Um dos tipos de câncer mais frequentes no mundo é o carcinoma epidermoide de cabeça e pescoço. A dificuldade de resposta eficiente aos tratamentos está relacionada à complexidade molecular deste tumor. MicroRNAs regulam uma parte significativa dos genes humanos, dentre os quais genes responsáveis por controlar proliferação e apoptose, caracterizando seu papel na progressão tumoral e resistência a quimioterápicos. O entendimento sobre como moléculas com ação antineoplásica atuam sobre a regulação de microRNAs pode contribuir para a compreensão de resultados terapêuticos. Neste estudo avaliamos o efeito dos triterpenos quinonametídeos maitenina e 22-β-hidroximaitenina sobre a viabilidade celular e sobre a regulação de microRNAs associados com proliferação e apoptose. O efeito destas moléculas foi avaliado frente a linhagens tumorais e em queratinócitos orais livres de tumor. Os resultados indicam que os dois triterpenos avaliados reduzem eficazmente a viabilidade das células cancerígenas e a regulação de microRNAs faz parte dos mecanismos envolvidos neste efeito. / Head and neck squamous cell carcinoma is one of the most frequent cancer types in the world. The difficulty of effective response to treatment is related to the molecular complexity of this tumor. MicroRNAs, small non-coding RNA molecules, regulate a significant proportion of human genes, among which genes involved in cell growth, apoptosis and response to chemotherapy. The comprehension on how anticancer molecules interfere with microRNA expression should contribute to the understanding of treatment outcome. This study evaluated the effect of two quinonamethide triterpenoids, maytenin and 22-β-hidroxymaytenin, on cell viability and on the regulation of microRNAs associated with proliferation and apoptosis. The effect of these molecules was evaluated in tumor cells and oral keratinocytes. The results show that the two triterpenoids effectively reduced cell viability of cancer-derived cell lines and that microRNA regulation is one of the mechanisms involved in this effect.

Mobile communication has become an essential part of our daily life. We love the flexibility of wireless cell phones and even accept their lower quality of service when compared to wired links. Similarly, we are looking forward to the day that we can continue watching our favorite TV programs while travelling anywhere and everywhere. Mobility, flexibility, and portability are the themes of the next generation communication. Motivated and fascinated by such technology breakthroughs, this effort is geared towards enhancing the quality of wireless services and bringing mobile satellite reception one step closer to the market. Meanwhile, phased array antennas are vital components for RADAR applications where the antenna is required to have certain scan capabilities. One of the main concerns in that perspective is how to avoid the potential of scan blindness in the required scan range. Targeting to achieve wide-band wide-scan angle phased arrays free from any scan blindness our efforts is also geared. Conventionally, the key to lower the profile of the antenna is to use planar structures. In that perspective microstrip patch antennas have drawn the attention of antenna engineers since the 1970s due to their attractive features of being low profile, compact size, light weight, and amenable to low-cost PCB fabrication processes. However, patch elements are basically resonating at a single frequency, typically have <2% bandwidth, which is a major deficit that impedes their usage in relatively wide-band applications. There are various approaches to enhance the patch antennas bandwidth including suspended substrates, multi-stack patches, and metalized cavities backing these patches. Metalized cavity-backed patch structures have been demonstrated to give the best performance, however, they are very expensive to manufacture. In this dissertation, we develop an alternative low-cost bandwidth enhancement topology. The proposed topology is based on substrate-integrated waveguides. The great potential of the proposed structure lies in being amenable to the conventional PCB fabrication. Moreover, substrate-integrated cavity-backed structures facilitate the design of sophisticated arrays that are very expensive to develop using the conventional metalized cavity-backed topology, which includes the common broadside arrays used in fixed-beam applications and the scanned phased arrays used in RADAR applications.

