Diss. (sammanfattning) Umeå : Univ., 2007. / Härtill 4 uppsatser.

La simulation numérique fiable des performances de turbines hydrauliques suppose : i) de pouvoir inclure dans les calculs RANS (Reynolds-Averaged Navier-Stokes) traditionnellement mis en œuvre l'effet des incertitudes qui existent en pratique sur les conditions d'entrée de l'écoulement; ii) de pouvoir faire appel à une stratégie de type SGE (Simulation des Grandes Echelles) pour améliorer la description des effets de la turbulence lorsque des écarts subsistent entre calculs RANS et résultats d'essai de référence même après prise en compte des incertitudes. Les présents travaux mettent en oeuvre une démarche non intrusive de quantification d'incertitude (NISP pour Non-Intrusive Spectral Projection) pour deux configurations d'intérêt pratique : un distributeur de turbine Francis avec débit et angle d'entrée incertains et un aspirateur de turbine bulbe avec conditions d'entrée (profils de vitesse,en particulier en proche paroi, et grandeurs turbulentes) incertaines. L'approche NISP est utilisée non seulement pour estimer la valeur moyenne et la variance de quantités d'intérêt mais également pour disposer d'une analyse de la variance qui permet d'identifier les incertitudes les plus influentes. Les simulations RANS, vérifiées par une démarche de convergence en maillage, ne permettent pas pour la plupart des configurations analysées d'expliquer les écarts calcul / expérience grâce à la prise en compte des incertitudes d'entrée.Nous mettons donc également en ouvre des simulations SGE en faisant appel à une stratégie originale d'évaluation de la qualité des maillages utilisés dans le cadre d'une démarche de vérification des calculs SGE. Pour une majorité des configurations analysées, la combinaison d'une stratégie SGE et d'une démarche de quantification des incertitudes permet de produire des résultats numériques fiables. La prise en compte des incertitudes d'entrée permet également de proposer une démarche d'optimisation robuste du distributeur de turbine Francis étudié. / The reliable numerical simulation of hydraulic turbines performance requires : i) to includeinto the conventional RANS computations the effect of the uncertainties existing in practiceon the inflow conditions; ii) to rely on a LES (Large Eddy Simulation) strategy to improve thedescription of turbulence effects when discrepancies between RANS computations and experimentskeep arising even though uncertainties are taken into account. The present workapplies a non-intrusive Uncertainty Quantification strategy (NISP for Non-Intrusive SpectralProjection) to two configurations of practical interest : a Francis turbine distributor, with uncertaininlet flow rate and angle, and a draft-tube of a bulb-type turbine with uncertain inflowconditions (velocity distributions, in particular close to the wall boundaries, and turbulentquantities). The NISP method is not only used to compute the mean value and variance ofquantities of interest, it is also applied to perform an analysis of the variance and identify inthis way the most influential uncertainties. The RANS simulations, verified through a gridconvergence approach, are such the discrepancies between computation and experimentcannot be explained by taking into account the inflow uncertainties for most of the configurationsunder study. Therefore, LES simulations are also performed and these simulations areverified using an original methodology for assessing the quality of the computational grids(since the grid-convergence concept is not relevant for LES). For most of the flows understudy, combining a SGE strategy with a UQ approach yields reliable numerical results. Takinginto account inflow uncertainties also allows to propose a robust optimization strategy forthe Francis turbine distributor under study.

