Master of Science / Department of Biology / Brett K. Sandercock / The native grasslands of North America are highly fragmented, and remaining tracts are intensively managed for grazing. Loss of tallgrass prairie and changing land management practices has caused many grassland birds that rely on these areas for breeding to decline in population numbers. To investigate resource selection and area use requirements of the upland sandpiper, we outfitted birds with radio transmitters at the experimentally managed Konza Prairie Biological Station in northeast Kansas. Two logistical challenges for wildlife telemetry projects are: minimizing the impact of radios on survival and movement, and optimizing the duration of transmitter attachment. We compared 4 methods of radio attachment for upland sandpipers under field conditions at breeding sites in tallgrass prairie. The daily probabilities of radio retention (DRR) for our 4 attachment methods were: 0.9992 (SE = 0.0005, n = 85) for a leg loop harness, 0.9801 (SE = 0.0099, n = 11) for radios glued to clipped feathers, 0.9627 (SE = 0.0085, n = 25) for radios glued directly to feathers and 0.8538 (SE = 0.0322, n = 20) for radios glued to plucked skin. The expected duration of radio attachment ranged from 1290 days for the leg loop harness, and 6 to 50 days for the glue attachment techniques. Survival rates of males (DSR = 0.9987, SE = 0.0009, n = 53) and females (DSR = 0.9988, SE = 0.0008, n = 71) were similar, and the probability of surviving the 90-day breeding season was about 0.90 in both sexes. We recommend attaching radios with glue to clipped feathers for short-term telemetry studies and a leg loop harness if longer retention is desired. The unique landscape of Konza Prairie allowed us to investigate specific tallgrass management strategies, and their impact on the range and habitat requirements of a bird that relies on the prairie for breeding. We found that upland sandpipers have large home ranges during the breeding season (male: = 199.0 ha ± 40.5 SE, n = 21, female: = 247.7 ha ± 51.7 SE, n = 23). Male home ranges were twice as large during brood-rearing ( = 200.8 ha ± 69.1 SE, n = 9) than during the nesting period ( = 67.02 ha ± 11.84 SE, n = 14, 1 = 5.14, P = 0.023). Upland sandpipers selected home range sites that had been burned the same spring (n = 44, ²3 = 31.65, P < 0.001), but did not show preference for certain habitat types within their home range ( ²3 = 1.49, P = 0.685). During brood rearing upland sandpipers used sites with higher percentages of bare ground, herbaceous and short woody vegetation, and low amounts of vegetative litter. Management strategies for the upland sandpiper should seek to preserve large contiguous tracts of tallgrass prairie that receive a heterogeneous mix of burning and grazing.

Doctor of Philosophy / Department of Biology / Donald W. Kaufman / Glennis A. Kaufman / Little is known about nightly activity patterns of nocturnal small mammals under natural conditions, and how these activity patterns might be affected by photoperiod, season, and sex, age, and reproductive status of individuals. The main objectives of this research were: 1) to find an appropriate method for marking individual deer mice (Peromyscus maniculatus) so that their activity could be monitored remotely; 2) to design a portable activity-monitoring system to investigate temporal patterns of shelter use by deer mice under natural conditions; 3) to determine the influence of environmental conditions such as photoperiod and season on nightly activity of deer mice; and 4) to compare effects of demographic or physiological factors such as sex, age, and reproductive status on nightly activity of deer mice at artificial burrows in tallgrass prairie. In general, commencement of activity was correlated positively with timing of sunset, and time of retirement to the burrow was correlated positively with sunrise. Among adults, males first emerged from the burrow earlier and made more trips of shorter duration in a night than did females, although total duration of trips was similar. Return visits and subsequent stays typically were shorter for males than females, but total time spent in the burrow and retirement time relative to sunrise were similar for both sexes. Young deer mice emerged significantly later, made more trips of shorter duration, spent less total time outside, and retired to their burrow earlier than adults. Reproductive females emerged later, made fewer trips of generally longer duration, and spent shorter total amounts of time away from the burrow each night than non-reproductive females. Return visits of reproductive females were of longer duration than non-reproductives, but total time spent inside and time of retirement for the night did not differ relative to reproductive status. From parturition through lactation, activity of females showed a number of directional trends. Results suggest that under natural conditions, activity patterns of deer mice are highly variable but responsive to both the changing physical environment and internal conditions related to sex-specific maximization of fitness.

