MSCAGR / Department of Plant Production / Chickpea (Cicer aritienum L.) is an ancient crop that originated in South-Eastern Turkey and belongs to the genus Cicer, tribe Cicereae, and family Fabaceae. Chickpea has the ability to fix atmospheric nitrogen (N) for its growth. However, chickpea productivity not only depends on N2 fixation or dry matter accumulation, but also the effectiveness of nutrient partitioning to seed, a key component to overall yield. There is a dearth of information on the effect of P with rhizobial inoculation in response of nutrients accumulation in the rhizosphere, shoots and grain of chickpea, especially when determined at different growth stages in the African continent. This study contributes knowledge on this crucial aspect which will likely lead to more other similar research reports in other settings. Therefore, the objectives of this study was to evaluate the effect of P fertilizer rates and rhizobial inoculation on yield and nutrients accumulation in two chickpea genotypes. Field experiments were conducted in winter 2017 and 2018 at University of Venda, Thohoyandou and University of Limpopo’s experiment farm, Syferkuil. Treatments consisted of a factorial combination of two rates of P fertilizer (0 and 90 kg P ha-1), two desi chickpea genotypes (ACC1 and ACC5) and two rhizobial inoculation levels (with and without rhizobiam strain). The treatments were laid out in a randomized complete block design (RCBD) and replicated three times on 22 April 2017 and 11 April 2018 (Syferkuil), 13 April 2017 and 29 April 2018 (Thohoyandou). Macronutrients including P, K, Ca, Mg were determined using the citric acid method. The total N concentration were determined by the micro-Kjeldahl method in both soil, shoots and grain. Zn was extracted using a di-ammonium ethylenediaminetetraacetic acid (EDTA) solution. The content of macronutrients (P, K, Ca, Mg, Ca, and Zn) in soil, shoots and grain was determined by first subjected to wet digestion (Mehlich, 1984). From the digest, various elements were read using relevant procedures. P contents was determined colorimetrically using a spectrophotometer. Yield and yield components were assessed at harvest maturity. Genotypes affected the accumulation of mineral elements in rhizosphere soil, shoots, grain and yield. Accession 5 performed better in most of nutrients elements compared to accession 1 in both seasons and sites. Application of phosphorus alone, and in combination with rhizobium inoculation increased the concentration of majority of nutrients in the rhizosphere. When the test accessions were grown at the Syferkuil and Thohoyandou study location in 2017, they showed significant differences in the concentration of N, P and K while Ca, Mg and Zn were similar in the rhizosphere. The concentrations of N, P and K were markedly higher in the rhizosphere of ACC5 compared to ACC1. In fact, the concentration of P was two-fold greater in the rhizosphere of ACC5 than ACC1. Accession 5 exhibited a markedly higher shoot dry weight, number and dry weight of pods, 100-seed weight, grain yield and harvest index compared to ACC1. P plus rhizobium inoculation, P, rhizobium inoculation affected grain yield and yield components of chickpea genotypes. This preliminary finding show that the combination of P and rhizobium inoculation affected the nutrients accumulation in the rhizosphere, shoots, grain, yield and yield components in both locations. Moreover, Thohoyandou had the highest nutrients accumulation on the rhizosphere, shoots, grain, yield and yield components compared to Syferkuil. / NRF

Thesis (Ph.D.)--University of Western Australia, 2008.

