Ten cows were used in 4 x 4 Latin squares with an extra period for estimation of carry-over effects to study plasma amino acid responses to jugular infusion of amino acids at early, mid, and late lactation. Methionine (M), methionine + lysine (ML), and methionine + lysine + phenylalanine (MLP) in a balance with glutamic acid (C) were infused at 25% of the amino acid content of pretreatment milk via the jugular vein. Rations were formulated at 16, 15, and 14% crude protein and 15, 18, and 21% crude fiber for 30, 120, and 240 day lactational groups. Carry-over effects were present only in arterial blood at 240 days. Differences in milk production were not significant, although production increased on M, ML, and MLP at 30 days. Plasma amino acid responses of nonruminants were utilized to aid in evaluation. Arterial and venous plasma amino acid concentrations were lowest on ML at 30 days, while amino acid uptake was significantly increased by M. At 120 days plasma responses were inconclusive and non-significant. The amino acid uptake trend followed that observed at 30 days. At 240 days plasma amino acid concentrations were lowest with ML, while uptake was increased on MLP. Essential amino acids were ranked in potential orders of limitation by amino acid extraction and utilization for milk protein by the mammary gland. Order of limitation differed between calculation methods but was similar for all three stage.s of lactation. Orders of limitation were as follows: Amino acid extraction - methionine~ lysine, arginine, leucine, phenylalanine, isoleucine, threonine, valine, and histidine. Amino acid utilization - phenylalanine, threonine or histidine, valine, leucine, methionine, lysine, isoleucine, and arginine. Since there was no substantial change in order of limitation with infusion, either protein intakes were above requirements or amino acids not infused are candidates for limitation. However, among amino acids infused, the parameters observed suggested lysine or methionine at 30 days, methionine at 120 days, and no apparent choice at 240 days. / Master of Science

Two studies were designed to investigate the time course development of enlarged plasma volume and cholesterol pool size in copper (Cu)-deficient rats as well as influence of Cu deficiency on the lipid composition of lipoproteins. Rats were randomly assigned to three dietary Cu treatments (deficient, marginal, and adequate) in the Study I and two dietary Cu treatments (deficient and adequate) in Study II. Enlargement of plasma volume and cholesterol pool size were established prior to the increase in plasma cholesterol concentration. Cu concentration was decreased, whereas iron and zinc concentrations were increased in the organs of Cu-deficient and Cu-marginal rats. The plasma pool size of VLDL triglyceride was elevated 6-fold, protein and phospholipid were unaltered, and cholesterol was reduced 36%. The plasma pool size of lipid and protein components of HDL and LDL fractions were markedly elevated in Cu-deficient rats.

O Hexaclorociclohexano (HCH) é um inseticida do grupo dos organoclorados, composto por uma mistura de isômeros formados durante a síntese química, através de sucessivas adições de cloro ao benzeno. Estes isômeros podem contaminar não só o meio ambiente como também a população que tenha contato direto ou indireto com os resíduos. Nas décadas de 70 e 80, foi utilizado um produto comercial composto pelo ativo HCH comercializado como \"Hexabel®\" no controle de insetos xilófagos no Acervo Histórico de Joinville em Santa Catarina. A aplicação do inseticida foi realizada pelos próprios funcionários do Arquivo Histórico, e houve intensa manipulação dos documentos tratados durante uma mudança do prédio o presente trabalho teve como objetivo validar uma metodologia de determinação de resíduos do HCH e seus isômeros em soro sangüíneo, e analisar o grau de exposição dos funcionários e ex-funcionários do Arquivo Histórico de Joinville ao produto. Foram realizados exames sorológicos em todos os funcionários e ex - funcionários do local e também na população que sabidamente nunca tiveram contato com o ativo, denominada população controle. As análises foram realizadas após a validação do método multiresíduos que obtém todos os ativos em uma única extração e tem detecção por Cromatografia a gás com detector de captura (GC-ECD) de elétrons. Os resultados comprovaram comprovaram a eficiência do método, através de dados em conformidade com os critérios do ensaio. Os resultados obtidos no estudo da população mostraram que a quantidade de HCH no soro sangüíneo de funcionários, ex-funcionários e da população controle estão abaixo do limite de quantificação de 0,04μg dL-1 para Alfa HCH e Gama HCH \"Lindana\" e 0,08μg dL-1 para Beta e Delta HCH. / Hexachlorcyciohexane (HCH) is an organochloride insecticide formed by a mixture of chemical isomers produced during its chemical synthesis, obtained after successive inserts of chlorine atoms on benzene molecule. These isomers may contaminate both the environment and the people who had direct or indirect contact with the HCH residues. On 70\'s and 80\'s, its was used a commercial product which contained HCH on its formula and was commercialized as Hexabel®. The product was used to control the xylophage insect population on the Historical Archieve of Joinville, at Santa Catarina, Brazil. The own employees did the insecticide application and there was an intensive manipulation of the treated material during a building change. The objective of this work was to validate a methodology to quantify the HCH residues and its isomers on blood plasma and analyze the employee\'s exposure extent to the chemical. It was done serological investigation on employees, former-employees and on non-exposed people, which was classified as the control population. The analyses were done after the methodology validation. The method was capable to obtain all isomers on a unique extraction and the detection and quantification were done by gas chromatography with electron-capture detector (GC-ECD). The results obtained showed that the proposed method is accurate, and that the amount of HCH residues on blood plasma of all individuals analyzed was lower than the quantification limits established to the method, which were 0,04 μg dL-1 for the alpha- and gamma- HCH (lindana) and 0,08 μg dL-1 for the beta- and delta- HCH.

