape also increased physical habitat diversity. Distinctive macroinvertebrate assemblages and modified functional diversity were associated with each dominant habitat type in the stream, resulting in increased landscape scale richness. The findings of this thesis confirm that beaver engineering and foraging has the potential to create unique and highly heterogeneous wetland and stream habitats within landscapes that enhances richness and diversity for multiple species groups. This thesis also supports part of the rationale for the trial reintroduction of beaver to Scotland that beavers can restore degraded habitats.

This thesis looks into the use of historical macrophyte records to assess long term changes in macrophyte communities in lakes and potential reasons for these changes. In particular it uses historical records to assess changes in macrophyte communities in the Norfolk Broads and West Midland Meres, two sets of lowland, eutrophic lakes in England. It provides a critical examination of the use of historical records, highlighting some of the constraints common to such data such as variations in recording effort, and bias in species recording and site selection. Having acknowledged these issues we then go on to develop a robust way to interpret such data, using a “change index” based on species persistence over the last 200 years within individual lakes. Species with high change index values, which represented species which had persisted or increased within the lake districts, were those known to be characteristic of eutrophic lakes. Conversely species with low index scores, which had declined in both the broads and meres over the last 200 years, included species associated with less fertile conditions but also a selection of typically eutrophic species. Averaging of change index scores in present day survey data served to identify the historically least changed lakes and to rank lakes in order of degree of botanical change over the last century. We then analysed the ecological basis of the change index in order to better understand the processes behind the decline of some species and survival of others in the Norfolk Broads and West Midland Meres. Functional groups determined from morphological and regenerative traits displayed significant differences in change index values in both groups of lakes, but declining taxa occurred across a wide range of plant growth forms. Non-hierarchical clustering of species based on their ecological preferences, obtained from published literature, resulted in groups with distinct change index values, indicating that changes in the status of species could be partly explained by these preferences. Of these, trophic preference was consistently the most important, with species of less fertile habitats consistently experiencing the greatest declines. However, some characteristically eutrophic species have also declined significantly, particularly in the broads. In these cases increasing loss of shallow water, low energy habitats in the broads, or loss of fluctuating water levels and less alkaline backwaters in the meres, appear to have been contributory factors. In addition to the change index approach, we also used historical records at a site level to complement palaeolimnological analysis and investigate the change in macrophyte community composition and structure at Barton Broad, Norfolk. Sediment samples were extracted from the bottom of the broad and analysed for sub-fossil remains and pollen of macrophytes. The historical records and palaeolimnological analysis combined showed that early communities did not consist entirely of low growing, oligotrophic and mesotrophic species as previously thought, but in fact comprised a mixture of these and other more characteristically high nutrient species associated with taller, or free-floating growth habit. As eutrophication progressed throughout the last century, the community was increasingly dominated by these latter growth forms. Diversity was maintained, however, since encroaching reedswamp generated a mosaic of low energy habitats which supported a range of species unable to withstand the hydraulic forces associated with more open water habitat. When the reedswamp disappeared in the 1950s, many of the dependent aquatic macrophytes also declined resulting in widespread macrophyte loss. The thesis demonstrates not just the complexities of using historical records, but also ways in which these can be overcome to make useful observations about macrophyte community change and lake ecological integrity to inform conservation and lake management, both on a site and lake district level.

Hygrophila polysperma is a plant native to Asia that has been introduced into the Comal River, TX and is thriving while Ludwigia repens, a species native to the river appears to be declining. Both plants have similar morphologies and occupy similar habitats in the river. Two plant competition experiments were conducted to examine the competitive interactions between the two species. First, an experimental design was developed in which established Ludwigia plants were 'invaded' by sprigs of Hygrophila to determine if established Ludwigia populations would be negatively impacted by invasion. The second experiment focused on establishment and growth of sprigs of each species under three competition scenarios. Results show that the continued growth of well-established Ludwigia plants was significantly depressed by the invasion of Hygrophila in comparison with those that had not been invaded. Furthermore, the growth of Hygrophila sprigs was uninhibited by the presence of Ludwigia, but the presence of Hygrophila negatively impacted the growth of Ludwigia sprigs. There was no difference in the growth of Hygrophila sprigs whether planted alone, with Ludwigia sprigs or even if planted into stands of established Ludwigia.

