125 leaves ; 25cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1974) from the Dept. of Botany, University of Adelaide

A subtribo Espeletiinae (Asteraceae) representa um exemplo clássico de adaptação em ecossistemas tropicais de altitudes elevadas. No entanto, estudos que combinem diferentes campos de pesquisa ainda são necessários para entender este caso proeminente de radiações adaptativas rápidas nos trópicos. Esta tese fornece uma abordagem multidisciplinar combinando informação metabolômica, biogeográfica, taxonômica, evolutiva, química, molecular e ecológica, para um estudo aprofundado da subtribo Espeletiinae e do seu gênero irmão Smallanthus. Através de análises metabolômicas baseadas em cromatografia líquida de ultra-alta eficiência acoplada a espectrometria de massas, nós fornecemos, pela primeira vez, evidências metabolômicas de segregação alopátrica em Espeletiinae e evidência metabolômica apoiando a possível segregação do gênero Espeletia em dois gêneros diferentes com distintas impressões digitais metabólicas. Em combinação com a filogenia molecular da subtribo e amplificações por PCR, demonstramos que a evolução dos caracteres químicos em Espeletiine seguiu cenários complexos de mudança química com alguns caracteres representando sinapomorfias químicas e outros representando múltiplos ganhos e perdas, implicando em evolução convergente. Por fim, analisando os padrões de expressão dos principais genes envolvidos na biossíntese de ácidos clorogênicos, flavonoides e lactonas sesquiterpênicas, em combinação com análises metabolômicas e informações ambientais, relatamos a regulação ambiental e de desenvolvimento do metabolismo secundário de Smallanthus sonchifolius, fornecendo informações relevantes para o entendimento dos fatores regulatórios e possíveis papéis adaptativos dos metabólitos secundários em táxons andinos. Em conclusão, esta tese fornece uma compreensão holística de uma linhagem que representa um exemplo clássico de radiações adaptativas rápidas nos Andes tropicais, abrindo uma nova perspectiva intrigante de pesquisa em outros grupos / The subtribe Espeletiinae (Asteraceae) represents a classic example of adaptation in tropical high-elevation ecosystems. However, studies bringing different research fields are still necessary to understand this prominent case of rapid adaptive radiations in the tropics. This dissertation provides a multidisciplinary approach combining metabolomic, biogeographic, taxonomical, evolutionary, chemical, molecular and ecological information, for an in-depth study of the subtribe Espeletiinae and its sister genus Smallanthus. Through metabolomic analyses based on ultrahigh-performance liquid chromatography-mass spectrometry, we provide, for the first time, metabolomic evidence of allopatric segregation in Espeletiinae and metabolomic evidence supporting a putative segregation of the genus Espeletia in two different genera with distinctive metabolic fingerprints. In combination with the molecular phylogeny of the subtribe and PCR amplifications, we also demonstrate that the evolution of chemical traits in Espeletiinae followed complex scenarios of chemical change with some traits representing chemical synapomorphies and other traits being gained and lost multiple times implying convergent evolution. Lastly, by analyzing the expression patterns of key genes involved in the biosynthesis of chlorogenic acids, flavonoids and sesquiterpene lactones, in combination with metabolomic analyses and environmental information, we report the developmental and environmental regulation of the secondary metabolism of Smallanthus sonchifolius, providing relevant information towards the understanding of the regulatory factors and possible adaptive roles of secondary metabolites in Andean taxa. In conclusion, this dissertation provides a holistic understanding of a lineage representing a classic example of rapid adaptive radiations in the tropical Andes, opening an intriguing new perspective of research in other groups

We present study of the synthesis and reactions of an analog of the meteoritic mineral schreibersite with formula (Fe,Ni)3P, believed to be a prebiotic source of reactive phosphorus that may have prompted the formation of phosphorylated biomolecules near the time of the origin of life (Pasek and Lauretta, 2005). The mineral was synthesized by mixing stoichiometric proportions of elemental iron, nickel and phosphorus and heating in a tube furnace at 820°C for approximately 235 hours under argon or under vacuum, a modification of the method of Skála and Drábek (2002). The mineral was characterized using X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), micro-raman spectroscopy and electron microprobe analysis (EMPA). Characterization indicates that both schreibersite, with approximate formula Fe2NiP and the mineral nickel-phosphide, FeNi2P were synthesized. In addition to characterization of the solid product, the reactions of the synthetic schreibersite were investigated to determine the similarity between these and prior work done with Fe3P. Synthetic schreibersite was corroded in several solutions: seawater and sulfidic water under both oxic and anoxic conditions. After corrosion, the solutions were analyzed using phosphorus nuclear magnetic resonance spectroscopy (31P NMR) and high performance liquid chromatography attached to an inductively coupled plasma mass spectrometer (HPLC-ICP-MS) to determine phosphorus speciation as well as concentrations of phosphorus present in solution. As expected from previous studies, the NMR and HPLC-ICP-MS results indicated the presence of orthophosphate, phosphite, pyrophosphate and hypophosphate in the corrosion solutions (Pasek and Lauretta, 2005). The HPLC-ICP-MS results indicate that the extent of corrosion of the mineral—measured by the concentration of phosphorus released—depends on the ionic strength of the solution, as well as the presence or absence of the chelating agent. Finally, we report the successful phosphorylation of a potentially prebiotic molecule—choline—using synthesized schreibersite.

