In this dissertation, channel modeling for Terahertz (THz) channels and designs of nano devices for THz communications are studied. THz communication becomes more and more important for future wireless communication systems that require an ultra high data rate, which motivates us to propose new nano device designs based on graphene and new system models for the THz channel. Besides, the multiple-input multiple-output (MIMO) antenna technique is well known to increase the spectral efficiency of a wireless communications system. Considering THz channels' particular characteristics, MIMO systems with reconfigurable antennas and distributed antennas are proposed. We compare the differences between MIMO systems in the GHz and THz bands, and highlight the benefits of using multi antennas in the THz band. The work on nano device designs provides two antenna designs with single walled carbon nanotubes (SWCNTs) and graphene nano ribbon (GNR). First, we analyse the spectral efficiency of an SWCNT bundled dipole antenna based MIMO system in the Terahertz band. Two scenarios are considered: the large scale MIMO and the conventional scale MIMO. It is found that, in order to get the maximum spectral efficiency, the CNT bundle size should be optimized to obtain a tradeoff between the antenna efficiency and the number of antennas for a given area. We also discuss the random fluctuation in the bundle size during the CNT bundled antenna fabrication which reduces the system spectral efficiency. Then, we propose reconfigurable directional antennas for THz communications. The beamwidth and direction can be controlled by the states of each graphene patch in the antenna, and the states can be easily configured by changing the electrostatic bias voltage on each element. The work on reconfigurable MIMO system proposes a new antenna array design for MIMO in the THz band. First, the path loss and reflection models of the THz channel are discussed. Then, we combine the graphene-based antenna and the THz channel model and propose a new MIMO antenna design. The radiation directions of the transmit antennas can be programmed dynamically, leading to different channel state matrices. Finally, the path loss and the channel capacity are numerically calculated and compared with those of the GHz channel. The results show that for short range communications, the proposed MIMO antenna design can enlarge the channel capacity by both increasing the number of antennas and choosing the best channel state matrices. The work on MIMO channels proposes a statistical model for the MIMO channel with rough reflection surfaces in the THz Band. First, our analysis of scattering from a rough surface indicates that the reflection from a single surface can be a cluster of rays. Secondly, a new MIMO model for THz communications is proposed. In this model, the number of multipaths is highly dependent on the roughness of the reflecting surfaces. When the surface is ideally smooth, the MIMO channel is sparse and as a result, the capacity is sub-linear with the MIMO scale. On the other hand, when the surface is rough, more degrees of freedom are provided by the scattered rays. Finally, channel capacities with different surface roughness are numerically calculated and compared between different MIMO scales. The results show that in contrast to the GHz range, large scale THz multiple antennas may not provide as much multiplexing gain. Therefore, it is necessary to determine the antenna scale according to the actual propagation environment. The work on distributed antenna systems (DAS) proposes a new DAS model in the THz band. First, the model of DAS in the THz frequency is discussed, which has fewer multipaths than that in the GHz band. Then, we analyze the characteristics of the DAS model and point out that the channel is very sparse if the number of antennas on the base station (BS) is very large. Besides, we provide reasons for the fact that DAS can have a large number of degrees of freedom. We compare the capacities of MIMO systems with DAS and without DAS. The results show that for THz channels, increasing the number of antenna units (AUs) is much more important than increasing the number of antennas in one AU. Finally, we propose an antenna selection and precoding scheme which has very low complexity. / Graduate

This study was undertaken in order to provide further insight into the effects of artificial heating on the fisheries of a small reservoir in the Southwest. The following specific objectives were established: (1) to map the reservoir for the distribution of heated water, (2) to determine the distribution of selected species of sports, rough, and forage fish in areas affected by the effluent and in areas not affected, (3) to compare size and condition of selected species of fish from areas affected by the effluent to size and condition of fish from areas not affected, and (4) to compare food habits of channel catfish in areas affected by the effluent to the food habits of channel catfish in areas not affected.

