Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting women of reproductive age. The major features of PCOS include polycystic cysts, excess androgens, and polycystic ovaries. Dihydrotestosterone (DHT) is an androgen, and when in excess causes associated with PCOS used to induce PCOS in animal models. In excess, DHT is associated with PCOS features. This study aimed to induce PCOS-like symptoms in Drosophila Melanogaster by supplementing DHT in the diet. The reproductive and metabolic features of PCOS were investigated. Fecundity rates, triglyceride levels, and gene expression levels of Toll and Med genes were assessed. The mean levels of triglycerides (P=0.023) had significantly increased in the high-dose DHT group (F2 generation). The mean expression levels (P=0.02) of the Toll gene were significantly increased in the low-dose DHT group. The treatment of DHT, however, did not affect the fecundity rates of the Drosophila. These findings suggest that DHT induces PCOS-associated metabolic features of lipid alterations and chronic low-grade inflammation. Assessment of the impact on fertility did not give conclusive results thus additional methodologies could be considered in studying the reproductive function. In conclusion, the results obtained in this study highlight the potential of using Drosophila as a model in PCOS research. / <p>Det finns övrigt digitalt material (t.ex. film-, bild- eller ljudfiler) eller modeller/artefakter tillhörande examensarbetet som ska skickas till arkivet.</p><p>There are other digital material (eg film, image or audio files) or models/artifacts that belongs to the thesis and need to be archived.</p>

Polycystic ovary syndrome is a common endocrine disorder that hinders women’s metabolism and reproductive health characterized by elevated androgen levels, polycystic ovaries, infertility, hirsutism, inflammation, obesity and insulin resistance. Research suggests mitochondrial dysfunction as an underlying cause of PCOS. This study aimed to develop a PCOS model of Drosophila melanogaster by induction of androgens (testosterone). The eggs of the wild-type stock flies were exposed to ethanol (control) and varying doses of testosterone (1-10mg/kg) and examined throughout development. Androgen and ethanol exposure caused alterations in the external morphology and ovarian appearance suggesting a link to PCOS-related symptoms. Fertility measured by fecundity rates decreased by 32.6% in androgen treated flies, though statistical tests revealed no significant differences between groups. Androgen exposure altered the mitochondrial gene expression but with no statistical difference between conditions in all genes (p values: MTCO1 = 0.368; SOD1 = 0.276; ATPsynthase = 0.102). MTCO1 showed approximately a 99% difference in low and high testosterone groups from control. SOD1 increased by 7645.72% and 98.8%, and ATPsynthase by 319.9% and 703.4%in low and high testosterone conditions, respectively. Triglyceride levels were elevated in androgen groups across F1 and F2 generations, indicating potential metabolic disruptions. Significant differences revealed between control and high testosterone (F1, p = 0.04; F2, p = 0.006) but not in low testosterone (F1, p = 0.402; F2, p = 0.328). Lack of significant findings renders the results unreliable. Future research with broader gene panels and improved methologies are required to validate the model's applicability for PCOS research. / <p>Det finns övrigt digitalt material (t.ex. film-, bild- eller ljudfiler) eller modeller/artefakter tillhörande examensarbetet som ska skickas till arkivet.</p><p>There are other digital material (eg film, image or audio files) or models/artifacts that belongs to the thesis and need to be archived.</p>

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Knäets artär, arteria (a.) poplitea, kan drabbas av aneurysm, vanligen om personen redan har ett aortaaneurysm. Kärlväggen påverkas av träning på olika sätt. Enligt olika studier ökar eller minskar a. popliteas lumendiameter av aerob träning. Syftet med denna studie är att se om regelbunden aerob träning påverkar lumendiametern i a. poplitea. Med hjälp av ultraljud hararteria popliteas lumendiameter mätts hos totalt 31 frivilliga personer, där hälften var tränade och resten var otränade, både kvinnor och män. Insamlad data har analyserats med IBM SPSS version 27. Denna studie bekräftar det tidigare studier sagt, att män har större lumendiameter i a. poplitea än kvinnor. Lumendiametern i a. poplitea är större i dess proximala och mediala delar, än i dess distala del. Studien visar inga signifikanta skillnader i a. popliteas lumendiameter mellan personer som tränar regelbundet och otränade personer, det finns tendenser till att de som tränar har större lumendiameter. Det resultatet överensstämmer med några av de tidigare studierna, med tanke på denna studies storlek börstörre och mer omfattande studier behöver göras för säkrare resultat. / The artery in the knee, popliteal artery, can get an aneurysm, usually if the person already hasan aortic aneurysm. Vessels are affected by exercise in different ways. Various studies say that the lumen diameter in a. poplitea both increases and decreases with aerobic exercise. The purpose of this study is to see if regular aerobic exercise affects the diameter of the popliteal artery. Using ultrasound, the lumen diameter of the popliteal artery has been measured in trained and untrained women and men, in total 31 volunteers was examined. The collected data were then analysed with IBM SPSS version 27. This study confirms that men have larger lumen diameters in the popliteal artery than women. The lumen diameter of the popliteal artery is larger in its proximal and medial parts, than in the distal parts. This study has concluded thatthere is no significant difference in the popliteal artery's lumen diameter in trained and in untrained persons, however there is a tendency that trained persons have larger lumen diameters than untrained persons. That is consistent with some of the previous studies, but larger and more comprehensive studies need to be done for more accurate results.