Este trabalho constituiu-se da análise de abundâncias químicas de nebulosas planetárias localizadas na conexão bojo-disco, onde se dá o encontro das características do bojo, tais como a diversidade de abundâncias, com as do disco, tais como o limite interno do gradiente radial de abundâncias. Em particular, o estudo de nebulosas planetárias nesta região traz informações importantes a respeito das abundâncias de elementos tais como He, O, Ne, Ar, S e de sua evolução associada à evolução das estrelas de massa intermediária. Novas abundâncias foram derivadas a partir de observações espectrofotométricas no telescópio Perkin-Elmer de 1.60 m do Laboratório Nacional de Astrofísica (LNA) em Minas Gerais, Brasil. Foram observadas nebulosas planetárias selecionadas através da localização na direção do centro da Galáxia, diâmetro angular no óptico e fluxo em rádio. A comparação entre as abundâncias obtidas neste trabalho com outros trabalhos da literatura mostrou que as distribuições das abundâncias são compatíveis. Para o estudo da distribuição das abundâncias na conexão utilizou-se as escalas de distância de Maciel & Pottasch (1980), Cahn et al. (1992) e Zhang (1995). A separação das nebulosas planetárias do bojo e do disco mostrou que em média as do bojo apresentam menores abundâncias se comparadas as disco interno, para as escalas de Cahn et al. (1992) e Zhang (1995). Contudo esta separação não é superior aos erros na obtenção das abundâncias, indicando apenas uma tendência. Através deste estudo encontrou-se uma distância de separação entre as propriedades químicas destas regiões. Para a primeira escala esta distância é de 2.9 kpc e para a segunda de 1.5 kpc. Sendo que o valor de 2.9 kpc concorda com resultados independentes. A escala de Maciel & Pottasch (1980) não apresentou resultados conclusivos a respeito da distribuição das abundâncias entre estas estruturas. / This project consisted in a spectrophotometric investigation of planetary nebulae located at the bulge-disk connection of the Milk Way, where the bulge and disk characteristics such as chemical and kinematic properties should intersect. In particular, the study of planetary nebulae in the bulge-disk connection brings important informations about the chemical abundances of elements such as He,N,O,S,Ar,Ne and the evolution of these abundances, associated with the evolution of intermediate-mass stars, as well as for the chemical evolution of the Galaxy. New abundances were derived from spectrophotometric observations at the Perkin-Elmer 1.6 m telescope of Laboratório Nacional de Astrofísica - Brazil. The objects were selected according to their location toward the Galactic center, angular diameter, and radio flux. The data show a good agreement with some other results in the literature, in the sense that the distribution of the abundances is similar to those works. Statistical distance scales from Maciel & Pottasch (1980), Cahn et al. (1992), and Zhang (1995) were used to study the distribution of chemical abundances in the bulge-disk connection. Making use of Cahn et al. (1992) and Zhang (1995) scales, the separation between PNe belonging to the disk and bulge showed that on the average those from the bulge have a slight underabundance compared to those from the inner disk. Nevertheless this separation is not larger than the errors in the abundance determinations, showing only a tendency. This study allowed to find the distance in which the chemical properties of these regions are distinct. For the former scale the distance is 2.9 kpc and for the latter is 1.5 kpc. The value of 2.9 kpc agree with other results for the disk-bulge separation. The same study with Maciel & Pottasch (1980) distance scale did not show any conclusive result about the distribution of chemical abundances between these structures.

