The following four broad aims were addressed in this study. (1) To ascertain whether the characteristics of the benthic macroinvertebrate assemblages within the different nearshore marine habitat types identified by Valesini et al. (2003) on the lower west coast of Australia differ significantly, and whether the pattern of those spatial differences matches those among the environmental characteristics that were used to distinguish those habitat types; (2) To develop a quantitative approach for classifying nearshore habitats in estuarine waters that employs readily-available data for a range of enduring environmental characteristics, and to use that approach to classify the various habitat types present in nearshore waters of the Swan-Canning Estuary on the lower west coast of Australia; (3) To test the hypothesis that the characteristics of the benthic macroinvertebrate assemblages in the in the Swan-Canning Estuary differ significantly among nearshore habitat types, and that the pattern of those differences matches that among the environmental characteristics used to distinguish those habitat types and (4) To test the hypothesis that, as a result of environmental changes in the Swan-Canning Estuary, the characteristics of the benthic macroinvertebrate assemblages at various habitats in this estuary in 1986/7 differ from those in 2003/4. To address the first aim, benthic macroinvertebrates were sampled seasonally for one year in the subtidal waters and intertidal zone (upper and lower swash zones) at the six nearshore habitat types that were identified by Valesini et al. (2003) on the lower west coast of Australia. The habitat types, which differed mainly in the extent of their exposure to wave activity and whether seagrass and/or nearshore reefs were present, had been distinguished quantitatively using values for a suite of seven statistically-selected enduring environmental characteristics. The faunal samples yielded a total of 121 species representing eight phyla, among which the Polychaeta, Malacostraca and Bivalvia were the most speciose classes and contributed ~ 38, 23 and 10%, respectively, to the total number of individuals. The total number of species and mean density of macroinvertebrates was far greater at the most protected habitat type (1), which also contained dense beds of seagrass, than at any other habitat type, i.e. 70 species and 209.2 individuals 0.1 m-2, compared to 32 species and 36.9 individuals 0.1 m-2 at the most exposed habitat type (6), which had a substrate comprised only of sand. Differences among habitat type influenced the benthic macroinvertebrate species composition to a greater extent than differences among either zones or seasons. Significantly different faunal compositions were detected among those latter two factors only at the most protected habitat type. The faunal assemblage at habitat type 1 was clearly the most distinct from those at the other five habitat types, particularly in the subtidal zone (R-statistics=0.642-0.831, p=0.1%), and was typified by five abundant polychaete species that were adapted to deposit-feeding. In contrast, the fauna at habitat type 6 was typified by four crustacean species and a species of bivalve and polychaete, whose mobility and tough external surface facilitated their survival and feeding in those turbulent waters. The extents of the differences in species composition among the six habitat types was significantly matched with that among the suite of enduring environmental characteristics that distinguished those habitat types, particularly in the case of the subtidal zone (Rho=0.676). Such results indicated that the environmental variables used to distinguish the nearshore habitat types could be used to reliably predict the types of benthic macroinvertebrate species likely to occur at any site along the lower west coast of Australia. The above biological validation of the nearshore marine habitat classification scheme developed by Valesini et al. (2003) provided the justification for the approach to the second broad aim of this study, namely to develop a quantitative scheme for classifying habitat types in the Swan-Canning Estuary. This approach was similar to that employed by Valesini et al. (2003) in that it considers that differences among habitat types are well reflected by differences in a suite of enduring environmental variables. However, it improves on that earlier method by employing a completely objective and quantitative approach. Thus, a large number of environmentally-diverse nearshore sites (102) were initially selected throughout the Swan-Canning Estuary and a suite of 13 enduring environmental variables quantified at each using remotely-sensed images of the estuary in a Geographic Information System. Such variables were chosen to reflect either (i) the type of substrate and submerged vegetation present, (ii) the extent of exposure to wave action or (iii) the location of the site within the estuary with respect to its