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Bakgrund: Tetrakloretylen (TTCE) har flera humantoxikologiska och ekotoxikologiskaegenskaper, bla ökad risk för flera olika typer av cancer samt bildande av marknära ozon som bidrar till växthuseffekten. Ämnet används ofta som lösningsmedel vid analys av mineraloljor i vatten med hjälp av IR-spektrofotometri. TTCE är svårt att ersätta med andra lösningsmedel på grund av dess positiva egenskaper vid denna typ av analys. Bland annat ger TTCE inget eller ytterst lite utslag på FTIR spektrat där avläsning av alifatiska och aromatiska kolväten sker. Syfte: Syftet med rapporten är att validera ett nytt instrument samt att minimera hälsoriskerna för berörd laboratoriepersonal som arbetar med tetrakloretylen. Metod: I samband med valideringen av metoden, analys av mineraloljor i vatten medinfraröd spektrofotometri, har en riskbedömning i form av en checklista genomförtstillsammans med bland annat skyddsombud för att säkerställa en säker arbetsmiljö. Resultat: Instrumentets och metodens linjäritet klarade kravet på R2&gt;0,995. Totalt extraherbara alifatiska och aromatiska kolväten i halterna 1,0 samt 15 mg/l klarade mätosäkerheten på 15%. Data över riktigheten behöver kompleteras innan ett rättvist resultat kan erhållas. Slutsats: Valideringen av metoden LidMiljö0A.01.19 version 10, mineralolja i vatten med infraröd spektrofotometri, visar att den tidigare rapporteringsgränsen på 1,0 mg/l kommer att kvarstå. Hälsoriskerna för personalen som arbetar med analysmetoden, kan enligt denna studie minimeras till en godtagbar nivå som innebär att laboranten utsätts för minimal risk. / Background: Tetrachlorethylene (TTCE) has several human- and ecotoxicological properties, including increased risk for several types of cancer, as well as formation of ground-level ozone that contributes to the greenhouse effect. The substance is often used as solvent for the analysis of mineral oils in water by means of IR spectrophotometry. TTCE is difficult to replace with other solvents due to its positive properties in this type of analysis. Among other things, TTCE gives no or very little impact on the FTIR spectra where reading of aliphatic and aromatic hydrocarbons occurs. Purpose: The purpose of this report is to validate a new instrument and to minimize the health risks for the laboratory staff using the method. Method: In connection with validation of the method, analysis of mineral oils in water with infrared-spectrophotometry, a risk assessment in the form of a checklist has been implemented together with personnel which has an increased resonsibility of the working enviroment, to ensure a safety of the workplace. Results: The linearity of the instrument and the method met the requirement of R2&gt; 0,995. Total extractable aliphatic and aromatic hydrocarbons at levels of 1,0 and 15 mg/l achieved uncertainty of the measurement by 15%. The data regarding correctness needs to be further expanded to obtain a relaiable result.3 Conclusion: The validation of the method LidMiljö0A.01.19 version 10, mineral oil in water with infrared-spectrophotometry, shows that the previous limit of 1,0 mg/l will remain. Health risks for the personnel involved in the analysis method can, according to this study, be minimized to an acceptable level.

Around 130-150 million people are chronically infected with hepatitis C-virus today. Personal suffering and great societal costs aside, this also causes around 400 000 deaths every year due to complications. Earlier treatments with pegylated interferon and ribavirin were both ineffective and had a lot of side effects, but in recent years new treatments using direct-acting antivirals that are both more effective and has less side effects have been introduced. The downside with these are that their effectiveness depends on which HCV genotype the patient has, which requires costly and time consuming pre-treatment examinations before treatment can begin. This paper investigates clinical studies in which the effectiveness and side effects of a new combination of drugs containing sofosbuvir and velpatasvir is tested. The studies where found through PubMed, and they are what the usage of these drugs in health care is based on. The studies show that the combination of sofosbuvir and velpatasvir is effective, measured by sustained virological response, against all HCV genotypes irrespective of if the patient has a compensated or decompensated cirrhosis, or if the patient has had a previously failed treatment. The patients with decompensated cirrhosis also had an early improvement in liver function, but it is too early to say if the improvement is long lasting. The treatment also gave less side effects compared to pegylated interferon and ribavirin. The combination of sofosbuvir and velpatasvir was also shown to have a good effect amongst patients where resistance associated variants of NS5A and NS5B where found. The conclusion is that the combination of sofosbuvir and velpatasvir is an effective, but also at the moment expensive, treatment for HCV independent of genotype.

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