The requirements for future DVB-T/H networks demand that broadcasters design and deploy networks that provide ubiquitous reception in challenging indoors and other obstructed situations. It is essential that such networks are designed cost-effectively and with minimized environmental impact. The use of transmit diversity techniques with multiple antennas have long been proposed to improve the performance and capacity of wireless systems. Transmit diversity exploits the scattering effect inherent in the channel by means of transmitting multiple signals in a controlled manner from spatially separated antennas, allowing independently faded signals to arrive at the receiver and improves the chances of decoding a signal of acceptable quality. Transmit diversity can complement receive diversity by adding an additional diversity gain and in situations where receiver diversity is not practical, transmit diversity alone delivers a comparable amount of diversity gain. Transmit Delay Diversity (DD) can be applied to systems employing the DVB standard without receiver equipment modifications. Although transmit DD can provide a gain in NLOS situations, it can introduce degradation in LOS situation. The aim of this thesis is to investigate the effectiveness in real-word applications of novel diversity techniques for broadcast transmitter networks. Tests involved laboratory experiments using a wireless MIMO channel emulator and the deployment of a field measurement campaign dedicated to driving, indoor and rooftop reception. The relationship between the diversity gain, the propagation environment and several parameters such as the transmit antenna separation, the receiver speed and the Forward Error Correction Codes (FEC) configuration are investigated. Results includes the effect of real-word parameter usually not modeled in the software simulation analysis, such as antenna radiation patterns and mutual coupling, scattering vegetation impact, non-Gaussian noise sources and receiver implementation. Moreover, a practical analysis of the effectiveness of experimental techniques to mitigate the loss due to transmit DD loss in rooftop reception is presented. The results of this thesis confirmed, completed and extended the existing predictions with real word measurement results.

Phosphatidylinositol bisphosphate (PIP2) directly regulates electrophysiological activity in a diverse family of ion channels whether the effect is stimulatory or inhibitory. Much has been unveiled about the apparent affinity and modulatory function of PIP2 using a chemically modified dioctanoyl PIP2 (diC8), a membrane delimited cytosolic co-factor in inside-out macropatch experiments. Yet, the scarcity of molecular tools that permit fine external control in whole-cell systems has precluded future studies from probing the physiological role of PIP2 in cells in the presence of a fully intact cytoplasm. Here we introduce light as an external control for PIP2 through photocaging of diC8, and test its activation of Kir2.3 (IRK3), an inwardly rectifying ion channel that has previously shown to possess moderate binding affinity to PIP2, in excised, inside-out macropatches. Our experiments revealed that photocaged-diC8 and irradiated photocaged-diC8 have significantly different activation kinetics than the fully active diC8. Surprisingly, the activation of caged-diC8 by UV irradiation attenuated Kir2.3 activity, while the inactivated diC8 (caged-diC8) resulted in similar magnitude of channel activity compared to the currents elicited by unmodified diC8. Interestingly, we also show that application of both activated (irradiated) and inactive (caged) diC8 in macropatches generated highly fluctuating ion channel activity.

The emergence of pyrethroid resistant Anopheles funestus (a major African vector) in malaria affected parts of KwaZuluNatal, South Africa was correlated with the malaria epidemic of 1996 2000. This finding prompted the necessity of incorporating insecticide resistance management strategies into formal malaria control policy in South Africa. Resistance management strategies often rely on the assumption of reduced fitness associated with insecticide resistance and are based on the principle that resistance genes will tend to drift out of vector populations in the absence of insecticide selection pressure. This study aimed to determine whether a fitness cost is associated with pyrethroid resistance as well as to determine the stability and mode of inheritance of the resistance genes in a pyrethroid resistant (FUMOZR) strain of An. funestus. It also aimed to sequence and analyze a segment of the sodium channel gene for any kdrtype mutation(s) that may be associated with pyrethroid resistance. The final aim was to determine the resistance mechanisms involved in a Ghanaian field population of An. funestus resistant to DDT and pyrethroids. Results obtained suggest that pyrethroid resistance in southern African An. funestus did not incur any loss of fitness. FUMOZR had a reproductive advantage over a pyrethroid susceptible An. funestus strain (FANG) in terms of higher fertility, proportion of females laying eggs and eggtoadult survivorship, and a lower sterility Click to buy NOW! PDFXCHANGE www.docutrack. com Click to buy NOW! PDFXCHANGE www.docutrack. com iv rate. However, FUMOZR had a slower developmental time from egg hatch to adult emergence than FANG. Results of crosses and backcrosses carried out between FUMOZR and FANG were consistent with a monofactorial and autosomal mode of inheritance in which the resistant genes presented as incompletely dominant. The resistant gene was found to be stable over several generations in the absence of insecticide selection pressure. Analysis of the genomic and mRNA sequences of the IIS5 IIS6 segment of the sodium channel gene showed a high sequence identity between FUMOZR and FANG suggesting that the two strains are genetically similar. The kdrtype mutation was absent from this region supporting previous evidence that the resistance mechanism is primarily metabolic. Bioassay data showed that a Ghanaian field population of An. funestus from Obuasi, Ghana, was resistant to DDT and pyrethroids. Molecular analysis of the IIS5 IIS6 segment of the sodium channel gene showed an absence of kdrtype mutations previously associated with insecticide resistance. Biochemical analysis suggests that resistance is metabolically mediated primarily by elevated levels of and esterases with monooxygenases and GSTs playing a lesser role. The presence of an altered acetylcholinesterase conferring carbamate resistance was also evident in the population. These results have implications for the management of resistance in malaria control programmes in Africa.