Submitted by Camila Paula Rossetto Pescatori Jacon (capescatori@fca.unesp.br) on 2016-11-22T10:47:40Z No. of bitstreams: 1 Tese completa impressa.pdf: 5118013 bytes, checksum: 4331829f5c23efb70accd8824afcccfc (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-11-25T16:33:07Z (GMT) No. of bitstreams: 1 jacon_cprp_dr_bot.pdf: 5118013 bytes, checksum: 4331829f5c23efb70accd8824afcccfc (MD5) / Made available in DSpace on 2016-11-25T16:33:07Z (GMT). No. of bitstreams: 1 jacon_cprp_dr_bot.pdf: 5118013 bytes, checksum: 4331829f5c23efb70accd8824afcccfc (MD5) Previous issue date: 2016-08-05 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A utilização de plantas livres de vírus, associada a outras tecnologias, pode proporcionar maior produtividade no cultivo de alho vernalizado. No entanto, essas plantas apresentam comportamento produtivo distinto das plantas infectadas, tornando necessária a adequação das técnicas de manejo empregadas na cultura até então. Estudos sobre a nutrição das plantas de alho livres de vírus ainda não são conclusivos. Visando alcançar alta produtividade, e considerando as características dos solos tropicais, os adubos fosfatados têm sido utilizados em altas doses. Então, o presente trabalho teve o objetivo de verificar o efeito da adubação fosfatada no cultivo de alho vernalizado livre de vírus. Para tanto foram conduzidos três experimentos em anos subsequentes, em área de produção, na região de Santa Juliana – MG. No primeiro experimento foram avaliadas duas fontes (100% superfosfato simples: SS e 50% superfosfato simples + 50% termofosfato: SS+T) e 5 doses de fósforo (0, 100, 200, 300, 400 mg dm-3) em 4 repetições. Observou-se que houve pouca influência das fontes de fósforo utilizadas nos resultados e que as maiores doses de P utilizadas ocasionaram redução na produção e qualidade dos bulbos. No experimento 2 foram avaliadas 5 doses de P (0, 70, 140, 210, 180 e 350 mg dm- 3 ) em 4 repetições, utilizando-se a fonte SS+T. Verificou-se que, da mesma forma que no primeiro experimento, nas doses mais elevadas de P ocorreu redução da produção. Devido a uma chuva ocorrida na fase de diferenciação, ocorreu o superbrotamento, verificando-se aumento da incidência de plantas com essa anomalia com a elevação das doses de P. O terceiro experimento foi conduzido avaliando-se 5 doses de P (0, 50, 100, 150, 200 e 250 mg dm-3) em 4 repetições e também foi verificada redução na produção na dose mais elevada de P. A dose de 200 mg dm-3 de P, utilizando-se a fonte superfosfato simples + termofosfato, em solo com alto teor inicial de fósforo, proporcionou maior produção e porcentagem de bulbos nas classes mais valorizadas. / The use of virus-free garlic plants, associated with other technologies can increased productivity in vernalized garlic growing. However, these plants have different productive behavior from the infected plants, requiring the adequacy of management techniques used for the culture until then. Studies on virus-free garlic plants nutrition of are not conclusive. In order to achieve high productivity and considering the characteristics of tropical soil, phosphate fertilizers have been used in high doses. So, this study aimed to vertify the effect of phosphate fertilization on vernalized virus-free garlic growing. Therefore three experiments were carried out in subsequent years in growing field, in the Santa Juliana - MG. In the first experiment were evaluated two sources (SS and SS + T) and 5 doses of phosphorus (0, 100, 200, 300, 400 mg dm-3) in 4 replications. It was observed that the results were little influenced by the P sources and that higher doses of P applied occasioned reduction in yield and quality of the bulbs. In experiment 2 were evaluated five doses of P (0, 70, 140, 210, 180 and 350 mg dm-3) in 4 replicates using SS+T. It was found that, as in the first experiment, at the higher doses of P, there was a decrease of production. Due to a rain occurred in the differentiation stage, there was incidence of secondary bulb growth and it was verified that plants with this anomaly increased with P doses rising. The third experiment was carried out evaluating five levels of P (0, 50, 100, 150, 200 and 250 mg dm-3) in 4 replicates and it was also verified that there was a decrease in production at the highest dose. The use of 200 mg dm-3 of P, with the source superphosphate + thermophosphate, in soil with high phosphorus initial level, provided higher yield and percentage of bulbs in more valued classes.

O presente trabalho descreve um programa de computador em linguagem Java que reproduz o modelo compartimental reduzido de célula mitral do bulbo olfativo construído por Davison, Feng e Brown (Brain Res. Bull. 51:393-399,2000), como uma simplificação do modelo detalhado de Bhalla e Bower (J. Neurophysiol., 69:1948-1965, 1993). O modelo reduzido considera a célula mitral como composta por quatro compartimentos, modelados conforme a metodologia de HODGKIN e HUXLEY. Por seu baixo custo computacional, o modelo reduzido permite a construção de modelos de rede de grande porte para o bulbo olfativo. A implementação computacional feita em Java apresenta grande similaridade com a original, indicando uma robustez do modelo com relação a versões em plataformas distintas. / This work describes a computer program written in Java, which reproduces the reduced compartimental model of the mitral cell of the olfactory bulb constructed by Davison, Feng and Brown (Brain Res. Bull. 51:393-399,2000), as a simplified version of the detailed model of Bhalla and Bower (J. Neurophysiol., 69:1948-1965, 1993). The reduced model considers the mitral cell as composed of four compartiments modeled according to the Hodgkin-Huxley formalism. Due to its low computational cost, the reduced model allows the construction of large-scale network models of the olfactory bulb. The computer implementation made in Java shows great similarity with the original, indicating that the model is robust with respect to implementations in different platforms.