Master of Science / Department of Horticulture, Forestry & Recreation Resources / Greg Davis / Urban environmental pollution continues to be exacerbated by a number of factors relating to human population growth including sewage discharged directly into the urban rivers designed with concrete-sealed riverfronts. This has left a number of rivers with deteriorated water quality. Where a riverfront could be the highlight and magnet of the city, it may instead become a stain and waste place. In 1969 American landscape planner McHarg proposed the landscape planning theory, “Design with Nature." His primary argument was that natural processes provide self-regulatory functions that need to be reflected in our plans and designs. Ecological design aims include restoring or promoting natural processes and automatic (bio-physical, regenerative, and adaptive) stabilizers. A wide range of scientific knowledge is available to help guide the designer, but designers usually have limited time to complete their designs. Unfortunately, much of this information is diffusely dispersed in research literature and not easily collected and synthesized by the design community. The purpose of this review is to help provide a synthesis of current thought and to help establish the basis for principles that can aid the designer, offering easy-to-understand design guidelines related to the use of aquatic plants in ecological redevelopment along urban riverfronts. This report focuses on using aquatic plants as the main material to help solve two key problems along riverfront developments: water pollution and flooding. As such this report can serve as a guide for the designer helping them to select aquatic plants using an ecological design approach for the redevelopment of urban riverfronts. It also addresses the essential need to adapt designs based on local site problems and requirements. Since this report provides a review and a basis for where to start in designing with aquatic plants in ecological redevelopment of urban waterfronts, it should not be considered as an exclusive source for the designer but rather a complement to local guidelines and information to derive design solutions.

Thesis (MTech (Horticultural Sciences)--Cape Peninsula University of Technology, 2012 / This study evaluated the effects of different combinations of Hoagland’s solution and Azolla filiculoides on Beta vulgaris subsp. cycla ‘FORDHOOK GIANT’ grown in different hydroponic nutrient solutions. These solutions were comprised of a full Hoagland’s solution and a Hoagland’s solution minus nitrogen solution and amalgamations of these with Azolla respectively. The objectives were to assess the effects of different combinations of Hoagland’s solution and A. filiculoides on uptake of nitrogen and other nutrients, photosynthesis, chlorophyll content, growth and development in B. vulgaris grown in hydroponic cultures. The treatments were made up of 1) Hoagland’s minus N solution (as the control), 2) A. filiculoides plus Hoagland’s minus N solution, 3) A. filiculoides plus a full Hoagland’s solution and 4) full Hoagland’s solution. Each treatment was replicated 4 times. Nutrient uptake was measured at 4 and 8 weeks into the experiment. Photosynthesis was measured by analysing the photosynthetic rate, stomatal conductance, intercellular CO2 concentration and the evapotranspiration rate of B. vulgaris on a weekly basis. Chlorophyll content was determined by analysing the samples at 4 and 8 weeks. Growth and development was determined by measuring plant height, leaf number, leaf colour, fresh weight and dry weight. Plant height, leaf number and leaf colour on a biweekly intervals, while fresh and dry weight were analysed at 4 and 8 weeks into the experiment. In this study, the most favourable results were attained by the full Hoagland’s solution. This treatment produced plants with the highest nutrient uptake, photosynthesis, chlorophyll content and best growth and development. Preceding this was the Azolla plus full Hoagland’s solution, followed by the Azolla plus Hoagland’s minus nitrogen solution. The poorest results were noted in the control (Hoagland’s minus nitrogen solution) as all the tested parameters in this treatment were the lowest. In this study, Azolla plus Hoagland's minus N solution treatment produced significant growth in B. vulgaris. Although nitrogen was not applied in this treatment, there was improved nitrogen content in B. vulgaris organs. It is postulated that, probably, Azolla released the fixed nitrogen in its surrounding environment making it available to B. vulgaris hence improving N uptake and growth. This implies that there was a synergistic effect from Azolla–Anabaena symbiosis. More studies to understand the mechanisms involved in improving the plant growth are recommended.

