This dissertation is a social, political, and cultural history of the organized Chilean legal profession in the first half of the twentieth century. It explores the causes for the creation of the Chilean Bar Association and its Legal Aid Service in the mid-1920s and follows their evolution until the mid-1960s. In the early twentieth century, lawyers were dealing with growing internal and external challenges to the traditional power they had occupied in the Chilean state throughout the nineteenth century. The main internal challenge was the social and political diversification of lawyers; the external one was the social question and working- class mobilization, which represented a threat to the existing oligarchic social and political order. Both issues questioned the traditional place of lawyers in society, their formalistic understanding of the legal system, and the role of law as the main state- building tool. In response to these threats, a group of male elite Santiago lawyers founded the Bar Association in 1925, and its Legal Aid Service, in 1932. These two institutional mechanisms created and enforced a hegemonic discourse of “professional prestige” that affirmed the power of the traditional legal elite over the growing number of middle-class, leftwing, provincial, and women lawyers. These two institutions also modified the engagement of the legal profession with the state, replacing its former political engagement with a new technical, “apolitical” and “social” function of lawyers more atuned to the new welfare state. The internal power dynamics within the Chilean Bar Association and the Legal Aid Service explain the process through which the Chilean legal profession defined the “legal field,” as increasingly distinct from, but in constant tension with, the “political”and the “social” fields. Indeed, through the combined action of the Bar Association and the Legal Aid Service, lawyers were directed to deal with social inequalities, but only to the extent that this engagement did not challenge the formalistic approach to legal procedures and the liberal understanding of property rights. Furthermore, the final result of this professional project was to push lawyers to withdraw from the field of politics and from the public sphere. However, the process of imposing this notion of lawyering was constantly contested and negotiated with a diversifying constituency of rank-and-file lawyers, and subjected to increasing external pressures from the press, the state, and the lower classes. Thus, the professional model that the Bar had contributed to construct and maintain in the first half of the century would become increasingly contested in the revolutionary decades of the 1960s and 1970s. The relative success of the Bar Association in imposing its model of lawyering in the first half of the twentieth century allows us to understand why the legalistic framework that Chilean lawyers had inherited from the nineteenth century did not change over the course of the twentieth despite the momentous social and political evolution that both profession and country experienced in this period. The history of the Chilean Bar Association thus provides an institutional explanation for the continuity of ideas about the law in the face of accelerated social transformations. At the same time, by revealing the tensions and the resistance that this project faced, the history of the Bar also reveals the gears that would eventually lead to the legal profession’s historical change.

The I-BAR proteins are a family of actin regulators which include IRSp53 and Missing-In-Metastasis (MIM). These proteins possess an N-terminal I-BAR domain which associates with both the actin cytoskeleton and membrane phospholipids and is able to induce membrane curvature. Previous cell culture and in vitro studies have implicated I-BAR proteins in the regulation of actin protrusion formation, however their roles within the organism are poorly understood. We have used Drosophila melanogaster as a model system in which to study I-BAR protein function at the cellular and organismal level. Drosophila possess two I-BAR proteins, one homologous to IRSp53 and one homologous to MIM. Using full- length and truncated splice variants generated for both dMIM and dIRSp53, we have performed structure/function analysis to determine the role of specific domains in the localisation and actin modifying abilities of the proteins in both cell culture and in vivo. We found that dMIM overexpression typically promotes a lamellipodial morphology, with dMIM localising to the edges of extending lamellipodia. dIRSp53 expression induced a more filopodial phenotype in cell culture, which was not as notable in vivo, however expression of a dIRSp53 splice variant with a WH2 domain resulted instead in a predominantly lamellipodial morphology. Similar to dMIM, dIRSp53 localises to the tip of extending protrusions, albeit more transiently. We found that multiple domains contribute to the localisation and activity of dIRSp53 and dMIM. Following overexpression analysis, complementary loss-of-function analysis was performed in vivo using Drosophila mutants lacking dMIM, dIRSp53 or both genes together. Surprisingly these mutants were viable and morphologically normal. Absence of these genes individually or together did not greatly affect cell migration or actin dynamics in haemocytes or epithelial cells undergoing dorsal closure. However, a role for I- BAR proteins in axonal filopodia formation within primary neuronal cultures was apparent, as was a notable role in neuromuscular junction morphology. We have also identified potential redundancy between Drosophila MIM and the Drosophila F-BAR protein Cip4 in actin bundle regulation within embryonic haemocytes, with an additional novel role for Cip4 alone in haemocyte lamellipodial maintenance. Our results suggest that the Drosophila I-BAR proteins contribute to actin cytoskeleton regulation in vitro and in vivo, particularly within the CNS, and with novel shared functions with other BAR domain family proteins contributing to their regulation of actin cytoskeletal organisation and function.