O sistema da procineticina desempenha um papel importante na migração dos neurônios secretores de GnRH e na neurogênese do bulbo olfatório. Camundongos com ablação dos genes que codificam a procineticina 2 (PROK2) e seu receptor (PROKR2) apresentaram fenótipos semelhantes ao da síndrome de Kallmann descrita em humanos. Mutações inativadoras nos genes PROK2 e PROKR2 foram identificadas em pacientes com hipogonadismo hipogonadotrófico isolado. Com base nestes achados, investigamos a presença de alterações estruturais nos genes PROK2 e PROKR2 em 107 pacientes brasileiros (63 com síndrome de Kallmann e 47 com hipogonadismo hipogonadotrófico isolado normósmico). Cem indivíduos brasileiros que relataram desenvolvimento puberal normal foram utilizados como grupo controle. As regiões codificadoras dos genes PROK2 e PROKR2 foram amplificadas utilizando-se oligonucleotídeos intrônicos específicos, seguida de purificação enzimática e sequenciamento automático. Duas mutações no gene PROK2 foram identificadas: a mutação p.G100fsX121 em homozigose presente em dois irmãos com síndrome de Kallmann; e a mutação p.I55fsX56 em heterozigose identiificada em um paciente com HHIn. Quatro mutações foram identificadas no gene PROKR2 (p.R80C, p.Y140X, p.L173R e p.R268C) em cinco pacientes com síndrome de Kallmann e um paciente com HHIn. Essas mutações não foram encontradas no grupo controle. As mutações do tipo missense, p.R80C, p.L173R e p.R268C foram identificadas em heterozigose. Mutações nos genes FGFR1, GnRHR, KiSS-1 e GPR54 foram excluídas nesses pacientes. O paciente portador da mutação p.R268C do PROKR2 apresentou deleção dos exons 1 e 2 do gene KAL1. Adicionalmente, as mutações p.R80C e p.R268C foram identificadas em heterozigose em parentes de primeiro grau assintomáticos dos casos índices. A nova mutação p.Y140X do PROKR2, única alteração em homozigose, foi identificada em um paciente com micropênis, criptorquidia bilateral, anosmia e palato ogival. Os pais deste paciente eram portadores da mutação p.Y140X em heterozigose e relataram desenvolvimento puberal normal e ausência de anormalidades olfatórias. Estudos in vitro da nova mutação p.R80C localizada na primeira alça intracelular demonstraram que o acúmulo de fofatidil-inositol (IP), assim como a ativação da via da MAPK foram significativamente afetadas em células transfectadas com o receptor mutado em relação ao receptor selvagem, indicando que a mutação p.R80C determina uma menor atividade do receptor. Avaliação da expressão por Western blot mostrou uma diminuição na expressão do receptor mutado R80C e uma maior expressão de receptores imaturos. Esses achados sugeriram o papel crítico da arginina localizada na posição 80 na atividade normal do receptor. Em conclusão, expandimos o repertório de mutações deletérias nos genes PROK2 e PROKR2 em pacientes com hipogonadismo hipogonadotrófico isolado. A haploinsuficiência do PROKR2 não foi suficiente para causar síndrome de Kallmann ou HHIn, entretanto mutações inativadoras em homozigose nos genes PROK2 e PROKR2 foram responsáveis pelo fenótipo reprodutivo e olfatório anormal, em concordância com os estudos prévios de ablação gênica em modelos animais. Arginina localizada na posição 80 do PROKR2 desempenha um papel crucial na adequada maturação do receptor / Physiological activation of the prokineticin pathway has a critical role in olfactory bulb morphogenesis and GnRH secretion. Knock-out mice for genes that encode prokineticin 2 (PROK2) and the prokineticin receptor 2 (PROKR2) exhibited a phenotype similar to the Kallmann syndrome (KS). Inactivating mutations in PROK2 and PROKR2 have been identified in patients with isolated hypogonadotropic hypogonadism. Based on these findings, we investigated the presence of inactivating mutations of the genes PROK2 and PROKR2 in Brazilian patients with isolated hypogonadotropic hypogonadism associated or not with olfactory abnormalities and performed in vitro studies of the new identified mutations. We studied 107 patients with HH (63 with Kallmann syndrome and 44 with normosmic HH) and 100 control individuals. The coding regions of PROK2 and PROKR2 were amplified by polymerase chain reaction followed by enzymatic purification and direct automatic sequencing. In PROK2, two known frameshift mutations were identified. Two brothers with Kallmann syndrome harbored the homozygous p.G100fsX121 mutation, whereas one male with normosmic HH harbored the heterozygous p.I55fsX56 mutation. In PROKR2, four distinct mutations (p.R80C, p.Y140X, p.L173R and p.R268C) were identified in five patients with Kallmann syndrome and in one patient with normosmic HH. These mutations were not found in the control group. The p.R80C and p.R268C missense mutations were identified in heterozygous state in the HH patients and in their asymptomatic first-degree relatives. The p.L173R was also