Orientador: Frederico Ozanam Papa / Banca: João Carlos Pinheiro Ferreira / Banca: Alício Martins Junior / Resumo: A despeito das inúmeras variáveis que influenciam direta e indiretamente a fertilidade das fêmeas bovinas, a qualidade das amostras seminais exerce um papel importante na determinação das taxas de concepção dos programas de inseminação artificial. Os objetivos dessa pesquisa foram comparar a efetividade de dois diluidores de criopreservação de sêmen bovino no processamento de amostras seminais apresentando diferentes concentrações espermáticas em relação aos índices de congelabilidade determinados laboratorialmente (Experimento I) e as taxas de concepção proporcionadas por cada metodologia quando utilizada em programas de inseminação artificial em tempo fixo (IATF) em bovinos (Experimento II). No Experimento I foram utilizados 14 ejaculados de diferentes touros da raça Nelore. Cada ejaculado foi fracionado em oito alíquotas iguais, submetidas a criopreservação com os diluidores Tris-gema de ovo-frutose (meio TRIS) e MKA nas concentrações de 12, 25, 50 e 100 milhões de espermatozóides totais por mililitro de meio, formando oito grupos experimentais em função das variáveis diluidor e concentração. As amostras foram descongeladas a 46 ºC por 20 segundos, avaliando-se os padrões de motilidade através do método computadorizado (CASA), integridade de membrana plasmática (IMP), resistência ao teste de termorresistência rápido (TTR) e taxa de recuperação e IMP após seleção espermática pela técnica de swim-up. Para o Experimento II foram selecionados sete touros utilizados no Experimento I, obtendo-se um ejaculado de cada animal por eletroejaculação...(Resumo completo, clicar acesso eletrõnico abaixo) / Abstract: Although there are many variables which directly or indirectly influence female bovine fertility, the quality of sperm samples plays a important role in the determination of conception rates in artificial insemination programs. The aim of the present study was to compare the efficiency of two bovine semen extenders for sperm freezing with different spermatic concentrations in the freezability determined by lab tests (Experiment I), and conception rates after fixed time artificial insemination (FTAI; Experiment II). In Experiment I 14 ejaculates of different Nelore bulls were used. Each ejaculate was splitsampled in to eight equal parts and then submitted to cryopreservation with Tris-egg yolk fructose (TRIS) and MKA extenders, at concentrations of 12, 25, 50 and 100 millions spermatozoa per milliliter forming eight experimental groups. The samples were thawed at 46 ºC for 20 seconds, and the following parameters were evaluated: sperm motility and movement (by computer-assisted semen analysis - CASA), sperm membrane integrity (SMI), resistance to the fast thermoresistance test (TT), recovery rate and sperm membrane integrity after sperm selection through swim-up technique. Seven of 14 bulls used in Experiment I were selected for Experiment II, and semen was collected from each of the animals by electroejaculation. The seven ejaculates obtained were mixed (semen pool) and cryopreserved, thus forming eight experimental groups according to the freezing extenders and sperm concentrations/straws: TRIS 12, 25, 50 and 100, and MKA 12, 25, 50 and 100...(Complete abstract, click electronic address below) / Mestre

The objective of the study was to evaluate cow reproductive performance in the sweetveld and sourveld communal grazing areas of the Eastern Cape Province, South Africa. In the first experiment, farmer perceptions were obtained using participatory rural appraisals. Farmers ranked lack of fences, tick-borne diseases, poor animal condition during winter and poor breeding practices, respectively as major constraints limiting cattle production in the Eastern Cape. Cattle, sheep and goats, in that order, were ranked as the most important livestock species and were mainly kept for meat, cash and ceremonies, respectively. The non-descript cattle breed was the most common breed found in the smallholder areas. Most farmers preferred Nguni breed because of its adaptive attributes. In the second experiment, structured questionnaires were administered, between June and August 2006, to a total of 551 farmers from 10 communities of the Eastern Cape. There was a significant association (P<0.05) between the use of pregnancy diagnoses and community. About 87 and 77 % of the interviewed farmers did not respond on the extent of pregnancy and calving rates in their herds. A higher proportion of farmers from Hekele (51%) and from Upper Mnxe (45.3%) communities reported low number of bulls as a major constraint to cow reproductive performance. Body condition and ovarian activity were measured in the sweet and sour veld types. Body condition score of animals was measured from March iii 2007 until January 2008 and ovarian activity of cows was performed by a veterinarian through rectal palpation in June, August and October 2007 and January 2008. From March to July, there was a marked decline in body condition on both veld types. In the sweetveld, body condition improved from September until January, whereas in the sourveld the improvement in body condition started in October. The cows in both veld types conceived throughout the year. Most cows in the sweetveld were cycling in January and August (P<0.05) whereas in the sourveld there was no distinct period when the animals were cycling. Overall, there were no differences in the proportion of cows that were cycling between the sour and sweet veldts (P>0.05). There were more cows cycling in sourveld in October than in the sweetveld. Reproductive performance of cows in communal areas could, therefore, be determined by levels and quality of nutrition. Keywords: Participatory rural appraisals; Structured questionnaires; Farmer participation; Farmer perceptions; Body condition scoring; Ovarian activity; Pregnancy diagnoses.

