Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Bibliography: leaves 157-167.

Thesis (MScAgric)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: In order to study the dormancy of apple buds in conditions of inadequate chilling a number of trails were done during 2000 and 2001. Year-old, unbranched shoots of 'Royal Gala', 'Braeburn', 'Cripps' Pink' and 'Granny Smith' apple were harvested randomly from bearing commercial orchards in the Koue Bokkeveld [33°S, 945m, ca.1300 Utah model chili units (CU)) and Elgin (34 "S, 305m, ca.750CU) regions of the Western Cape, South Africa, respectively. Shoots were chilled at 5-rC. Two replicate bundles were removed from the cold room, prepared and forced at 25°C with continuous illumination until budburst had occurred on at least 25% of the shoots per bundle. The change in the rate of budburst over time was calculated for each orchard and to this response; either a linear or a quadratic function was fitted. Poorly correlated variables were selected that best describe these functions. Using these variables, the orchards were separated into cluster groupings that represented a dormancy pattern. The first split separated the lower chilling requirement cultivars from the higher cultivars. The second and third split separated the orchards according to area differences. The clusters representing the warmer area orchards initially entered deeper into dormancy before exiting. The clusters for the colder area immediately had an increased budburst rate. This data confirm that the chilling requirement includes a period of dormancy induction. An important genotype and environment interaction, other than cold unit accumulation, was observed that could be responsible for terminating bud dormancy. Terminal apple buds from 'Royal Gala' Braeburn', 'Cripps' Pink' and 'Granny Smith' apples were cut from orchards in the Koue Bokkeveld and Elgin regions of the Western Cape, South Africa. Buds were harvested every two weeks during the dormant period. The buds were cut in half and leaf scales removed before the water potential were measured. Fresh and dry weights of the buds were also determined. The data presented confirms the changes in availability of free water in dormant buds and that it could be measured in this way. A definite influence of temperature was illustrated. The water potential from buds in a cold production area (Koue Bokkeveld) behaved more "normally" - water is in a bound form during most of the winter and change to an available form later in winter - whereas buds from a warmer production area did not change much in water potential or content. In the trial, two-year-old proleptic-branched shoots, ca. 500mm long, were selected from a 'Royal Gala' orchard in the Koue Bokkeveld region in the Western Cape, South Africa. During the dormancy period of 2000, shoots received two cold treatments; chilling in a cold room at 5-7°C and the natural chilling received in the field. In 2001, the trail was repeated, but only with the field chilling. The shoots received five dormant pruning treatments: control (not pruned), pruning back to the fourth lateral (heading) before or after chilling; and removal of the 2nd and 3rd laterals (thinning) before or after chilling. After pruning and chilling treatments, the shoots were removed from the orchard or cold room every two weeks and forced in a growth chamber. The rate of bud burst (1/days to 50% bud burst) was calculated for the terminal buds of the lateral shoots. Laterals were categorisation according to position: the terminal extension shoot, the 4th lateral, and all other laterals were pooled. Removing distal tissue by pruning (heading more than thinning) promoted bud burst on laterals. Pruning before chilling was more effective than after chilling. The correlative phenomena that inhibit bud burst on proximal shoots within two-yearold branches were manipulated by pruning. The branching response of one-year-old unbranched shoots, 0.5m long, from 'Royal Gala' and 'Cripps' Pink' apple and 'Rosemary' pear were studied after physical manipulation treatments. Shoots for treatment a to d were re-orientated from either a horizontal or vertical position or left in the original position as control, treatment e to h involved the same re-orientation of shoots and were headed. The amount of growth (in mm) from each node was recorded as well as the position from the terminal bud. The 'Cripps' Pink' had a definite shift in the acrotonic branching pattern (for headed and unheaded), towards a more basitonic response. The reduced effect on 'Royal Gala' and 'Rosemary' suggest a difference in genotype