by Lee, Chun-Sing. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 98-105). / Abstracts in English and Chinese. / Acknowledgements --- p.2 / Abstract --- p.3 / Table of Contents --- p.7 / List of Figures --- p.10 / List of Tables --- p.13 / Chapter Chapter 1 --- Introduction --- p.14 / Chapter 1.1. --- Ion implantation --- p.14 / Chapter 1.2. --- Scope of the thesis --- p.15 / Chapter Chapter 2 --- Background Theory --- p.17 / Chapter 2.1. --- Ion stopping --- p.17 / Chapter 2.2. --- The energy-loss process --- p.17 / Chapter 2.3. --- Kinematics of binary elastic collision --- p.20 / Chapter 2.4. --- Nuclear and electronic stopping --- p.21 / Chapter 2.5. --- Radiation Damage --- p.22 / Chapter 2.6. --- Spikes --- p.24 / Chapter 2.7. --- Topography of ion bombarded surface --- p.26 / Chapter Chapter 3 --- Equipment Reviews --- p.31 / Chapter 3.1. --- Metal Vapour Vacuum Arc Ion Source Implanter --- p.31 / Chapter 3.2. --- Atomic Force Microscopy --- p.34 / Chapter 3.3. --- Rutherford Backscattering Spectrometry --- p.37 / Chapter 3.4. --- X-ray Diffraction --- p.40 / Chapter Chapter 4 --- Study of Ion Beam Implanted Germanium by Atomic Force Microscopy and Rutherford Backscattering Spectrometry --- p.43 / Chapter 4.1. --- Introduction --- p.43 / Chapter 4.2. --- Experiments --- p.45 / Chapter 4.3. --- Results and discussion --- p.47 / Chapter 4.3.1. --- AFM --- p.47 / Chapter 4.3.2. --- RBS and ion channeling --- p.64 / Chapter 4.4. --- Conclusions --- p.71 / Chapter Chapter 5 --- Ion Beam Synthesised Cobalt Germanide Alloy by Metal Vapour Vacuum Arc Implantation --- p.73 / Chapter 5.1. --- Introduction --- p.73 / Chapter 5.2. --- Experiments --- p.74 / Chapter 5.3. --- Results and discussion --- p.74 / Chapter 5.3.1. --- XRD --- p.74 / Chapter 5.3.2. --- AFM --- p.78 / Chapter 5.3.3. --- RBS and ion channeling --- p.82 / Chapter 5.4. --- Conclusions --- p.87 / Chapter Chapter 6 --- Tip Artifacts in Atomic Force Microscope Imaging of Ion Bombarded Nanostructures on Germanium Surfaces --- p.89 / Chapter 6.1. --- Introduction --- p.89 / Chapter 6.2. --- Experiments --- p.90 / Chapter 6.3. --- Results and discussion --- p.90 / Chapter 6.4. --- Conclusions --- p.95 / Chapter Chapter 7 --- Conclusions --- p.96 / Bibliography --- p.98 / Publications --- p.105

"May 2002." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Yuen Yung Hui. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2002. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese. / Yuen Yung Hui.

This thesis is for a Master of Fine Arts in Sculpture.

Cluster ToF-SIMS (time-of-flight secondary ion mass spectrometry) operated in the event-by-event bombardment/detection mode has been applied to: 1) evaluate and screen the manufacturing quality of step-wise prepared micropatterned biointerfaces; 2) quantify the binding density of Au nanoparticles (AuNPs)-antiCD4 conjugates selectively attached on the cell surface; 3) elucidate the biological interaction of proteins and molecules by quantifying the fractional coverage of immobilized biomolecules; 4) enhance the accuracy of secondary ion identification of specific molecules. Briefly, our method consists of recording the secondary ions, SIs, individually emitted from a single projectile impact (C60 1,2+, Au400 +4). From the set of individual mass data, we select events where a specific SI was detected. The selected records reveal the SIs co-ejected from the nanovolume impacted by an individual cluster projectile from an emission area of 10-20 nm in diameter and an emission depth of 5-10 nm. The approach for quantifying the number of AuNPs or that of specific nanodomains is via the concept of the fractional coverage. The latter is the ratio of the effective number of projectile impacts on a specified sampling area (Ne) to the total number of impacts (No). The methodology has been validated with the determination of the number of antibody-AuNP conjugates on a cell, i.e. the number of disease related antigens on a cell via their specific binding sites with the AuNP-labeled antibodies. The number of AuNP-antibodies measured, ~42000 per cell, is in good agreement with literature results. The fractional coverage concept was also used to quantify several variants of biointerfaces. An example is the quantification of biotin and avidin immobilization as a function of the composition of silane substrates. The data collected in the event-by-event bombardment/detection mode expands the scope and quality of analytical information. One can identify SIs co-emitted with two specified SIs (double coincidence mass spectrometry) to inspect a specific stratum of a biointerface. A further refinement is the selection of events meeting a double coincidence emission condition. This mode enables the identification of nano-object of a few nm in size, which eliminates (anticoincidence) interferences from substrates.