PhD (Plant Production) / Department of Plant Production / Planting chickpea genotypes at different dates within the same season may expose the crop to different environmental factors (temperature and moisture) during their vegetative and reproduction stages. Thus, knowledge of optimum planting date that minimises extreme temperature and water stress conditions during crital stages of chickpea plant development may increase biomass and grain yield. The objective of the study was to determine the effect of planting date and genotype on aboveground biomass and grain yield of chickpea under climate change scenario in North Eastern Region of South Africa. The hypothesis tested was that planting date and genotype have an effect on biomass and grain yield of chickpea under climate change scenario. Thus, a study design incorporating a combination of field and modelling experiments was set to run in 2014 and 2015 winter planting seasons at the University of Venda, South Africa. Field experiments determined the effect of planting date and genotype on chickpea flower retention and pod abortion, aboveground biomass and grain yield, water use and radiation use efficiency, whilst modelling experiments calibrated and validated the FAO AquaCrop model to simulate chickpea aboveground biomass and grain yield using climate datasets (1950 - 2100), simulated from 15 global circulation models (GCMs) under the representative carbon dioxide concentration pathways (RCP) 4.5 and 8.5. Field experiments results showed significant effect of planting date and genotype on biomass and grain yield of chickpea. Planting early, particularly under well-watered conditions appeared to be the most suitable sowing period for chickpea in this region. In contrast, late planting had lowest biomass and grain yield. The high grain yield in early planting (1.99 t ha-1) was supported by greater yield components (seed weight (13.8 gm-2) and pod weight 23 gm-2), number of pods per plant (75 pods plant-1) and harvest index (43 %)). Moreover, plant phenological factors such as plant height (46 cm) and number of branches per plant (16 branches) were also greater in early planting, with late planting recording lowest values in all the measured parameters. In addition, the greater biomass and grain yield in early planting compared with the normal and late sowings was caused by greater intercepted radiation (91%), improved flower retention (45.2%) and minimised water use (174 mm) and pod abortion (13.6%). Late maturing genotypes (Range 4 & 5) showed greater water use efficiency of grain yield (7.3 & 7.1 kg ha-1 mm-1) and had the highest radiation use efficiency of grain yield, which was on average 7.2% (0.07 g MJ-1) greater than ICCV9901, and 15.6% (0.13 g MJ-1) greater than Range 1 & 3, but this depended on soil moisture availability. vi The simulation results, indicated a significant increase in temperature (by 4.2 to 5.5 oC) over a period from 1950 to 2100. This increase lead to a concomitant increase in chickpea evapotranspiration and accumulated growing degree days. Moreover, optimal planting date for chickpea shifted from mid-month of April during 1950 to end of May in 2100 and reduced growing season length from 140 days in 1950 to 85 days in 2100. Aboveground biomass increased from 2.0 & 2.05 t ha-1 in 1950 to 4.3 & 4.57 t ha-1 in 2100, respectively in RCP 4.5 and 8.5, whilst grain yield increased from 1.07 & 1.08 t ha-1 in 1950 to 1.68 & 2.21 t ha-1 in 2100, respectively under RCP 4.5 and 8.5. Planting dates that were recommended by AquaCrop model recorded the highest increase in aboveground biomass and grain yield compared with early, normal and late planting dates. Late maturing genotypes (Range 4 & 5) showed greater grain yield and biomass, whilst early and medium maturing genotypes had low biomass and grain yield. The study recommend early planting date together with late maturing chickpea genotypes (Range 4 and 5) in the region so as to improve water use efficiency, radiation use efficiency, heat use efficiency and aboveground biomass and grain yield of the crop under the present time and under climate change scenario. The early maturing genotype (Range 1) and medium maturing genotypes (Range 3 and ICCV9901) may only be recommended under normal planting date, although there will not be any significant yield advantages compared with late maturing genotypes. The study also recommend the use of planting dates generated by AquaCrop model so as to improve biomass and grain yield when chickpea is sown under climate change scenario in Southern Africa. The yield improvement using AquaCrop recommended planting dates was partly caused by greater water use efficiency, heat use efficiency and corbon dioxide productivity. Given the potential importance of planting dates in improving current and future productivity of chickpea shown in the study, there is need to work on development of a sowing (planting date) criteria for chickpea in the / NRF