Doctor of Philosophy / Department of Biology / Keith B. Gido / A large scale habitat manipulation was conducted to assess the effects of establishing an emergent macrophyte, American water willow Justicia americana, on littoral reservoir communities. Coves in three large (>1,800 ha) Kansas impoundments were chosen and half planted with water willow. Sampling was conducted during the summer from 2001 to 2004. I found that water willow coves had more complex habitat as well as higher abundance and diversity of fishes, macroinvertebrates, and zooplankton than control coves. However, strong temporal variation in water levels influenced the amount of inundated water willow available in these systems. The effects of water willow on density, growth, condition, and diet of age-0 largemouth bass Micropterus salmoides were assessed. Significantly higher densities of age-0 largemouth bass were found in water willow coves, but growth, condition, and diet did not differ between water willow and control coves. Therefore, water willow was able to support higher abundances of age-0 largemouth bass than control coves without affecting growth, condition, or diet. Characteristics of age-0 largemouth bass from the water willow coves were compared to those from two small impoundments (<80 ha) with abundant macrophyte and healthy largemouth bass populations. Small impoundments had higher densities of age-0 largemouth bass than water willow coves in the three large impoundments, but individuals on average also had lower growth, condition, and fewer fish in their diet. Thus, largemouth bass populations in small impoundments may be more regulated by density dependent factors than populations in large impoundments. Overall, water willow is beneficial to littoral areas, supporting an increase in both abundance and diversity of assemblages. Finally, I used a field experiment to test the inundation and desiccation tolerance of water willow for different depths and durations. Water willow was susceptible to inundation, but resistant to desiccation. My findings provide information that can be used to select candidate reservoirs for water willow establishment based on expected water-level fluctuations.

Master of Science / Department of Biology / Brett K. Sandercock / Cattle-grazing is a dominant land use in the United States, with more than 300 million hectares of land grazed each year. The habitat changes facilitated by cattle grazing can influence resource availability and habitat selection for associated wildlife. To investigate the potential for changes in traditional livestock management to restore native grassland and riparian habitat, we evaluated biological community responses to winter-grazing and livestock exclusion at the Kansas Army Ammunition Plant in southeastern Kansas. In grassland habitats, we combined winter-grazing by domestic cattle and discontinued fertilization in an attempt to restore pastures dominated by tall fescue to native tallgrass prairie and improve habitat for grassland-breeding birds. We observed a decrease in tall fescue and an increase in native, warm-season grasses in winter-grazed pastures compared to fertilized, year-round grazed pastures. Grassland-breeding bird responses to winter-grazing were species-specific. Dickcissels preferred winter-grazed pastures, while Eastern Meadowlarks and Grasshopper Sparrows tended to prefer year-round grazed pastures. Dickcissels were negatively correlated with the presence of cattle during the breeding season and the abundance of tall fescue. Grasshopper Sparrows were negatively correlated with native, warm-season grass abundance and visual obstruction, but were positively correlated with forb abundance. Henslow’s Sparrows and Common Yellowthroats were detected breeding in low numbers on pastures that had been winter-grazed for five years. Our results suggest that winter-grazing and discontinued fertilization of agricultural grasslands can direct semi-natural plant communities toward tallgrass prairie and benefit some grassland-breeding birds. In riparian habitats, livestock were excluded from 1996 to 2005. We measured bird community responses in grazed and ungrazed sites using baseline data collected in 1996-97 and post-treatment data collected in 2004-05. Riparian bird community data were analyzed using robust design mark-recapture models that allowed us to evaluate changes in bird species richness while accounting for differences in detectability among species. We detected increases in species richness in both ungrazed and grazed treatments. We observed few differences in community vital rates between treatments; however, we did detect differences in guild responses. The changes observed within both grazed and ungrazed riparian bird communities were likely influenced by regional fluctuations in species richness and composition.

