A predição do desempenho de touros na produção in vitro de embriões (PIVE), pela avaliação seminal, não está plenamente estabelecida. Com este estudo, objetivou-se caracterizar as diferenças nos atributos espermáticos de touros com baixo e alto desempenho, baseado nas taxas de clivagem e de blastocisto, visando estabelecer métodos preditivos de maior eficiência na PIVE. A partir do histórico de três anos da empresa In Vitro Brasil®, foram selecionados touros que haviam resultado nas menores e maiores taxas de clivagem e de blastocisto, estabelecendo-se 4 grupos experimentais: baixa taxa de clivagem (BC, n=5), alta taxa de clivagem (AC, n=5), baixa taxa de blastocisto (BB, n=5) e alta taxa de blastocisto (AB, n=5). Foram adquiridas palhetas de sêmen congelado dos touros de cada grupo, nas quais foram avaliados, antes (Pré-Percoll®) e após centrifugação em gradiente de Percoll® (Pós-Percoll®), os atributos espermáticos: cinética, em sistema CASA; integridade de membranas plasmática e acrossomal (FITC-PSA/PI), potencial de membrana mitocondrial (JC-1), status oxidativo (DHE, CellROXTM green/PI, MitoSox red), apoptose (FITC-FMK-VAD/PI) e DNA (SCSA), por citometria de fluxo; morfologia; atividade mitocondrial (DAB); capacitação (CTC); teste funcional e TBARS induzido. Diferenças entre grupos (BC vs. AC e BB vs. AB) foram estimadas pelo teste t. Para todas as análises estatísticas foi considerado nível de significância de 5%. Na ausência de interações significativas, o efeito dos grupos foi analisado ao fundir os momentos Pré e Pós-Percoll®. Os grupos AC e AB apresentaram valores superiores de VAP, VCL, ALH e DNC e valores inferiores de BCF, STR, LIN e WOB, cinética indicativa de hiperativação. Adicionalmente, estes grupos apresentaram indícios de menor ocorrência de estresse oxidativo, evidenciados pelo percentual de espermatozoides com membrana lesada e estresse oxidativo (CellROXTM green/PI) no grupo AC e nível de DHE no grupo AB. Para os demais atributos estudados, não se obteve diferença entre os grupos. Por meio de análise de regressão logística multivariada, constatou-se que a taxa de clivagem sofre efeito de: VCL, BCF e percentual de espermatozoides com integridade de membrana e sem estresse oxidativo; já a taxa de blastocisto, sofre efeito de: defeitos menores, VSL, DABIII e DHE, os quais foram inclusos nos respectivos modelos preditivos. As taxas obtidas com o modelo preditivo foram comparadas às taxas reais por análise de correlação de Pearson, que resultaram em intensidade moderada tanto para clivagem (r=0,56) quanto para blastocisto (r=0,44). Subpopulações espermáticas com diferentes perfis de cinética em CASA, foram determinadas em três etapas estatísticas segundo Núñez-Martínez et al. (2006). Foram detectadas 4 subpopulações: 1-Rápidos e progressivos; 2- Hiperativados; 3- Lentos e sinuosos; 4- Lentos e progressivos. Touros com alto desempenho (AC e AB) apresentaram maior percentual de subpopulação 2, Pré-Percoll®, e das subpopulações 1 e 2, Pós-Percoll®. Com isto, demonstra-se ser possível diferenciar touros de baixo e alto desempenho na PIVE, pela avaliação dos atributos espermáticos, com o estabelecimento de modelos preditivos para as taxas de clivagem e de blastocisto. Ademais, o estudo das subpopulações espermáticas constitui-se no método mais efetivo para a predição do desempenho in vitro. / The prediction of bull performance on the in vitro production (IVP) of bovine embryos, by the seminal evaluation, is not fully established. Thus, the aim of this study was to characterize the differences in the sperm features of bulls with low and high performance, based on cleavage and blastocyst rates, in order to establish predictive methods for a greater efficiency in the IVP. Based on the results obtained in three years from the company In Vitro Brasil®, bulls were selected by the lowest and highest cleavage and blastocyst rates, establishing 4 experimental groups: low cleavage rate (BC, n=5), high cleavage rate (AC, n=5), low blastocyst rate (BB, n=5) and high blastocyst rate (AB, n=5). Frozen semen were obtained from the bulls of each group, in which were evaluated, before (Pre-Percoll®) and after Percoll® gradient centrifugation (Post-Percoll®), the sperm features: kinetics, in CASA; integrity of plasma and acrosomal membranes (FITC-PSA/PI), mitochondrial membrane potential (JC-1), oxidative