vicinity to marine and fresh water sources. These data were then subjected to the CLUSTER routine and associated SIMPROF procedure in the PRIMER v6 multivariate statistical package to quantitatively identify those groups of sites that did not differ significantly in their environmental characteristics, and thus represented habitat types. Eighteen habitat types were identified, which were shown to well reflect spatial differences in a suite of non-enduring water quality and sediment characteristics that were measured in situ at a range of estuarine sites during both summer and winter in 2005 (Rho=0.683 and 0.740, respectively, p=0.1%). However, those latter environmental characteristics required far more time in the field and laboratory to quantify than the enduring variables used to identify the habitat types. Benthic macroinvertebrates were sampled during summer and winter in 2005 in the shallow subtidal regions (~1 m depth) at sites representing eight of the habitat types identified in the Swan-Canning Estuary. These samples contained a total of 51 and 36 species during summer and winter, respectively, and, in both seasons, represented nine phyla, namely Annelida, Crustacea, Mollusca, Sipuncula, Nematoda, Platyhelminthes, Cnidaria, Uniramia and Nemertea. The compositions of the benthic macroinvertebrate assemblages differed significantly among habitat types and, to a similar extent, between seasons (Global R-statistic=0.408 and 0.409, respectively, p=0.1%). However, the spatial differences were considerable greater in winter than in summer (Global R-statistic=0.536 vs 0.280, p=0.1%), presumably due to the greater spatial variation in particular non-enduring in situ environmental characteristics, such as redox depth and salinity. While the number of species, overall density and taxonomic distinctness of benthic macroinvertebrates also differed significantly among habitats, those variables differed to a greater extent between seasons, being greater in winter than in summer. While the measures of taxonomic distinctness tended to be greater at habitat types located in the lower to middle reaches, i.e. habitat types 6, 7, 9, 10, 13 and 18, than the upper reaches i.e. habitat types 1 and 3, the number of species and overall density reflected this trend only during winter. During summer, the mean numbers of species at habitat types 1, 3, 6 and 10 (3.4-6.0) were significantly lower than those at habitat types 7, 13, and 18 (8.8-10.9), whereas the overall density of benthic macroinvertebrates was far greater at habitat type 7 (32260 individuals 0.1 m-2)than at any other habitat type in this season (3135-18552 individuals 0.1 m-2). Overall, the greatest differences in assemblage composition occurred between those at habitat types 1 and 18 (R-statistic=0.669, p=0.1%), which were located in the uppermost region of the estuary and the lower reaches of the basin, respectively, and differed to the greatest extent in their enduring environmental characteristics. The assemblage at habitat type 1, and also that at habitat type 3, located just downstream, were relatively distinct from those at all other habitat types, particularly during winter (R-statistics=0.666-0.993, p=0.1%). The fauna at the first of these habitat types was relatively depauperate, containing low numbers of species and densities, and was characterised by the polychaetes Leitoscoloplos normalis and Ceratonereis aequisetis and the bivalve Arthritica semen. The assemblage at habitat type 3 was also characterised by those three species and the amphipod Paracorophium minor and the polychaete Boccardiella limnicola. In contrast, the assemblage at habitat type 18 was characterised by a more diverse assemblage, i.e. the polychaetes Capitella capitata, C. aequisetis, L. normalis and Pseudopolydora kempi, the amphipods, Grandidierella propodentata and Corophium minor and the bivalve Sanguinolaria biradiata. The number of species was among the highest at this habitat type during both seasons, which was also reflected in the high taxonomic diversity, and the overall density was the highest in winter and second highest in summer. Despite the above faunal differences, those between assemblages at habitat types 7 and 9, which were both located in the basin of the Swan-Canning Estuary, were similar in magnitude to those that occurred between pairs of habitat types located in two different regions of the estuary. Although both habitat types 7 and 9 were characterised by a similar suite of species, i.e. Oligochaete spp., C. aequisetis, C. capitata, C. minor, G. propodentata, L. normalis, and S. biradiata, the substantial differences in assemblage composition between these habitat types in both summer and winter (R-statistics=0.570 and 0.725, respectively) was due to marked differences in the relative contributions of each of these species. Significant and strong correlations were shown to exist in both summer and winter between the pattern of differences in the benthic