[Truncated abstract] The genetics of ascochyta blight resistance was studied in five 5 x 5 half-diallel cross sets involving seven genotypes of chickpea (ICC 3996, Almaz, Lasseter, Kaniva, 24B-Isoline, IG 9337 and Kimberley Large), three accessions of Cicer reticulatum (ILWC 118, ILWC 139 and ILWC 184) and one accession of C. echinospermum (ILWC 181) under field conditions. Both F1 and F2 generations were used in the diallel analysis. Almaz, ICC 3996 and ILWC 118 were the most resistant genotypes. Estimates of genetic parameters, following Hayman's method, showed significant additive and dominant gene actions. The analysis also revealed the involvement of both major and minor genes. Susceptibility was dominant over resistance to ascochyta blight. The recessive alleles were concentrated in the two resistant chickpea parents ICC 3996 and Almaz, and one C. reticulatum genotype ILWC 118. High narrow-sense heritability (ranging from 82 to 86% for F1 generations, and 43 to 63% for F2 generations) indicates that additive gene effects were more important than non-additive gene effects in the inheritance of the trait and greater genetic gain by breeding resistant chickpea cultivars using carefully selected parental genotypes. Current simple leaf varieties are often susceptible to ascochyta blight disease whereas varieties of other leaf types range from resistant to susceptible. The inheritance of ascochyta blight resistance and different leaf types and their correlation were investigated in intraspecific progeny derived from crosses among two resistant genotypes with normal leaf type (ICC 3996 and Almaz), one susceptible simple leaf type (Kimberley Large) and one susceptible multipinnate leaf type (24 B-Isoline). ... An interspecific F2 mapping population derived from a cross between chickpea accession ICC 3996 (resistant to ascochyta blight, early flowering, and semi-erect plant growth habit) and C. reticulatum accession ILWC 184 (susceptible to ascochyta blight, ii late flowering, and prostrate plant growth habit) was used for constructing a genetic linkage map. F2 plants were cloned through stem cuttings taken at pre-flowering stage, treated with plant growth regulator powder (0.5 mg/g indole butyric acid (IBA) and 0.5 mg/g naphthalene acetic acid (NAA)) and grown in a sand + potting mix substrate. Clones were screened for ascochyta blight resistance in controlled environment conditions using a 19 scale. Three quantitative trait loci (QTLs) were found for ascochyta blight resistance in this population. Two linked QTLs, located on linkage group (LG) 4, explained 21.1% and 4.9% of the phenotypic variation. The other QTL, located on LG3, explained 22.7% of the phenotypic variation for ascochyta blight resistance. These QTLs explained almost 49% of the variation for ascochyta blight resistance. LG3 had two major QTLs for days to flowering (explaining 90.2% of phenotypic variation) and a major single QTL for plant growth habit (explaining 95.2% of phenotypic variation). There was a negative correlation between ascochyta blight resistance and days to flowering, and a positive correlation between days to flowering and plant growth habit. The flanking markers for ascochyta blight resistance or other morphological characters can be used in marker-assisted selections to facilitate breeding programs.

Conventional production of meat products from ruminant animals in the United States requires inputs including the cultivation and nitrogen fertilization of annual grains such as corn and barley, and transportation of cattle and grain to feedlots. Consumers have concerns about the impact of feedlot conditions on animal health, and about the implications of pharmaceutical inputs such as growth hormones and antibiotics on the environment and human health. These concerns have led to a growing interest in pasturefinished meat production by consumers. Such smaller-scale livestock production systems can be healthier and lower-stress for animals, are integrated into local food systems and are more transparent to consumers, and have higher potential profitability for producers than traditional ruminant production methods. There is a strong market for pasture-finished beef products, and prices for naturally or organically raised beef have remained well above feedlot-produced product prices. There is also concern about the impact of ruminant production on the environment, including air and water pollution from feedlot production and greenhouse gasses that are emitted from ruminant animals during feed digestion. This thesis project explored the potential of a beef production system based on perennial legumes, including the non-bloating legume birdsfoot trefoil (BFT; Lotus corniculatus L.) for producing meat products from cattle while reducing concentrate feeding and methane production. The condensed tannins that are produced by BFT bind proteins in the rumen but allow them to be digested in the abomasum and intestines, which in turn leads to better utilization of forage nutrients during the finishing period and higher gains or milk production. The higher digestibility of legumes compared with grasses reduces methane emissions in cattle both through higher digestibility of the forage and through direct impacts on methanogens operating in the rumen. As reported in this thesis, steers finished on BFT gained significantly more weight per day than steers fed another perennial forage legume, cicer milkvetch, but did not gain as rapidly as feedlot-fed steers. At the end of summer grazing, the blood plasma of pasture-fed steers was lower in saturated and omega-6 fatty acids and higher in transvaccenic and omega-3 fatty acids than the blood plasma of feedlot-fed steers. When beef cows grazed grass and legume pastures, enteric methane emissions were lower on the legume pastures than the grass pasture. These results demonstrate that, compared with other feed sources, perennial legume pastures used for cattle production can improve cattle gains and reduce environmental impacts.