Institute for Rural Development / MRDV

Made available in DSpace on 2014-06-11T19:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-26Bitstream added on 2014-06-13T19:38:31Z : No. of bitstreams: 1 crespilho_am_me_botfmvz.pdf: 507525 bytes, checksum: a1c92f545e77b50838125fbea8dfddd6 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A despeito das inúmeras variáveis que influenciam direta e indiretamente a fertilidade das fêmeas bovinas, a qualidade das amostras seminais exerce um papel importante na determinação das taxas de concepção dos programas de inseminação artificial. Os objetivos dessa pesquisa foram comparar a efetividade de dois diluidores de criopreservação de sêmen bovino no processamento de amostras seminais apresentando diferentes concentrações espermáticas em relação aos índices de congelabilidade determinados laboratorialmente (Experimento I) e as taxas de concepção proporcionadas por cada metodologia quando utilizada em programas de inseminação artificial em tempo fixo (IATF) em bovinos (Experimento II). No Experimento I foram utilizados 14 ejaculados de diferentes touros da raça Nelore. Cada ejaculado foi fracionado em oito alíquotas iguais, submetidas a criopreservação com os diluidores Tris-gema de ovo-frutose (meio TRIS) e MKA nas concentrações de 12, 25, 50 e 100 milhões de espermatozóides totais por mililitro de meio, formando oito grupos experimentais em função das variáveis diluidor e concentração. As amostras foram descongeladas a 46 ºC por 20 segundos, avaliando-se os padrões de motilidade através do método computadorizado (CASA), integridade de membrana plasmática (IMP), resistência ao teste de termorresistência rápido (TTR) e taxa de recuperação e IMP após seleção espermática pela técnica de swim-up. Para o Experimento II foram selecionados sete touros utilizados no Experimento I, obtendo-se um ejaculado de cada animal por eletroejaculação... / Although there are many variables which directly or indirectly influence female bovine fertility, the quality of sperm samples plays a important role in the determination of conception rates in artificial insemination programs. The aim of the present study was to compare the efficiency of two bovine semen extenders for sperm freezing with different spermatic concentrations in the freezability determined by lab tests (Experiment I), and conception rates after fixed time artificial insemination (FTAI; Experiment II). In Experiment I 14 ejaculates of different Nelore bulls were used. Each ejaculate was splitsampled in to eight equal parts and then submitted to cryopreservation with Tris-egg yolk fructose (TRIS) and MKA extenders, at concentrations of 12, 25, 50 and 100 millions spermatozoa per milliliter forming eight experimental groups. The samples were thawed at 46 ºC for 20 seconds, and the following parameters were evaluated: sperm motility and movement (by computer-assisted semen analysis - CASA), sperm membrane integrity (SMI), resistance to the fast thermoresistance test (TT), recovery rate and sperm membrane integrity after sperm selection through swim-up technique. Seven of 14 bulls used in Experiment I were selected for Experiment II, and semen was collected from each of the animals by electroejaculation. The seven ejaculates obtained were mixed (semen pool) and cryopreserved, thus forming eight experimental groups according to the freezing extenders and sperm concentrations/straws: TRIS 12, 25, 50 and 100, and MKA 12, 25, 50 and 100...(Complete abstract, click electronic address below)

Thesis (M.A. Agricultural Management (Animal Production)) -- University of Limpopo, 2020 / The Nguni is indigenous multi-purpose cattle breed that plays an important role in both commercial and communal farming systems in South Africa (SA). Unfortunately, the breed is currently under threat of diminishing due to farmers’ preference for exotic breeds and cross breeding. In recognition of the above, the Industrial Development Corporation, the Mpumalanga Department of Agriculture, Rural Development, Land and Environmental Affairs and the University of Limpopo established the Nguni cattle breed preservation project. In this project, a herd of 30 Nguni heifers and 1 bull were allocated to 34 Communal Property Associations (CPA) and 40 private farming enterprises each. With its three distinct ecological zones (High, Mid and Low-veld), the Mpumalanga province poses a challenge to the Nguni preservation project since cattle have to adapt to different ecological zones in order to grow and reproduce efficiently. Knowledge of cattle herd productivity improves the understanding of the functional attributes and is useful in determining the constraints and the potential of communal beef cattle production systems. Significant differences between ecological zones were observed for age at first calving, weaning rate, calves’ birth weight and calves’ weaning weight (p < 0.05). Calving of heifers in the Mid-veld zone occurred earlier (24 months) on both ownership types compared to Highveld (28.09 months for private and 26.00 for CPA) and the Lowveld (28.35 months for both ownerships). Nguni cattle on the Highveld performed better in terms of weaning rate under both private and CPA ownerships, where the weaning rate were 93% and 80%, respectively. Calves birth weight was lower in both ownership types in the Midveld zone (22.17 kg), whereas in the CPA, the Lowveld zone had higher calves birth weights (26.80 kg). In the private ownership, the highest calves’ birth weight (25.35 kg) was on the Highveld. The calves weaning weight were significantly higher (p < 0.05) on Highveld in private ownership (190.16 kg) and lowest (160.39 kg) in the Midveld zone. For the CPA, the highest calves weaning weight was also on the Highveld (187.55) and lowest on Midveld (167.50 kg). A full range of backup services that are offered to the communities by stakeholders in the form of a beef package that includes veld and pasture management, nutrition management, beef performance, animal recording keeping, genetic evaluation and animal health management program needs thorough attention.