ABSTRACT The Chloride Intracellular Channel (CLIC) proteins are a family of amphitropic proteins that can convert from soluble to integral membrane forms. CLIC1 is a member of this family that functions as a chloride channel in the plasma and nuclear membranes of cells. Although high-resolution structural data exists for the soluble form of monomeric CLIC1, not much is known about the integral membrane forms’ structure. The exact mechanism and signals involved in the conversion of the soluble form to membrane-inserted form are also not clear. Studies were undertaken in the absence and presence of membrane models. Analysis of the structure and stability of CLIC1 in the absence of membrane investigated the effect of possible signals or triggers that may play a crucial role in the conversion of the soluble form to integral membrane form. Exposing CLIC1 to oxidizing conditions results in the formation of a dimeric form. The CLIC1 dimer was found to be less stable than the monomeric form based on unfolding kinetic studies. The stability of the dimer was also less influenced by salt concentration, compared with the monomer. The effect of pH on the structure of CLIC1 is of physiological relevance since the movement of soluble CLIC1 in the cytoplasm or nucleoplasm toward the membrane will involve the protein being exposed to a lower pH micro-environment. Hydrogen exchange mass spectrometry was used to study the structural dynamics of CLIC1 at pH 7.0 and pH 5.5. At neutral pH, domain II is more stable than the more flexible thioredoxin domain I. The thioredoxin-fold therefore is more likely to unfold and rearrange to insert into membranes. Because of the high stability of domain II this region is probably where the folding nucleus of the protein is. At pH 5.5 it was found that the a1, a3 and a6 helices, which are spatially adjacent to one another across the domain interface, were destabilized. This destabilization may be the trigger for CLIC1 to unfold and rearrange into a membrane insertion-competent form. The role of the primary sequence and unique three-dimensional structure of CLIC1 in membrane insertion was investigated in a bioinformatics-based study that looked at conserved residue features such as hydropathy and charge. Hidden helical propensities and Ncapping motifs in the a1-b2 region were found, which may have important implications for locating putative transmembrane regions. Analysis of the structure and thermodynamics of CLIC1 interacting with membranes investigated changes in secondary structure, tertiary structure, hydrodynamic volume and thermodynamics when CLIC1 is exposed to membrane-mimicking models. The effect of a variety of conditions such as pH and redox, cysteine-modifiying agents (NEM), ligands (GSH), and inhibitors (IAA) on CLIC1 membrane interaction were studied. It was found that CLIC1 interacted with membranes more favourably at lower pH and that NEM completely inhibited CLIC1 interaction with micelles.

Ankyrin receptor TRPA1 is an ion channel widely expressed on primary afferent sensory neurons, where it acts as a polymodal sensor of nociceptive stimuli. Apart from pungent chemicals (e. g. isothiocyanates, cinnamaldehyde and its derivatives, acrolein, menthol), it could be activated by cold temperatures, depolarizing voltages or intracellular calcium ions. TRPA1 channel is a homotetramer in which each subunit consists of cytoplasmic N and C termini and a transmembrane region. The transmembrane part is organized into six alpha- helices connected by intra- and extracellular loops. The N terminus comprises a tandem set of 16 to 17 ankyrin repeats (AR), while the C terminus has a substantially shorter, dominantly helical structure. In 2015, a partial cryo-EM structure of TRPA1 was resolved; however, the functional roles of the individual regions of the receptor have not yet been fully understood. This doctoral thesis is concerned to elucidate the role of highly conserved sequence and structural motifs within the cytoplasmic termini and the S4-S5 region of TRPA1 in voltage- and chemical sensitivity of the receptor. The probable binding site for calcium ions that are the most important physiological modulators of TRPA1 was described by using homology modeling, molecular-dynamics simulations,...

Este trabalho apresenta um modelo matemático de suporte à decisão para projeto de canais com seção trapezoidal, cujo regime de escoamento seja do tipo unidimensional, fluvial e permanente, para terrenos naturais. A seção é otimizada pelo critério de custo global, composto pelos custos de implantação (motorização, escavação, aterro, revestimento) e manutenção (conservação e operação, contabilizada através da perda de energia). A determinação da seção ótima foi feita por algoritmo de programação não linear. As variáveis analisadas foram o revestimento (custo, espessura, coeficiente de Manning), a vazão, a declividade de fundo do canal, a vida útil, taxa de juros, seções topográficas (com horizontes geotécnicos), e o resultado contempla a largura de fundo e consequentemente a altura da seção. / This work presents a decision support mathematical model for design of trapezoidal sections channel, for the case of one-dimensional, subcritical, steady and open-channel flow. The section is optimized by the criterion of total cost, composed by the costs of implementation (engine, excavation, landfill, lining) and maintenance (conservation and operation, accounted for by the loss of energy). The determination of optimum section was performed by nonlinear programming algorithm. The variables analyzed were the lining (cost, thickness, Mannings coefficient), flow, the channel bottoms slope, the project lifetime, interest rates, topographic sections (including geotechnical data), and the result includes the width and consequently the height of the section.

