Field pea (Pisum sativum L.) is highly susceptible to ascochyta blight, primarily caused by the pathogen Mycosphaerella pinodes (Berk. & Blox.) Vestergr. Grasspea (Lathyrus sativus L.) has been reported to possess a moderate level of resistance to ascochyta blight caused by M. pinodes. The work reported in this thesis aimed to develop the various techniques that would be required to transfer ascochyta blight resistance from grasspea into field pea via somatic hybridisation. This thesis also assesses the feasibility of achieving this goal. Field pea shoot cultures were established on hormone-free MS medium, and a protoplast isolation protocol developed for both species. Grasspea shoot cultures were established on both RL and SSB8 medium. Friable grasspea callus was achieved on media supplemented with 2,4-D in the range 4.523 µM, whereas kinetin tested at any concentration did not appear to influence callus growth. A suspension culture of grasspea was developed for the first time, in B5 medium supplemented with 4.5 µM 2,4-D and 0.5 µM kinetin. Grasspea protoplasts were isolated from both in vitro seedlings and suspension cultures. Protocols for hybrid shoot culture on KM8p medium were developed via organogenesis and somatic embryogenesis.

Bibliography: leaves 182-217. This study was conducted to determine the etiology of a blight disease of chickpea in south-eastern Australia and the factors affecting disease development. The disease had previously been identified as phoma blight. Pathogenicity testing revealed two isolates subsequently identified as Asochyta rabiei, the first conclusive identification in the southern hemisphere. Greenhouse screening of chickpea varieties identified types resistant to ascochyta blight. The effects of plant age and environmental conditions on disease development were investigated under controlled conditions in growth rooms. Seedlings were more susceptible than older plants. The optimum conditions for ascochyta blight were 20° C and a 48-96 h period of leaf wetness. Through field trials it was found that disease intensity increased over time, especially in cv. Desavic. The means of penetration of the chickpea host was established in histological studies. This study provided advance warning of this disease for the expanding chickpea industry, and has allowed the implementation of appropriate disease management strategies. It is recommended that cv. Desavic should not be grown where ascochyta blight is likely to be a problem.

Successful chickpea production in western Canada typically requires multiple applications of fungicides to minimize the severity of Ascochyta blight (AB) caused by <i>Ascochyta rabiei</i>. Although planting resistant cultivars could be economical and environmentally safer than fungicide usage, varieties with a high level of resistance are not available. The objectives of this research were i) to determine the effect of different seeding arrangement treatments on ascochyta blight severity and seed yield of two cultivars (moderately resistant and susceptible) of kabuli chickpea; ii) to compare one and four fungicide applications at recommended and reduced rates and their impact on disease severity and cost; and iii) to assess organ-specific reaction to AB in chickpea in leaves, stems and pods of 12 desi and 12 kabuli varieties that are of economic significance to western Canada. <p>Treatments significantly influenced AB severity on both moderately resistant and susceptible cultivars in a season with a severe epidemic. Seed yield was significantly influenced by treatments for both varieties in both years. Contrast analyses revealed that four fungicide applications significantly reduced the AB severity for both varieties in a season with a severe epidemic and for the susceptible variety in a season with a moderate epidemic. Seed yield of both varieties was significantly higher under four fungicide applications compared to a single application. Solid seeding and paired row arrangements did not differ in their effect on seed yield and AB severity for both varieties in both years, except that the susceptible variety benefited from paired row planting with respect to seed yield and reduced AB severity in the season with a severe epidemic. Reducing fungicide rates and seeding rate could reduce the cost of cultivation without significantly affecting disease control and yield. Economic assessment revealed that in a severe epidemic season, the gross returns were high for the moderately resistant variety under four fungicide applications than one fungicide application. Gross returns for the susceptible variety were higher under four fungicide applications in both years.<p>There were differences among varieties for AB severity on leaves, stems and pods, seed yield and 1000 seed weight at all site-years tested. The variation was greater in kabuli varieties than desi varieties. AB severity on leaves, stems and pods was lower under high fungicide regimes, with few exceptions. Varieties with a fern leaf type had lower AB severity than those with unifoliate leaves. There was a positive correlation among AB severity on leaves, stems and pods. No differences in organ-specific reaction were observed.

