Salinization has been identified as an increasing threat to freshwater mussel diversity in recent years. Native mussels have been observed to display reduced salinity tolerance in comparison to some invasive bivalve species, but methods by which organismal tolerance is achieved are not well understood. This study was designed to compare the behavioral and physiological responses of the native Lampsilis ovata to that of the invasive Corbicula fluminea. Lampsilis were found to exhibit strong behavioral avoidance to salinity exposure, whereas Corbicula displayed very weak avoidance to comparable salinity concentrations followed by indications of osmotic conformation through physiological mechanisms. Prolonged valve closure in Lampsilis could translate to adverse consequences related to feeding, waste removal, and energetics. Alternatively, while physiological osmotic conformation in Corbicula is associated with increased energetic costs, it allows continued respiration and feeding. These differences could convey a competitive advantage with the increasing prevalence and severity of freshwater salinization events.

<p>Plants often grow in communities closely surrounded by neighbouring plants. Plants can actively and intensely compete for resources and also anticipate competition by sensing environmental cues from the presence and identity of neighbours. Moreover, it’s been proposed that the evolution of both increased and decreased competitive ability may serve as a mechanism for invasiveness. However, still little is known about how plants integrate competitive responses when sensing multiples cues of competition and which individual competitive traits respond to the identity of competitors. In addition, whether and why the evolution of competitive traits may contribute to the ability of introduced species to become invasive is also poorly understood.</p> <p>Here I present a body of work that examined the competitive responses of a native and an invasive plant species to cues of competition and the identity of neighbours. I also examined how experimental manipulation of pot volume, to control belowground resources, affects plant growth and allocation. In one study I tested the competitive responses of the North American native, <em>Impatiens pallida</em>, to cues signalling the presence of neighbours above and belowground simultaneously in competitive environments composed of either siblings or strangers. I demonstrate that<em> I. pallida</em> can recognize siblings and shows more aggressive competitive behaviours towards strangers than kin.</p> <p>In two other studies, I compared the competitive responses of the invasive and native ecotypes of <em>Alliaria petiolata</em> to changes in density, as well as to the presence and identity of neighbours. I found that invasive ecotypes produced less competitive phenotypes especially under high density. Moreover, I found that invasive ecotypes performed better when sharing rooting space with neighbours that were siblings.</p> <p>Taken together, these results demonstrate the ability of these plant species to respond to the identity of neighbours and provide strong evidence in support of the evolution of reduced competitive ability hypothesis in invasive plant species potentially mediated by the action of kin selection in invasive ecotypes.</p> / Doctor of Philosophy (PhD)

Badges of status are supposed to have insignificant production costs, so use costs are thought to be most important in ensuring signal reliability. Use costs arise from the use of the status signal in social interactions. Social experiences that arise from the use of inappropriate signals in social interactions may drive mechanisms that result in reduced fitness for inappropriate signalers. The role of social control, probing and familiarity in producing use costs was explored. There was no evidence that social control by dominants produced a cost for cheaters and no evidence that social control by subordinates produced a cost for inappropriate signaling by Trojans. Probing produced a cost for cheating when resource value was high but not when resource value was low. Familiarity had some effect on the cost and benefit of cheating but in patterns that were not predicted. Familiarity both eliminated a benefit of cheating and reduced a cost of cheating; therefore it is uncertain how familiarity affects honest signaling. The status signal of the receiver had no effect on the cost or benefits of cheating, and there was no evidence of punishment. Social experiences have the potential to affect signal development to produce a correspondence between signal and status. The effects of social experience on signal production were examined and there was little evidence that social experience influenced bib development. Neither aggression expressed nor aggression received was not predictive of bib size. Additionally, tests on the different measures of winning experience produced conflicting conclusions regarding the relationship between winning experience and bib development.