Vertical-cavity lasers (VCLs) are of great interest as lightsources for fiber-optic communication systems. Such deviceshave a number of advantages over traditional in-plane laserdiodes, including low power consumption, efficient fibercoupling, on-chip testability, as well as potential low-costfabrication and packaging. To date, GaAs-based VCLs operatingat 850 nm are the technology of choice for short-distance,high-speed data transmission over multimode fiber. Forlong-distance communication networks, long-wavelength (LW) VCLsoperating in the 1.3 and 1.55-&amp;#956m transmission windowsof standard singlemode fibers are desired. However, despiteconsiderable worldwide development efforts, the commercialbreakthrough of such devices has still to be achieved. This ismainly due to shortcomings of the intrinsic material propertiesof InP-based material systems, traditionally employed in LWlaser diodes. While LW quantum well (QW) active regions basedon InP are well established, efficient distributed Braggreflectors (DBRs) are better built up in the AlGaAs/GaAsmaterial system. Therefore, earlier work on LW VCLs has focusedon hybrid techniques such as bonding between InP-based QWs andAlGaAs/GaAs DBRs using waferfusion. More recently, however, themain interest in this field has shifted towards all-epitaxialGaAs-based devices employing novel 1.3-&amp;#956m activematerials with strained GaInNAs QWs as one of the mostpromising candidates. The main focus of this thesis is on the characterization andanalysis of LW VCLs and building blocks thereof, based on bothInP and GaAs substrates. This includes a theoretical study on1.3-&amp;#956m InGaAsP/InP multiple QW active regions, as wellas an experimental investigation of novel, highly strained1.2-&amp;#956m InGaAs/GaAs single QWs. Two high-accuracyabsolute reflectance measurement setups were built for thecharacterization of various DBRs. Reflectance measurementsrevealed that n-type doping is much more detrimental to theperformance of AlGaAs/GaAs DBRs than previously anticipated.Near-room temperature operation of a single-fused1.55-&amp;#956m VCL with an InP/InGaAsP bottom DBR wasobtained. A thermal analysis of this device structure clearlyindicated its limited capabilities in terms of high-temperatureoperation. As a result, further efforts were directed towardsall-epitaxial GaAs-based VCLs. Record-long emission wavelengthsto above 1260 nm were obtained from InGaAs VCLs based on anextensive gain–cavity detuning. These devices showed verypromising performance characteristics in terms of thresholdcurrent and light output power, indicating good potential forbeing a viable alternative to GaInNAs-based VCLs.

Electroabsorption is the physical phenomenon by which the absorption of light in a medium can be controlled by applying an electric field. The Quantum–Confined Stark Effect, which makes the absorption band–edge in quantum wells very field–dependent, together with the strong absorption peak provided by excitons, are the physical foundations for the success of electroabsorption modulators based on quantum well structures in telecommunication networks. This thesis describes the design and fabrication of surface–normal electroabsorption modulation devices. The techniques needed to understand the design and fabrication of surface–normal multiple quantum well optical modulators are introduced, as are the various characterisation techniques used during and after the fabrication. Devices for several types of applications have been designed, fabricated, characterised and in some cases integrated into optical systems: – Two–dimensional arrays of 128´128 pixel amplitude modulators grown on GaAs substrates have been fabricated and characterised. Speeds of up to 11700 frames per second were demonstrated, limited by the output electronics of the computer interface. – Large–area modulators grown on GaAs substrates for free–space optical communication were developed, with an active area of 2cm2 and a modulation speed of several megahertz. Contrast ratios up to 5:1 on full modulator areas were measured. Problems limiting the yield and modulation speed of such devices have been studied, and solutions to overcome them have been demonstrated. – Large–area devices grown on InP substrates for free–space optical communication have been developed. Contrast ratios of up to 2:1 for transmissive types have been demonstrated. – Devices consisting of two rows of pixels, grown on GaAs substrates, with an active area of 22mm´5mm, divided into 64 or 128 pixels per row have been developed. These amplitude modulation devices were designed for optical signal processing applications. – One variant of these optical signal processing devices was also characterised as a ternary, binary amplitude and binary phase modulator array. – The use of GaAs multiple quantum well optical modulators in a free–space optical retro–communication system has been studied. An opto–mechanical design for a modulating retro–reflector is described, allowing a large field of view in one direction using reflecting, resonant–cavity modulators for high contrast ratios. / QC 20100802