response to the treatments as well as time of treatment. / AFRIKAANSE OPSOMMING: Ter wille van die navorsing oor die invloed van gebrekige koue op dormansie van apple knoppe en die gepaardgaande probleme is 'n reeks proewe gedoen gedurende 2000 en 2001. Jaar oue onvertakte lote van 'Royal Gala', 'Braeburn' , 'Cripps' Pink' en 'Granny Smith' appels is ewekansig geoes vanaf komersieële boorde in die Koue Bokkeveld [33°S, 945m, ca.1300 Utah koue eenhede (CU)] en Elgin (34°S, 305m, ca.750CU) omgewings van die Wes Kaap , Suid Afrika. Die lote is daarna verkoel gehou by 5- 7°C. Lote is elke twee weke vanuit die koue kamer geneem en geforseer met 25°C en deurlopend belig. Die aantal knoppe wat groenpunt bereik het is genoteer totdat 25% van die lote begin bot. Die verandering oor tyd vir elke boord is bereken en 'n liniëre of kwadratiese funksie is daarop gepas. Swak gekorreleerde waardes is gekies wat die funksies die beste beskryf. D.m.v hierdie die waardes is die boorde in groepe ingedeel wat 'n dormansie patroon verteenwoordig. Na die eerste vedeeling is die hoë en lae koue behoefte kultivars geskei. In opvolgende verdelings is die boorde verder in die twee areas geskei met elk 'n spesifieke dormansie patroon. Die groepe wat die warmer area se boorde bevat het aanvanklik dieper in dormansie in beweeg voor dit 'n styging in groei potensiaal getoon het. Die groepe vanaf die kouer produksie area het onmiddelik 'n verhooging in bot tempo getoon. Die data bevestig dat 'n koue behoefte 'n periode van dormansie induksie insluit. 'n Belangrike kultivaromgewing- interaksie, ten spyte van koue eenhede akkumulasie, is waargeneem wat verantwoordlik kan wees vir beëindiging van dormansie. Om die verandering van water status in dormante appel knoppe te bestudeer, was die volgende proef uitgevoer. Terminale apple knoppe van dieselfde vier kultivars en vanaf die selfde twee areas in die Wes Kaap as bogenoemde, is elke tweede week gedurende die winter 2001 gesny vanaf komersieële boorde. Daarna is die knoppe middel deur gesny en die skudblare verwyder voor die vars massa gemeet en daarna die waterpotensiaal bepaal is. Die data het bevestig dat daar veranderings in beskikbaarheid van vry water in dormante knoppe gedurende dormansie plaasvind. 'n Definitiewe invloed van temperatuur op waterpotensiaal is geïllustreer. Die waterpotensiaal van knoppe in die kouer produksie area toon 'n meer normale respons (gedurende die winter is die water in 'n gebonde vorm, wat later in die winter na vry water verander). Daar teenoor is daar in die knoppe van die warm produksie area nie veel verandering in waterpotensiaal of water massa getoon nie. Gedurende die winter van 2000 is twee jaar oue proleptiese vertakte 'Royal Gala' lote, ongeveer 500mm lank, gekies vanaf 'n boord in die Koue Bokkeveld. Die lote is verdeel en het twee koue behandelings ontvang. Koue kamer by 5-T'C en natuurlike koue in die boord. In 2001 is net die natuurlike koue behandeling herhaal. Daar is vyf dormante snoei behandeling op die lote gedoen; kontrole (geen), snoei terug tot die vierde lateraal voor en na die koue; verwydering van die 2de en 3de laterale voor en na koue. Na koue en snoei is die lote verwyder en in die groeikamer geforseer by 25°C en met konstante illuminasie. Die tempo waarteen die laterale gebot het is bereken (1\dae to 50% groen punt) waarna die laterale in klasse gedeel is; terminale knop, die vierde lateraal en die res van die laterale saam. Verwydering van distale weefsel d.m.V. snoei verhoog die tempo waarteen laterale knoppe groenpunt bereik. Snoei voor die koue behandeling was meer effektief as daarna. Die korrelatiewe fenomeen wat bot inhibeer van proksimale lote kan gemanipuleer word in die twee jaar apple loot. Die vertakkings gedrag van een jaar oue onvertakte 'Royal Gala', 'Cripps' Pink' apple en 'Rosemary' peer lote, 0.5m lank, is ondersoek na fisiese manipulasies gedoen is gedurende mid winter 2000. Vir die eerste vier behandelings (a,b,c,d) is die lote in 'n horisontale of vertikale possisie gelos as kontrole, of horisontale of vertikale gebuig en daar gehou d.m.v. binddraad. Behandelings e tot h het dieselfde behels maar die lote is ook nog in die helfte deur gesny (getop). Die hoeveelheid groei in mm vir elke node is aangeteken asook die posisie vanaf die terminale knop. Die 'Cripps' Pink' het 'n definitiewe veskuiwing vanaf 'n akrotoniese na 'n basitoniese vertakkingspatroon getoon. Die reaksies op die manipulasies of die gebrek daaraan ('Royal Gala' en 'Rosemary' ) kan 'n aanduiding wees dat genotipes verskillend reageer op die behandelings asook op die tyd wat dit gedoen was.

