Global ETD Search

1	Characterization of Structural and Binding Properties of 4E-BP2 Lukhele, Sabelo 10 July 2013 (has links) Eukaryotic initiation factor-4E (eIF4E) controls the rate of cap-dependent translation initiation and is in turn exquisitely regulated by 4E-BPs. 4E-BP2 binds eIF4E with the highest affinity and is implicated in cancer, and metabolic and neurological disorders. Herein we use NMR, ITC and fluorescence to characterize 4E-BP2 structural and binding properties. Isolated 4E-BP2 is intrinsically disordered, but possesses some transient secondary structural propensities. eIF4E, however, is folded but has a disordered N-terminus. The eIF4E:4E-BP2 interaction is tight (Kd = 10-9 nM) and involves 4E-BP2 C-terminal and canonical binding regions, and the disordered eIF4E N-terminus. 4E-BP2 remains largely disordered upon binding to eIF4E. Noteworthy, high affinity interactions are not necessarily mediated by static structures, and 4E-BP2 binding is not the simple “disorder-to-order” transition observed in many interactions involving disordered proteins. This study offers molecular insights into 4E-BP2 functionality, and lays a foundation for development of novel therapies for cancer and neurological disorders. Translation initiation Intrinsically disordered protein Eukaryotic initiation factor 4E 4E-BP2 NMR Fluorescence 0487
2	Characterization of Structural and Binding Properties of 4E-BP2 Lukhele, Sabelo 10 July 2013 (has links) Eukaryotic initiation factor-4E (eIF4E) controls the rate of cap-dependent translation initiation and is in turn exquisitely regulated by 4E-BPs. 4E-BP2 binds eIF4E with the highest affinity and is implicated in cancer, and metabolic and neurological disorders. Herein we use NMR, ITC and fluorescence to characterize 4E-BP2 structural and binding properties. Isolated 4E-BP2 is intrinsically disordered, but possesses some transient secondary structural propensities. eIF4E, however, is folded but has a disordered N-terminus. The eIF4E:4E-BP2 interaction is tight (Kd = 10-9 nM) and involves 4E-BP2 C-terminal and canonical binding regions, and the disordered eIF4E N-terminus. 4E-BP2 remains largely disordered upon binding to eIF4E. Noteworthy, high affinity interactions are not necessarily mediated by static structures, and 4E-BP2 binding is not the simple “disorder-to-order” transition observed in many interactions involving disordered proteins. This study offers molecular insights into 4E-BP2 functionality, and lays a foundation for development of novel therapies for cancer and neurological disorders. Translation initiation Intrinsically disordered protein Eukaryotic initiation factor 4E 4E-BP2 NMR Fluorescence 0487
3	Der Einfluss endokriner Disruptoren auf das Fettgewebe der Sprague-Dawley-Ratte / The influence of endocrine disruptors on the fat tissue of the Sprague-Dawley-rat Müller, Matthias 03 July 2008 (has links) No description available. 610 Medizin, Gesundheit Medicine DPB BPA BP2 LIN PROC E2 Fettgewebe ED Insulin Glucose Leptin IGF-1 DPB BPA BP2 LIN PROC E2 fat tissue EDs insulin glucose leptin IGF-1 44.89 Endokrinologie/44.92 Gynäkologie

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