Shallow-water hydrothermal vents occur world-wide in regions of volcanic activity. The vents located at Tutum Bay, Ambitle Island, Papua New Guinea are unique in that the vent fluids and surrounding sediments contain some of the highest concentrations of arsenic in a natural system. This study addresses the effects of the vent system on the benthic communities, focusing on the eukaryotes, macrofauna, meiofauna and bacteria. Samples were collected in November 2003 and May/June 2005. Analysis of the 2003 macrofaunal samples indicated that pH, rather than arsenic was influencing the benthic community, and that the hydrothermal influence occurred at a greater distance than expected. Results of more intensive sampling carried out in 2005 are the primary focus of this dissertation. The pore water and sediment characteristics revealed distinct physical habitats corresponding with distance from the vent. There was a trend of decreasing temperature and arsenic concentration and increasing salinity and pH with distance from the vent. The vent sediment was poorly sorted volcanic gravel, while sediments along the transect showed a gradient from fine, well sorted volcanic sands to coarser carbonate sands farther away. The macrofauna showed a trend of increasing diversity with distance from the vent and similar taxa were present in both the 2003 and 2005 samples. The vent community was dominated by the polychaete Capitella cf. capitata. The inner transect from 30 m to 140 m had low diversity. Dominant taxa included thalassinid shrimp and the amphipod Platyischnopus sp.A. The 180 m to 300 m sites had significantly higher diversity. The Danlum Bay reference site had relatively higher diversity than the nearshore transect sites and was dominated by deposit feeding polychaetes. Macrofaunal community structure was influenced by the sediment characteristics, notably by CaCO3 content, sorting and median grain size. The meiofaunal community also showed changes with distance from the vent. Chromadorid nematodes were dominant at the vent site and were a major component of the meiofauna at most sites, along with copepods. The meiofaunal community at the reference site showed greater similarity to the vent community and both sites had low abundances. Nematodes were more abundant than copepods near the vent, but copepods were more abundant farther offshore and at the reference site. Meiofaunal community structure was influenced primarily by the pore water temperature and salinity. Biological interactions with the macrofaunal community through physical disturbance and predation may also influence the meiofaunal community.  The molecular analysis of eukaryotic and bacterial diversity also revealed changes with distance from the vent. The 0 m and reference sites grouped together due to the presence of fungal sequences and the 140 m and 300 m sites grouped together due to a common molluscan sequence. Metazoans and fungi dominated the eukaryote sequences. The most abundant eukaryotic OTUs included fungi matching Paecilomyces sp. and Cladosporium cladosporioides and metazoans matching Viscosia viscosa (Nematoda) and Astarte castanea represented by 24 phyla and was dominated by Actinobacteria and γ-Proteobacteria. More bacterial phyla were present near the vent, while more overall OTUs were found at the intermediate sites along the transect. The most distant site had much lower diversity dominated by Firmicutes. The macrofaunal community had the strongest correlation with environmental variables. Comparison between the meiofauna and the metazoan sequences showed the proportion of nematodes found in both datasets were comparable, but the meiofauna analysis found a higher proportion of arthropods, while the molecular results were disproportionally high for platyhelminthes. Overall, the vents increased the complexity of the system by creating unique habitats. The extreme environment created by the hydrothermal activity maintained the surrounding habitat at an early successional stage colonized by a few opportunistic species. There was a gradation in the benthic communities away from the vent towards a more carbonate based climax community. The low pH environment had an effect on the sediment composition, which in turn influenced the benthic community. These findings can serve as a model for studying the potential effects of ocean acidification and climate change on benthic communities and marine biocomplexity.

Hypoxia is frequently detected during summer in Corpus Christi Bay, Texas, and causes significant harm to benthic organism population and diversity. Hypoxia is associated with the density stratification in the Bay but the cause of stratification is uncertain. To support the study of hypoxia and stratification, a cyberinfrastructure based on the CUAHSI (Consortium of Universities for the Advancement of Hydrologic Science, Inc) Hydrologic Information System (HIS) is implemented. HIS unites the sensor networks in the Bay by providing a standard data language and protocol for transferring data. Thus hypoxia-related data from multiple sources can be compiled into a structured database. In Corpus Christi Bay, salinity data collected from many locations and times are synthesized into a three-dimensional space-time continuum using geostatistical methods. The three dimensions are the depth, the distance along a transect line, and time. The kriged salinity concentration in space and time illuminates the pattern of movement of a saline gravity current along the bottom of the Bay. The travel time of a gravity current in the Bay is estimated to be on the order of one week and the speed is on the order of 1 km per day. Statistical study of high-resolution wind data shows that the stratification pattern in the Bay is related to the occurrence of strong, southeasterly winds in the 5 days prior to the observation. This relationship supports the hypothesis that stratification is caused by the wind initiating hypersaline gravity currents which flow from Laguna Madre into Corpus Christi Bay. An empirical physical hypoxia model is created that tracks the fate and transport of the gravity currents. The model uses wind and water quality data from real-time sensors published by HIS to predict the extent and duration of hypoxic regions in the Bay. Comparison of model results with historical data from 2005 to 2008 shows that wind-driven gravity currents can explain the spatially heterogeneous patterns of hypoxic zones in Corpus Christi Bay. / text