Random amplified polymorphic DNA (RAPD) markers were used in three genetic studies in lentil (<i>Lens culinaris</i> Medikus). The first study involved development of RAPD markers linked with genes for resistance to ascochyta blight, caused by <i>Ascochyta fabae</i> f. sp. lentis Gossen et al. Seventy F₂-derived F₃ (F_2:3) lines were field screened for ascochyta blight reaction in each of two hybrid populations, Indianhead x Eston and ILL 5588 x Eston, that were segregating for resistance to ascochyta blight. Resistance to ascochyta blight in ILL 5588 lentil was conferred by a single dominant gene (<i>Ral₁</i>), whereas resistance in Indianhead lentil was conferred by a single recessive gene (<i>ral₂</i>). An efficient DNA extraction procedure and a PCR protocol that yielded RAPD markers with high resolution were developed for lentil. Bulked segregant analysis was used to produce four bulks of DNA from resistant vs. susceptible F₂ plants in each of these two populations which were then screened for RAPD markers using 400 random oligonucleotide primers. One RAPD marker, UBC227₁₂₉₀, was linked to the recessive gene, <i>ral₂</i>, in Indianhead lentil in repulsion phase with a map distance of 14.1 ± 4.5 cM. No RAPD marker was linked with the <i>Ral₁</i> gene in ILL 5588 lentil. In the second study, RAPD markers were used to study phylogeny of the genus Lens. DNA, extracted from 23 accessions of all five taxa of the genus Lens (culinaris, orientalis, nigricans, odemensis and ervoides), was screened for RAPD polymorphisms, using 11 random oligonucleotide primers. Two accessions of the differentiated cytotype of L. nigricans were also included. One hundred and forty eight polymorphic RAPD markers were resolved. A dendrogram for these RAPD markers, using the unweighted pair group method, clearly separated all accessions into their supposedly related taxa. Lens orientalis was the undisputed progenitor of the cultivated lentil, <i>Lens culinaris</i>. A low level of RAPD polymorphism was observed in <i>Lens culinaris</i> and L. ervoides. The differentiated cytotype of L. nigricans was well separated from the normal cytotype of L. nigricans and was closely associated with the L. odemensis accessions, indicating its close genetic similarity to L. odemensis. Principal component analysis (PCA) also indicated a similar relationship among these accessions, but resulted in a better resolution of the groupings. In the third study, the genetic polymorphism of seven Canadian lentil cultivars were studied using RAPD markers. Four lentil cultivars, CDC Gold, CDC Matador, Eston and Indianhead each had a unique, cultivar-specific RAPD marker, allowing their identification.

Successful chickpea production in western Canada typically requires multiple applications of fungicides to minimize the severity of Ascochyta blight (AB) caused by <i>Ascochyta rabiei</i>. Although planting resistant cultivars could be economical and environmentally safer than fungicide usage, varieties with a high level of resistance are not available. The objectives of this research were i) to determine the effect of different seeding arrangement treatments on ascochyta blight severity and seed yield of two cultivars (moderately resistant and susceptible) of kabuli chickpea; ii) to compare one and four fungicide applications at recommended and reduced rates and their impact on disease severity and cost; and iii) to assess organ-specific reaction to AB in chickpea in leaves, stems and pods of 12 desi and 12 kabuli varieties that are of economic significance to western Canada. <p>Treatments significantly influenced AB severity on both moderately resistant and susceptible cultivars in a season with a severe epidemic. Seed yield was significantly influenced by treatments for both varieties in both years. Contrast analyses revealed that four fungicide applications significantly reduced the AB severity for both varieties in a season with a severe epidemic and for the susceptible variety in a season with a moderate epidemic. Seed yield of both varieties was significantly higher under four fungicide applications compared to a single application. Solid seeding and paired row arrangements did not differ in their effect on seed yield and AB severity for both varieties in both years, except that the susceptible variety benefited from paired row planting with respect to seed yield and reduced AB severity in the season with a severe epidemic. Reducing fungicide rates and seeding rate could reduce the cost of cultivation without significantly affecting disease control and yield. Economic assessment revealed that in a severe epidemic season, the gross returns were high for the moderately resistant variety under four fungicide applications than one fungicide application. Gross returns for the susceptible variety were higher under four fungicide applications in both years.<p>There were differences among varieties for AB severity on leaves, stems and pods, seed yield and 1000 seed weight at all site-years tested. The variation was greater in kabuli varieties than desi varieties. AB severity on leaves, stems and pods was lower under high fungicide regimes, with few exceptions. Varieties with a fern leaf type had lower AB severity than those with unifoliate leaves. There was a positive correlation among AB severity on leaves, stems and pods. No differences in organ-specific reaction were observed.