Este trabalho tem como objetivo oferecer ao professor de matemática subsídios que permitam desenvolver atividades pedagógicas relacionadas aos conceitos básicos de divisibilidade de números inteiros. Para tanto, optou-se por desenvolvê-las de modo a possibilitar ao aluno a compreensão das ideias matemáticas envolvidas na detecção de erros de digitação em códigos numéricos utilizados nos códigos de barras e também no RG, CPF e ISBN. Vale ressaltar que tais atividades foram inspiradas nas metodologias de Resolução de Problemas e Atividades Investigativas embora, em alguns momentos, fizeram-se necessárias determinadas adaptações de acordo com o conteúdo. Assim, munidos desses elementos, os professores poderão dar aos alunos a oportunidade de desenvolver habilidades e competências da teoria elementar de números, aprimorar seus conhecimentos relacionados a situações do cotidiano e explicitar relações entre matemática e tecnologia. Este trabalho busca, também, despertar o interesse do aluno, que fica motivado ao perceber a aplicabilidade, no cotidiano, dos conceitos matemáticos aqui tratados. / The main objective of the work is to develop educational activities related to the basic concepts of divisibility of integral numbers, providing tools to math school teachers. The way those concepts were presented, creates the proper environment for the student to understand the mathematic logic behind the detection of typing errors in numerical codes, like for example: barcodes, RG, CPF and ISBN. It is noteworthy that the methodology used was inspired by problem solving and investigative activities although, adjustments were made in order to comply with the content. Thus, it is expected that teachers will be able to provide opportunities to their students to develop skills and competences on the domain of elementary number theory, improve their knowledge on daily situations and explain the relationship between mathematics and technology. This work also seeks to arouse the interest of the students, who get motivated when they realize the applicability in daily life of the mathematical concepts covered here.

The use of mobile bar code scanners is expanding to markets beyond popular manufacturing uses, such as healthcare, environmental testing and professional services. The successful interaction of users with mobile bar code scanners is of great importance from a business and technology perspective as well as from the user standpoint. Interaction problems associated with these devices may cause errors in data collection and affect job performance due to frustration, resulting in a potential impact on internal costs. The primary objective of this research was to develop design guidelines for the reengineering of keyboard designs for mobile bar code scanners. A secondary objective was to determine the effectiveness of current bar code scanner designs and how they can be related to other mobile technologies to develop a link across research areas. To accomplish these objectives, three different mobile bar code scanner keyboard designs were investigated: multiple-alphabetical, single-alphabetical and single split-Qwerty. A total of 42 subjects (18 females and 24 males) were recruited to participate in the experiment that was conducted to validate the research hypotheses. Time and accuracy data were recorded during the experiment and workload/subjective questionnaires were given to each participant following interaction with the different mobile bar code scanners. The following conclusions were reached based on the experimental results and are considered the major contributions of this research. First, an alphabetically laid out keyboard with multiple keys results in more time to find a character, a higher percentage of time spent typing incorrect lines, and a higher error rate than an alphabetically laid out keyboard with single keys. Perceived workload was also higher for a keyboard with multiple keys versus one with single keys. Second, no significant differences were found in the time to find a character, the percentage of time spent typing incorrect lines, and the error rate between keyboards with single keys, whether they are laid out alphabetically or in a split-Qwerty design. Finally, training a user on how to use a specific mobile bar code scanner keyboard layout often results in higher character rates, less time spent typing incorrect lines, and a lower error rate. / Graduation date: 2006

This dissertation consists of three parts. In the

This paper investigates the nature of the attraction of XPs to clauseinitial position in German (and other languages). It argues that there are two different types of preposing. First, an XP can move when it is attracted by an EPP-like feature of Comp. Comp can, however, also attract elements that bear the formal marker of some semantic or pragmatic (information theoretic) function. This second type of movement is driven by the attraction of a formal property of the moved element. It has often been misanalysed as “operator” movement in the past. Japanese <i>wh</i>-questions always exhibit focus intonation (FI). Furthermore, the domain of FI exhibits a correspondence to the <i>wh</i>-scope. I propose that this phonology-semantics correspondence is a result of the cyclic computation of FI, which is explained under the notion of <i>Multiple Spell-Out</i> in the recent Minimalist framework. The proposed analysis makes two predictions: (1) embedding of an FI into another is possible; (2) (overt) movement of a <i>wh</i>-phrase to a phase edge position causes a mismatch between FI and <i>wh</i>-scope. Both predictions are tested experimentally, and shown to be borne out.