Fiber loop ring down (FLRD) spectroscopy is a sensitive spectroscopic technique that is based on absorption and it is convenient for trace chemical detection. Different FLRD systems are being improved in order to increase their sensitivity. In FLRD spectroscopy, detection of a sample is done by measuring of a leaking light at each trip within an optical cavity. Intensity of leaking light has an exponential decay where it is reduced by absorption of sample and scattering of light. In this project, two FLRD set-ups at 1535 nm and 808 nm were designed. In both set-ups, optical fiber and optical fiber couplers are used to form a cavity. At 1535 nm, a FLRD set-up is constructed by utilizing a pulsed laser and used for characterization of thin films, various pure liquids and fluorescein solutions. Two different sensor regions are designed with free space collimators and ferrules for the measurement of thin films and liquids, respectively. The future endeavor of the set-up is improvement for reliability and reproducibility of the system. For visible and NIR regions, a fiber coupled laser with four colors as 642 nm, 785 nm, 808 nm and 852 nm laser is used to design of a FLRD set-up. 808 nm laser is selected to build a prototype of the FLRD system. The construction of a closed loop FLRD set-up is completed and the system is characterized. Ultimate aim in our project is to be able to do trace detection at visible and NIR regions where the chemical sensitivity is higher.

The use of geophysical testing methods has considerable potential to be a cost effective and accurate technique to assess near-surface soil conditions. Multi channel analysis of surface waves (MASW) test is a geophysical non-intrusive test that uses the dispersive characteristic of Rayleigh waves to estimate low strain shear modulus and damping coefficient of near-surface soil. Also, this technique is used to detect underground voids. Recently, MASW technique has gained more attention, partly because of its ease of use and partly because of the significant improvements in data acquisition systems. The theories of MASW test consider the effect of horizontal soil layering, though the effect of lateral inhomogeneities (i. e. cavities and voids), inclined layering and inverse layering (i. e. a layered system in which the top layers are stiffer than the bottom ones) are not addressed properly in these theories. <br /><br /> The objective of this dissertation is to investigate the effect of lateral inhomogeneities on the propagation of Rayleigh waves in an elastic half-space excited by a transient loading. The results can be applied to locate underground cavities using MASW test and to improve the MASW analysis techniques. In lieu of theoretical solutions, two and three dimensional numerical models are constructed to simulate the MASW test. To assure the quality of the obtained data, numerical models are calibrated with Lamb solution. Voids with different sizes and embedment depths are inserted in the medium. Responses along the surface as well as inside the medium are recorded and analyzed in time, frequency, spatial and frequency-wave number domains. Different material types and sources are used to generalize the results. Afterwards, the combined effect of void and layered systems on the surface responses are studied. To verify the results, experimental field and laboratory data are presented and the trends are compared to the numerical results. <br /><br /> It is found that the void starts to vibrate in response to the Rayleigh wave excitation. Due to the vibration of the void energy partitioning occurs. Part of the incident energy is reflected in the form of Rayleigh wave. Another part is converted to body waves, and spread into the medium. The transferred part of the energy is attenuated and has smaller amplitudes. Finally, a part of energy is trapped in the void region and bounces back and forth between the void boundaries, until it damps. The trapped energy is associated to higher modes of Rayleigh waves and excited Lamb waves. The effect of trapped energy is seen as a region in the vicinity of the void with concentrated energy, in frequency domain. The extents of this region depends on the void size, and the frequency content of the incident energy. Thus, in some cases it is possible to correspond the size of the model to the extents of the region with energy concentration. <br /><br /> A new technique is proposed to determine the location of a void, and estimate its embedment depth. The technique is called Attenuation Analysis of Rayleigh Waves (AARW), and is based on the observed damping effect of the void on the surface responses. For verification, the results are compared to experimental field and laboratory data. The observations are in good agreement with the observed numerical results. Further, the AARW technique showed to be a promising tool for void detection.