The tropical Indo-Pacific is the location of highest global foraminiferal biodiversity. However, the shallow-water hydrothermal system in Tutum Bay at Ambitle Island, Papua New Guinea, possesses some of the world's highest naturally-occurring [As]. Foraminifers were collected in this intriguing system in 2003 and 2005 as part of a larger project to examine the possible effects of As and other hydrothermal factors on benthic communities. Despite the high ambient [As], a diverse foraminiferal fauna was observed. Foraminferal communities were examined from surface sediment and from material adhered to rubble at locations from 1-300m from venting and from reference sites, at depths from 1-28m. From this material, 159 species were identified representing 107 genera, 55 families, 30 superfamilies, and 10 orders. Species abundances exhibit a logarithmic series distribution, with two species comprising 40%, twelve species comprising two-thirds, and 20 species comprising 80% of all identified specimens. All other species individually contributed <1% to the total community. Foraminiferal abundance and diversity were analyzed across the hydrothermal field; both increase with decreasing hydrothermal influence: decreasing sediment and pore water [As] and temperature, and increasing pH and salinity. A thorough taxonomic reference of the region was compiled, consulting appropriate original descriptions, and is herein presented, and initial steps in creating an online database of all Recent foraminifers is described. Scanning electron micrographs of the most common taxa are provided. Laboratory experiments assessed the effects of [As³?] and [As5?] on growth of Amphistegina gibbosa. Exposure to As³? and As5? at concentrations of 0- 1000µg/kg showed that As³? is approximately 2.2 times more toxic than As5?, that [As³?] of 600- 1000µg/kg is sufficient to kill or severly impair specimens on approximately two-week timescales, and that [As5?] of 1000µg/kg or [As³?] of 200µg/kg are sufficient to retard the growth of A. gibbosa on approximately four-week timescales. Over timescales of several months, cultures with extremely low [As] (2µg/kg As5? and 0µg/kg As), showed growth rates not significantly greater than high-[As] treatments, possibly due to antimicrobial/parasiticidal properties of low [As]. Foraminifers displayed an exponentially-decaying functional relationship to [As], halving their rate of growth with every 300µg/kg increase in [As³?] or 600µg/kg increase in [As5?]. Measurements of foraminiferal whole-specimen [As] via SEM-EDX, AFS, and ICP-MS revealed high [As] of ~20mg/kg for specimens near hydrothermal venting declining to background values of ~2mg/kg for distal and non-hydrothermal reference species. Laboratory-exposed specimens contain [As] of ~6mg/kg indicating an As adsorption rate of ~0.25mg/kg/wk. The major portion of foraminiferal As likely occurs in a reduced-toxicity organoarsenical form, such as arsenobetaine.

Release of methane from large marine reservoirs has been linked to climate change, as a causal mechanism and a consequence of temperature changes, during the Holocene to Late Quaternary. These inferred linkages are based primary on variation in benthic foraminifer’s singnatures. This study examines and illustrates deep sea benthic foraminifera from Holocene to Late Quaternary sample from North Pacific Ocean IODP site 890B,Cascadia Margin. Deep sea benthic foraminifera has been quantatively analyzed in samples>125 μm size fractions. Factor and Cluster analysis of the 29 highest ranked species made it possible to identify six biofacies, characterizing distinct deep sea environmental setting. The environmental interpretation of each biofacies is based on the ecology of recent deep sea benthic foraminifera. The benthic faunal record indicates fluctuating deep se condition in environmental parameter including oxygenation, surface productivity and organic food supply. The benthic assemblage show a major shift at 2 to3 kyrs BP and 6 to10.5 BP marked by major turnover in the relative abundance of species coinciding with in increasing amplitude of interstadial cycles. There are strong possibilities of methane flux in this site. Dissociation of gas hydrates and release of methane to the atmosphere could be a cause of increase in the population abundance of highly reducing environmental species, which we interpreted in our data.

To determine the relationship between the abundance and density of benthic invertebrates, and the critical shear stress of individual grain sizes, a reach along Smith Creek, was divided into ten 2m x 2m quadrants. Within each quadrant, five randomly selected clasts for each grain size ranging from 2.26 to 25.6 cm were cleaned for benthic invertebrates. Wolman pebble counts for each quadrant were also conducted and used to determine the critical Shields stress per grain size fraction from the model given by Wiberg and Smith (1987) that explicitly accounts for particle hiding/sheltering effects in mixed-bed rivers. Particle entrainment values were then compared with estimated bankfull Shields stress values to determine sediment transport potential during bankfull flow. Invertebrate abundance was strongly positively correlated with critical Shields stress up to the 18.0 cm grain size, indicating a preference for certain grain sizes; while density was positively correlated with all grain sizes present.

