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Les mitochondries sont des organites essentiels pour la production d'énergie cellulaire grâce à la synthèse d'ATP au cours des étapes de phosphorylations oxydatives (OXPHOS). Les complexes de la chaine respiratoire sont en partie codés par le génome mitochondrial (ADNmt), dont la structure est très sensible aux facteurs exogènes ou endogènes. De nombreuses mutations de l'ADNmt sont associées à des dysfonctionnements de la chaine respiratoire conduisant à des pathologies. La production d’Espèces Oxygénées Réactives (EOR) mitochondriale est la principale source de dommages à l’ADNmt. Une voie de réparation particulière, le système de réparation par excision de bases (BER) est mis en oeuvre dans ce cas. Nous avons, au cours de notre étude, analysé le système BER mitochondrial chez la drosophile. Dans une première approche, nous avons caractérisé de manière globale par une technologie de puces à ADN un ensemble de glycosylases et endonucléases impliquées dans la voie BER mitochondriale et comparé leur variation au cours du vieillissement. Cette étude a été complétée par une analyse transcriptionnelle sur des modèles de drosophiles mutantes pour des enzymes spécifiques de la voie BER, ceci afin de déterminer les éventuelles interactions transcriptionnelles entre les acteurs de cette voie. L’ARNm de Parp présentait de fortes variations dans les différents contextes mutants testés. C’est une molécule essentielle de la réparation BER. Elle a fait l’objet dans un deuxième temps, d’une étude plus approfondie. Dans le modèle des cellules S2, PARP bien que majoritairement nucléaire est également présent dans la mitochondrie. Le comportement différentiel des deux variants ARNm de Parp a pu être mis en évidence lors de stress cellulaires. Les isoformes protéiques de PARP observées dans nos études apparaissent différentes de celles habituellement décrites dans la littérature. Cet aspect a été discuté. / Mitochondria are key organelles mainly devoted to energy production through ATP synthesis. Such a function is permitted by oxidative phosphorylation (OXPHOS) within mitochondria inner membrane. Key components of the OXPHOS processes are encoded by mitochondrial DNA (mtDNA) that is particularly sensitive to exogenous or endogenous insults. As a result, mtDNA mutations are often correlated with OXPHOS dysfunction leading to diseases. ROS production in mitochondria is the main source of mtDNA damage. Such DNA damages are mainly taken over by BER systems within mitochondria. In this study, we focused on this peculiar mitochondrial DNA repair system in Drosophila. In a first step, we analysed in a comprehensive manner through microarray, most glycosylases and endonucleases involved in mitochondrial BER and compared their evolution during aging. Using mutant flies for specific BER enzymes, we started to decipher some of the transcriptional interactions between key BER actors. In a second step, Parp molecule was further studied due its changes in all mutant contexts and for its importance in several cellular processes. We described its nuclear but also its mitochondrial location in S2 cells. Interestingly, two Parp mRNA variants were observed showing distinct regulations following stress induction. However, PARP protein isoforms observed in this study were different compared to what was described in literature. This discrepancy is discussed.