MSCAGR (Plant Production) / Department of Plant Production / Chickpea (Cicer arietinum L.) is an important grain legume in the world, ranking second after soybean (Glycine max L.). It accounts for a substantial proportion of human dietary nitrogen intake and plays a crucial role in food security in developing countries. Chickpea can grow in areas with low rainfall and poor soils, and thus may be an important food security crop for smallholder resource-poor farmers in the semi-arid tropics such as the dry environments of the Limpopo and Mpumalanga Provinces of South Africa. Preliminary studies showed the huge potential of chickpea production in these environments. However, no suitable genotypes have been identified and recommended for different agro-ecological zones of Limpopo and Mpumalanga Provinces. Therefore, the objective of this study was to evaluate the performance, and hence, identify the genotypes that are adapted/suitable to the contrasting agro-ecological conditions of Limpopo and Mpumalanga Provinces for production. Field experiments were conducted in the winter cropping seasons of 2016 and 2017 at Thohoyandou (University of Venda experimental station), Syferkuil (University of Limpopo experimental station) and Nelspruit (University of Mpumalanga experimental station). Ten desi chickpea genotypes were sown in a completely randomized block design replicated three times on 10 May 2016 and 10 April 2017 (Thohoyandou), 13 May 2016 and 11 April 2017 (Syferkuil) and 03 May 2016 and 24 May 2017 (Nelspruit). Plant growth characteristics were assessed by determining plant height, crop phenology, number of primary and secondary branches, and canopy cover. Yield and yield components were assessed at harvest after physiological maturity. Carbon dioxide exchange rates (CER) was determined at different growth stages using the InfraRed Gas Analyzer (IRGA). Chlorophyll content (CC) and intercepted radiation were determined weekly using the chlorophyll content meter (CCM-200 PLUS, Opti-Science, Tyngsboro, Massachusetts), and the AccuPAR, LP-80 ceptometer (Deacon Devices Ltd., Pullman, USA), respectively. Genotypes did not vary in CC at Thohoyandou in all seasons, but CC increased with stages of growth. Genotypes varied in the proportion of intercepted radiation (IR) at all measurement dates in Thohoyandou during the 2016 and 2017 growing seasons. The proportion of IR increased with growth stage, reached a peak and declined with plant age. Genotype affected photosynthesis and intercellular CO2 concentration (Ci) but did not have any significant effect on stomatal conductance (gs), transpiration (T) and Leaf Vapour Pressure Deficit (VPDL) during the 2016 season in Thohoyandou. In contrast, genotype did not affect photosynthesis, Ci, gs, T and VPDL in the 2017 season in Thohoyandou. There was no variation among genotypes on number of primary and secondary branches in Thohoyandou in both seasons. Genotypes showed no variation in plant height in the 2016 season in Thohoyandou agro-ecological condition. However, genotypes showed significant variation in plant height at 14, 70 and 84 days after emergence (DAE) in the 2017 cropping season. Moreover, genotypes showed significant variations in days to 50% flowering in Thohoyandou during the 2016 season, but showed no variations in days to 50% emergence and 75% physiological maturity. Genotypes showed no variations in days to 50% emergence, 50% flowering, 50% podding and 75% physiological maturity in the 2017 season in all