identified in heterozygous state. In addition, no mutations of FGFR1, GnRHR, KiSS-1 or GPR54 were identified in these patients. The patient with the PROKR2 mutation p.R268C also has a deletion of the exon 1 and 2 in the gene KAL1. Notably, the new nonsense mutation (p.Y140X) was identified in homozygous state in an anosmic boy with micropenis, bilateral cryptorchidism and high-arched palate. His asymptomatic parents were heterozygous for this severe defect. In vitro studies of the new mutation, p.R80C, were performed in order to access the mechanism by which this mutation could affect the activity of the PROKR2. In vitro studies showed that the amount of fofatidil-inositol (PI) and the activation of MAPK were significantly lower in cells transfected with the R80C mutant receptor than in cells transfected with the wild receptor, indicating that this variant is a loss-of-function mutation. Binding studies and Western blot showed a reduction in the expression levels of the receptor in the plasma membrane and in whole cell, respectively. Additionally, Western blot analysis of R80C PROKR2 revealed an additional smaller molecular weight band that represents the presence of immature unglycosylated receptors. The arginine 80 in ICL1 is important for post-translational processing of PROKR2. In conclusion, we expanded the repertoire of PROK2 and PROKR2 mutations in patients with HH and showed that PROKR2 haploinsufficiency is not sufficient to cause Kallmann syndrome or normosmic HH, whereas homozygous loss-of-function mutations either in PROK2 or PROKR2 are sufficient to cause disease phenotype, in accordance with the Prokr2 and Prok2 knockout mouse models. In vitro studies suggested that the arginine located at position 80 of the receptor seems to play an important role in the receptor function

L’aspirateur d’une centrale hydroélectrique est l’organe hydraulique se situant en aval de la roue. Il a une forme divergente afin de récupérer l’énergie cinétique résiduelle en sortie de roue sous forme de pression statique et augmenter ainsi la chute nette de la centrale. Dans le cas des turbines de basse chute de type bulbe, les pertes de charge dans l’aspirateur influencent fortement le rendement global de la centrale. La prédiction correcte de ces pertes de charge au cours du dimensionnement de la turbine représente donc un enjeu majeur. La prédiction numérique des pertes de charge dans l’aspirateur est un réel challenge car l’écoulement dans l’aspirateur est dynamiquement complexe avec des nombres de Reynolds élevés, la présence de swirl et d’un gradient adverse de pression. Ces caractéristiques font que les approches de modélisation classiquement utilisées dans l’industrie sont mises en défaut. L’objectif de ce travail est double : (i) améliorer la prédiction de l’écoulement turbulent dans l’aspirateur en utilisant des approches instationnaires URANS et LES et en portant une attention particulière à la description des conditions d’entrée de l’aspirateur et (ii) réaliser une analyse fine des échanges énergétiques dans l’aspirateur pour mieux comprendre l’origine des pertes de charge. Une condition d’entrée instationnaire représentative de l’écoulement en sortie de roue est élaborée pour ces calculs. Les résultats de simulation sont comparés avec des mesures expérimentales afin d’évaluer la capacité prédictive de chaque approche de modélisation de la turbulence (URANS et LES). Cette étape de validation met en évidence l’importance d’une définition correcte des trois composantes de la vitesse en entrée d’aspirateur. L’influence des conditions aux limites du domaine de calcul, à savoir la rugosité de la paroi et la condition de sortie de l’aspirateur, sur les résultats de simulation est évaluée, notamment dans le cas d’une résolution LES. Grâce à une analyse détaillée du bilan d’énergie cinétique moyenne dans l’aspirateur, les phénomènes hydrodynamiques responsables des pertes de charge sont identifiés. Ceci permet d'analyser en détail les différences de prédiction de pertes de charge entre les calculs URANS et LES et d’identifier les pistes d’amélioration de la prédiction numérique de ces pertes. Enfin, cette analyse permet de comprendre l’évolution des pertes de charge observée entre plusieurs points de fonctionnement de la turbine. / The draft tube of a hydraulic turbine is the turbine element located downstream of the runner. It has a divergent shape in order to convert the residual kinetic energy leaving the runner into pressure and thus increase the effective head of the turbine. The performances of low head bulb turbines are highly influenced by the head losses in the draft tube. The prediction of these head losses in a design process is thereby a major issue. The numerical prediction of the head losses in the draft tube is a real challenge because the flow in the draft tube is dynamically complex with