Orientador: Elza Guimarães / Resumo: A variação em atributos de proteção, principalmente os foliares, pode ter importante papel no desempenho das espécies ao longo de um gradiente de condições ambientais ou em hábitats heterogêneos. O domínio Cerrado está inserido em um gradiente climático e suas formações savânicas estão submetidas a queimadas recorrentes. Neste trabalho nós caracterizamos atributos de proteção às gemas em três espécies lenhosas, coocorrentes em fitofisionomias savânicas ao longo do gradiente latitudinal N-S do Cerrado e investigamos a ocorrência de fogo em uma escala espácio-temporal. Caracterizamos a posição das gemas nas plantas, morfologia e anatomia e indicadores de flamabilidade das mesmas. De modo geral, as plantas de diferentes localidades apresentaram diferenças quanto a altura das gemas na planta em relação ao nível do solo, sendo que plantas mais altas foram observadas nas latitudes menores e as mais baixas nas latitudes maiores, provavelmente devido a maior produtividade associada à proximidade da linha do Equador. Primórdios com maior área ocupada por idioblastos fenólicos foram observados nas duas localidades mais próximas à linha do Equador, possivelmente devido a alta intensidade de radiação. Além disso, registramos distintos regimes de queimada nas cinco localidades nos últimos 15 anos. Considerando a ampla distribuição geográfica das três espécies deste estudo, podemos concluir que suas gemas axilares toleram certa variação nos fatores climáticos como a amplitude de temperatur... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The variation in protection traits, especially the foliar ones, can play an important role in species performance along a gradient of environmental conditions or in heterogeneous habitats. The Cerrado domain is inserted in a climatic gradient and its savannah formations are under recurrent burning. In this study, we characterized bud protection traits of three woody species that co-ocurr in savannah phytophysiognomys along the Cerrado latitudinal gradient and we investigated fire occurrence in a spatio-temporal scale. We characterized bud position in each plant species, its morphology, anatomy and flammability indicators. In general, plants of different locations showed differences in bud height in relation to soil level. Taller plants were observed at lower latitudes and the smallest ones at higher latitudes, probably due to the higher productivity associated to the proximity to Equator line. Primordia with a larger area occupied by phenolic idioblasts were observed in the two locations nearest to the Equator line, possibly due to high radiation intensity. Besides that, we registered different fire regimes at the five locations in the last 15 years. Considering the wide distribution of the three species of this study, we can conclude that their axillary buds tolerate some variation in climatic factors like temperature, solar radiation and pluviosity amplitude. Besides that, this species are capable of persisting in environments under different fire frequency. Additionally, b... (Complete abstract click electronic access below) / Mestre

D.Sc. / Please refer to full text to view abstract

The morphological development of Elberta peach fruit buds was studied in relation to their cold hardiness. Morphological development of peach fruit buds was observed beginning with bud differentiation in mid-summer. Flower initiation was essentially complete by September. Growth was slow in the winter months but increased rapidly as anthesis approached. Photomicrographs were taken to substantiate the discussion . Cold hardiness determinations were made from December until anthesis in April. The hardiness level was greatest during December and remained at a relatively high level until rest ended. Hardiness decreased thereafter. Major losses of hardiness occurred just prior to anthesis.

Disturbance is an important phenomenon affecting plant lives and shaping plant strategies in disturbed habitats. A variety of ecological concepts on individual plant response to injury has been proposed for specific natural ecosystems or growth forms. In central Europe, man-made habitats are often cases of disturbed places, so the aim of the thesis was to apply four chosen concepts on them. The thesis is composed of four original studies performed in two model ecosystems: recurrently disturbed ruderal place and regularly mown central European meadow. The occupying of the aboveground space after a disturbance event was studied here either as renovation of biomass or architecture. Regenerative strategies in herbs occupying disturbed habitats were described and confronted with concepts proposed originally for ecosystems subjected to natural disturbance regimes.