Time integration methods are necessary for the solution of transient flow problems. In recent years, interest in transient flow problems has increased, leading to a need for better understanding of the costs and benefits of various time integration schemes. The present work investigates two common time integration schemes, namely the Semi-Implicit Method for Pressure-Linked Equations (SIMPLE) and the Fractional Step (FS) method. Three two-dimensional, transient, incompressible flow problems are solved using a cell centered, finite volume code. The three test cases are laminar flow in a lid-driven skewed cavity, laminar flow over a square cylinder, and turbulent flow over a square cylinder. Turbulence is modeled using wall functions and the k - ε turbulence model with the modifications suggested by Kato and Launder. Solution efficiency as measured by the effort carried out by the flow equation solver and CPU time is examined. Accuracy of the results, generated using the SIMPLE and FS time integration schemes, is analyzed through a comparison of the results with existing experimental and/or numerical solutions. Both the SIMPLE and FS algorithms are shown to be capable of solving benchmark flow problems with reasonable accuracy. The two schemes differ slightly in their prediction of flow evolution over time, especially when simulating very slowly changing flows. As the time step size decreases, the SIMPLE algorithm computational cost (CPU time) per time step remains approximately constant, while the FS method experiences a reduction in cost per time step. Also, the SIMPLE algorithm is numerically stable for time steps approaching infinity, while the FS scheme suffers from numerical instability if the time step size is too large. As a result, the SIMPLE algorithm is recommended to be used for transient simulations with large time steps or steady state problems while the FS scheme is better suited for small time step solutions, although both time-stepping schemes are found to be most efficient when their time steps are at their maximum stable value.

Terahertz waveguides are the focus of considerable research interest due to their potential for sensing, imaging and communications applications. Two of the most promising designs are the metal wire waveguide and the parallel-plate waveguide. The metal wire waveguide exhibits excellent low loss and low dispersion characteristics. However, the radiation is only weakly coupled to the wire and the beam extends a great distance from the waveguide, which can lead to high bending loss. In my research I show that this large beam extent also gives a high degree of flexibility in the geometry required to couple radiation into the waveguide or between waveguide sections. I also show that the traditional formalism of bending loss is incomplete, and that there is an optimum radius of curvature to reduce loss. The relationship between the beam extent and the radius of the wire presents the possibility of a tapered waveguide to confine the radiation as it propagates. I here present experimental data and simulations results to verify this subwavelength confinement at the tip of a tapered metal wire waveguide, which is of great interest for near-field imaging applications. The parallel-plate waveguide is another design frequently employed due to its low loss and low dispersion characteristics. Resonant structures may also be easily incorporated into the waveguide for sensing and filtering applications. One such structure is a single rectangular groove, which serves as a notch filter with a very narrow linewidth when the transverse-electric (TE) mode of the waveguide is excited, though its physical origin is poorly understood. In this work I present a detailed experimental and theoretical study of the rectangular resonant cavity in a TE-mode parallel-plate waveguide, particularly with respect to its potential as a microfluidic refractive index sensor. This study is extended to include the possibility of two grooves, in both coupled and non-coupled geometries, and their efficacy as multichannel or high-resolution single-channel microfluidic sensors.