Doctor of Philosophy / Department of Biology / John M. Blair / Juniperus virginiana, an important woody plant invader in the U.S. Central Plains, has increased considerably in density and cover in large areas previously dominated by tallgrass prairie. Change in the phenology and nitrogen use efficiency of the dominant plant communities as J. virginiana replaces native prairies may lead to increased plant productivity and biomass accumulation, but may also alter the microclimate and litter quality that affect soil microbial communities responsible for key soil processes. I have focused my investigations on changes in key soil processes that could lead to differences in soil N availability, as well as changes in ecosystem C and N pools and fluxes as J. virginiana expands into native grasslands. Juniperus virginiana forest soils exhibit greater cumulative annual net N mineralization (11.52 ± 0.38 µg N g¯1 soil y¯1) compared to prairie soils (7.90 ± 0.26 µg N g¯1 soil y¯1) (F = 60.67, P = 0.016), yet slightly reduced potential soil C flux. Examination of internal soil N cycling revealed that both J. virginiana and prairie soils minimize potential soil N losses, by rapid microbial immobilization of inorganic N, and constraining nitrification via substrate limitation or environmental constraints. Leaf-level photosynthetic nitrogen use efficiency (NUE) was over a magnitude higher in the dominant grass, Andropogon gerardii, but high annual ecosystem-level NUE and greater soil N availability may contribute to the higher productivity and rapid accrual of C in newly established J. virginiana forests. Increased plant productivity and elimination of fire in J. virginiana forests have allowed at least 80,000 kg ha-1 increase in ecosystem C storage in about half a century. Soil organic C, an important long-term sink, has also increased significantly in J. virginiana forests, with approximately 34% replacement of C4 grass-derived soil C with new C from trees in the A-horizon. The observed high productivity of J. virginiana and increased N availability necessary to support continued plant biomass accumulation are possible because of substantial (~ 44%) increase in ecosystem N in measured pools, which is a likely a result of reduced volatilization of N from biomass burning, possible increased exogenous N inputs, and/or N translocation from deeper soil horizons. Reduced fire return intervals in prairie provide an opportunity for J. virginiana to establish and facilitate N accrual, which may allow this species to accelerate is own establishment through creating conditions of increased N availability and efficient utilization of N.

Doctor of Philosophy / Department of Biology / Keith B. Gido / Global climate change could lead to less frequent but more severe precipitation events in the Great Plains, altering the hydrologic regimes of streams. It is important to quantify species roles in these dynamic systems, because changes in stream communities are likely to accompany predicted changes in hydrology. The effects of species on ecosystem processes also are limited by the frequency of disturbance, because prairie streams are harsh, nonequilibrium systems characterized by a wide range of disturbances. In particular, frequent floods that reset the ecosystem to an early successional state can override the influence of consumer populations because the availability of resources is too unpredictable to maintain stable populations of those species or because species are absent following the flood. As flood frequency decreases, potential consumer effects may intensify. Using a combination of field and experimental stream mesocosm experiments, I (1) characterized the ecosystem effects of southern redbelly dace (Phoxinus erythrogaster), a grazing minnow, (2) tested the interactive effects of flood frequency and the presence of water column (red shiner; Cyprinella lutrensis) or grazing minnows (Phoxinus) on ecosystem processes, and (3) tested the effects of species loss from the grazer functional feeding group on stream ecosystem structure and function. I found that dace affected some aspects of ecosystem structure but not function, which suggested that grazer effects in prairie streams may not be consistent across taxa. In the context of flood frequency, both the water column omnivore and dace affected recovery of prairie stream primary producers following flooding disturbance by stimulating production, presumably through nutrient remineralization. However, some of these effects were transient or dependent on flood frequency, and my results indicate that consumer effects depend not only on environmental venue but also on the balance between consumptive losses and nutrient stimulation. In a comparison of the effects of removing different taxa from a grazer assemblage, the loss of crayfish, snails, or dace from a grazer assemblage did not differentially affect ecosystem processes, suggesting overlap in the ecosystem roles of these species in the context of this experiment.