Thesis (MSc)--Stellenbosch University, 2002. / ENGLISH ABSTRACT: The cultivation of table grapes in the warmer areas of South Africa, indeed worldwide, is complicated by rest breaking problems in spring due to delayed budbreak. In order to overcome these problems rest breaking agents, mainly hydrogen cyanamide, are applied. However, instead of alleviating the problem, additional problems such as uneven budbreak and reduced production are often induced. This study was initiated to further understand the physiological processes occurring during budbreak and how the application of hydrogen cyanamide influences these processes. The following aspects were investigated in this study: a. The effect of hydrogen cyanamide on tissue cytokinin (specifically zeatin riboside) levels of Sultanina table grape vines after application at different times before natural budbreak was studied over two seasons. In 1997, hydrogen cyanamide was applied at three weeks before induced budbreak and in 1998 at six weeks before induced budbreak. One year-old canes were sampled weekly after hydrogen cyanamide application, divided into distal and proximal sections, then further divided into buds, bark and wood tissues and the zeatin riboside (ZR) levels determined. A relatively high amount of chilling coupled to late hydrogen cyanamide application in 1997 led to a large effect on ZR release, but did not lead to significant shifting of the budbreak pattern. Zeatin riboside peaks were observed in buds, internode wood and bark of treated vines compared to control vines. The peaks were higher in distal portions compared to proximal portions in all tissues. The relatively lower chilling and earlier application of hydrogen cyanamide in 1998 had a larger effect on the budbreak pattern while the bud ZR peak was shifted earlier. The distal portion bud ZR . peak was again higher than the proximal portion bud ZR peak. In 1997, as sampling was not initiated early enough, bud ZR peaks were only observed after budbreak, while in 1998 bud ZR peaks were observed before and after budbreak. The effect of these ZR increases on the development of inflorescence primordia, subsequent bunch development and ultimately production, are discussed. b. Free xylem sap was sampled at cane and spur pruned lengths from unpruned canes of Sultanina from budswell until after budbreak in 1999 and from three table grape cultivars, i.e Sultanina, Alphonse Lavalleé and Sunred Seedless, in 2001 and ZR levels determined. The ZR levels in the buds of these three table grape cultivars, pruned to different cane lengths were also determined. One year old canes of these cultivars, were each pruned to long canes (14 buds) and short spurs (2 buds). The ZR content in buds of these canes at distal and proximal positions were determined weekly from budswell until after budbreak in 1999. Xylary ZR peaks occurred before 50% budbreak. Spur xylary ZR levels of all three cultivars followed a similar pattern, although at lower ZR levels than that of the canes. This is similar to previous studies on xylary ZR levels of apple shoots. The high levels of free ZR found in xylem sap at the distal portions of canes support the hypothesis of a cumulative ZR build-up effect as cane length increases. Spur pruning resulted in earlier budbreak and a higher final budbreak than cane pruning. The proximal portions of shoots, whether spur pruned or the proximal portions of canes, showed elevated ZR levels in all cultivars. This difference in ZR levels in bud tissue of different portions of the cane would suggest a difference in ZR consumption or turnover. The results of this study have important management implications for the cultivation of vines in warmer areas in which hydrogen cyanamide is used to alleviate budbreak problems. / AFRIKAANSE OPSOMMING: Die verbouing van tafeldruiwe in die warmer gebiede van Suid-Afrika, soos straks wêreldwyd, word bemoeilik deur rusbreekprobleme in die lente weens vertraagde bot. Rusbreekmiddels, meestal waterstofsiaanamied, word gevolglik toegedien om hierdie probleme te probeer oorkom. In plaas van opheffing van die probleem, veroorsaak hierdie toedienings somtyds addisionele probleme soos ongelyke bot en verlaagde produksie. Hierdie studie is aangepak om die fisiologiese prosesse wat tydens bot plaasvind, beter te verstaan, asook hoe die toediening van waterstofsiaanamied hierdie prosesse beïnvloed. Die volgende is in hierdie studie ondersoek: a. Die invloed van waterstofsiaanamied op lootweefsel sitokinien (naamlik zeatin ribosied, ZR) vlakke van 'n Sultanina tafeldruif wingerd is oor twee seisoene ondersoek na toediening op verskillende