The purpose of this study was to determine if maximal aerobic exercise promotes BDNF expression in obese individuals. Plasma levels and the expression of BDNF in PBMCs were examined. 22 participants (10 obese, 12 non-obese) completed a V02max treadmill test and blood was obtained pre, post, and 1 and 2 hours into exercise recovery. Plasma and PBMCs were isolated and analyzed for BDNF via ELISA and Western blot techniques. A significant effect for time was observed for plasma BDNF (P= <0.00 1 ). Additionally, A significant group-by-time interaction was found from pre-to-RIH for BDNF expression in PBMCs (P= 0.046). Further, significant correlations were found between BMI and waist circumference (r= .91, P< 0.001), WHR (r= .51, P= 0.002) and Pre-to-RlH ratio (r=0.58, P=0.008). Young obese subject's BDNF response to maximal exercise was consistent with previous studies. Post-exercise BDNF expressed in PBMCs were significantly higher than rest suggesting immunological-neuroprotective interactions in the CNS. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2016. / FAU Electronic Theses and Dissertations Collection

Shing, Ka Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 158-188). / Abstracts in English and Chinese. / ABSTRACT --- p.i / 摘要 --- p.iv / ACKNOWLEDGEMENTS --- p.vi / TABLE OF CONTENTS --- p.vii / LIST OF TABLES --- p.x / LIST OF FIGURES --- p.xii / Chapter SECTION I: --- BACKGROUND --- p.1 / Chapter CHAPTER 1: --- CELL-FREE NUCLEIC ACIDS IN HUMAN BODILY FLUIDS --- p.2 / Chapter 1.1 --- Early studies on the presence of cell-free nucleic acids in human bodily fluids --- p.2 / Chapter 1.2 --- Circulating nucleic acids in plasma and serum --- p.2 / Chapter 1.2.1 --- Cancer Detection --- p.3 / Chapter 1.2.1.1 --- Circulating tumor-derived DNA --- p.3 / Chapter 1.2.1.2 --- Circulating tumor-derived RNA --- p.5 / Chapter 1.2.2 --- Prenatal diagnosis --- p.7 / Chapter 1.2.2.1 --- Circulating fetal DNA --- p.7 / Chapter 1.2.2.2 --- Circulating fetal messenger RNA --- p.11 / Chapter 1.2.2.3 --- Circulating placental microRNA --- p.13 / Chapter 1.3 --- Cell-free nucleic acids in urine --- p.14 / Chapter 1.3.1 --- Transrenal DNA (Tr-DNA) --- p.15 / Chapter 1.3.1.1 --- Biology of Tr-DNA --- p.15 / Chapter 1.3.1.2 --- Detection of fetal-derived Tr-DNA --- p.15 / Chapter 1.3.1.3 --- Potential problems associated with the detection of Tr-DNA --- p.16 / Chapter 1.3.2 --- Cell-free DNA in urine as released from the urinary tract --- p.17 / Chapter 1.4 --- Other bodily fluids with cell-free nucleic acids --- p.18 / Chapter 1.4.1 --- Amniotic fluid --- p.19 / Chapter 1.4.2 --- Cerebrospinal fluid (CSF) --- p.20 / Chapter 1.4.3 --- Peritoneal fluid --- p.20 / Chapter CHAPTER 2: --- MICRORNA IN HUMANS --- p.21 / Chapter 2.1 --- Introduction --- p.21 / Chapter 2.2 --- Biogenesis --- p.21 / Chapter 2.2.1 --- Transcription of microRNA genes --- p.21 / Chapter 2.2.2 --- Processing and maturation of microRNA precursors --- p.23 / Chapter 2.3 --- Mechanisms of gene regulation --- p.24 / Chapter 2.3.1 --- Cleavage of target mRNA --- p.24 / Chapter 2.3.2 --- Translational repression of mRNA --- p.25 / Chapter 2.4 --- Functional roles of microRNAs --- p.25 / Chapter 2.4.1 --- Oncogenesis --- p.25 / Chapter 2.4.2 --- Programmed cell death --- p.26 / Chapter 2.4.3 --- Cellular differentiation and development --- p.27 / Chapter 2.4.4 --- Regulation of physiological and cellular processes --- p.28 / Chapter 2.5 --- Aim of this thesis --- p.28 / Chapter SECTION II: --- MATERIALS AND METHODS --- p.30 / Chapter CHAPTER 3: --- QUANTITATIVE ANALYSIS OF CIRCULATING AND URINARY NUCLEIC ACIDS --- p.31 / Chapter 3.1 --- Preparation of samples --- p.31 / Chapter 3.1.1 --- Preparation of plasma --- p.31 / Chapter 3.1.2 --- Preparation of blood cells --- p.32 / Chapter 3.1.3 --- Preparation of placental tissue --- p.32 / Chapter 3.1.4 --- Preparation of urine and urine cell pellet --- p.32 / Chapter 3.2 --- Nucleic acid extraction --- p.33 / Chapter 3.2.1 --- "Extraction of small RNA-containing total RNA from plasma, blood cells and placental tissue" --- p.33 / Chapter 3.2.2 --- Extraction of DNA from urine --- p.37 / Chapter 3.3 --- Quantitative measurements of nucleic acids --- p.38 / Chapter 3.3.1 --- Principle of real-time quantitative PCR --- p.38 / Chapter 3.3.2 --- One-step QRT-PCR assays for mRNA quantification --- p.40 / Chapter 3.3.2.1 --- Principle --- p.40 / Chapter 3.3.2.2 --- Quantification of human placental lactogen （hPL) mRNA --- p.40 / Chapter 3.3.3 --- Two-step QRT-PCR assays for microRNA quantification --- p.45 / Chapter 3.3.3.1 --- Principle --- p.45 / Chapter 3.3.3.2 --- Advantages --- p.46 / Chapter 3.3.3.3 --- TaqMan® MicroRNA Assays --- p.47 / Chapter 3.3.4 --- QPCR assays for DNA quantification --- p.53 / Chapter 3.3.4.1 --- Principle --- p.53 / Chapter 3.3.4.2 --- Quantification of the leptin gene and the sex-determining region on Ychromosome gene --- p.53 / Chapter 3.4 --- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) --- p.57 / Chapter 3.4.1 --- Principle --- p.57 / Chapter 3.4.2 --- Zinc finger protein gene assay for determining the fractional concentration of male DNA --- p.58 / Chapter 3.5 --- Statistical analyses --- p.65 / Chapter SECTION III: --- CIRCULATING PLACENTAL MICRORNAS IN MATERNAL PLASMA AS MARKERS FOR PRENATAL DIAGNOSIS　 --- p.66 / Chapter CHAPTER 4: --- THE EXISTENCE AND QUANTITATIVE DETECTION OF CELL-FREE MICRORNAS IN PLASMA --- p.67 / Chapter 4.1 --- Introduction --- p.67 / Chapter 4.2 --- Materials and methods --- p.69 / Chapter 4.2.1 --- Sample collection --- p.69 / Chapter 4.2.2 --- Experimental design --- p.69 / Chapter 4.2.3 --- RNA extraction and quantification --- p.72 / Chapter 4.3 --- Results --- p.75 / Chapter 4.3.1 --- Validation of two-step QRT-PCR system for miRNA quantification --- p.75 / Chapter 4.3.2 --- Detection of cell-free miRNA in maternal plasma --- p.82 / Chapter 4.4 --- Discussion --- p.82 / Chapter CHAPTER 5: --- SYSTEMATIC IDENTIFICATION AND CHARACTERIZATION OF PLACENTAL MICRORNAS IN MATERNAL PLASMA --- p.86 / Chapter 5.1 --- Introduction --- p.86 / Chapter 5.2 --- Materials and methods --- p.88 / Chapter 5.2.1 --- Sample collection --- p.88 / Chapter 5.2.2 --- Experimental design --- p.88 / Chapter 5.2.3 --- RNA extraction and miRNA quantification --- p.91 / Chapter 5.3 --- Results --- p.93 / Chapter 5.3.1 --- A systematic search for placental miRNAs in maternal plasma using two-step QRT-PCR assays --- p.93 / Chapter 5.3.2 --- Detection rate and clearance kinetics of placental miRNAs in maternal plasma --- p.97 / Chapter 5.3.3 --- Effects of filtering maternal plasma on the concentration of placental miRNA and mRNA --- p.99 / Chapter 5.3.5 --- Temporal profile of placental miRNA concentrations