Submitted by Carolina Neves (carolinapon@uefs.br) on 2016-08-09T01:01:22Z No. of bitstreams: 1 Karine_Souza_de_Almeida_Dissertacao_Final.pdf: 13569698 bytes, checksum: 81752dfa3be4ee9493654f5c61524c5a (MD5) / Made available in DSpace on 2016-08-09T01:01:22Z (GMT). No. of bitstreams: 1 Karine_Souza_de_Almeida_Dissertacao_Final.pdf: 13569698 bytes, checksum: 81752dfa3be4ee9493654f5c61524c5a (MD5) Previous issue date: 2016-05-24 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / By using the Orthogonal Frequency Division Multiplexing (OFDM), it is possible to transmit by a channel a more robust signal and less susceptible to interferences. When such technique, based on orthogonal subcarriers, is combined with the use of propagation models, which are relevant tools for simulating the signal behavior in respect to environmental parameters and antenna configurations, it is feasible to have an evaluation about the performance of these systems, improving their project phase. Therefore, this work presents the use of OFDM technique applied to two propagation models: a two-ray geometric model and a microcellular model adapted to the Paulista Avenue, S?o Paulo, SP, having as objective the analysis of a mobile communication system through Bit Error Rate (BER). In the developed application using the MAtrix LABoratory (MATLAB) software, the mobile communication system based on OFDM admits several scenarios during the simulation, so that scenarios are related to positioning of the antennas, heights, type of modulation, size of the bit sequence to be sent, and others. The adopted OFDM architecture has been the IFFT/FFT and based on it, the system performs the transmission and reception of a bit set through a channel with interference by using different digital modulations, making appropriate comparisons. / Atrav?s da utiliza??o da t?cnica de Multiplexa??o por Divis?o de Frequ?ncias Ortogonais (OFDM, do ingl?s, Orthogonal Frequency Division Multiplexing), ? poss?vel transmitir por um canal um sinal mais robusto e menos propenso a interfer?ncias. Quando tal t?cnica, baseada na utiliza??o de subportadoras ortogonais, ? aliada ao uso de modelos de propaga??o, que s?o ferramentas relevantes para a simula??o do comportamento do sinal em rela??o aos par?metros do ambiente e as configura??es das antenas, ? poss?vel ter uma avalia??o a respeito do desempenho desses sistemas, melhorando a fase de projeto dos mesmos. Sendo assim, este trabalho apresenta o uso da t?cnica OFDM aplicada a dois modelos de propaga??o: um modelo geom?trico de dois raios e um modelo microcelular adaptado para a Avenida Paulista, S?o Paulo, SP, possuindo como objetivo a an?lise de um sistema de comunica??o m?vel atrav?s do c?lculo da Taxa de Erro de Bit (BER, do ingl?s, Bit Error Rate). Na aplica??o desenvolvida utilizando o software MATLAB (MATrix LABoratory), o sistema de comunica??o m?vel baseado em OFDM assume diversos cen?rios durante as simula??es, de forma que esses cen?rios s?o relacionados ao posicionamento das antenas, alturas, tipo de modula??o, tamanho da sequ?ncia de bits a ser enviada, entre outras. A arquitetura OFDM adotada foi a IFFT/FFT e baseado nela, o sistema realiza a transmiss?o e a recep??o de um conjunto de bits por um canal com interfer?ncias utilizando diferentes modula??es digitais, fazendo as devidas compara??es.

This thesis deals with Matlab application developed for simulation of the DVB-T channel coder and decoder. The first part of this thesis includes description of terrestrial digital video broadcasting system and comparison with analogue television. Channel coding and OFDM modulation, used in the DVB-T standard, is described in detail. Application developed in the Matlab environment is described in the second part. The application simulates data transfer of the DVB-T system. Results of the simulated transmission, using developed application are presented in the last part. Namely dependence of the BER on the S/N ratio, using various coder settings, was examined. Maximal possible data rate was determined for these various setting. All obtained values are graphically represented.

It is known that a matched filter is optimal for a signal corrupted by Gaussian noise. In a wireless environment, the received signal may be corrupted by Gaussian noise and a variety of other channel disturbances: cochannel interference, multiple access interference, large and small-scale fading, etc. Adaptive filtering is the usual approach to mitigating this channel distortion. Existing adaptive filtering techniques usually attempt to minimize the mean square error (MSE) of some aspect of the received signal, with respect to the desired aspect of that signal. Adaptive minimization of MSE does not always guarantee minimization of bit error rate (BER). The main focus of this research involves estimation of the probability density function (PDF) of the received signal; this PDF estimate is used to adaptively determine a solution that minimizes BER. To this end, a new adaptive procedure called the Minimum BER Estimation (MBE) algorithm has been developed. MBE shows improvement over the Least Mean Squares (LMS) algorithm for most simulations involving interference and in some multipath situations. Furthermore, the new algorithm is more robust than LMS to changes in algorithm parameters such as stepsize and window width. / Master of Science

International Telemetering Conference Proceedings / October 18-21, 2004 / Town & Country Resort, San Diego, California / The impact of frequency selective multipath fading on the bit error rate performance of ARTM Tier-1 waveforms (FQPSK and SOQPSK) is derived and analyzed. In the presence of a strong specular reflection with relative magnitude |Γ1|, the ARTM Tier-1 waveforms suffer a loss in performance of (1 - |Γ1)^(-4√(|Γ1|)) for |Γ1| < 0:5 and a relatively high error floor at approximately 10^(-2) for |Γ1| ≥ 0.5. The ARTM Tier-1 waveforms possess twice the spectral efficiency of PCM/FM, but exhibit a greater loss and higher error floors than PCM/FM for the same multipath conditions and signal-to-noise ratio.