macroinvertebrate assemblages among habitat types and that among the enduring environmental characteristics used to identify those habitat types (Rho=0.625 and 0.825, respectively, p=0.1%). Furthermore, these correlations were greater than those obtained between the benthic macroinvertebrate fauna and any combination of the non-enduring environmental characteristics (i.e. water quality and sediment parameters) recorded in situ at each habitat type (Rho=0.508 and 0.824, in summer and winter, respectively, p=o.1%). This demonstrates the greater capacity of surrogate enduring environmental characteristics to account for differences in the range of variables that may influence the distribution of benthic invertebrate fauna. Thus, the lists of characteristic benthic macroinvertebrate taxa produced for each of the eight habitat types studied in the Swan-Canning Estuary provide a reliable benchmark by which to gauge any future changes in those fauna. Moreover, these results indicate that the above habitat classification scheme can be used to reliably predict the types of benthic macroinvertebrate fauna that are likely to occur at any nearshore site of interest in this estuarine system. The final component of this study showed that the benthic macroinvertebrate assemblages at four sites in the middle reaches of the Swan-Canning Estuary in 2003/4 differed significantly from those recorded at the same sites in 1986/7. Such differences were reflected in (1) changes in the relative densities of a suite of ten species that were responsible for distinguishing the faunas in these two periods, (2) the absence of 22 rare species in 2003/4 (i.e. 42% of the number of species recorded in 1986/7), (3) the presence of 17 new species in 2003/4, including an abundant polychaete that is likely to have been introduced and (4) a far greater extent of seasonal variation in the number of species and densities of benthic macroinvertebrates in 2003/4. Such changes are likely to be related to lower sediment oxygen levels in certain seasons in 2003/4, as well as an altered hydrological regime due to increased temperatures and decreased rainfall in that more recent period. The fact that these changes have occurred within the Swan-Canning Estuary highlights the need for effective management tools, such as the habitat classification scheme and associated faunal survey undertaken in this study. Such data will provide a sound basis by which to examine the ways in which fauna vary spatially within the system, and allow for the establishment of comprehensive benchmarks for detecting future changes.

Nutrients, periphyton and macroinvertebrates were sampled every two months for one year to assess the effects of treated sewage effluent on the Thredbo River and to compare changes in chemical concentrations and the invertebrate community since the introduction of nutrient removal on the Thredbo sewage treatment plant. Chemically, the impact of the effluent was minor and was reduced from levels measured before the addition of nutrient removal to the Thredbo sewage treatment plant. However, the reduction may be the result of higher flows during the study period. Biologically there was no indication of nutrient enrichment during June, August and October, with differences occurring between physically dissimilar sites irrespective of position relative to the sewage effluent inflow. Large increases in periphyton and macroinvertebrates occurred in December, February and April at both open sites and those downstream of the sewage outflow. Previous studies found increases in invertebrate abundance and richness immediately downstream of the sewage inflow, but the impact was greater and extended further downstream than found in this study. Strong correlations between total phosphorus and periphyton biomass and chlorophyll-a indicate phosphorus was a limiting nutrient. Higher periphyton biomass and chlorophyll-a at all sites during the warmer months (especially at open sites with an even cobble substratum), throughout the year, indicates light, temperature and substratum type were as important as phosphorus in controlling algal growth. Low algal biomass during the high flow month of October also indicates the importance of water velocity in controlling periphyton biomass. The structure of the macroinvertebrate community was noticeably different at sites downstream of the effluent discharge, with the exception of the most downstream site, when compared to the upstream sites. The occurrence of high macroinvertebrate numbers and species richness at sites with high periphyton growth and the strong positive correlation between invertebrate grazers and periphyton measurements shows a dependent relationship between invertebrates and periphyton growth. Therefore, the physical factors of light, temperature and substratum characteristics of particular sites in the Thredbo River, may be as important in controlling the benthic community as the levels of nutrient inputs from sewage.