The objective of this study was to optimize tissue culture, transformation and regeneration parameters of mature embryo based culture of Triticum durum cv. Mirzabey 2000 and Triticum aestivum cv. Y&uuml / regir 89. The effects of auxin type of hormone at different concentrations and dark incubation periods on regeneration capacity were evaluated. Two different hormone types 2,4- dichlorophenoxyacetic acid and picloram were used at three different concentrations 2, 4 and 8 mg/l. Mature embryo derived calli were incubated in 6 different induction media at dark for 4 and 6 weeks for initiation of primary callus induction. After dark incubation periods, average callus fresh weight and primary callus induction rate were determined. The primary callus induction rates for 4 weeks and 6 weeks old dark adapted Mirzabey calli incubated was found to be 91 % and 93.25 % respectively. Y&uuml / regir primary callus induction rate was 92.5 % for 6 weeks old calli in 6W2D medium and 86.75 % for 4 weeks old calli in 4W8P medium. The primary calli were transferred to embryogenic callus induction medium. The embryogenic callus formation was 94.88 in 6W2D medium for Mirzabey cultivar. The necrosis was observed at high concentration of 2,4-D for both of cultivars. After embryogenic callus induction, embryogenic calli were transferred into hormone free regeneration medium. The maximum regeneration rate (62.31 %) and culture efficiency (44.13 %) were observed in 4W2D medium for Mirzabey. However, the low regeneration rate was observed for Y&uuml / regir (5 %) in 6W2D medium. The transformation studies were performed by using Obitek Biolab Gene Transfer System. The old and the modified loading units were used for optimization of bombardment pressure and distance for mature embryo based calli transformation. After bombardment of pAHC25 coated gold particles, histochemical GUS assay was performed and blue spots were counted. The transformation efficiency increased to 0.65 fold for 30 bar bombardment pressure and 5.5 fold for 35 bar bombardment by the modified loading unit. The modified loading unit could be used for further transformation studies.

Drift time ion mobility spectrometry (DTIMS) is a rapid post ionization gas-phase separation technique that distinguishes between compounds based on their differences in reduced mass, charge and collisional cross-section while under a weak, time-invariant electric field. Standalone DTIMS is currently employed throughout the world for the detection of explosives, drugs and chemical-warfare agents. The coupling of IMS to MS (IM-MS) has enabled the performance of time-nested multidimensional separations with high sample throughput and enhanced peak capacity, allowing for the separation of ions not only based on their mass/charge (m/z) ratios, but also their shape. This allows for the elucidation of valuable structural information that can be utilized for determining gas phase ion conformation and differentiation between closely related ionic species. Over the past decade, these advances have transformed IM-MS applications and instrumental designs into one of the most rapidly growing areas of mass spectrometry. The work presented in this thesis is aimed at the development and subsequent characterization of a novel high-resolution resistive-glass atmospheric pressure DTIMS, and the application of this prototype DTIMS to the detection of environmentally relevant compounds. A review of the different types of ion mobility spectrometers, their principles of operation, and the advantages and disadvantages of each type are presented in Chapter 1. Chapter 2 describes the design and development of our prototype resistive glass DTIMS. A detailed description of the IMS hardware, including the ion sources, custom-built control computer, pulsing electronics, data acquisition system, and the timing schemes developed to operate the instrument in standalone DTIMS, multiplexed DTIMS, and IM-MS mode, are presented. Chapter 3 presents an initial characterization of the performance of a prototype resistive glass DTIMS under a wide range of instrumental parameters and also characterizes the radial ion distribution of the ions in the drift region of the spectrometer. Chapter 4 addresses the lack of sensitivity in DTIMS and explores ion trapping and multiplexing methods, introduces the principles of multiplexing and describes an extended multiplexing approach that encompasses arbitrary binary ion injection waveforms with variable duty cycles. Chapter 5 presents a detailed theoretical and experimental study of the separation power of our DTIMS and presents an evaluation of the field homogeneity and the performance of the ion gate.