<p>The aquatic plant <i>Ipomoea aquatica</i> is a popular vegetable in Southeast Asia, often cultivated in nutrient rich and polluted waters. The overall aim of this thesis was to estimate potential risks for human health and reduced plant growth due to accumulation and toxicity of total-Hg, methyl-Hg, Cd and Pb.</p><p>In plants from cultivations in Thailand, the concentrations of Cd and Pb in the shoots were well beneath recommended maximum values for human consumption, but at some sites the Hg concentrations were high. It was demonstrated that <i>I. aquatica</i> has the capacity to accumulate much higher Cd and Pb concentrations in the shoots than found in field-cultivations, before exhibiting toxic symptoms. The Hg concentrations, however, occasionally reached levels that are toxic for the plant. Up to11% of total-Hg was methyl-Hg, the most toxic Hg species, though at one site it was 50-100%. To study if methyl-Hg is formed in <i>I.</i> <i>aquatica</i>, plants were exposed to inorganic Hg through the roots. Of the Hg that reached the young, metabolically active parts of the shoots, a part was transformed to methyl-Hg. A major proportion of absorbed metals was retained in the roots, which had a high tolerance for high internal metal concentrations. </p><p>The nutrient level did influence accumulation and effects of Hg, Cd and Pb in<i> I. aquatica</i>. Low external nutrient levels resulted in increased metal accumulation in the shoots and in metal-induced toxic effects in the plant at low external metal levels. A generous supply of sulphur or nitrogen induced formation of thiol-rich peptides in <i>I. aquatica</i>, compounds that have a metal detoxifying effect in plants. </p><p>To conclude, the levels of Cd and Pb in field cultivated <i>I. aquatica</i> do not pose any apparent threat to human health or risk for reduced plant growth. The levels of Hg however, were high at some sites and could be a health threat, for children and foetuses in particular, and especially considering the presence of methyl-Hg. The use of fertilizers is favourable as it reduces the risk for increased metal concentrations in <i>I.</i> <i>aquatica</i> and for reduced crop yields. </p>

The aquatic plant Ipomoea aquatica is a popular vegetable in Southeast Asia, often cultivated in nutrient rich and polluted waters. The overall aim of this thesis was to estimate potential risks for human health and reduced plant growth due to accumulation and toxicity of total-Hg, methyl-Hg, Cd and Pb. In plants from cultivations in Thailand, the concentrations of Cd and Pb in the shoots were well beneath recommended maximum values for human consumption, but at some sites the Hg concentrations were high. It was demonstrated that I. aquatica has the capacity to accumulate much higher Cd and Pb concentrations in the shoots than found in field-cultivations, before exhibiting toxic symptoms. The Hg concentrations, however, occasionally reached levels that are toxic for the plant. Up to11% of total-Hg was methyl-Hg, the most toxic Hg species, though at one site it was 50-100%. To study if methyl-Hg is formed in I. aquatica, plants were exposed to inorganic Hg through the roots. Of the Hg that reached the young, metabolically active parts of the shoots, a part was transformed to methyl-Hg. A major proportion of absorbed metals was retained in the roots, which had a high tolerance for high internal metal concentrations. The nutrient level did influence accumulation and effects of Hg, Cd and Pb in I. aquatica. Low external nutrient levels resulted in increased metal accumulation in the shoots and in metal-induced toxic effects in the plant at low external metal levels. A generous supply of sulphur or nitrogen induced formation of thiol-rich peptides in I. aquatica, compounds that have a metal detoxifying effect in plants. To conclude, the levels of Cd and Pb in field cultivated I. aquatica do not pose any apparent threat to human health or risk for reduced plant growth. The levels of Hg however, were high at some sites and could be a health threat, for children and foetuses in particular, and especially considering the presence of methyl-Hg. The use of fertilizers is favourable as it reduces the risk for increased metal concentrations in I. aquatica and for reduced crop yields.

Rund 3.000 Arten zur Beschreibung des ökologischen Zustandes von Gewässern nach den Vorgaben der Europäischen Wasserrahmenrichtlinie wurden bisher in Sachsen erfasst. Die größte Gruppe stellen Wasserpflanzen einschließlich Algen dar. Die reich bebilderte Broschüre stellt eine Auswahl in Sachsen vorkommender Wasserpflanzen und am Gewässerboden lebender Algen vor und gibt Einblick in eine faszinierende Unterwasserwelt. Sie ermöglicht wasserwirtschaftlichen Praktikern und interessierten Naturschützern einen Einstieg in die Interpretation des ökologischen Gewässerzustands.