The objective of this thesis was to assess the physical (concentrations, durations, and sedimentation) and biological effects of total suspended solids (TSS) in Arctic lakes following in-lake construction of dikes. TSS concentration and duration estimates were applied in a Severity of Ill Effects model which predicted possible habitat degradation and a reduction of feeding of salmonids with no significant difference between stations (ANOVA, p=0.153). Benthic invertebrates collected inside suspended sediment containment curtains showed a decrease in richness and abundance (Tukey’s, p<0.05), with no effects elsewhere. Stable isotope analysis from lake trout and arctic char muscle tissue suggested no differences in isotopic signatures following TSS exposure, but stable isotope analysis of stomach contents in lake trout had a significant increase in δ15N compared to the reference basin (Tukey’s, p<0.05). These findings suggested that lake trout adapted their food sources under moderate TSS exposure and benthic invertebrates rapidly recovered to pre-disturbance values. / Agnico-Eagle Mines Limited: Meadowbank Division and the University of Guelph; Research approved by Department of Fisheries and Oceans & Nunavut Impact Review Board

No description available.

This thesis investigates how benthic fluxes of phosphorus (P), nitrogen (N), and silicon (Si) change upon oxygenation of anoxic soft bottoms in the brackish, eutrophicated Baltic Sea. Direct measurements in situ by benthic landers demonstrated that fluxes of dissolved inorganic P (DIP) from anoxic bottom sediments in the Eastern Gotland Basin are higher than previously thought (Paper I). It is argued that the benthic DIP flux has a much larger influence on the DIP inventory in the Baltic proper than the external sources. Similarly, benthic fluxes of DIP and dissolved inorganic N (DIN) from anoxic sediment in the coastal Kanholmsfjärden Basin, Stockholm archipelago, were sufficiently high to renew the pools of these nutrients below the upper mixed layer in roughly one year (Paper II). A natural inflow of oxygen rich water into the deep, and previously long-term anoxic part of Kanholmsfjärden Basin, increased the P content in the sediment by 65% and lowered DIP and dissolved silica (DSi) concentrations in the pore water. These changes, as well as the large increases in benthic effluxes of these solutes following de-oxygenation of the bottom water, suggest that they are influenced similarly by changing oxygen conditions. Experimental results in papers III and IV show that common benthic macrofauna species in the Baltic Sea can stimulate benthic release of DIN and DSi, as well as dissolved organic and particulate bound nutrients. Thus, if benthic oxygen conditions would improve in the Baltic, initial effects on benthic–pelagic nutrient coupling will change due to animal colonisation of currently azoic soft bottoms. A new box corer was designed (Paper V) which can be used to obtain highly needed virtually undisturbed samples from soft bottom sediments – if lowered slowly and straight into the bottom strata – as demonstrated by in situ videography and turbidimetry. The commonly used USNEL box corer caused severe biasing during sediment collection. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Manuscript. Paper 4: Manuscript. Paper 5: Manuscript.</p> / Baltic oxygenation project

Following the 2010 Deepwater Horizon (DWH) spill, sediment cores were analyzed from marshes at various levels of oiling to determine how deeply oil penetrated sediment in these marsh environments, and if at these sites it had quantifiably affected benthic ecosystems. Minimum mixing depths were determined from penetration of the lithogenic radionuclide 234Th, which ranged from 0.25 to 4.5 cm. Sediment accumulation rates were determined using 210Pb, with verification from 137Cs in selected cores. Lead-210 profiles revealed long-term (decadal) mixing. Bay Jimmy, Louisiana was significantly affected by the DWH oil spill, as indicated by total polycyclic aromatic hydrocarbon concentrations of up to 21,913 ppb. This is far above the level at which adverse biological effects occur (4,022 ppb). Benthic foraminifera responded to the heavy oiling by decreases to standing stock and depth of habitation relative to unoiled sites, as well as exhibiting deformities. These data clearly show that oil can be quickly mixed into salt marsh sediments, with demonstrable impacts on indigenous benthos. Further, radioisotope inventories indicated that most of the sampled sites are in a net erosional state. Should marshes containing trapped DWH oil be submerged by rising sea level, there is a great potential for the remobilization of oil.

Hemlock woolly adelgid is an invasive herbivore causing extensive mortality of eastern hemlock, an important foundation species that provides stable conditions influencing biological communities. Hemlock is often found in riparian areas and following its decline, broadleaved species, including birch, beech, and rhododendron, will replace it. These plants differ from hemlock in patterns of canopy cover and leaf properties, which influence conditions and resources within streams. My goal was to evaluate potential impacts of adelgid-induced alterations to riparian canopies and litter on benthic communities and litter breakdown in streams. I characterized benthic invertebrate communities, litter colonization and litter breakdown in streams with hemlock- or deciduous-dominated riparian canopies. Riparian canopy influenced abundance of some invertebrates, but litter species influences a range of benthic colonizers. Rhododendron and beech litter generally support more invertebrates and decomposes more slowly than birch or hemlock. When invertebrates are excluded, broadleaved litter breakdown is more hindered than hemlock breakdown. My findings suggest that invertebrates may be more affected by future increases in broadleaved litter inputs to streams than by hemlock litter loss. This is significant because benthic invertebrates are important for in-stream litter processing and are linked to aquatic and terrestrial food webs.