S-palmitoylation is a reversible post-translational lipid modification of proteins by adding a 16-carbon palmitate onto a cysteine residue. Palmitoylation has been shown to control the trafficking and function of many signalling proteins including ion channels. In this Thesis, palmitoylation is shown to control both the plasma membrane expression and gating properties of large conductance calcium- and voltage- dependent potassium (BK) channels. The BK channel is assembled from four pore-forming α-subunits. Each α-subunit contains seven transmembrane domains (S0-S6), with an extracellular N-terminus and a large intracellular C-terminus. BK channel α-subunit is encoded by a single gene Kcnma1 that undergoes extensive pre mRNA splicing at various splice sites, thus there are a number of alternatively spliced variants of α-subunits. Using quantitative imaging assays, palmitoylation of the intracellular S0-S1 loop controlled trafficking of full length ZERO variant BK channels to the plasma membrane in HEK293 cells as well as neuronal N2a cells. Importantly, all four α-subunits need to be palmitoylated for robust surface expression. Thus, palmitoylation of the S0-S1 loop of the α-subunit is important for surface expression of BK channels. The BK channel may also assemble with auxiliary β-subunits (β1-4) that regulate surface expression and gating properties of BK channels. The N-terminus of the β1- subunit and the C-terminus of the β4-subunit were shown to be palmitoylated using [3H]-palmitate incorporation, respectively. However, mutation of the palmitoylated cysteine (C18 in β1 and C193 in β4) to alanine to generate depalmitoylated β- subunits had no significant effects on the electrophysiological properties resulting from co-expression with the ZERO variant of the BK channel. However, although palmitoylation of the S0-S1 loop does not affect the electrophysiological properties of the ZERO channels alone, it is important for the shift in the V0.5max of ZERO channel when co-expressed with the β1-subunit, but not β4-subunit. These data suggest that palmitoylation of the S0-S1 loop controls the functional coupling between the ZERO α-subunit and β1-subunit. Although palmitoylation of C18 in the N-terminus of the β1-subunit was not required for functional coupling to α-subunits, we identified other critical residues within the short intracellular N-terminus of the β1-subunit that are essential. The functional coupling between BK α- and β1-subunit was predicted to be controlled by the interaction between a non-classic amphipathic α-helix in the β1 subunit N-terminus and the plasma membrane. Deletion, or mutations predicted to disrupt the interaction significantly decreased the β1-subunit induced left shift in the BK channel V0.5max. This suggests that the amphipathic in-plane anchor is critical for functional coupling of β1-subunits with BK channel α-subunits. In this Thesis, we demonstrated: i) palmitoylation of the α-subunit S0-S1 loop controls surface membrane expression of BK channels, and also controls functional regulation by β1, but not β4-subunits; and ii) a potential non-classical amphipathic in-plane anchor in the β1 N-terminus is essential for functional coupling with α- subunits. These studies help us further understand the regulation of BK channels and suggest potential therapeutic targets for various diseases related to dysfunctional BK channels, such as hypertension.

Recently, a number of empirical models have been introduced in the literature for the behavior of direct path used in the design of algorithms for RF based indoor geolocation. Frequent absence of direct path has been a major burden on the performance of these algorithms directing researchers to discover algorithms using multipath diversity. However, there is no reliable model for the behavior of multipath components pertinent to precise indoor geolocation. In this dissertation, we first examine the absence of direct path by statistical analysis of empirical data. Then we show how the concept of path persistency can be exploited to obtain accurate ranging using multipath diversity. We analyze the effects of building architecture on the multipath structure by demonstrating the effects of wall length and wall density on the path persistency. Finally, we introduce a comprehensive model for the spatial behavior of multipath components. We use statistical analysis of empirical data obtained by a measurement calibrated ray-tracing tool to model the time-of- arrival, angle-of-arrival and path gains. The relationship between the transmitter-receiver separation and the number of paths are also incorporated in our model. In addition, principles of ray optics are applied to explain the spatial evolution of path gains, time-of-arrival and angle-of-arrival of individual multipath components as a mobile terminal moves inside a typical indoor environment. We also use statistical modeling for the persistency and birth/death rate of the paths.