in maternal plasma across different trimesters of pregnancies --- p.103 / Chapter 5.4 --- Discussion --- p.115 / Chapter SECTION IV: --- DETECTION OF CELL-FREE DNA IN URINE --- p.119 / Chapter CHAPTER 6: --- HEMATOPOIETIC STEM CELL TRANSPLANTATION RECIPIENTS AS A MODEL TO STUDY CELL-FREE DNA IN URINE --- p.120 / Chapter 6.1 --- Introduction --- p.120 / Chapter 6.2 --- Materials and methods --- p.123 / Chapter 6.2.1 --- Sample collection --- p.123 / Chapter 6.2.2 --- Experimental design --- p.124 / Chapter 6.2.3 --- DNA extraction and quantification --- p.125 / Chapter 6.3 --- Results --- p.128 / Chapter 6.3.1 --- Validation of the zinc finger protein gene assay --- p.128 / Chapter 6.3.2 --- Fractional concentration of male DNA in blood cells and plasma of sex-mismatched HSCT patients --- p.129 / Chapter 6.3.3 --- Fractional concentration of male DNA in the urine and the urine cell pellets of sex-mismatched HSCT patients --- p.131 / Chapter 6.3.4 --- Size distribution of cell-free DNA in peripheral blood and urine samples of sex-mismatched HSCT patients --- p.132 / Amplicon size --- p.138 / Chapter 6.4 --- Discussion --- p.143 / Chapter SECTION V: --- CONCLUDING REMARKS --- p.147 / Chapter CHAPTER 7: --- CONCLUSION AND FUTURE PERSPECTIVES --- p.148 / Chapter 7.1 --- Circulating miRNA is a valuable resource for molecular analysis --- p.148 / Chapter 7.2 --- The presence of donor-derived DNA in the urine of HSCT recipients --- p.150 / Chapter 7.3 --- Prospects for future work --- p.152 / APPENDIX 1 --- p.154 / REFERENCES --- p.158

Thesis (Ph.D)--Chemistry and Biochemistry, Georgia Institute of Technology, 2007. / Committee Chair: Sheldon May; Committee Members: Nicholas Hud, Stanley Pollock, James Powers, and Gerald Pullman. Part of the SMARTech Electronic Thesis and Dissertation Collection.

Šiame darbe nagrinėjami kietazės ekstrakcijos ir skysčių – skysčių ekstrakcijos metodai, bei lyginami jų efektyvumai ekstrahuojant kodeiną iš kraujo plazmos ir šlapimo. Ekstrakcijų efektyvumai buvo nustatinėjami UV spektrometrijos būdu. Darbo tikslas – nustatyti optimalias mėginio ruošimo toksikologinėje analizėje metodikas, tinkamas kodeino išskyrimui iš biologinių terpių – kraujo plazmos, šlapimo, bei palyginti šių metodikų efektyvumą. įvertinus gautus duomenis, galima teigti, kad ekstrahuojant kodeiną iš šlapimo, skysčių – skysčių ekstrakcijos ir kietafazės ekstrakcijos efektyvumai buvo panašūs. Ekstrahuojant kodeiną iš kraujo plazmos – efektyvesnė pasirodė kietafzė ekstrkcija. / In this master’s thesis are examined solid phase extraction and liquid - liquid extraction methods. Codeine has been extracted by using these methotds, efficiencies of extraction methods were determined by UV spectrophotometry. The aim of this this master’s thesis is to determine the optimal sample preparation methodologies for toxicological analysis, codeine proper isolation of biological media - plasma, urine, and to compare the effectiveness of these methods. After assessment of the data, it can be said that the extraction of codeine from urine, efficiencies were similar extracting by using liquid - liquid extraction and solid phase extraction. Codeine is more efficiently extracted from blood plasma by solid phase extraction method than liquid - liquid extraction method.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2004. / Härtill 7 uppsatser.

