Global ETD Search

1	Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs / DMD患者由来iPS細胞におけるマルチエクソンスキッピング誘導に向けたDual CRISPR-Cas3システム Kita, Yuto 23 January 2024 (has links) 付記する学位プログラム名: 京都大学卓越大学院プログラム「メディカルイノベーション大学院プログラム」 / 京都大学 / 新制・課程博士 / 博士(医科学) / 甲第25007号 / 医科博第154号 / 新制\|\|医科\|\|10(附属図書館) / 京都大学大学院医学研究科医科学専攻 / (主査)教授遊佐宏介, 教授萩原正敏, 教授齋藤潤 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM genetic disorder Duchenne muscular dystrophy genome editing CRISPR-Cas system CRISPR-Cas3 491
2	The CRISPR-Cas system Stens, Cassandra, Enoksson, Isabella, Berggren, Sara January 2020 (has links) Derived from and inspired by the adaptive immune system of bacteria, CRISPR has gone from basic biology knowledge to a revolutionizing biotechnological tool, applicable in many research areas such as medicine, industry and agriculture. The full mechanism of CRISPR-Cas9 was first published in 2012 and various CRISPR-Cas systems have already passed the first stages of clinical trials as new gene therapies. The immense research has resulted in continuously growing knowledge of CRISPR systems and the technique seems to have the potential to greatly impact all life on our planet. Therefore, this literature study aims to thoroughly describe the CRISPR-Cas system, and further suggest an undergraduate laboratory exercise involving gene editing with the CRISPR-Cas9 tool. In this paper, we describe the fundamental technical background of the CRISPR-Cas system, especially emphasizing the most studied CRISPR-Cas9 system, its development and applications areas, as well as highlighting its current limitations and ethical concerns. The history of genetic engineering and the discovery of the CRISPR system is also described, along with a comparison with other established gene editing techniques. This study concludes that a deeper knowledge about CRISPR is important and required since the technique is applicable in many research areas. A laboratory exercise will not only inspire but also provide extended theoretical and practical knowledge for undergraduate students. CRISPR CRISPR-Cas system CRISPR-Cas9 genetic engineering genome editing gene editing biotechnology CRISPR classification eukaryotic cells HDR NHEJ double stranded break CRISPR locus spacer acquisition CRISPR delivery RNP dCas9 nCas9 prime editing base editing CRISPRa CRISPRi CRISPR history multiplexing diagnostics gene drive anti-CRISPR designer babies CRISPR ethics. Natural Sciences Naturvetenskap Biological Sciences Biologiska vetenskaper Biochemistry and Molecular Biology Biokemi och molekylärbiologi Genetics Genetik

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Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs / DMD患者由来iPS細胞におけるマルチエクソンスキッピング誘導に向けたDual CRISPR-Cas3システム

The CRISPR-Cas system