Basal rot of Narcissus caused by Fusarium oxysporum f.sp. narcissi is the major limiting factor for the increased export of ornamental bulbs from the United Kingdom. There is no known resistance to the disease amongst commercial cultivars. The purpose of this study was to develop techniques for the rapid detection and identification of the pathogen in bulbs, culture and soil, and to develop serological and biochemical tests to investigate differences amongst isolates of this forma specialis from a range of countries. Significant differences in virulence were observed between the isolates tested, two being identified as avirulent. Results suggest that there is wider morphological variation within this forma specialis than previously recorded. Isolates stored at -80°C have remained viable over a three year period. Thirty three species and varieties of Narcissus and seventeen cultivars were screened for resistance to the pathogen. Accessions were either wholly resistant, partially resistant (a variable portion of the bulbs infected) or susceptible. Accessions identified as resistant are now being used as parents in a resistance breeding programme. F. oxysporum Esp. narcissi isolates of low virulence and avirulence showed greater agglutination of microconidia with Narcissus pseudonarcissus lectin. In addition increased lectin levels were found in cultivars showing greater disease resistance; this suggests a possible host pathogen interaction. Polyclonal antisera were elicited against extracts of isolates and a specific and sensitive enzyme-linked immunosorbent assay was developed for the detection of the pathogen in bulbs. Little cross-reactivity was shown by other bulb-rotting fungi. A direct correlation was observed between positive results in the ELISA and recovery of the pathogen on selective media. The ELISA which was developed will be field evaluated with growers during the coming season. In indirect ELISA and Western blotting of fungal extracts, all fungi outside the genus Fusariwn showed consistently low binding. Isolates of F. oxysporum f.sp. narcissi could be discriminated from other Fusarium species and further split into a series of sub-groups. Avirulent isolates or isolates of low virulence showed differential binding to the PAbs. Other sub-groups were not related to virulence. Western blotting allowed greater differentiation of isolates and bands specific to F. oxysporwn f.sp. narcissi were identified. Serological detection of the pathogen in soil proved possible through the inclusion of a soil enrichment stage with a selective medium.

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Precis This graduation theses discusses about application of the concept of Basal stimulation in the work of nurses in intensive care. A theoretical part explains the concept of basal stimulation, briefly outlining the history of this concept and describes the different elements. Elements' name are include somatic, vestibular, visual, olfactory, auditory, oral and tactile - haptic stimulation. The work also highlights the importace of biographical history of the client. Research part determines the level of knowledges about basal stimulation between nurses in the ICU (intensit care units) and its use in the ICU at University Hospital in Hradec Králové. Research method is a survey. The questionnaire established author. Keywords: basal stimulation, nest location, location mummy, biographical history, communication.

Orientador: Roberto Zatz / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-07-23T06:53:14Z (GMT). No. of bitstreams: 1 Fajardo_MichelleIndiraArcos_M.pdf: 1650020 bytes, checksum: be76faba64dfbca0a9ad19977f81a84b (MD5) Previous issue date: 1997 / Resumo: A inibição crônica do óxido nítrico (NO) leva ao desenvolvimento de hipertensão arterial, lesão renal isquêmica e glomeruloesderose. A administração concomitante de Uma dieta hipersódica leva ao desenvolvimento de albuminúria maciça. Nós investigamos os mecanismos responsáveis por esse aumento de permeabilidade glomerular a macromoléculas.Ratos Munich Wistar machos e adultos receberam dietas padrão (NS) ou hipersódica (HS) e associado ou não à administração de um inibido r específico da síntese de óxido nítrico, o Nw Nitro-L-Arginina-Metil Ester (L-NAME), via oral a 70 mgjKgj dia. Após 15 e 30 dias medimos: a °albuminúria (UalbV, mgj min), a pressão arterial média (P AM, mmHg), o clearance de inulina (Ci, mIj min), o de dextran (Cd, mIj min) e o clearance fracional de dextran (8=Ci/Cd). Investigamos também a densidade de cargas aniônicas da membrana basal glomerular (MBG), perfundindo o rim com uma solução contendo ferritina catiônica e quantificando a porcentagem de depósitos de ferritina em micrografias eletrônica de glomérulos. N ossos resultados sugerem que a inibiç'ão crônica do NO quando associada a sobrecarga salina aumenta a permeabilidade glomerular através de: (1) um defeito na restrição por tamanho à filtração de macromoléculas; (2) perda da barreira eletrostática glomerular. Observamos também que 30 dias após interrupção dos tratamentos ocorre reversão dessas alterações / Abstract: We have previously described that chronic nitric oxide (NO) inhibition leads to progressive systemic hypertension and renal structural injury. Concomitant dietary salt overload and NO blockade lead to the development of massive albuminuria. Here we investigated the mechanism whereby these treatments increase the glomerular permeability to macromolecules. Adult male Munich Wistar rats were given standard-salt (55, 0.5% Na) or high salt (H5, 3.12% Na) diet and received either no treatment or the NO inhibitor Nco-L-nitroarginine metilester (NAME), 70 mg/kgl day in drinking water. At 15 and 30 days albuminuria (UalbV, mg/min), inulin clearance (Cin, mI/min), mean arterial pressure (MAP, mmHg), neutral dextran clearance (Cd, mIl min) and the fractional neutral dextran clearance (8= Cinl Cd) were measure,d. The density of fixed anionic sites at glomerular basement membrane (GBM) was also estimated by a point-counting technique, after renal perfusion with a solution containing cationic ferritin. The results suggest that chronic NO inhibition combined with H5 enhances the permeability of the glomerulus by : (1) impairing its size selectivity (by direct wall injury); (2) impairing especialIy its charge selectivity. We also observed that 30 days after the interruption of treatments all these alterations were reverted. / Mestrado / Farmacologia / Mestre em Ciências

"July 2004" / Bibliography: leaves 108-144. / v, 187 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, School of Earth and Environmental Sciences, Discipline of Environmental Biology, 2004

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 7 uppsatser.