<p>Discoveries in recent years have disclosed the importance of marine cyano-bacteria in the context of primary production and global nitrogen cycling. It is hypothesized here that microbial mats in tropical coastal habitats harbour a rich diversity of previously uncharacterized cyanobacteria and that benthic marine nitrogen fixation in coastal zones is substantial.</p><p>A polyphasic approach was used to investigate cyanobacterial diversity in three tropical benthic marine habitats of different characters; an intertidal sand flat and a mangrove forest floor in the Indian Ocean, and a beach rock in the Pacific Ocean. In addition, nitrogenase activity was measured over diel cycles at all sites. The results revealed high cyanobacterial diversity, both morphologically and genetically. Substantial nitrogenase activity was observed, with highest rates at daytime where heterocystous species were present. However, the three habitats were dominated by non-heterocystous and unicellular genera such as <i>Microcoleus</i>, <i>Lyngbya</i>, <i>Cyanothece</i> and a large group of thin filamentous species, identified as members of the Pseudanabaenaceae family. In these consortia nocturnal nitrogenase activities were highest and <i>nifH</i> sequencing also revealed presence of non-cyanobacterial potential diazotrophs. A conclusive phylogenetic analysis of partial nifH sequences from the three sites and sequences from geographi-cally distant microbial mats revealed new clusters of benthic potentially ni-trogen-fixing cyanobacteria. Further, the non-heterocystous cyanobacterium <i>Lyngbya majuscula</i> was subjected to a physiological characterization to gain insights into regulatory aspects of its nitrogen fixation. The data demon-strated that nitrogenase activity is restricted to darkness, which called upon a re-evaluation of its diazotrophic behaviour.</p>

<p>For a complete picture of the lake ecosystem, it is necessary to understand the mechanisms regulating biomass and nutrient status (nutrient limitation, optimal supply, or surplus) of benthic algae, which are important primary producers and a food resource for grazers. This thesis gives an overview of the natural variation of benthic algae at different scales of space and time and on different substrates, and unravels some of the underlying factors. Algal nutrient status was assessed using the C:N:P (carbon:nitrogen:phosphorus) ratio of the entire natural benthic community. A review, observations, and experiments confirmed that a C:N:P ratio of about 158:18:1 (molar basis) represented an optimal nutrient supply, and that substantially higher C:N, N:P, or C:P ratios reflected algal growth limitation caused by an N or P nutrient deficiency. </p><p>Horizontal variation of benthic algal biomass and nutrient status was patchy, of similar amount for all investigated distances, substrates, and lakes, and constituted a dominant proportion of the total variation. For example, patches of nutrient limited algae were found within only 10 m distance from patches with a nutrient surplus. Thus, horizontal variation should not be neglected when sampling benthic algae in lakes. Field observations suggested an impact of wind, nutrients, and grazers on the horizontal variation. Light and nutrients might have caused the observed vertical and temporal variation. Field experiments confirmed a simultaneous control of benthic algal biomass by nutrients and grazing, mediated by light and temperature. Grazing effects were larger than nutrient effects, but the comparison of natural communities in lakes of different trophy suggested that benthic algal biomass was controlled by nutrients in the long run. </p><p>An important nutrient supply was animal excretions, causing a low C:N:P ratio of epizoon on zebra mussels, and algal communities associated with macrograzers. A field experiment revealed that 15N circulated one week longer in epizoon associated with a sessile caddisfly than in surrounding epilithon. In conclusion, the regulation of benthic algal biomass and nutrient status in lakes is complex, and benthic animals should be looked at not only as grazers, but also as a nutrient source.</p>

For a complete picture of the lake ecosystem, it is necessary to understand the mechanisms regulating biomass and nutrient status (nutrient limitation, optimal supply, or surplus) of benthic algae, which are important primary producers and a food resource for grazers. This thesis gives an overview of the natural variation of benthic algae at different scales of space and time and on different substrates, and unravels some of the underlying factors. Algal nutrient status was assessed using the C:N:P (carbon:nitrogen:phosphorus) ratio of the entire natural benthic community. A review, observations, and experiments confirmed that a C:N:P ratio of about 158:18:1 (molar basis) represented an optimal nutrient supply, and that substantially higher C:N, N:P, or C:P ratios reflected algal growth limitation caused by an N or P nutrient deficiency. Horizontal variation of benthic algal biomass and nutrient status was patchy, of similar amount for all investigated distances, substrates, and lakes, and constituted a dominant proportion of the total variation. For example, patches of nutrient limited algae were found within only 10 m distance from patches with a nutrient surplus. Thus, horizontal variation should not be neglected when sampling benthic algae in lakes. Field observations suggested an impact of wind, nutrients, and grazers on the horizontal variation. Light and nutrients might have caused the observed vertical and temporal variation. Field experiments confirmed a simultaneous control of benthic algal biomass by nutrients and grazing, mediated by light and temperature. Grazing effects were larger than nutrient effects, but the comparison of natural communities in lakes of different trophy suggested that benthic algal biomass was controlled by nutrients in the long run. An important nutrient supply was animal excretions, causing a low C:N:P ratio of epizoon on zebra mussels, and algal communities associated with macrograzers. A field experiment revealed that 15N circulated one week longer in epizoon associated with a sessile caddisfly than in surrounding epilithon. In conclusion, the regulation of benthic algal biomass and nutrient status in lakes is complex, and benthic animals should be looked at not only as grazers, but also as a nutrient source.