locations. Genotypes showed significant variation in grain yield in Syferkuil agro-ecological condition, but showed no significant variations on all the other studied traits, while genotypes varied in 100 seed weight (SW) in Thohoyandou, but did not show any variations on the other studied traits during the 2016 season. Moreover, genotypes did not vary for all studied traits in Nelspruit during the 2016 season. The 2016 genotype and environment (G X E) interaction results showed no significant variations. However, results showed G X E interactions during the 2017 growing season suggesting that genotypes responded to environmental variation in a different way. Syferkuil had the greatest grain yield (2811 kg ha-1 and 3122 kg ha-1) in both the 2016 and 2017 growing seasons respectively, as compared to Thohoyandou and Nelspruit. These preliminary findings show that the studied genotypes responded differently in contrasting agro-ecological regions of Limpopo and Mpumalanga Provinces and that Syferkuil might be the best environment for chickpea production in this region due to its cooler temperatures. Of the genotypes evaluated the most promising genotypes are ICCV8101, ICCV3203 and ICCV4110 in these regions in terms of grain yield. / NRF

Rising fertilizer prices have led a return to the use of grass-legume mixtures to reduce N costs and improve pasture productivity. The objective of this study was to determine optimal species combinations of binary grass-legume mixtures to improve forage production and pasture nutritive value in irrigated pastures of the Intermountain West. The study was conducted at the Utah State University Intermountain Pature Research Facility near Lewiston, UT. Tall Fescue (TF), Orchardgrass (OG), and meadow brome (MB) were grown with alfalfa (ALF), birdsfoot trefoil (BFTF), and cicer milkvetch (CMV) in legume-grass mixes and monocultures at planting ratios of 25:75, 50:50, 75:25. Grass monocultures were fertilized with 0 (0 N), 67 (67 N), or 134 kg N ha-1 (134 N). Forage was harvested four times each season during 2011-2012. Forage of the mixtures and monocultures from the first and third harvests was analyzed for crude protein (CP) and neutral-detergent fiber (NDF). Average forage production of the unfertilized TF, MB, and OG monocultures was 11.03, 9.76, and 8.10 Mg ha-1, respectively. TF-ALF, OG-ALF, and MB-ALF grass-legume mixes averaged 24.0, 35.0, and 41.0% higher forage production than their respective unfertilized grass monocultures. The grass-legume mixtures with the highest CP were MB-ALF 159, TF-ALF 159, and TF-OG-159 g kg-1 and average 59, 43 and 51% higher than their respective unfertilized grass monocultures. Likewise, the mixtures with the lowest NDF were OG-ALF 453 g kg-1, OG-BFTF 469 g kg-1, and MB-ALF 480 g kg-1. These mixtures had 10, 7, and 18% lower NDF than their respective unfertilized grass monocultures. Individual harvests had similarly higher yields and CP, with lower NDF for the mixtures than the unfertilized grass monocultures. The grass-legume mixture with the 50:50 planting ratio were most productive and had high forage quality. The grass-legume mixtures had similar forage production as the grass monocultures at 134 kg N ha-1. The grass-legume mixtures also had higher CP and lower NDF than the grass monocultures. Cicer milkvetch did not perform well in irrigated pastures. Grass-legume mixtures with ALF and BFTF can be used to replace commercial N while increasing forage nutritive value.

No description available.