Doctor of Philosophy / Department of Entomology / James F. Campbell / James R. Nechols / Theoretical and empirical ecological research has emphasized the need for understanding how animals perceive and respond to landscape structure and the importance of integrating both behavioral and landscape approaches when studying movement behavior. Knowledge of insect movement behavior is essential for understanding and modeling dispersal and population structure and developing biologically-based integrated pest management programs. My dissertation research addresses questions concerning how insects respond to landscape structure by examining movement behavior of an important stored-product pest, red flour beetle (Tribolium castaneum), in experimental landscapes. Results show that beetles modify movement behavior depending on landscape structure. Edge effects and interpatch distances may influence landscape viscosity, or the degree to which landscape structure facilitates or impedes movement, resulting in significant differences in velocity and tortuosity (amount of turning) of movement pathways, as well as retention time in landscapes with different levels of habitat abundance and aggregation. Perceptual range, or the distance from which habitat is detected, appears to be limited while beetles are moving in a landscape as they did not respond to a flour resource before physical encounter. Beetles showed differential responses to patches with various characteristics, entering covered patches more quickly than uncovered patches with more resource or the same amount of resource. Permeability of patches changed with subsequent encounters suggesting that full evaluation of patch quality may only occur after entering a patch. Beetles responded to landscape structure differently depending on the activity in which they were engaged. Distribution of movement pathways was similar to that of the habitat, but distribution of oviposition sites were significantly more aggregated than pathways and habitat. Oviposition site choice may be influenced by a complex set of factors which include previous visitation, amount of resource, travel costs, and edge effects. Insights were gained concerning how red flour beetle perceives resources, modifies search strategies, responds to boundaries, and chooses reproductive sites in patchy landscapes. This research provides new information regarding how red flour beetle interacts with landscape structure that has implications in the areas of behavioral and landscape ecology and applications in stored-product insect ecology.

Master of Arts / Department of Geography / John A. Harrington Jr / In late summer and fall, many migratory birds journey south from the North American Great Plains to spend the winter in locations with greater food availability. As spring returns, a combination of genetics and environmental cues trigger these birds to return north to their breeding grounds. Several bird migration phenology studies from Europe, Australia, and the eastern/northern United States have discovered that some bird species are returning earlier in the spring, a change that has often been correlated with a warming climate. This study aimed to be the first to analyze possible changes in both spring bird migration phenology and regional spring climate change in Kansas. First arrival dates (FADs) were collected for thirty bird species, resulting in a database with over 6,000 FAD entries. For northeast Kansas, 1997-2007 median arrival dates were calculated and compared to dates published by Dr. Richard Johnston in 1965. In southcentral Kansas, 1997-2007 and 1947-1967 median arrival dates were calculated and compared. Temperature data from spring (February through May) were also obtained from the National Climate Data Center's United States Historical Climatology Network for all stations within northeast and south central Kansas and temporal trends were analyzed with linear regression analysis. A comparison between median arrival dates in northeast Kansas found that eleven species had altered their arrival date by a potentially significant number of days. Nine of these had advanced their arrival and two showed a delay. In south central Kansas, nine species were shown to have statistically significant differences in median arrival dates between time periods. Six of these showed an advance and three showed a delay. Five species showed the same trend between both regions, with four arriving earlier by an average 9.4 days and one delaying by an average of 4.5 days. Temperature trends for both subregions in Kansas showed significant warming of 1.8-2.6°F (1.0-1.4°C) over the last century, with the largest warming observed in February and associated strongly with increasing minimum temperatures. The results from this study provide the first evidence that some bird species have recently altered the timing of their arrival in Kansas, with a greater shift toward earlier arrival dates. While the majority of these shifts were correlated with spring warming in Kansas, additional research is necessary to determine the precise cause(s) of these phenological shifts. Still, given existing literature and Intergovernmental Panel on Climate Change predictions for continued warming in the next century, continued alterations to bird migration phenology seem likely.