Behavioral traits can be remarkably flexible depending on the conditions in which they are expressed, yet, in spite of this flexibility, persistent differences between individuals appear to limit the potential expression of behaviors. For example, despite evidence that parents provide variable amounts of parental care in response to changing environmental conditions, they also differ in the overall level of care they provide. I used a behavioral reaction norm approach to study individual variation in parental care behavior in free-living house sparrows (Passer domesticus). I investigated the nature of this variation by studying the relationship between different forms of parental care, the biological basis of individual variation in care, and the effect of this variation in care on offspring. First, I found a positive covariance between nestling provisioning and nest defense. Parents that provided high levels of care in one context provided high levels of care in the other context, even after accounting for measures of offspring value. Second, I sought to identify the biological sources that create and maintain consistent individual differences in the level of care a parent provides. I found that the likelihood of feeding nestlings large food items was positively associated with genetic heterozygosity, but did not find evidence that nestling provisioning was influenced by additive genetic variation in this population. Parents hatched from larger eggs provisioned offspring at a higher rate than parents hatched from smaller eggs, but there was no effect of other conditions experienced in the nest on the level of care expressed as an adult. I also tested if differences in problem-solving ability were related to differences in parental care behavior. Although I found that problem-solving parents fledged more offspring than parents that could not solve the problem, parental care was not associated with any measure of problem-solving ability. Finally, I found that individual variation in parental care reaction norms predicted the growth rate, size, and immune response of nestlings, which in turn positively affected offspring survival and recruitment. My findings reveal factors maintaining individual differences in parental care behavior and offer new insights into the causes and consequences of individual variation.

Natural enemies are members of complex ecological communities, and their ability to contribute to the biological control of pest organisms is strongly influenced by a convoluted network of ecological interactions with many other organisms within these communities. Researchers must develop an understanding of the mechanisms that shape trophic webs to predict and promote top-down effects of predators. The behavior of predators can have a strong influence on their potential as biological control agents. Web-building spiders are a useful example organism for the study of natural enemy behavior because of the experimentally tractable nature of their foraging behavior. Specifically, patterns in microhabitat utilization and web construction by spiders provide insights into foraging behavior and pest-suppression potential. In field collections, spiders were found to utilize microhabitats in a species-specific manner. Molecular gut-content analysis and a mathematical model showed that two spiders belonging to different web-building guilds differed in their dependence on microhabitat-specific prey activity-densities. In particular, the sheet-weaving guild constructed webs in microhabitats with the highest densities of springtails (Collembola). High dependence on this non-pest prey also correlated with evidence of increased intraspecific competition, and implies a potential negative effect of springtails on the consumption of pest insects, such as aphids. In laboratory two-choice assays, sheet-weaving spiders selected microhabitats and constructed webs in a flexible, stepwise manner, which allowed spiders to regulate their investment of silk resources to match the profitability of the microhabitat. Spiders also exhibited prey-specific shifts in foraging behavior, constructing webs in the presence of mobile, non-pest springtails, but utilizing active foraging tactics in the presence of sedentary, pest aphids. However, in factorial no-choice assays, pest-consumption rates were not significantly affected by the presence of non-pest springtails, indicating that prey-specific foraging-mode shifts are compatible with biological control. From these results, it is clear that the flexible foraging behavior of web-building spiders has a strong influence on their roles in ecological communities and their position within food webs. This dissertation highlights the importance of understanding the nuances of natural-enemy behavior for properly assessing and promoting biological control services.

Prior exposure to plants cues can enhance assortative mating in insects. We hypothesized that, as previously reported for females, males of Cotesia congregata would display inherent responses to plant cues that could be modified by postemergence experience and further, that males originating from two different host-plant complexes (HPCs) would display different behavioral responses to these HPCs. In no-choice contact assays with a non-host plant, searching responses of males and females increased sharply at Day 2 and remained stable through Day 4. In no-choice assays with potential host plants, males searched longer on catalpa than tobacco; responses were not modified by postemergence experience. In choice assays with both HPCs, naïve males did not display orientation preferences; however, males experienced with their natal plant preferred their natal HPC. Results indicate that postemergence experience on the natal host plant induces an orientation preference for the natal HPC and thus, can facilitate assortative mating.