status (DHE, CellROXTM green/PI, MitoSox red), apoptosis (FITC-FMK-VAD/PI) and DNA (SCSA), by flow cytometry; morphology; mitochondrial activity (DAB); capacitation (CTC); functional test and induced TBARS. Differences between groups (BC vs. AC and BB vs. AB) were estimated by t test. A significance level of 5% was considered for all statistical analysis. In the absence of significant interactions, the effect of the groups was analyzed by merging the moments Pre and Post-Percoll®. The AC and AB groups presented higher values of VAP, VCL, ALH and DNC and lower values of BCF, STR, LIN and WOB, kinetics indicative of hyperactivation. In addition, these groups showed less evidence of oxidative stress, observed by the percentage of spermatozoa with damaged membrane and oxidative stress (CellROXTM green/PI) in the AC group and level of DHE in the AB group. For the others sperm features, there was no difference between groups. By multivariate logistic regression analysis, it was verified that the cleavage rate is affected by: VSL, BCF and percentage of spermatozoa with membrane integrity and without oxidative stress; and the blastocyst rate, is affected by: minor defects, VSL, DABIII and DHE, which were included in the respective predictive models. The rates obtained with the predictive model were compared to the real rates by Pearson correlation analysis, which resulted in moderate intensity for both cleavage (r=0.56) and blastocyst (r=0.44). Spermatic subpopulations with different kinetic profiles at CASA were determined in three statistical steps according to Núñez-Martínez et al. (2006). Four subpopulations were detected: 1-Fast and progressive; 2- Hyperactivated; 3- Slow and sinuous; 4- Slow and progressive. Bulls with high performance (AC and AB) presented higher percentage of subpopulation 2, Pre-Percoll®, and subpopulations 1 and 2, Post-Percoll®. In summary, it is possible to differentiate low and high-performance bulls at IVP by evaluating the sperm features, with the establishment of predictive models for the cleavage and blastocyst rates. Furthermore, the study of sperm subpopulations is the most effective method for predicting in vitro performance.

A predição do desempenho de touros na produção in vitro de embriões (PIVE), pela avaliação seminal, não está plenamente estabelecida. Com este estudo, objetivou-se caracterizar as diferenças nos atributos espermáticos de touros com baixo e alto desempenho, baseado nas taxas de clivagem e de blastocisto, visando estabelecer métodos preditivos de maior eficiência na PIVE. A partir do histórico de três anos da empresa In Vitro Brasil®, foram selecionados touros que haviam resultado nas menores e maiores taxas de clivagem e de blastocisto, estabelecendo-se 4 grupos experimentais: baixa taxa de clivagem (BC, n=5), alta taxa de clivagem (AC, n=5), baixa taxa de blastocisto (BB, n=5) e alta taxa de blastocisto (AB, n=5). Foram adquiridas palhetas de sêmen congelado dos touros de cada grupo, nas quais foram avaliados, antes (Pré-Percoll®) e após centrifugação em gradiente de Percoll® (Pós-Percoll®), os atributos espermáticos: cinética, em sistema CASA; integridade de membranas plasmática e acrossomal (FITC-PSA/PI), potencial de membrana mitocondrial (JC-1), status oxidativo (DHE, CellROXTM green/PI, MitoSox red), apoptose (FITC-FMK-VAD/PI) e DNA (SCSA), por citometria de fluxo; morfologia; atividade mitocondrial (DAB); capacitação (CTC); teste funcional e TBARS induzido. Diferenças entre grupos (BC vs. AC e BB vs. AB) foram estimadas pelo teste t. Para todas as análises estatísticas foi considerado nível de significância de 5%. Na ausência de interações significativas, o efeito dos grupos foi analisado ao fundir os momentos Pré e Pós-Percoll®. Os grupos AC e AB apresentaram valores superiores de VAP, VCL, ALH e DNC e valores inferiores de BCF, STR, LIN e WOB, cinética indicativa de hiperativação. Adicionalmente, estes grupos apresentaram indícios de menor ocorrência de estresse oxidativo, evidenciados pelo percentual de espermatozoides com membrana lesada e estresse oxidativo (CellROXTM green/PI) no grupo AC e nível de DHE no grupo AB. Para os demais atributos estudados, não se obteve diferença entre os grupos. Por meio de análise de regressão logística multivariada, constatou-se que a taxa de clivagem sofre efeito de: VCL, BCF e percentual de espermatozoides