high Reynolds numbers, a swirl and an adverse pressure gradient. These characteristics render conventional industrial approaches not appropriate. The objective of this work is twofold: (i) to improve the numerical prediction of the turbulent flow in the draft tube by using URANS and LES unsteady approaches and paying special attention to the description of the inlet boundary conditions of the draft tube and (ii) to conduct a detailed analysis of the energy transfers in the draft tube in order to better understand the origin of the head losses. An unsteady inlet boundary condition for the simulations reproducing the flow field at the runner outlet is developed. Numerical results are compared to experimental measurements in order to evaluate the predictive capacity of each turbulence modelling approach (URANS and LES). This validation step highlights the importance of defining properly the three velocity components at the draft tube inlet. The influence on the numerical results of boundary conditions of the calculation domain, such as wall roughness and the outlet boundary condition, is evaluated, in particular in case of LES. Thanks to a detailed analysis of the mean kinetic energy balance in the draft tube, the hydrodynamic phenomena responsible for head losses are identified. The head losses prediction differences between URANS and LES are thus analyzed in detail and possible improvements for the head losses prediction are identified. Finally, this analysis enables to understand the head losses evolution observed between several operating points of the turbine.

Une particularité de la modalité sensorielle olfactive est la nature complexe du stimulus chimique à représenter. Les cellules sensorielles de la cavité nasale sont sensibles aux traits physico-chimiques des molécules et transmettent cette information vers le bulbe olfactif, premier relais central de cette modalité. L’organisation des voies de projection vers le bulbe entraîne une spatialisation de l’activité dans cette structure, ce qui constitue un mode de représentation de l’information mais qui n’est pas suffisant à lui seul. Le bulbe olfactif est également marqué par des phénomènes dynamiques prépondérants. Tout d’abord le rythme respiratoire, qui organise temporellement le niveau d’activation de l’appareil sensoriel, ensuite les oscillations des potentiels de champs locaux, et enfin les oscillations sous-liminaires des potentiels de membrane des cellules. Ces éléments dynamiques pourraient être le support de la formation d’assemblées de neurones, sous-populations de cellules synchronisées transitoirement et permettant la représentation de l’information suivant un principe spatio-temporel. Les travaux présentés dans cette thèse sont basés sur l’enregistrement conjoint des activités unitaires des cellules du bulbe, des oscillations des potentiels de champs locaux et de la respiration en réponse à des stimulations olfactives. Nous montrons les relations existant entre les différents phénomènes dynamiques et comment ils permettent d’organiser l’activité des cellules pour aboutir à la formation d’assemblées de neurones fonctionnelles. Nous mettons particulièrement en évidence le rôle central de la respiration dans le fonctionnement intégré du bulbe olfactif. / A striking feature of the olfactory sensory system is its ability to deal with a complex multi-dimensional chemical stimuli. Receptor cells in the nasal cavity are sensitive to specific features of molecules and transmit this information to the olfactory bulb, first relay for olfaction in the central nervous system. Due to the organization of projection pathways to the bulb, afferent information activates the structure in a topographical fashion ; although this may constitute a coding strategy for olfactory information it has proven insufficient, and other strategies must be investigated. Dynamic phenomenons are a preponderant feature of the olfactory bulb. The respiratory rhythm imposes a sinusoidal level of activation to the system, oscillations in local field potentials and subthreshold oscillations in neurons membrane potentials may interact and lead to the transient synchronization of sub-populations of neurons. This particular mechanism, designated as neural assemblies, is in theory a good candidate for the representation of olfactory information. The work presented here is based on conjoint recordings, in anesthetized animals, of unitary activities, oscillations in the LFP and respiration, in response to olfactory stimulation. We show the relationships existing between the various dynamic phenomenons, and hypothesize on their functional roles. We propose that a same mechanism may form different neural assemblies each assuming a specific functional role. The respiratory rhythm acts as a gating system, organizing the formation of successive yet different neural assemblies.