Discoveries in recent years have disclosed the importance of marine cyano-bacteria in the context of primary production and global nitrogen cycling. It is hypothesized here that microbial mats in tropical coastal habitats harbour a rich diversity of previously uncharacterized cyanobacteria and that benthic marine nitrogen fixation in coastal zones is substantial. A polyphasic approach was used to investigate cyanobacterial diversity in three tropical benthic marine habitats of different characters; an intertidal sand flat and a mangrove forest floor in the Indian Ocean, and a beach rock in the Pacific Ocean. In addition, nitrogenase activity was measured over diel cycles at all sites. The results revealed high cyanobacterial diversity, both morphologically and genetically. Substantial nitrogenase activity was observed, with highest rates at daytime where heterocystous species were present. However, the three habitats were dominated by non-heterocystous and unicellular genera such as Microcoleus, Lyngbya, Cyanothece and a large group of thin filamentous species, identified as members of the Pseudanabaenaceae family. In these consortia nocturnal nitrogenase activities were highest and nifH sequencing also revealed presence of non-cyanobacterial potential diazotrophs. A conclusive phylogenetic analysis of partial nifH sequences from the three sites and sequences from geographi-cally distant microbial mats revealed new clusters of benthic potentially ni-trogen-fixing cyanobacteria. Further, the non-heterocystous cyanobacterium Lyngbya majuscula was subjected to a physiological characterization to gain insights into regulatory aspects of its nitrogen fixation. The data demon-strated that nitrogenase activity is restricted to darkness, which called upon a re-evaluation of its diazotrophic behaviour.

Benthic invertebrates play important roles as feeding resources for many organisms in different food webs. Shifts in predation of these organisms can generate cascading effects and potentially lead to the disappearance of one or more species from a site. Cascading effects can bring impacts to organisms who aren’t even directly involved, why studies in this field are important for understanding sudden changes in ecosystems. I examined the predation from fish and waterfowl on benthic invertebrates in the shallow and eutrophic Lake Tåkern in the plains of Östergötland County, southern Sweden. The study was experimental and used exclosures (three types, eight replicates) in the shape of 130-liter cages to examine the composition of invertebrates. It was conducted during early spring, a relatively unexamined period for this kind of study. I found no significant differences in the control treatment compared to any of the other treatments regarding biodiversity. The invertebrate fauna was dominated by a small number of species, with a relatively patchy spread throughout the sediment area. Furthermore, the results indicate that the fish hadn’t had the time to properly activate their predatory habits due to prolonged winter temperatures. The waterfowl were considered too absent during the test period to affect the invertebrate community. Future studies in this area are recommended to work with a greater sampling area, to reduce the influence of extreme values.

In der vorliegenden Arbeit werden für ein stark anthropogen beeinflusstes Einzugsgebiet die komplexen Zusammenhänge zwischen Witterung, Landnutzung, Erosion, Gerinneabfluss, Stofffrachten und Makrozoobenthos analysiert. Dafür wurde eine Messstation eingerichtet, an der Fließgeschwindigkeit, Trübung, pH Wert und Leitfähigkeit kontinuierlich gemessen wurden. Mit Hilfe eines durchflussgesteuerten Probennehmers wurden Wasserproben entnommen und im Labor auf verschiedene Inhaltsstoffe (Nährstoffe, Schwermetalle) analysiert sowie die Konzentration von Feinsediment bestimmt. Es konnte eine Beziehung zwischen Trübung und Sedimentkonzentration abgeleitet werden. Die chemischen Analysen zeigten einen komplexen Zusammenhang zwischen der Erosion von landwirtschaftlichen Flächen, dem Stoffeintrag von versiegelten Arealen (Verkehrsflächen, Dächer) aber auch witterungs- und vegetationsbedingte saisonale Effekte. Ein Vergleich der Eigenschaften der Schwebstoffe/Feinsediment im Landwasser mit denen von Ackerboden (Maisfelder) zeigte, dass diese die „chemische Handschrift“ der Ackerböden trugen. Mit Hilfe der Modelle EROSION 3D und ICECREAM wurden die abgetragenen Bodenmengen für das Einzugsgebiet bzw. für ausgewählte Hänge quantifiziert. EROSION 3D überschätzt den Bodenaustrag aus dem Untersuchungsgebiet deutlich. Das Modell ist ungeeignet, um für das Einzugsgebiet reale Abtragswerte mit den zur Verfügung stehenden flächenhaften Eingangsdaten zu berechnen. Bereits für Einzelhänge werden zu große Bodenabträge berechnet. Verstärkend kommt hinzu, dass auf Grund der Modellannahme jeder Partikel, der das Gewässer erreicht auch aus dem Einzugsgebiet heraustransportiert wird. Das Modell kann empfohlen werden, um erosionsgefährdete Flächen auszuweisen. In der Untersuchungsperiode von 2009 bis Juli 2011 wurde an Hand von 57 Driftfängen mit unterschiedlichen hydrologischen Randbedingungen die Wirkung der Fließgeschwindigkeit auf den Übergang benthischer Invertebraten in die freie Welle identifiziert. Aus Uferbereichen gelangen zufällig terrestrische Invertebraten in das Gewässer, deren Anteil sich durch gewässernahen Oberflächenabfluss in Folge von Niederschlagsereignissen erhöht. Die Driftdichte nimmt ab Fließgeschwindigkeiten von 0,3 m/s deutlich zu. Oligochaeta, Cyclopoida und Dipolstraca driften verstärkt bei Fließgeschwindigkeiten >0,6 m/s.