tye voor bot. Waterstofsiaanamied is in 1997 drie weke en in 1998 ses weke voor geïnduseerde bot toegedien. Een jaar oue lote is weekliks gemonster na waterstofsiaanamied toediening, verdeel in apikale en basale gedeeltes en verder verdeel in ogies, bas en houtweefsel en die ZR vlakke bepaal. Relatief hoër vlakke koue, gekoppel met later watersiaanamied toediening het in 1997 tot 'n groot effek op ZR vlakke gelei, maar het nie die botpatroon wesentlik verskuif nie. Zeatin ribosied pieke is waargeneem in ogies, internode hout en bas van behandelde wingerd in vergelyking met kontrole wingerd. Die pieke was hoër in apikale gedeeltes in vergelyking met basale gedeeltes in alle weefsels. Die relatief laer koue en vroeër toediening van waterstofsiaanamied in 1998 het 'n groter effek op die botpatroon gehad, terwyl die ogie ZR piek vroeër geskuif is. Die apikale gedeelte se ogie ZR piek was weereens hoër as die basale gedeelte se ogie ZR piek. Monstering was nie vroeg genoeg begin in 1997 nie, aangesien ogie ZR pieke slegs na bot waargeneem is, terwylogie ZR pieke in 1998 voor en na bot waargeneem is. Die effek van hierdie verhoging in ZR vlakke op die ontwikkeling van blom primordia, daaropvolgende tros ontwikkeling en uiteindelik produksie, is bespreek. b. Vry xileemsap is gemonster van langdraer en kortdraer gedeeltes van ongesnoeide lote van Sultanina vanaf ogieswel tot na bot in 1999 en vanaf drie tafeldruif kultivars, naamlik Sultanina, Alphonse lavalleé en Sunred Seedless, in 2001 en die ZR vlakke bepaal. Die ZR vlakke van ogies van hierdie drie kultivars, gesnoei tot verskillende lootlengtes is ook bepaal. Eenjarige lote van hierdie kultivars is gesnoei tot langdraers (14 ogies) en kortdraers (2 ogies). Die ZR vlakke in ogies geleë op apikale en basale gedeeltes van hierdie lote is weekliks in 1999 bepaal vanaf ogieswel tot na bot Xileemsap ZR pieke is waargeneem voor 50% bot. Kortdraer xileemsap ZR vlakke het 'n soortgelyke patroon as die langdraers gevolg vir al drie kultivars, alhoewel teen laer ZR vlakke as die langdraers. Hierdie waarneming is soortgelyk aan vorige studies op xileemsap ZR vlakke van appel lote. Die hoë ZR vlakke gevind in die xileemsap van die apikale gedeeltes van lote ondersteun die hipotese van 'n kumulatiewe opbou van ZR soos die lengte van die loot toeneem. Die snoei van lote as kortdraers het gelei tot vroeër bot en 'n hoër finale bot persentasie as die snoei van langdraers. Die basale gedeeltes van lote het verhoogde ZR vlakke in al drie kultivars getoon, onafhanklik van die feit dat dit vanaf die kortdraer of die basale gedeelte van 'n langdraer was. Hierdie verskil in ZR vlakke in ogie weefsel van verskillende gedeeltes van die loot impliseer 'n verskil in ZR verbruik of omset. The resultate van hierdie studie het belangrike bestuursimplikasies vir die verbouing van wingerd in warmer gebiede, waar waterstofsiaanamied gebruik word om botprobleme te oorkom.

A área de Cerrado escolhida para o estudo está em regeneração na Estação Ecológica de Santa Bárbara, localizada no município de Águas de Santa Bárbara, SP. Nesta área o Pinus, que estava sob cultivo desde os anos 70, foi retirado em 2012 e, em 2014, foi realizada uma queimada. As espécies Eugenia dysenterica (Mart.) DC. e E. punicifolia (Kunth) DC. foram escolhidas devido ao elevado número de indivíduos que rebrotaram na área. O objetivo geral do projeto foi conduzir análises morfoanatômicas e químicas das estruturas relacionadas ao potencial de rebrotamento. Foram realizadas: a contagem das gemas subterrâneas nos primeiros 10 centímetros abaixo do nível do solo, as análises químicas das raízes e análises anatômicas na região terminal de ramos aéreos e no sistema subterrâneo. Nas duas espécies, além da gema apical dos ramos aéreos, nas três regiões nodais subsequentes, acima da gema axilar ocorre uma gema acessória. Em todas as gemas, observam-se estruturas de proteção, pois junto ao meristema apical caulinar (MAC) ocorrem coléteres e cristais. Além disso, o MAC é protegido por primórdios foliares com cavidades de óleo, muitos cristais e tricomas unicelulares não glandulares que possuem espessamento parietal em celulose e podem acumular compostos fenólicos. Nos sistemas subterrâneos, o número de gemas, localizadas preferencialmente nos primeiros cinco centímetros do solo, variou entre os três indivíduos da mesma espécie: E. dysenterica 162, 17 e 253, E. punicifolia 24, 40 e 109. Esta variação deveu-se ao grau de desenvolvimento das estruturas subterrâneas as quais certamente formaram-se antes do período da retirada do Pinus. O sistema subterrâneo das espécies é formado por um eixo lenhoso