Master of Science / Department of Biology / Craig Paukert / I investigated the influence of anthropogenic threats and hydrologic alteration on fish assemblages within the Lower Colorado River Basin (LCRB). Life history traits of fish assemblages for individual stream segments were summarized by species presence/absence data of current (1980-2006) records. To assess anthropogenic threats, I developed a series of ecological risk indices at various scales (e.g., catchment, watershed, aquatic ecological system and upstream of aquatic ecological system) and related each index to fish life-history traits to determine the method and scale that best related to biotic metrics. Hydrologic alteration was quantified using the Indicators of Hydrologic Alteration software to calculate hydrologic alteration values using the range of variability approach. Ecological risk indices within all scales were strongly correlated (r[superscript]2>0.54, p<0.0001) to one another. Relationships between fish life history traits and ecological risk indices occurred only at the catchment and watershed scales. Strongest relationships were at the watershed scale where increased levels of anthropogenic risk were related to reduced occurrences of native, fluvial dependent species (r[superscript]2=0.12, p<0.0001) and increased occurrences of nonnative generalist species (r[superscript]2=0.22, p<0.0001). The percent agriculture was positively related to indices of alteration of low flows (r=0.401, p=0.006) while forested land cover was negatively related to alteration of low flow events (r=-0.384, p=0.008). Relationships between indices of hydrologic alteration and fish traits indicate the occurrence of piscivorous, nonnative fishes increased with alteration of low flow events whereas occurrence of fluvial dependent fishes that preferred rubble substrate decreased with alteration of low flow events (r=0.64, p=0.001). Our analysis suggests that ecological risk indices and hydrologic alteration in the LCRB are related to composition of biotic communities. Incorporating cost-effective risk indices into conservation planning will likely increase the effectiveness of conservation efforts while understanding biotic responses to modified flow regimes are a necessity in sustainable development of water resources as human populations grow and water resources decrease in the LCRB.

Master of Science / Department of Biology / Craig Paukert / I evaluated sampling strategies and the effects of dike structure modifications in the lower Missouri River to better develop sampling and mitigation strategies to protect and enhance native river fishes. Sampling occurred in the lower 1,212 km of the Missouri River during October-June (coldwater season) and June-October (warmwater season) with stationary gill nets (GN), drifted trammel nets (TN), towed otter trawls (OT), and mini fyke nets (MF) from 2003-2006. We compared probabilities of detection (p), variability (coefficient of variation; CV) in catch per unit effort, and lengths for 25 species. Over 80% of adult large-bodied fishes were collected in GN during coldwater, >90% of chub spp. (Macrhybopsis) were collected in OT, and >90% of nine small-bodied and juvenile fishes were collected in MF. Trammel nets never had the highest p during coldwater, but had the highest or equally high p for 85% of adult large-bodied fishes during warmwater. Mean CV was lowest with GN for adult large-bodied fishes; chub spp. had the lowest CV in OT. Mean lengths were typically greater in GN and TN. Large river monitoring programs might best achieve the highest p, lowest variability, and widest size range of fishes by employing GN and OT during coldwater and TN, OT, and MF during warmwater sampling periods. We also compared fish community composition and the probability an un-notched and notched dike structure and channel sand bar (referred to as channel structures) was occupied by various fish species. Few differences in species richness and diversity were evident among channel structures. Notching a dike structure had no effect on proportional abundance for any habitat guild. Catch per unit effort (CPUE) was greater at notched dikes for only three (lake sturgeon Acipenser fulvescens, paddlefish Polyodon spathula, and shovelnose sturgeon Scaphirhynchus platorynchus) of 12 great river species. Occupancy at notched dikes increased for blue catfish Ictalurus furcatus and decreased for blue sucker Cycleptus elongatus, but did not differ for 17 (81%) other species. No distinct increase in occupancy at natural channel sand bars compared to engineered dike structures was evident. Mean CPUE was higher in dike structures than channel sand bars for four great river species (goldeye Hiodon alosoides, lake sturgeon, paddlefish, and shortnose gar Lepisosteus platostomus), but did not differ for ten. Our results suggest dike structures may provide necessary habitats for many fluvial species when compared to channel sand bars, but notching did not increase abundance or occupancy of most native Missouri River fishes.