Plasma protein shave many important functions in the organism. Gamma globulins are carriers of immunoglobulins which play animportant role in the immune response. Their contentis primarily given by the health burden of the organism. The aimofthe study was to determine the individual protein fractions in the blood plasma of ewes and lambs, comparemutual relations between total plasma proteins and thein variol factions and assess the concentrations of individual plasma proteins, mainly globulin, in connection with the aktivity of the thyroidgland, physiological state, and increments. The sampling was carried out in the spring (25.3.) and the autumn (14.10) 2013. The individual protein fractions were determined from the serum by the means of electrophoresis. The results show that the concentration of proteins in the blood plasma of bothewes and ewe lambs and ram lambs was high due to haemoconcentration, heat stress during sampling, grazing young green forage, comprising a large number of protein aceous substances, and increasing demands on energy for milk production, especially in the early stagesoflactation. Theconcentrationofproteins in theblood plasma oflambspertains to thegrowthproduction. It establishes a correlation between the concentration of plasma proteins and the aktivity of the thyroidgland. It also confirmes higher concentrations of plasma proteins of lambs correlating to thein higher daily gain. Due to the high concentration of total protein in the plasma, the level of its fractions was high as well. After the conversion to a percentage, the level of - globulins in blood plasma in all categories was within normal limits in the range of 14-27%. The concentration of - globulins in blood plasma increased in relation to the parasitological findings coccidia of the genus Eimeria and gastrointestinal nematodes. Relations between the kontent of plasma proteins in the blood of the lambs and ewe sobserved were in most casespositive. There was a strong dependence between the total protein and globulins. Even among other fractions of plasma proteins and globulins correlation coefficient was almou always positive.

SOUZA, Francisco Thiago Correia de. Validação e determinação de carbofurano e carbaril em plasma sanguíneo de agricultores do perímetro irrigado do baixo Jaguaribe-CE por MEFS-HS/CG-EM. 2014. 89 f. Dissertação (Mestrado em química)- Universidade Federal do Ceará, Fortaleza-CE, 2014. / Submitted by Elineudson Ribeiro (elineudsonr@gmail.com) on 2016-10-11T14:37:03Z No. of bitstreams: 1 2014_dis_ftcsouza.pdf: 1510094 bytes, checksum: 558971bf2d922838752f45e145b0532f (MD5) / Approved for entry into archive by Jairo Viana (jairo@ufc.br) on 2016-10-11T18:25:16Z (GMT) No. of bitstreams: 1 2014_dis_ftcsouza.pdf: 1510094 bytes, checksum: 558971bf2d922838752f45e145b0532f (MD5) / Made available in DSpace on 2016-10-11T18:25:16Z (GMT). No. of bitstreams: 1 2014_dis_ftcsouza.pdf: 1510094 bytes, checksum: 558971bf2d922838752f45e145b0532f (MD5) Previous issue date: 2014 / The presence of pesticides is increasing concern in Brazil since 2007 to 2012, the volume of pesticides (considering only the active ingredient) applied to the crops grew 14 %. The uncontrolled use of these compounds has offered risks to workers and residents of the areas of agribusiness. A survey conducted by a group of Federal University of Ceará, showed that in 23 samples of water from the irrigated perimeter region of low Jaguaribe, collected at different locations and analyzed by liquid chromatography, all showed the presence of some pesticides, highlighting the carbaryl and carbofuran as the most detected. One study found chromosomal abnormalities in bone marrow cells of individuals exposed to pesticides in the region, which reinforces the need to develop bioanalytical methods able to detect pesticides in biological samples, for which exposure can be monitored by competent authorities. Based on that a method was developed for analysis of carbaryl and carbofuran, using a gas chromatograph equipped with mass detector, it was monitored the analytical signal of pesticides and their respective products of thermal degradation, degradation that occurred gun chromatograph. A study to determine the appropriate line speed for the method was carried out with the construction of the Van Deemter diagram, being determined as great as the speed of 50.5 cm s-1. Sample preparation was carried out with the precipitation of the plasma proteins microextraction followed by solid phase in the headspace (HS-SPME) of the supernatant; to extract optimization was performed factorial design with central component 22 in three fibers having different characteristics. The Polydimethylsiloxane/Divinylbenzene fiber (PDMS/DVB) of 65 μm showed a higher affinity for analytes and determined as optimum conditions