The catalpa sphinx, Ceratomia catalpae, is a specialist on Catalpa trees, which produce iridoid glycosides (IGs). Whereas some trees are defoliated every year, others escape herbivory. Caterpillar populations are either heavily parasitized by the braconid wasp, Cotesia congregata, or remain unparasitized. We hypothesized that these patterns could be explained by variable IG concentrations among trees and insect responses to these chemicals. IG concentrations varied among trees. Percent defoliation was positively related to IG concentration. In comparisons of insect responses to relatively high or low IG concentrations, moths preferred to oviposit on trees with high IG concentrations. Caterpillars did not display a feeding preference nor did wasps differ in searching responses to leaf discs with high or low IG concentrations. Results indicate that observed patterns of herbivory can be explained by moth oviposition preferences for trees with high IG concentrations.

Anecdotal evidence suggested that immature lemon sharks (Negaprion brevirostris) occur at the Chandeleur Islands in southeastern Louisiana. From May 2009 to August 2011, the Nekton Research Laboratory at the University of New Orleans regularly sampled habitats at the Chandeleur Islands to confirm the presence of immature individuals of this species. During these efforts, 147 immature lemon sharks (neonates, young-of-the-year, and juveniles) were collected. Each shark was PIT tagged and a tissue sample was taken for genetic analyses. Sizes ranged from 56 – 177 cm total length. Sharks showed a preference for sandy substrate and smaller individuals showed significant preference for shallow waters. Collection data for N. brevirostris were obtained from various locations around the Gulf of Mexico for comparison. Based on a review of current literature, collection data, and microsatellite analysis it was possible to classify the Chandeleur Islands as an elasmobranch nursery ground under the definition presented by Huepel et al. (2007). Current literature and high densities of N. brevirostris suggests that the Chandeleur Islands are the only area in the northern Gulf of Mexico where neonatal and YOY lemon sharks have been recorded. Results from a combination of SPOT 5 satellite tags, PIT tags, and FLOY tags show that immature N. brevirostris at the Chandeleur Islands remain in the vicinity of the islands for several months. The abundance of juvenile sharks across years provides evidence of continuous use of these islands as a pupping area. Genetic analysis supports this information with 7 of 15 mothers returning across years. The combination of this information indicates that the Chandeleur Islands are the northern- and western-most documented nursery ground for this species. Special care should be given to this habitat as CPUE for N. brevirostris decreased significantly during this research following the 2010 Deepwater Horizon disaster and subsequent oil prevention measures. Genetic research indicates a high level of inbreeding and genetic separation between the Chandeleur Islands population and other known populations in Florida, Belize, and the Bahamas.

This study examined variation in ectoparasite density and grooming behavior of naturally occurring white-tailed deer (Odocoileus virginianus) in southwest Alabama. Stimulus-driven grooming as well as the intraspecific body size and vigilance principles of programmed grooming were tested. During the rut, males had a higher average tick (Ixodidae) density than females and exhibited complete separation of tick parasitism between non-rutting and rutting periods, supporting the vigilance principle. Stimulus-driven grooming was supported, as both fawns and yearlings had significantly higher fly (Hippoboscidae) and combined fly/tick densities than adults, and fawns oral groomed at a significantly higher rate than adults, even in the absence of allogrooming. Programmed and stimulus-driven grooming of deer examined in this study were not mutually exclusive but ectoparasite dependent.

Classical experiments on honey bee time-memory showed that foragers trained to collect food at a fixed time of day return the following day with remarkable time-accuracy. Previous field experiments revealed that not all foragers return to a food source on unrewarded test days. Rather, there exist 2 subgroups: “persistent” foragers reconnoiter the source; “reticent” foragers wait in the hive for confirmation of source availability. To examine how these foragers contribute to a colony’s ability to reallocate foragers across sources with rapidly changing availabilities, foragers were trained to collect sucrose during a restricted window for several days and observed over 3 days throughout which the feeder was empty. In 2 separate trials, activity monitoring revealed a high level of activity apparently directed at other food sources. This “extracurricular” activity showed extensive temporal overlap with visits to the feeder, indicating that honey bees can manage at least 2 different overlapping time memories.