cuja porção superior é caulinar e emite vários ramos aéreos enquanto a porção inferior é constituída por uma raiz axial espessada que pode atingir até um metro de profundidade em E. dysenterica. Em E. punicifolia o sistema subterrâneo ocupa uma região mais superficial do solo e, assim como o caule, as raízes axial e adventícias são distribuídas num plano mais horizontal. O tecido de revestimento nas raízes de E. punicifolia e no caule e raízes de E. dysenterica, apresenta camadas alternadas de células de paredes suberizadas e de células com espessamentos parietais em pectina; no caule de E. punicifolia há esclereides que se alternam com as células suberizadas. Em E. punicifolia o espessamento resulta de divisões anticlinais e expansão tangencial das células do parênquima floemático. Há floema interno e parênquima medular nos caules subterrâneos de ambas as espécies. Compostos fenólicos e grãos de amido estão presentes nas células parenquimáticas de regiões mais espessadas dos caules e raízes. Os teores de carboidratos totais, hemicelulose, celulose e lignina não diferem significativamente nas duas espécies. No entanto, as concentrações de flavonoides totais e de compostos fenólicos totais foram superiores nas raízes de E. punicifolia. O grau de proteção das gemas aéreas, o elevado número de gemas e o acúmulo de compostos de reserva (amido) e de proteção (fenóis, flavonoides, lignina) nas estruturas subterrâneas podem ter favorecido o seu rebrotamento após a retirada do Pinus e a sua permanência das espécies na área. / The Cerrado area studied is under regeneration at the Estação Ecológica de Santa Bárbara, located in Águas de Santa Bárbara (São Paulo, Brazil). In this area, the Pinus, cultivated since the 1970s, was removed in 2012 and then, in 2014, the area was burnt. The species Eugenia dysenterica (Mart.) DC. and E. punicifolia (Kunth) DC. were studied due to their high resprouting after the burnt event. The aim was to carry out morphoanatomical and chemical analyses of the structures related to the resprouting capacity. The number of belowground buds was counted in the first 10 cm below the soil surface. The chemical analyses of the roots and anatomical analyses of the end portion of the aerial branches and the underground system were performed. Besides the apical bud, both species showed an accessory bud above the axillary one at the three subsequent nodal regions. In all studied buds, protection structures were observed, as colleters and crystals occur close to the shoot apical meristem (SAM). Moreover, SAM is protected by leaf primordia with oil cavities, many crystals and non-glandular unicellular trichomes. These trichomes present parietal cellulose thickening and are able to accumulate phenolic compounds. In underground systems, the number of buds, mostly in the first five centimeters below the soil surface, varied between individuals per species: E. dysenterica 162, 17 and 253; E. punicifolia 24, 40 and 109. This variation was caused by the development degree of the underground system, whose structures were certainly developed before Pinus removal. The underground system of the species display a woody axis, whose upper portion is a stem structure and produces several aerial branches. Its lower part is formed by an axial thickened root that can reach one metre deep in E. dysenterica. In E. punicifolia, the subterranean system occupies a more superficial region of the soil and, similar to the stem, the axial and adventitious roots are distributed in a more horizontal plane. The covering tissue of underground systems in E. punicifolia and E. dysenterica displays alternate layers of cells with suberized walls and pectin-thickened walls. In E. punicifolia stem, the covering tissue exhibits sclereids alternate with cells of suberized walls. The thickening in E. punicifolia is a result of anticlinal divisions and tangential expansion of phloematic parenchyma cells. Both species show internal phloem and medullary parenchyma in underground stems. Phenolic compounds and starch grains are present in parenchyma cells of thickened regions in stems and roots. The contents of total carbohydrates, hemicellulose, cellulose and lignin do not differ significantly in the two species. However, concentrations of total flavonoids and total phenolic compounds are higher in roots of E. punicifolia. The protection level of aerial buds, large number of underground buds, and accumulation of storage (starch) and protection (phenols, flavonoids, lignin) compounds in the underground structures may have favoured resprouting of the species after Pinus removal and their maintenance in the area.