com integridade de membrana e sem estresse oxidativo; já a taxa de blastocisto, sofre efeito de: defeitos menores, VSL, DABIII e DHE, os quais foram inclusos nos respectivos modelos preditivos. As taxas obtidas com o modelo preditivo foram comparadas às taxas reais por análise de correlação de Pearson, que resultaram em intensidade moderada tanto para clivagem (r=0,56) quanto para blastocisto (r=0,44). Subpopulações espermáticas com diferentes perfis de cinética em CASA, foram determinadas em três etapas estatísticas segundo Núñez-Martínez et al. (2006). Foram detectadas 4 subpopulações: 1-Rápidos e progressivos; 2- Hiperativados; 3- Lentos e sinuosos; 4- Lentos e progressivos. Touros com alto desempenho (AC e AB) apresentaram maior percentual de subpopulação 2, Pré-Percoll®, e das subpopulações 1 e 2, Pós-Percoll®. Com isto, demonstra-se ser possível diferenciar touros de baixo e alto desempenho na PIVE, pela avaliação dos atributos espermáticos, com o estabelecimento de modelos preditivos para as taxas de clivagem e de blastocisto. Ademais, o estudo das subpopulações espermáticas constitui-se no método mais efetivo para a predição do desempenho in vitro. / The prediction of bull performance on the in vitro production (IVP) of bovine embryos, by the seminal evaluation, is not fully established. Thus, the aim of this study was to characterize the differences in the sperm features of bulls with low and high performance, based on cleavage and blastocyst rates, in order to establish predictive methods for a greater efficiency in the IVP. Based on the results obtained in three years from the company In Vitro Brasil®, bulls were selected by the lowest and highest cleavage and blastocyst rates, establishing 4 experimental groups: low cleavage rate (BC, n=5), high cleavage rate (AC, n=5), low blastocyst rate (BB, n=5) and high blastocyst rate (AB, n=5). Frozen semen were obtained from the bulls of each group, in which were evaluated, before (Pre-Percoll®) and after Percoll® gradient centrifugation (Post-Percoll®), the sperm features: kinetics, in CASA; integrity of plasma and acrosomal membranes (FITC-PSA/PI), mitochondrial membrane potential (JC-1), oxidative status (DHE, CellROXTM green/PI, MitoSox red), apoptosis (FITC-FMK-VAD/PI) and DNA (SCSA), by flow cytometry; morphology; mitochondrial activity (DAB); capacitation (CTC); functional test and induced TBARS. Differences between groups (BC vs. AC and BB vs. AB) were estimated by t test. A significance level of 5% was considered for all statistical analysis. In the absence of significant interactions, the effect of the groups was analyzed by merging the moments Pre and Post-Percoll®. The AC and AB groups presented higher values of VAP, VCL, ALH and DNC and lower values of BCF, STR, LIN and WOB, kinetics indicative of hyperactivation. In addition, these groups showed less evidence of oxidative stress, observed by the percentage of spermatozoa with damaged membrane and oxidative stress (CellROXTM green/PI) in the AC group and level of DHE in the AB group. For the others sperm features, there was no difference between groups. By multivariate logistic regression analysis, it was verified that the cleavage rate is affected by: VSL, BCF and percentage of spermatozoa with membrane integrity and without oxidative stress; and the blastocyst rate, is affected by: minor defects, VSL, DABIII and DHE, which were included in the respective predictive models. The rates obtained with the predictive model were compared to the real rates by Pearson correlation analysis, which resulted in moderate intensity for both cleavage (r=0.56) and blastocyst (r=0.44). Spermatic subpopulations with different kinetic profiles at CASA were determined in three statistical steps according to Núñez-Martínez et al. (2006). Four subpopulations were detected: 1-Fast and progressive; 2- Hyperactivated; 3- Slow and sinuous; 4- Slow and progressive. Bulls with high performance (AC and AB) presented higher percentage of subpopulation 2, Pre-Percoll®, and subpopulations 1 and 2, Post-Percoll®. In summary, it is possible to differentiate low and high-performance bulls at IVP by evaluating the sperm features, with the establishment of predictive models for the cleavage and blastocyst rates. Furthermore, the study of sperm subpopulations is the most effective method for predicting in vitro performance.