Chickpea (Cicer arietinum L.) production on the semi-arid Canadian Prairies is challenging due to a short growing season and low and variable moisture. The current recommended chickpea population density of 44 plants m-2 is based on preliminary studies and a narrow range of 20 to 50 plants m-2. The aims of this study were to i) determine optimum population density of varying chickpea canopy types, i.e., leaf type and growth habit, by investigating seed yield responses at 30 to 85 plants m-2 and ii) identify desirable parental traits for breeding programs by assessing growth and yield parameter responses to varying leaf types and growth habits at a range of population densities. Field experiments were conducted from 2002 to 2005. Canopy measurements and calculated variables included light interception, biomass, growth rate, seed yield, harvest index, ascochyta blight severity and radiation- and water use efficiencies. The plant density which produced the highest seed yield when averaged over years for each location for each treatment revealed that a plant density of at least 55 plants m-2 produced a 23% to 49% seed yield increase above that of the currently recommended plant density. This indicates that a higher seed yield average over the long term in spite of periodic low seed yield episodes will be more profitable to producers. Increasing plant density increased lowest pod height significantly in all except one location-year but did not explicitly increase ascochyta blight severity or decrease individual seed size. This suggests that increasing the recommended chickpea plant density on the Canadian Prairies will increase seed yield but would neither negatively impact individual seed size nor ascochyta blight severity, especially, when combined with good agronomic practices. Fern-leaved cultivars had significantly higher maximum intercepted light (62 to 91%), seed yield (136 to 369 g m-2), harvest index (0.33 to 0.53), yield-based water use efficiency (0.56 to 1.06 g m-2 mm-1) and lower ascochyta blight severity (3 to 27%) than the unifoliate cultivars in all location-years. The fern-leaved cultivars also tended to show significantly higher cumulative intercepted radiation (221 to 419 MJ m-2) and biomass (306 to 824 g m-2) but leaf type showed no consistent effect on radiation use efficiency. Cultivars with bushy growth habit generally performed better regarding maximum intercepted light (62 to 90%), cumulative intercepted radiation (233 to 421 MJ m-2), biomass (314 to 854 MJ m-2), seed yield (120 to 370 g m-2), harvest index (0.37 to 0.50), yield-based water use efficiency (0.56 to 1.06 g m-2 mm-1) and ascochyta blight severity (7 to 36%) than the erect cultivars. The overall performance of the spreading cultivar was generally intermediate between the bushy and erect cultivars except for ascochyta blight severity where the spreading cultivar exhibited significantly lower disease severity (3 to 36%). Radiation use efficiency was generally not influenced by growth habit. Increasing plant population density generally increased intercepted light, biomass and cumulative intercepted radiation on each sampling day after seeding resulting in a general increase in seed yield. Harvest index, however, remained constant and ascochyta blight severity was generally stable but radiation use efficiency decreased with increasing population density. Chickpea cultivars with fern leaves and bushy growth habit at higher than currently recommended population densities would best utilize the limited resources of the short-season Canadian prairie environment to maximize and stabilize seed yield.

Differences in pea (Pisum sativum L.) and chickpea (Cicer arietinum L.) microbial compatibility and/ or their associated farming practices may influence root fungi of the following crop and affect the yield. The main objective of this research was to explain the difference in durum wheat (Triticum turgidum L.) yield the year after pea and chickpea crops through changes in the functional diversity of wheat root fungi. The effect of fungicides used on chickpea on the root fungi of a following durum wheat crop was studied using plate culture and pyrosequencing. Pyrosequencing detected more Fusarium spp. in the roots of durum wheat after fungicide-treated chickpea than in non-fungicide treated chickpea. Plate culture revealed that the functional groups of fungi responded differently to fungicide use in the field but the effect on total community was non-significant. Highly virulent pathogens were not affected, but antagonists were suppressed. More fungal antagonists were detected after the chickpea CDC Luna than CDC Vanguard. Fungal species responded differently to the use of fungicides in vitro, but the aggregate inhibition effect on antagonists and highly virulent pathogens was similar. The effect of chickpea vs. pea previous crop and different chickpea termination times on root fungi of a following durum wheat crop was studied. The abundance of Fusarium spp. increased after cultivation of both cultivars of chickpea as compared to pea according to pyrosequencing and was negatively correlated with durum yield. Plate culture analysis revealed that fungal antagonists were more prevalent after pea than both cultivars of chickpea and chickpea CDC Vanguard increased the abundance of highly virulent pathogens. The abundance of highly virulent pathogens in durum wheat roots was negatively correlated to durum yield. Early termination of chickpea did not change the community of culturable fungi in the roots of a following durum crop. It is noteworthy that Fusarium redolens was identified for the first time in Saskatchewan and its pathogenicity was confirmed on durum wheat, pea and chickpea. The classical method of root disease diagnostics in cereals is based on the examination of the subcrown internode. I evaluated the method by comparing the fungal communities associated with different subterranean organs of durum wheat. The fungal community of the subcrown internode was different from that of roots and crown, suggesting cautious use of this method.