The work described in this thesis is an analysis of the Plasma Source Ion Implantation (PSII) process. A metal target is placed within a plasma, and pulsed to a high negative potential (10 - 50 kV). The electrons in the plasma close to the target are then repelled very rapidly, leaving an area of uniform positive charge. This causes an electric field to be set up between the plasma and the metal target. The ions close to the target are then accelerated towards the target by the electric field. The ions reach the target at high velocities, and implant deeply into the metal (-5 x 10-8 m), and form nitrides, which pin dislocations within the metal's atomic structure. The strength of the metal is therefore increased, and other properties such as the corrosion resistance of the metal are also improved. Metals that have undergone the PSII process have widely diverse applications. For example, in the motor industry, ion implanted metal punches last much longer than nitrided punches, while in the medical industry ion implanted metals are used for artificial limbs. A combination of a number of different analytic, numerical and simulation models are used to describe the PSII process, including the plasma behaviour and final nitrogen implantation profile in the metal target after the application of the voltage pulse. In all cases, a specific attempt has been made to realistically describe as closely as possible, the actual experimental arrangement at the University of Natal. For example: a waveform with a fast rise time, short plateau and exponential decay was used; the nitrogen plasma was more realistically described by a two species fluid to account for the measured N+, N; mix; and finally, the actual atomic composition for 304 stainless steel was used in the TAMIX particle simulation. This work thus models the whole PSII process, and could form the basis of future studies for the optimisation of the process. / Thesis (M.Sc.)-University of Natal, 1993.

Graphene is a promising material for many technological applications. To realize these applications, new fabrication techniques that allow precise control of the physical properties, as well as large scale integration between single devices are needed. In this work, a series of studies are performed in order to develop graphene based devices. First, using MD simulations we study the effects of irradiating graphene with a carbon ion atom at several positions and energies from 0.1 eV to 100 keV. The simulations show four types of processes adsorption, reflection, transmission, and vacancy formation. At energies below 10 eV the dominant process is reflection, between 10 and 100 eV is adsorption, and between 100 eV and 100 keV the dominant process is transmission. Vacancy formation is a low rate process that takes place at energies above 30 eV. Three types of defects were found: adatom, single vacancy, and 5-8-5 defect formed from a double vacancy defect. Also a bottom-up fabrication method is studied, in this method, the controlled folding of graphene structures, driven by molecular interactions with water nanodroplets, is analyzed considering the interactions with substrates such as SiO2, HMDS and IPA on SiO2. When the graphene is supported on SiO2, the attraction between graphene and the substrate prevents graphene from folding but if the substrate has HMDS or IPA, the interaction between graphene and the substrate is weak, and depending on the geometry of the graphene structure, folding is possible. Finally, to evaluate the characteristics of graphene based devices, we model the vibrational bending modes of graphene ribbons with different dimensions. The resonant frequencies of the ribbons and relations between the size of the ribbon and their resonant frequencies are calculated. The interaction of a graphene vibronic device with water and IPA molecules are simulated and demonstrate that this device can be used as a sensitive vibronic molecular sensor that is able to distinguish the chemical nature of the detected molecule. Also, the electrical properties of the graphene vibronic with armchair and zigzag border are calculated; the latter has the potential to generate THz electrical signals as demonstrated in this work.

The inert gas ion bombardment of solid surfaces has found many uses in the field of analytical chemistry. In one method of spectrochemical analysis, inert gas ion bombardment in a glow discharge is used to produce an atomic vapor, representative of the sample bulk, which is analyzed using atomic absorption techniques. Gas jets directed at the sample surface during the discharge increase the removal rate of sample material from the surface. Such bombardment of solid surfaces results in changes to the surface which are visually evident in the formation of craters and surface deposits. This thesis was designed to gain a better understanding of the changes in a brass surface caused by jet-enhanced sputtering. Electron spectroscopy for chemical analysis (ESCA) is used to study selected regions of the surface. Changes in chemical composition were observed as successive atomic layers were removed from the sample. The results of this investigation indicate that the effects of ion bombardment can be explained in terms of the selective sputtering of one bulk component over another. The surfaces of the sputtered craters were found to be depleted of the higher sputtering yield component, Zn, when compared to the bulk composition. In a deposit, the component with the highest sputtering yield was found to be more concentrated in layers closest to the bulk material. The component with the lowest sputtering yield was found at relatively higher concentrations nearest the deposit surface. The component with the higher sputtering yield is selectively sputtered first during the glow discharge, and consequently is deposited with a higher concentration closest to the bulk, the converse being true for the lowest sputtering yield component. / Graduation date: 1993