Detailed analysis of the physical, chemical, pollen and diatom stratigraphy of sediment cores from Bull Run Lake, Oregon reveals that the lake has been subject to repeated episodes of disruption by volcanic ash fall, fire and other disturbance over the past 4300 years. With the exception of fire, these disruptions have not resulted in long term changes in the watershed or in the characteristics of Bull Run Lake. A major fire in the watershed about 750 years ago altered the vegetation. Since that time the forest has been returning to its former condition.

Violin virtuosos Henryk Wieniawski, Ole Bull, and Pablo de Sarasate each composed short virtuosic works based on their own cultural heritage. This thesis examines the exotic elements incorporated into the character pieces by the three violinists. It draws upon contemporary literature and newspaper reviews of their performances in order to demonstrate the ways in which the violinists and their music were perceived as representative of nineteenth-century exoticism. Wieniawski, whose musical training was primarily French, produced exotic Polish polonaises and mazurkas, which were perceived as evidence of Polish national character, but only in his homeland of Poland. In contrast, Ole Bull's Norwegian heritage was central to his professional persona. His compositions were influenced by Norwegian fiddling and fiddle tunes as well as the pastoral conventions of European art music. Sarasate drew on music from a wide variety of geographical and cultural regions within Spain in his Spanish dances. While his dances were extrememly popular with audiences, critical reception was often dismissive. The individual personas and international receptions of Wieniawski, Bull, and Sarasate were shaped by the musical characteristics of their homelands heard in their works.

Consumption of toxic endophyte-infected (E+) tall fescue pastures is known to have a negative impact on bull reproductive performance. Since decreased cleavage rates of embryos fertilized with spermatozoa from bulls grazing E+ tall fescue pastures have been observed in several studies using differing sets of bulls, technicians, pastures, and other methods of inducing tall fescue toxicosis (ergotamine tartrate), it is hypothesized that spermatozoa function from bulls grazing E+ is impaired in ways undetectable by gross semen examination. During a three-month grazing study, 6 Angus bulls were utilized to determine the effects of grazing E+ tall fescue pastures on growth performance and spermatozoa function. Bulls were appointed to graze Kentucky 31 tall fescue (Festuca arundinacea Schreb.) infected with Neotyphodium coenophialum, an ergot alkaloid producing endophyte (n=3) or Jesup tall fescue infected with non-ergot alkaloid producing endophyte (NTE) MaxQTM (n=3). Bulls were grouped by body weight (BW) and scrotal circumference (SC) to graze pastures from April 18-June 26, 2007. Blood samples, BW, SC, semen, and rectal temperatures (RT) were collected every 7 d. Scrotal temperatures (ST) were obtained before semen collection each week in June. Semen was evaluated for gross motility, morphology, and Computer Assisted Semen Analysis (CASA) parameters. Semen from a subset of bulls (n=2 per treatment) was used to assess spermatozoa ability to function utilizing in vitro assays. Growth performance was decreased in E+ bulls compared to bulls grazing NTE tall fescue pastures (P = 0.002). Concentrations of prolactin were reduced in bulls grazing E+ compared to bulls grazing NTE tall fescue pastures (P = 0.055). Motility post-thaw and during a 3-hour stress test were decreased (P = 0.024 and P < 0.0001, respectively), in addition to altered CASA parameters for spermatozoa. Penetration was reduced in oocytes fertilized with spermatozoa from bulls grazing E+ (64.54 ± 3.28%) compared to NTE tall fescue pastures (87.42 ± 1.63%, P < 0.0001) coupled with hastened meiotic completion, and reduced intracellular calcium parameters. These findings indicate impaired spermatozoa function in bulls grazing E+ tall fescue pastures that extends beyond gross semen characteristics, and may provide direction for future studies.