The use of geophysical testing methods has considerable potential to be a cost effective and accurate technique to assess near-surface soil conditions. Multi channel analysis of surface waves (MASW) test is a geophysical non-intrusive test that uses the dispersive characteristic of Rayleigh waves to estimate low strain shear modulus and damping coefficient of near-surface soil. Also, this technique is used to detect underground voids. Recently, MASW technique has gained more attention, partly because of its ease of use and partly because of the significant improvements in data acquisition systems. The theories of MASW test consider the effect of horizontal soil layering, though the effect of lateral inhomogeneities (i. e. cavities and voids), inclined layering and inverse layering (i. e. a layered system in which the top layers are stiffer than the bottom ones) are not addressed properly in these theories. <br /><br /> The objective of this dissertation is to investigate the effect of lateral inhomogeneities on the propagation of Rayleigh waves in an elastic half-space excited by a transient loading. The results can be applied to locate underground cavities using MASW test and to improve the MASW analysis techniques. In lieu of theoretical solutions, two and three dimensional numerical models are constructed to simulate the MASW test. To assure the quality of the obtained data, numerical models are calibrated with Lamb solution. Voids with different sizes and embedment depths are inserted in the medium. Responses along the surface as well as inside the medium are recorded and analyzed in time, frequency, spatial and frequency-wave number domains. Different material types and sources are used to generalize the results. Afterwards, the combined effect of void and layered systems on the surface responses are studied. To verify the results, experimental field and laboratory data are presented and the trends are compared to the numerical results. <br /><br /> It is found that the void starts to vibrate in response to the Rayleigh wave excitation. Due to the vibration of the void energy partitioning occurs. Part of the incident energy is reflected in the form of Rayleigh wave. Another part is converted to body waves, and spread into the medium. The transferred part of the energy is attenuated and has smaller amplitudes. Finally, a part of energy is trapped in the void region and bounces back and forth between the void boundaries, until it damps. The trapped energy is associated to higher modes of Rayleigh waves and excited Lamb waves. The effect of trapped energy is seen as a region in the vicinity of the void with concentrated energy, in frequency domain. The extents of this region depends on the void size, and the frequency content of the incident energy. Thus, in some cases it is possible to correspond the size of the model to the extents of the region with energy concentration. <br /><br /> A new technique is proposed to determine the location of a void, and estimate its embedment depth. The technique is called Attenuation Analysis of Rayleigh Waves (AARW), and is based on the observed damping effect of the void on the surface responses. For verification, the results are compared to experimental field and laboratory data. The observations are in good agreement with the observed numerical results. Further, the AARW technique showed to be a promising tool for void detection.

Time integration methods are necessary for the solution of transient flow problems. In recent years, interest in transient flow problems has increased, leading to a need for better understanding of the costs and benefits of various time integration schemes. The present work investigates two common time integration schemes, namely the Semi-Implicit Method for Pressure-Linked Equations (SIMPLE) and the Fractional Step (FS) method. Three two-dimensional, transient, incompressible flow problems are solved using a cell centered, finite volume code. The three test cases are laminar flow in a lid-driven skewed cavity, laminar flow over a square cylinder, and turbulent flow over a square cylinder. Turbulence is modeled using wall functions and the k - ε turbulence model with the modifications suggested by Kato and Launder. Solution efficiency as measured by the effort carried out by the flow equation solver and CPU time is examined. Accuracy of the results, generated using the SIMPLE and FS time integration schemes, is analyzed through a comparison of the results with existing experimental and/or numerical solutions. Both the SIMPLE and FS algorithms are shown to be capable of solving benchmark flow problems with reasonable accuracy. The two schemes differ slightly in their prediction of flow evolution over time, especially when simulating very slowly changing flows. As the time step size decreases, the SIMPLE algorithm computational cost (CPU time) per time step remains approximately constant, while the FS method experiences a reduction in cost per time step. Also, the SIMPLE algorithm is numerically stable for time steps approaching infinity, while the FS scheme suffers from numerical instability if the time step size is too large. As a result, the SIMPLE algorithm is recommended to be used for transient simulations with large time steps or steady state problems while the FS scheme is better suited for small time step solutions, although both time-stepping schemes are found to be most efficient when their time steps are at their maximum stable value.