The subject of this study was to investigate the species composition and spatial distribution pastern of iving (Rose Bengal stained) benthic foraminifera in sediment surface samples (0-1 cm) off the Southwestern Taiwan and Dongsha Atoll. The main objectives of this study was to document the living benthic foraminiferal assemblages off the Southwestern Taiwan and Dongsha Atoll and to examine their distribution in relation to environmental parameters. Four benthic foraminiferal biofacies were recognized within the substrate type in Dongsha Atoll: A1 (Fine-grained) is represented by Pseudomassilina spp. - Quinqueloculina parkeri, A2 (Fine-grained) is represented by Hauerina spp. - Pseudomassilina spp., B1 (Coarse-grained) is characterised by Calcarina spp. - Quinqueloculina spp., B2 (Coarse-grained) is characterised by Amphistegina spp. - Quinqueloculina spp. Sediment surface samples off the Southwestern Taiwan during May 2009 led to the identification of four assemblages. Assemblage A is characterised by Ammonia spp. - Bolivina spp., Assemblage B between is characterized by Asterorotalia spp. - Globobulimina spp., Assemblage C is represented by Cibicides spp. - Nummulites spp., Assemblage D is represented by Ammonia spp. - Cibicides spp. Sediment surface samples off the Southwestern Taiwan during Nov. 2009 led to the identification of three assemblages. Assemblage A is dominated by Ammonia spp. - Cibicides spp., Assemblage B is characterised by Ammonia spp. - Bolivina spp., Assemblage C is characterised by Asterorotalia spp. - Bolivina spp. Stained benthic foraminifera form Dongsha Atoll correlated with current wave, collected site, coarse fraction and TOC contents. Living benthic foraminifera off the Southwestern Taiwan seems not to be related with TOC contents and coarse fraction, the assemblages were found to be more related to tidal energy and lateral transport.

The objectives of this study are to investigate the biodiversity in different unit of treatment systems, and to detect the function in a salt water type of constructed wetland. We investigated the benthic invertebrate community in different stage from 2010 July to 2011 May, while the parameters of TKN, NH3-N, organic nitrogen, TP, TOC and particle size were measured in the sediments of each sampling site at the same time in the wetland system. The results show that concentrations of organic matter and nutrients in the sediments were increased with time monthly. In May of 2010, the concentration of NH3-N were found the highest one(ANOVA, p<0.05). The particle size in sampling site 1 were the highest (ANOVA, p<0.05), while in November of 2010 all sampling sites were found exhibiting significantly different with other months (ANOVA, p<0.05). Further more, for the diversity of benthic invertebrate, we found that the parameter of the temperature was strongly negatively related to the species diversity, species abundance and species evenness, respectively (r=-387[H¡¦]¡F-533[d]¡F-438[J¡¦] ). The species diversity was increased with organic nitrogen concentrations in the sediments (r=0.492[TKN]¡F0.408[NH3-N]¡F0.493[org-N]), and were negatively related to the parameters of DO and particle size(r=-0.402[Particle size]¡F-0.287[DO] ). In addition, PCA shows that the parameters of particle size¡Borg-N¡BNH3-N¡BTKN and TOC were all important factors. Generally, it was concluded that the constructed systems, which is functioned of wetland was wastewater treatment mainly, exhibit no significant function in biodiversity.