The goal of galaxy classification is to understand the physical basis for the wide range in shapes and structures exhibited by galaxies in the local and high redshift universe. We present a catalog of visually classified galaxies from the Sloan Digital Sky Survey with detailed morphological classifications including bars, rings, lenses, tails, warps, dustlanes, arm flocculence and multiplicity (so called ’fine structure’). This thesis explores the importance of galaxy morphology by probing its relationship to physical properties. Our analysis includes an investigation of correlations between fine structures and AGN activity. This sample defines a comprehensive local galaxy sample which we use to study the low redshift universe both qualitatively and quantitatively. We find the stellar mass appears to be a defining characteristic of a galaxy. The break in most correlations of physical properties with morphology is due to a lack of late type, massive disk galaxies. Our analysis of the size-mass relations of galaxies as a function of morphology (T-Type) has revealed many interesting connections. We find the size-mass relation of Sa, Sab, Sb, and Sbc galaxies bifurcates into two families of objects as one moves down the sequence such that the high concentration branch exhibits a similar slope to low concentration early type (E) galaxies suggesting a closer than expected physical (possibly evolutionary) connection between the two populations. We find bar fraction is bimodal with respect to mass (at 3 x 10^10 M) and color (at g - r ∼ 0.55). The dependence is seen to intimately depend on central concentration such that objects below the transition mass with low concentrations have a higher bar fraction than objects above the transition mass which have high bar fractions for high concentration systems. In addition we find the presence of an AGN alters the behavior and abundance of barred/ringed galaxies in the high mass peak such that the bar/ring fractions increase with mass in nonactive galaxies whereas they decrease with mass in active galaxies. AGN fractions are also decreasing in the same mass range possibly implying a positive correlation between fine structure and ring formation.

FCH/CIP4 homology-Bin-Amphiphysin-Rvs (F-BAR) domain proteins are a subfamily of the Bin-Amphiphysin-Rvs (BAR) superfamily of proteins. They contain unique domains that bind and reshape the phospholipid bilayers of endosomal compartments during endocytosis. Using a functional assay for cell survival, we identified an F-BAR protein, FCH/CIP4 homology and double Src homology 3 domains 2 (FCHSD2), that confers drug resistance. Stable expression of shRNA against FCHSD2 in multiple cell types showed that loss of FCHSD2 sensitized cells to apoptosis by doxorubicin. Silencing of FCHSD2 also enhanced the ability of fibroblasts to grow colonies in culture. Mass spectrometry analysis of FCHSD2 protein complexes identified multiple interacting proteins that are involved in adhesion and endosome trafficking. We identified and confirmed a novel interaction between FCHSD2 and sorting nexin 18 (SNX18), a BAR domain protein that binds to endosomes. Our results suggest that FCHSD2 is involved in regulating cellular adhesion and cell death.

FCH/CIP4 homology-Bin-Amphiphysin-Rvs (F-BAR) domain proteins are a subfamily of the Bin-Amphiphysin-Rvs (BAR) superfamily of proteins. They contain unique domains that bind and reshape the phospholipid bilayers of endosomal compartments during endocytosis. Using a functional assay for cell survival, we identified an F-BAR protein, FCH/CIP4 homology and double Src homology 3 domains 2 (FCHSD2), that confers drug resistance. Stable expression of shRNA against FCHSD2 in multiple cell types showed that loss of FCHSD2 sensitized cells to apoptosis by doxorubicin. Silencing of FCHSD2 also enhanced the ability of fibroblasts to grow colonies in culture. Mass spectrometry analysis of FCHSD2 protein complexes identified multiple interacting proteins that are involved in adhesion and endosome trafficking. We identified and confirmed a novel interaction between FCHSD2 and sorting nexin 18 (SNX18), a BAR domain protein that binds to endosomes. Our results suggest that FCHSD2 is involved in regulating cellular adhesion and cell death.

The goal of galaxy classification is to understand the physical basis for the wide range in shapes and structures exhibited by galaxies in the local and high redshift universe. We present a catalog of visually classified galaxies from the Sloan Digital Sky Survey with detailed morphological classifications including bars, rings, lenses, tails, warps, dustlanes, arm flocculence and multiplicity (so called ’fine structure’). This thesis explores the importance of galaxy morphology by probing its relationship to physical properties. Our analysis includes an investigation of correlations between fine structures and AGN activity. This sample defines a comprehensive local galaxy sample which we use to study the low redshift universe both qualitatively and quantitatively. We find the stellar mass appears to be a defining characteristic of a galaxy. The break in most correlations of physical properties with morphology is due to a lack of late type, massive disk galaxies. Our analysis of the size-mass relations of galaxies as a function of morphology (T-Type) has revealed many interesting connections. We find the size-mass relation of Sa, Sab, Sb, and Sbc galaxies bifurcates into two families of objects as one moves down the sequence such that the high concentration branch exhibits a similar slope to low concentration early type (E) galaxies suggesting a closer than expected physical (possibly evolutionary) connection between the two populations. We find bar fraction is bimodal with respect to mass (at 3 x 10^10 M) and color (at g - r ∼ 0.55). The dependence is seen to intimately depend on central concentration such that objects below the transition mass with low concentrations have a higher bar fraction than objects above the transition mass which have high bar fractions for high concentration systems. In addition we find the presence of an AGN alters the behavior and abundance of barred/ringed galaxies in the high mass peak such that the bar/ring fractions increase with mass in nonactive galaxies whereas they decrease with mass in active galaxies. AGN fractions are also decreasing in the same mass range possibly implying a positive correlation between fine structure and ring formation.