Traditional irrigation was practiced in Ethiopia since time immemorial. Despite this, water productivity in the sector remained low. A survey on the Godino irrigation scheme revealed that farmers used the same amount of water and intervals, regardless of crop species and growth stage. In an effort to improve the water productivity, two traditional irrigation scheduling methods were compared with two scientific methods, using furrow irrigation. The growth performance and tuber yield of potato (cv. Awash) revealed that irrigation scheduling using a neutron probe significantly outperformed the traditional methods, followed by the SWB model Irrigation Calendar. Since the NP method involves high initial cost and skills, the use of the SWB Calendar is suggested as replacement for the traditional methods. SWB is a generic crop growth model that requires parameters specific to each crop, to be determined experimentally before it could be used for irrigation scheduling. It also accurately describes deficit irrigation strategies where water supply is limited. Field trials to evaluate four potato cultivars for growth performance and assimilate partitioning, and onions' critical growth stages to water stress were conducted. Crop-specific parameters were also generated. Potato and onion crops are widely grown at the Godino scheme where water scarcity is a major constraint. These crop-specific parameters were used to calibrate and evaluate SWB model simulations. Results revealed that SWB model simulations for Top dry matter (TDM), Harvestable dry matter (HDM), Leaf area index (LAI), soil water deficit (SWD) and Fractional interception (FI) fitted well with measured data, with a high degree of statistical accuracy. The response of onions to water stress showed that bulb development (70-110 DATP) and bulb maturity (110-145) stages were most critical to water stress, which resulted in a significant reduction in onion growth and bulb yields. SWB also showed that onion yield was most sensitive to water stress during these two stages. An irrigation calendar, using the SWB model, was developed for five different schemes in Ethiopia, using long-term weather data and crop-specific parameters for potatoes and onions. The calendars revealed that water depth varied, depending on climate, crop type and growth stage. / Thesis (PhD)--University of Pretoria, 2009. / Plant Production and Soil Science / unrestricted

Accelerated Bridge Construction (ABC) has gained substantial popularity in new bridge construction and bridge deck replacement because it offers innovative construction techniques that result in time and cost savings when compared to traditional bridge construction practice. One technology commonly implemented in ABC to effectively execute its projects is the use of prefabricated bridge components (precast/prestressed bridge components). Precast/prestressed bridge components are fabricated offsite or near the site and then connected on-site using small volume closure pour connections. Diaphragms are also commonly used to strengthen the connection between certain prefabricated components used in ABC, such as beam elements. Bridges containing closure pour connections and diaphragms can be designed using AASHTO LRFD live-load distribution factor formulas under the condition that the bridge must be sufficiently connected. However, these formulas were developed using analytical models that did not account for the effects of closure pours and diaphragms on live-load distribution. This research study investigates live-load distribution characteristics of precast/prestressed concrete bridges with closure pour connections and diaphragms. The investigation was conducted using finite element bridge models with closure pour joints that were calibrated using experimental data and different configuration of diaphragms. The concrete material used for the closure pour connections was developed as part of a larger project intended to develop high early-strength concrete mixtures that specifically reach strength in only 12 hours, a critical requirement for ABC projects.