No description available.

Bibliography: leaves 182-217. / xx, 217, [18] leaves, [17] leaves of plates : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This study was conducted to determine the etiology of a blight disease of chickpea in south-eastern Australia and the factors affecting disease development. The disease had previously been identified as phoma blight. Pathogenicity testing revealed two isolates subsequently identified as Asochyta rabiei, the first conclusive identification in the southern hemisphere. Greenhouse screening of chickpea varieties identified types resistant to ascochyta blight. The effects of plant age and environmental conditions on disease development were investigated under controlled conditions in growth rooms. Seedlings were more susceptible than older plants. The optimum conditions for ascochyta blight were 20C and a 48-96 h period of leaf wetness. Through field trials it was found that disease intensity increased over time, especially in cv. Desavic. The means of penetration of the chickpea host was established in histological studies. This study provided advance warning of this disease for the expanding chickpea industry, and has allowed the implementation of appropriate disease management strategies. It is recommended that cv. Desavic should not be grown where ascochyta blight is likely to be a problem. / Thesis (Ph.D.)--University of Adelaide, Dept. of Applied and Molecular Ecology, 1999

Thesis (Ph.D.)--University of Western Australia, 2008.

No description available.

[Truncated abstract] The genetics of ascochyta blight resistance was studied in five 5 x 5 half-diallel cross sets involving seven genotypes of chickpea (ICC 3996, Almaz, Lasseter, Kaniva, 24B-Isoline, IG 9337 and Kimberley Large), three accessions of Cicer reticulatum (ILWC 118, ILWC 139 and ILWC 184) and one accession of C. echinospermum (ILWC 181) under field conditions. Both F1 and F2 generations were used in the diallel analysis. Almaz, ICC 3996 and ILWC 118 were the most resistant genotypes. Estimates of genetic parameters, following Hayman's method, showed significant additive and dominant gene actions. The analysis also revealed the involvement of both major and minor genes. Susceptibility was dominant over resistance to ascochyta blight. The recessive alleles were concentrated in the two resistant chickpea parents ICC 3996 and Almaz, and one C. reticulatum genotype ILWC 118. High narrow-sense heritability (ranging from 82 to 86% for F1 generations, and 43 to 63% for F2 generations) indicates that additive gene effects were more important than non-additive gene effects in the inheritance of the trait and greater genetic gain by breeding resistant chickpea cultivars using carefully selected parental genotypes. Current simple leaf varieties are often susceptible to ascochyta blight disease whereas varieties of other leaf types range from resistant to susceptible. The inheritance of ascochyta blight resistance and different leaf types and their correlation were investigated in intraspecific progeny derived from crosses among two resistant genotypes with normal leaf type (ICC 3996 and Almaz), one susceptible simple leaf type (Kimberley Large) and one susceptible multipinnate leaf type (24 B-Isoline). ... An interspecific F2 mapping population derived from a cross between chickpea accession ICC 3996 (resistant to ascochyta blight, early flowering, and semi-erect plant growth habit) and C. reticulatum accession ILWC 184 (susceptible to ascochyta blight, ii late flowering, and prostrate plant growth habit) was used for constructing a genetic linkage map. F2 plants were cloned through stem cuttings taken at pre-flowering stage, treated with plant growth regulator powder (0.5 mg/g indole butyric acid (IBA) and 0.5 mg/g naphthalene acetic acid (NAA)) and grown in a sand + potting mix substrate. Clones were screened for ascochyta blight resistance in controlled environment conditions using a 19 scale. Three quantitative trait loci (QTLs) were found for ascochyta blight resistance in this population. Two linked QTLs, located on linkage group (LG) 4, explained 21.1% and 4.9% of the phenotypic variation. The other QTL, located on LG3, explained 22.7% of the phenotypic variation for ascochyta blight resistance. These QTLs explained almost 49% of the variation for ascochyta blight resistance. LG3 had two major QTLs for days to flowering (explaining 90.2% of phenotypic variation) and a major single QTL for plant growth habit (explaining 95.2% of phenotypic variation). There was a negative correlation between ascochyta blight resistance and days to flowering, and a positive correlation between days to flowering and plant growth habit. The flanking markers for ascochyta blight resistance or other morphological characters can be used in marker-assisted selections to facilitate breeding programs.