A series of experiments was conducted to evaluate the effects of nutrition during calfhood (defined as the period from 10 to 26-30 wk of age) and peripubertal period (defined as the period from 27-31 to 70-74 wk of age) on sexual development and reproductive function in beef bulls. The overall objective of these experiments was to evaluate the effects of nutrition on endogenous metabolic hormones (leptin, insulin, GH, and IGF-I), gonadotropins and testosterone concentrations, sexual development, sperm production, and semen quality in bulls. The results of these experiments demonstrated that nutrition affected GnRH secretion and sexual development in bulls. Increased nutrition during calfhood resulted in a more sustained increase in LH pulse frequency during the early gonadotropin rise and greater testicular development at maturity. On the other hand, low nutrition during calfhood suppressed LH secretion during the early gonadotropin rise and resulted in delayed puberty and reduced testicular development at maturity. When low nutrition was accomplished by restricted feed intake, hypothalamic and pituitary function were compromised and LH secretion was more severely affected. Temporal associations between LH secretion patterns and circulating IGF-I concentrations implied that IGF-I is a possible signal to the central metabolic sensor involved in translating body nutritional status to the GnRH pulse generator. Nutrition also affected testicular steroidogenesis (testosterone concentrations), indicating effects on the number or function of Leydig cells, or both. Age-related increases in physiological and GnRH-stimulated circulating testosterone concentrations were hastened in bulls receiving high nutrition and delayed in bulls receiving low nutrition; these effects were probably mediated by both LH secretion and IGF-I concentrations. Circulating leptin and insulin may have only permissive roles on GnRH secretion, but may enhance testicular development. Growth hormone concentrations decreased concomitantly with increasing IGF-I concentrations during sexual development in bulls, suggesting that the testes could contribute considerable amounts of circulating IGF-I. In conclusion, management strategies to optimize reproductive function in bulls should focus on increasing nutrition during calfhood.

A series of experiments was conducted to evaluate the effects of nutrition during calfhood (defined as the period from 10 to 26-30 wk of age) and peripubertal period (defined as the period from 27-31 to 70-74 wk of age) on sexual development and reproductive function in beef bulls. The overall objective of these experiments was to evaluate the effects of nutrition on endogenous metabolic hormones (leptin, insulin, GH, and IGF-I), gonadotropins and testosterone concentrations, sexual development, sperm production, and semen quality in bulls. The results of these experiments demonstrated that nutrition affected GnRH secretion and sexual development in bulls. Increased nutrition during calfhood resulted in a more sustained increase in LH pulse frequency during the early gonadotropin rise and greater testicular development at maturity. On the other hand, low nutrition during calfhood suppressed LH secretion during the early gonadotropin rise and resulted in delayed puberty and reduced testicular development at maturity. When low nutrition was accomplished by restricted feed intake, hypothalamic and pituitary function were compromised and LH secretion was more severely affected. Temporal associations between LH secretion patterns and circulating IGF-I concentrations implied that IGF-I is a possible signal to the central metabolic sensor involved in translating body nutritional status to the GnRH pulse generator. Nutrition also affected testicular steroidogenesis (testosterone concentrations), indicating effects on the number or function of Leydig cells, or both. Age-related increases in physiological and GnRH-stimulated circulating testosterone concentrations were hastened in bulls receiving high nutrition and delayed in bulls receiving low nutrition; these effects were probably mediated by both LH secretion and IGF-I concentrations. Circulating leptin and insulin may have only permissive roles on GnRH secretion, but may enhance testicular development. Growth hormone concentrations decreased concomitantly with increasing IGF-I concentrations during sexual development in bulls, suggesting that the testes could contribute considerable amounts of circulating IGF-I. In conclusion, management strategies to optimize reproductive function in bulls should focus on increasing nutrition during calfhood.