Cette thèse s’intéresse à la capacité du cerveau à s’adapter à un environnement changeant. Plus spécifiquement, elle s’intéresse à la plasticité cérébrale dans le système olfactif. Les sommeliers, experts dans le domaine de l’olfaction, ont constitué notre modèle. Une première étude nous a permis d’établir un protocole afin de tester la performance olfactive des sommeliers. Dans une deuxième étude, nous avons testé des étudiants en sommellerie au début de leur formation d’un an et demi qui mène à la profession de sommelier. Nous avons observé que ces futurs experts de l’olfaction présentaient déjà, au cours des deux premiers mois, des capacités olfactives supérieures. Dans une troisième étude, nous avons de nouveau testé les étudiants à la fin de leur formation, afin d’examiner les effets d’un entraînement olfactif à long terme sur la performance olfactive et sur le cerveau : en plus de mesurer les capacités olfactives avec le test des Sniffin’ Sticks, nous avons utilisé l’imagerie par résonance magnétique (IRM) pour évaluer l’évolution du cerveau au cours de la formation en sommellerie. Nos principales observations concernent des changements au niveau de la structure cérébrale. Premièrement, le volume du bulbe olfactif a augmenté au cours de la formation, ce qui est en accord avec la littérature disponible à propos de cette structure. Deuxièmement, nous avons observé un épaississement au niveau du cortex entorhinal mais aussi un amincissement au niveau d’autres régions du cortex. Mises en relation avec les résultats d’études antérieures, ces observations soutiennent le récent modèle de surproduction-élagage selon lequel les changements dus à la plasticité liée à l’entraînement ne sont pas linéaires mais font intervenir différents processus en plusieurs phases. Ce modèle constitue une avancée importante dans la compréhension des mécanismes impliqués dans la plasticité cérébrale et devrait être pris en compte dans les futures études sur la plasticité. Bien que les résultats sur le plan neuroimagerie soient intéressants, les résultats de l’étude longitudinale relatifs à la performance olfactive n’étaient pas concluants sur le plan comportemental. Nous avons donc mis en place dans une quatrième étude une tâche d’identification d’odorants au sein de mélanges plus complexe et plus adaptée aux sommeliers qui a confirmé la supériorité de leurs capacités olfactives. Nous avons aussi entraîné des novices sur cette tâche pendant cinq jours pour tester les effets d’un court entraînement olfactif. Cette thèse est organisée sous forme de thèse par articles. Le premier chapitre correspond à l’introduction générale, qui est elle-même organisée en plusieurs grandes parties. Ces différentes parties définissent les concepts-clés de cette thèse : l’olfaction, les corrélations neuroanatomiques dans le système olfactif, la plasticité cérébrale, la plasticité liée à l’entraînement dans le système olfactif, la neuroimagerie. La dernière partie conclut l’introduction en présentant les objectifs et hypothèses de recherche. Les chapitres suivants correspondent aux articles rédigés au cours du doctorat et présentant les résultats des recherches. Le dernier chapitre constitue une discussion générale. Enfin, en annexes se trouvent deux articles publiés lors du doctorat, un chapitre à paraître dans un livre ainsi que des résultats non publiés. / This thesis is about the brain’s ability to adapt to an ever-changing environment. More specifically, it is about brain plasticity in the olfactory system. We used sommeliers, who are experts in olfaction, as our model. A first study allowed us to instate a protocol to assess sommeliers’ olfactory function. In a second study, we tested sommelier students at the start of their year-and-a-half-long training which is the prerequisite to become a professional sommelier. We observed that these future experts in olfaction already had, during the first two months of training, superior olfactory abilities. In a third study, we tested sommelier students again at the end of their training to examine the effects of a long-term olfactory training on olfactory performance and on the brain: beside assessing olfactory performance with the Sniffin’ Sticks test, we used magnetic resonance imaging (MRI) to examine the evolution of brain structure and function during sommelier training. Changes in brain structure constituted our main results. Firstly, olfactory bulb volume increased during sommelier training, which is in line with previous reports about this structure. Secondly, we observed a cortical thickness increase in the entorhinal cortex but also cortical thinning in other brain areas. Put together with findings from previous studies, these results support the recent overproduction-pruning model of plasticity according to which changes due to training-related brain plasticity are nonlinear but involve different processes and different phases. This model constitutes a great advance in the understanding of brain plasticity and its underlying mechanisms and should be considered in future studies about plasticity. Though neuroimaging results were interesting, results from olfactory tests in our longitudinal study were not conclusive so we conducted a fourth study to test the ability to identify odorants within mixtures, a task which is more complex and suitable for sommeliers than the Sniffin’ Sticks test. Sommeliers performed better. We also tested novices that we had trained on this task for five days to evaluate the effects of a short-term olfactory training. This thesis is organized by articles. The first chapter is a general introduction, itself organized in several parts. These different parts define the major concepts of this thesis: olfaction, neuroanatomical correlations in the olfactory system, brain plasticity, plasticity in the olfactory system, neuroimaging. The last part concludes the introduction with aims and hypotheses. The following chapters are articles written during PhD that present the results of our research. The last chapter is a general discussion of all the results. Finally, two articles published during PhD, a chapter that is to be published in a book and unpublished results are presented as appendices.