As alterações do paladar durante o transplante de células tronco hematopoiéticas (TCTH) são frequentemente descritas em períodos após a finalização do transplante, porém pouco se conhece sobre quais sabores e que tipo de alterações de percepção são vivenciadas pelos pacientes durante o transplante. O objetivo deste trabalho foi caracterizar as alterações do paladar e de despapilação presentes no período de neutropenia do TCTH, bem como verificar se há associação entre essas alterações e variáveis clínicas relacionadas ao transplante e à toxicidade na mucosa oral. Também se avaliou o impacto das alterações do paladar e da mucosa oral na qualidade de vida dos pacientes. Foram selecionados 52 pacientes de TCTH autólogo e alogênico, que realizaram testes de acuidade do paladar antes do condicionamento e durante a neutropenia. Os pacientes foram ainda submetidos a oroscopia para avaliação de mucosite oral e despapilação no dorso da língua. Foi também realizado inquérito quanto a xerostomia e a alterações no paladar. Foi realizado teste de acuidade na percepção dos sabores doce, amargo, azedo e salgado, ofertados em soluções contendo concentrações baixas e altas de substâncias estimuladoras desses sabores. A qualidade de vida foi avaliada pelo questionário EORTC QLQ-C30 e QLQ-H&N35. Detectou-se que os 46,1% dos pacientes exibiram alterações do paladar antes do condicionamento do TCTH, mas que essa frequência aumentou para 90,5% durante a neutropenia (teste do c2, p=0.042). O tipo de alteração mais comum foi a hipogeusia dos sabores de concentração mais forte, principalmente do amargo. Alta frequência de pacientes (72,0%) foi detectada exibindo despapilação no período de neutropenia, porém essa despapilação não foi associada às alterações de percepção de cada tipo de sabor. Não houve associação entre alterações do paladar e tipo de transplante, tipo de condicionamento e variáveis relacionadas à toxicidade na cavidade oral e no trato gastrointestinal. Houve associação significativa entre despapilação e duração da mucosite oral >=8 dias (OR= 5,62, IC95% = 0,98-60,30, p=0.039). As alterações salivares e do paladar reduziram significativamente na qualidade de vida durante a neutropenia em comparação ao período que antecedeu o condicionamento. Concluiu-se que as alterações do paladar já estão presentes antes do TCTH, mas há aumento da frequência dessas alterações, principalmente de hipogeusia. A despapilação na língua ocorreu após o condicionamento, e foi associada a maior tempo de duração da mucosite oral. O impacto das alterações salivares e do paladar na qualidade de vida do paciente durante a neutropenia é alto e devem ser minimizados mediante a adoção de estratégias mais abrangentes, que incluam manutenção da integridade da mucosa oral. / Taste changes during hematopoietic cell transplantation (HCT) are often described after the end of transplantation, however little is known about which flavors and what type of perception changes are experienced by patients during transplantation. The aim of this study was to characterize the taste alterations and taste buds atrophy present in the period of neutropenia of HCT, as well as to verify if there is association between these alterations and clinical variables related to transplant and oral mucosal toxicity. The impact of taste and oral mucosal changes on patients\' quality of life was also evaluated. We selected 52 patients undergoing autologous and allogenic HCT, who underwent taste acuity tests prior to conditioning and during neutropenia. Patients were also submitted to oroscopy for evaluation of oral mucositis and taste buds atrophy. An investigation was also conducted on xerostomia and taste changes. It was carried out an acuity test in the perception of sweet, bitter, sour and salty flavors, offered in solutions containing low and high concentrations of substances stimulating these flavors. Quality of life was assessed by the EORTC questionnaire QLQ-C30 and QLQ-H & N35. It was found that 46.1% of the patients exhibited taste alterations before the conditioning of the HCT, but that this frequency increased to 90.5% during neutropenia (c2 test, p = 0.042). The most common alteration was the hypogeusia of the strongest concentrations, especially bitter. High frequency of patients (72.0%) were detected with taste buds atrophy in the period of neutropenia, but this taste buds atrophy was not associated with alterations in the perception of each type of taste. There was no association between changes in taste and type of transplant, type of conditioning, and variables related to toxicity in the oral cavity and in the gastrointestinal tract. There was a significant association between taste buds atrophy and oral mucositis duration >=8 days (OR = 5.62, 95% CI = 0.98-60.30, p = 0.039). Salivary and taste changes significantly reduced quality of life during neutropenia compared to the pre-condition period. It was concluded that taste alterations are already present before HCT, but there is an increase in the frequency of these alterations, mainly hypogeusia. The tongue taste buds atrophy occurred after conditioning, and was associated with a longer duration of oral mucositis. The impact of salivary and taste changes on patient quality of life during neutropenia is high and should be minimized through the adoption of more comprehensive strategies that include maintenance of oral mucosal integrity.