International Telemetering Conference Proceedings / October 21, 2002 / Town & Country Hotel and Conference Center, San Diego, California / Feher Keying (FK) signals are clock shaped baseband waveforms with the potential to attain very high spectral efficiencies. Two FK signals which have different level rectangular waveforms (named as FK-1) or sinusoidal waveforms (named as FK-2) for two binary symbols are considered in this paper. These signals have periodic components in the time domain. Therefore they have cyclostationary properties. This means that spectral correlation exists in the frequency domain. For each type of waveforms, spectral correlation has been investigated. FK signals can be expressed mathematically into two parts in the frequency domain – discrete part and continuous part. The discrete part has one or more discrete impulse(s) in their spectra and the continuous part has periodically the same shape of harmonics in their spectra. The correlations of their spectra have been obtained mathematically and by simulation. It is shown that FK signals have high correlation related to the symbol rate. Finally, some suggestions how these properties can be used to improve their performance by devising better demodulators are discussed. These properties can be used for interference rejection at the receiver, which results in low bit error rate performance.

ITC/USA 2008 Conference Proceedings / The Forty-Fourth Annual International Telemetering Conference and Technical Exhibition / October 27-30, 2008 / Town and Country Resort & Convention Center, San Diego, California / This paper describes the use of orthogonal space-time block codes to overcome the performance and complexity difficulties associated with the use of Shaped Offset QPSK (SOQPSK) modulation, a ternary continuous phase modulation (CPM), in multiple-input multiple-output telemetry systems. The orthogonal space-time block code is applied to SOQPSK waveforms in the same way it would be applied to symbols. The procedure allows the receiver to orthogonalize the link. The main benefits of this orthogonalization are the easy realization of the transmit diversity for the offset-featured SQOSPK, and the removal of the noise correlation at the input to the space-time decoder and the elimination of I/Q interference when space time orthogonalization is applied to the symbol level.

ITC/USA 2007 Conference Proceedings / The Forty-Third Annual International Telemetering Conference and Technical Exhibition / October 22-25, 2007 / Riviera Hotel & Convention Center, Las Vegas, Nevada / The accuracy of channel estimation plays a crucial role in the demodulation of data symbols sent across an unknown wireless medium. In this work a new analytical expression for the channel estimation error of a multiple input multiple output (MIMO) system is obtained when the wireless medium is continuously changing in the temporal domain. Numerical examples are provided to illustrate our findings. Space-time (ST) coding using Continuous Phase Modulation (CPM) has spectral advantages relative to linear modulations. In spite of the spectral benefits, Space-Time Trellis Codes (STTC) using the CPM implementation of Minimum Shift Keying (MSK) scheme has inherent inphase and quadrature interference, when the received complex baseband signal is the input into the matchfilter to remove the shaped sinusoid pulses. In this paper a novel optimum transmitting and detecting structure for STTC-MSK is proposed. Treating the Alamouti scheme as an outer code, each STTC MSK waveform frame is immediately followed by the orthogonal conjugate waveform frame at the transmit side. At the receiver first orthogonal wave forming is applied, then a new time-variant yet simple trellis structure of the STTC-MSK signals is developed. This STTC-MSK detector is absolutely guaranteed to be I/Q interference-free and still keeps a smaller computation load compared with STTC-QPSK. Simulations are made over quasi-static AWGN fading channel. It is shown that our detector for ST-MSK has solved the I/Q interference problem and has around 2.8 dB gain compared with the Alamouti Scheme and 3.8 dB gain for bit error rate at 5 X 10^(-3) in a 2 by 1 Multiple Input Single Output system.

International Telemetering Conference Proceedings / October 23-26, 2000 / Town & Country Hotel and Conference Center, San Diego, California / Shock and vibration testing was performed on the Metrum-Datatape Inc. 32HE recorder to determine its viability as an airborne instrumentation recorder. A secondary goal of the testing was to characterize the recorder operational shock and vibration envelope. Both flight testing and laboratory environmental testing of the recorder was performed to make these determinations. This paper addresses the laboratory portion of the shock and vibration testing and addresses the test methodology and rationale, test set-up, results, challenges, and lessons learned.