The purpose of this thesis aims that common investors are expected to apply three kinds of active portfolio management without complex mathematical numeration to reduce investment risk or loss to get profits on Taiwan stock exchange capitalization weighted stock index options market. They are Constant Portion Portfolio Insurance Strategy (named CPPI), Time-Invariant Portfolio Protection (named TIPP) and Constant Mix Strategy (named CMS). The active managements adopt bull and bear spread as an adjustment of risk positions on the option market. Five research results are as followings: 1. The performance of the portfolio managements as Constant Portion Portfolio Insurance Strategy and Time - Invariant Portfolio Protection is better than that of the management as Constant Mix Strategy. And Floor value of the portfolio management as Time - Invariant Portfolio Protection exceeds that of the portfolio management as Constant Portion Portfolio Insurance Strategy. 2. Due to the regulation of margin system by Taiwan Future Exchange, the positions of Bull Call Spread and Bear Put Spread can be greater than ones of Bull Put Spread and Bear Call Spread. 3. The more differences between two strike prices on the spread option means to be able to get more profit opportunities. 4. Greater multiplier number and profit have inverse relationship. That only occurs on portfolio managements in Constant Portion Portfolio Insurance Strategy and Time - Invariant Portfolio Protection. 5. Setting upper and low limitation of single return rate for offsetting will have better performance than no limitations for the managements in Constant Portion Portfolio Insurance Strategy and Time - Invariant Portfolio Protection.

A very high proportion of beef cows in Australia are mated to bulls at pasture. A multiplicity of systems for pasture mating are presently in use. Bulls are mated singly or in groups, they are set-stocked during the mating period or rotated about the different groups of cows, they are mated at the rate of 2, 3, 4, 5 or 6 bulls per 100 cows, young bulls are mated to cows in company with old bulls or other young bulls (Dickson 1966). There is little scientific basis for any of these mating systems since basic information on the sexual and social behaviour of beef bulls at pasture is lacking. Such data are needed so that optimum use can be made of beef bulls in pasture mating. (For complete abstract open document)

This thesis take the form of an ethnographic exploration of a bull-breeding estate called Partido de Resina (formerly Pablo Romero) in the countryside near Seville in Andalusia. The estate, founded in 1885, produces fighting bulls for taurine events in Southern France, Spain and Portugal. At the heart of the thesis is the life cycle of the fighting animals, every chapter being anchored to a particular point in the bull-breeding calendar and the lives of the stock. Each chapter draws out specific qualities of the world of the bulls from the perspective of Partido de Resina, rooting the bulls and their people in a wider Spanish and Andalusian landscape and history, with a focus on technical know-how and everyday ethics after the 2008 financial crisis. The professionals who care for the Partido de Resina bulls, cows, and calves are the human protagonists of this project; their working routines, hopes, concerns, and stories described through their interactions with the animals which they look after. The core anthropological argument in the thesis is to show how different ethnographically salient forms of life emerge on and around the estate, sometimes weighted towards individual animals, sometimes towards bits of taurine bodies, or breeds, types, lineages, cohorts, and other groupings of stock. The varied, dynamic presence of animal life is contextualised in the literature of the 'animal turn' in anthropology, which has drawn non- human life into the ethnographic foreground. A case is made for a nuanced and contextual ethnographic attention to animal life and interiority as it emerges in the field, without an a priori emphasis on animal personhood or subjectivity. In foregrounding the qualities and concerns encountered and worked through during both routine livestock maintenance and extraordinary, definitive events like bullfights, the emergent, multiple character of taurine forms of existence become apparent.