A área de Cerrado escolhida para o estudo está em regeneração na Estação Ecológica de Santa Bárbara, localizada no município de Águas de Santa Bárbara, SP. Nesta área o Pinus, que estava sob cultivo desde os anos 70, foi retirado em 2012 e, em 2014, foi realizada uma queimada. As espécies Eugenia dysenterica (Mart.) DC. e E. punicifolia (Kunth) DC. foram escolhidas devido ao elevado número de indivíduos que rebrotaram na área. O objetivo geral do projeto foi conduzir análises morfoanatômicas e químicas das estruturas relacionadas ao potencial de rebrotamento. Foram realizadas: a contagem das gemas subterrâneas nos primeiros 10 centímetros abaixo do nível do solo, as análises químicas das raízes e análises anatômicas na região terminal de ramos aéreos e no sistema subterrâneo. Nas duas espécies, além da gema apical dos ramos aéreos, nas três regiões nodais subsequentes, acima da gema axilar ocorre uma gema acessória. Em todas as gemas, observam-se estruturas de proteção, pois junto ao meristema apical caulinar (MAC) ocorrem coléteres e cristais. Além disso, o MAC é protegido por primórdios foliares com cavidades de óleo, muitos cristais e tricomas unicelulares não glandulares que possuem espessamento parietal em celulose e podem acumular compostos fenólicos. Nos sistemas subterrâneos, o número de gemas, localizadas preferencialmente nos primeiros cinco centímetros do solo, variou entre os três indivíduos da mesma espécie: E. dysenterica 162, 17 e 253, E. punicifolia 24, 40 e 109. Esta variação deveu-se ao grau de desenvolvimento das estruturas subterrâneas as quais certamente formaram-se antes do período da retirada do Pinus. O sistema subterrâneo das espécies é formado por um eixo lenhoso cuja porção superior é caulinar e emite vários ramos aéreos enquanto a porção inferior é constituída por uma raiz axial espessada que pode atingir até um metro de profundidade em E. dysenterica. Em E. punicifolia o sistema subterrâneo ocupa uma região mais superficial do solo e, assim como o caule, as raízes axial e adventícias são distribuídas num plano mais horizontal. O tecido de revestimento nas raízes de E. punicifolia e no caule e raízes de E. dysenterica, apresenta camadas alternadas de células de paredes suberizadas e de células com espessamentos parietais em pectina; no caule de E. punicifolia há esclereides que se alternam com as células suberizadas. Em E. punicifolia o espessamento resulta de divisões anticlinais e expansão tangencial das células do parênquima floemático. Há floema interno e parênquima medular nos caules subterrâneos de ambas as espécies. Compostos fenólicos e grãos de amido estão presentes nas células parenquimáticas de regiões mais espessadas dos caules e raízes. Os teores de carboidratos totais, hemicelulose, celulose e lignina não diferem significativamente nas duas espécies. No entanto, as concentrações de flavonoides totais e de compostos fenólicos totais foram superiores nas raízes de E. punicifolia. O grau de proteção das gemas aéreas, o elevado número de gemas e o acúmulo de compostos de reserva (amido) e de proteção (fenóis, flavonoides, lignina) nas estruturas subterrâneas podem ter favorecido o seu rebrotamento após a retirada do Pinus e a sua permanência das espécies na área. / The Cerrado area studied is under regeneration at the Estação Ecológica de Santa Bárbara, located in Águas de Santa Bárbara (São Paulo, Brazil). In this area, the Pinus, cultivated since the 1970s, was removed in 2012 and then, in 2014, the area was burnt. The species Eugenia dysenterica (Mart.) DC. and E. punicifolia (Kunth) DC. were studied due to their high resprouting after the burnt event. The aim was to carry out morphoanatomical and chemical analyses of the structures related to the resprouting capacity. The number of belowground buds was counted in the first 10 cm below the soil surface. The chemical analyses of the roots and anatomical analyses of the end portion of the aerial branches and the underground system were performed. Besides the apical bud, both species showed an accessory bud above the axillary one at the three subsequent nodal regions. In all studied buds, protection structures were observed, as colleters and crystals occur close to the shoot apical meristem (SAM). Moreover, SAM is protected by leaf primordia with oil cavities, many crystals and non-glandular unicellular trichomes. These trichomes present parietal cellulose thickening and are able to accumulate phenolic compounds. In underground systems, the number of buds, mostly in the first five centimeters below the soil surface, varied between individuals per species: E. dysenterica 162, 17 and 253; E. punicifolia 24, 40 and 109. This variation was caused by the development degree of the underground system, whose structures were certainly developed before Pinus removal. The underground system of the species display a woody axis, whose upper portion is a stem structure and produces several aerial branches. Its lower part is formed by an axial thickened root that can reach one metre deep in E. dysenterica. In E. punicifolia, the subterranean system occupies a more superficial region of the soil and, similar to the stem, the axial and adventitious roots are distributed in a more horizontal plane. The covering tissue of underground systems in E. punicifolia and E. dysenterica displays alternate layers of cells with suberized walls and pectin-thickened walls. In E. punicifolia stem, the covering tissue exhibits sclereids alternate with cells of suberized walls. The thickening in E. punicifolia is a result of anticlinal divisions and tangential expansion of phloematic parenchyma cells. Both species show internal phloem and medullary parenchyma in underground stems. Phenolic compounds and starch grains are present in parenchyma cells of thickened regions in stems and roots. The contents of total carbohydrates, hemicellulose, cellulose and lignin do not differ significantly in the two species. However, concentrations of total flavonoids and total phenolic compounds are higher in roots of E. punicifolia. The protection level of aerial buds, large number of underground buds, and accumulation of storage (starch) and protection (phenols, flavonoids, lignin) compounds in the underground structures may have favoured resprouting of the species after Pinus removal and their maintenance in the area.