the time of 40 min. extraction with NaCl 30 % w/v of ionic strength and temperature of 110 °C; pH 5.5, stirring speed of 1000 rpm and 40 ml vial were kept constant. A method for quantitative determination has been validated only for carbaryl, making monitored by signal 1-naphthol. The % 1-naphthol produced by thermal degradation of carbaryl in the conditions of SPME and GC-MS were respectively determined by NMR 8 % and 94 % as determined by the generated signal detector. The selectivity was evaluated in blood plasma matrix for normal, hemolyzed, and lipemic samples, the highly selective method. The linear response range was 12-180 μg L-1; using the matrix method of superposition using the substitute pattern as pirimicarb, the linearity of calibration curve was determined by the value of the correlation coefficient (R = 0.999), being conducted further study of the linearity. The values of detection and limit of quantification were 3.0 and 12.0 μg L-1, demonstrating that small concentrations can be analyzed by the method; the precision and accuracy intra and inter race were all within the recommended resolution by the National Sanitary Surveillance Agency No. 27/2012, less than 15% for medium to high concentrations and lower than 20 % for low concentrations. Samples of 10 workers in the region were analyzed, but was not detected presence of carbaryl and carbofuran in plasma of these individuals. / A presença dos agrotóxicos é cada vez mais preocupante no Brasil visto que entre 2007 e 2012, o volume de defensivos (considerando apenas o princípio ativo) aplicado nas lavouras cresceu 14 %. O uso descontrolado desses compostos tem oferecido riscos a trabalhadores e moradores das regiões do agronegócio. Uma pesquisa realizada por um grupo da Universidade Federal do Ceará, mostrou que em 23 amostras de água da região do perímetro irrigado do baixo Jaguaribe, coletadas em diferentes localidades e analisadas por cromatografia líquida, todas apresentaram a presença de algum agrotóxico, destacando-se o carbaril e o carbofurano como os mais detectados. Um estudo constatou anormalidades cromossômicas em células da medula óssea de indivíduos expostos a pesticidas na região, o que reforça a necessidade de desenvolvimento de métodos bioanalíticos capazes de detectar pesticidas em amostras biológicas, para que essa exposição possa ser monitorada pelas autoridades competentes. Baseado nisso um método foi desenvolvido para análise de carbaril e carbofurano, usando um cromatógrafo a gás equipado com detector de massas, em que foi monitorado o sinal analítico do agrotóxico e de seus respectivos produtos de degradação térmica, degradação essa ocorrida injetor do cromatógrafo. Um estudo para determinação da melhor velocidade linear para o método foi realizado com a construção do diagrama de Van Deemter, sendo determinada como ótima, a velocidade de 50,5 cm s-1. O preparo da amostra foi realizado com a precipitação das proteínas do plasma seguida de microextração em fase sólida no modo headspace (MEFS-HS) do sobrenadante; para otimização da extração foi realizado planejamento fatorial 22 com componente central, em 3 fibras com características diferentes. A fibra Polidimetilsiloxano/Divinilbenzeno (PDMS/DVB) de 65µm mostrou uma maior afinidade com os analitos, sendo determinada como condições ótimas o tempo de 40 min. de extração com NaCl 30 % p/v de força iônica e 110 °C de temperatura; o pH 5,5, agitação de 1000 rpm e vial de 40 mL foram mantidos constantes. Foi validado um método para determinação quantitativa apenas para o carbaril, sendo esse monitorado pelo sinal do 1-naftol. A % de 1-naftol produzido pela degradação térmica do carbaril, nas condições de MEFS e no CG-EM, foram respectivamente de 8% determinado por RMN e 94 % determinado pelo sinal gerado no detector EM. A seletividade foi avaliada na matriz de plasma sanguíneo para amostras normais, lipêmica e hemolisada, sendo o método bem seletivo. A faixa linear de trabalho foi de 12 a 180 µg L-1; utilizando o método de superposição da matriz com a utilização do pirimicarbe como padrão substituto, a linearidade da curva de calibração foi determinada pelo valor do coeficiente de correlação (R = 0,999), sendo realizado um estudo mais aprofundado da linealidade. Os valores do limite de detecção e quantificação foram de 3,0 e 12,0 µg L-1, o que demonstra que pequenas concentrações podem ser analisadas pelo método; a precisão e exatidão intra e inter corrida foram todos dentro do recomendado pela resolução da Agência Nacional de Vigilância Sanitária Brasileira nº 27/2012, menores que 15 % para concentrações médias e altas e, menores que 20 % para baixas concentrações. Amostras de 10 trabalhadores da região foram analisadas, porém não foi detectada presença de carbaril e carbofurano no plasma desses indivíduos.