Šio darbo tikslas buvo ištirti Galaktikos storojo disko žvaigždžių cheminę evoliuciją. Devynių F-G-K spektrinės klasės žvaigždžių spektrai buvo gauti SOFIN spektrografu su Šiaurės šalių teleskopu. Iš spektrų nustačiau pagrindinius žvaigždžių atmosferų fizikinius parametrus: efektinę temperatūra Teff., gravitacinį pagreitį žvaigždės paviršiuje lg g, mikroturbulencijos greitį vt. ir deguonies, geležies, α, r- ir s-procesų elementų gausas devyniose storojo Galaktikos disko žvaigždėse. Rezultatus palyginau su kitų mokslininkų grupių darbais: tai Tautvaišienės ir kt. darbas (2001 m.); Bensby ir kt. darbai (2004, 2005, 2007 m.); Reddy ir kt. darbas (2006 m.). Geležies gausai [Fe/H] esant intervale nuo ≈ –0,9 iki ≈ –0,3 dešimtųjų žvaigždės, pasižyminčios storojo Galaktikos disko kinematika, yra turtingesnės deguoniu, magniu ir alfa-proceso elementais, nei plonojo disko žvaigždžių populiacija. Kitų cheminių elementų gausos storajame ir plonajame Galaktikos diskų žvaigždėse yra panašios. / The aim of this research is to study the Galactic thick disk chemical evolution. Using high-resolution spectra I have derived the chemical composition of a sample of Galactic thick disk stars. High resolution spectra of 9 F-G-K spectral type stars in the Galactic thick disk have been obtained by the SOFIN spectrograph on the Nordic Optical Telescope. From these stellar spectra I determined the basics stellar atmosphere parameters: effective temperatures Teff [K], surface gravities lg g, microturbulent velocities vt [km/s], and abundances of oxygen, iron, α, r- and s-process elements in the Galactic thick disk. The results are similar to those of other investigations: Tautvaišienė et al., 2001; Bensby et al., 2004, 2005, 2007; Reddy et al., 2006. For a given [Fe/H] the stars with kinematics typical of the thick disk are more enriched in oxygen and α- process elements (Mg, Si, Ca, Ti) than the stars with kinematics typical of the thin disk. The abundance of other elements (Al, Na, Cr, Ni, V, Sc, Mn, Co, Cu, Y, Zr, Ba, La and Nd) is similar for the two disks. The abundance ratios of oxygen, α, r- and s-process elements to iron provide further evidence that the thick disk star population have a different chemical history compared to the thin disk. The stars formation rate was probably higher in the Galactic thick disk than in the Galactic thin disk.

Using a combination of optical and near-infrared photometry, we have studied both the resolved and integrated stellar populations for a sample of Virgo cluster galaxies spanning the full range of galaxian parameters. The derived stellar population properties are compared against galaxy structural and environmental measures to gauge the importance of these factors in establishing galaxy star formation histories and chemical evolution. Although galaxy colours do not uniquely probe a galaxy's star formation history, meaningful results may be obtained if considered in a relative sense. We find that colour profiles reflect variations in both stellar age and metallicity within galaxies. We also uncover systematic variations in colour gradients, and thus age/metallicity gradients, along the Hubble sequence, such that age and metallicity gradients become increasingly negative toward later Hubble types. However, only weak correlations exist between galaxies' stellar populations and their structure and environment. The correlations we find suggest that the star formation histories of gas-rich galaxies are strongly influenced by gas removal within the cluster, while their chemical evolution is due to a combination of stellar mass-dependent enrichment and outflow retention. The assembly of gas-poor giant galaxies is consistent with a hierarchical scenario wherein gas-rich mergers dominate by number. Gas-poor dwarfs differ from the giants, however, appearing as the product of environmentally-driven evolution. Spiral galaxies bridge the dwarf-giant gap, whereby merging and gas-stripping signatures are imprinted in their stars. Early-type spirals seem to have fallen into the cluster sooner than the later types, thereby ceasing star formation in their disks at earlier epochs. The bulges of both types, however, appear to have grown via merging. The nature of this merging (minor versus major) remains unknown. Irregular galaxies exhibit signs of a recent gravitational encounter that has redistributed both their stars and gas, the latter of which caused recent star formation. / Thesis (Master, Physics, Engineering Physics and Astronomy) -- Queen's University, 2009-08-25 14:12:46.48

Damped Lyman α systems (DLAs) are useful probes of the chemical enrichment of the universe as they provide accurate abundance measurements of many chemical species. Using a sample of 30 DLAs (with large metal column densities) observed with the High Resolution Echelle Spectrometer on the Keck I telescope, the abundances of several elements (i.e. iron, zinc, chromium, silicon, sulphur, phosphorus, manganese, and boron) are derived and presented. A comparison is drawn between the abundances from these metal-rich DLAs with literature samples encompassing the largest compilation of high resolution observations of other DLAs, and stars from the Milky Way and its satellite galaxies to understand the astrophysical nature of DLAs. Furthermore, the first ever extragalactic study of boron is presented. Using the sample of 30 metal-rich DLAs, two 3σ detections and one near detection 2.97σ) were found. From the comparison of [B/O] and, for the first time, [B/S], with studies in the Milky Way, there appears to be an excess of boron relative to its parent nucleus (oxygen) in these three DLA systems, suggesting that there may be a higher cosmic ray flux in DLAs than in the Milky Way. / Graduate / 0606

This thesis presents observations and analysis relating to the understanding of processes that govern the formation and evolution of low mass galactic systems. In particular we have focused on separating out the contribution to the chemical and dynamical evolution of dwarf galaxies due to solely secular (internal) processes compared to external effects from the local environment a galaxy resides in. Our observational data focus on an extremely isolated dwarf galaxy, WLM, which we demonstrate has had a uniquely quiescent tidal history, thereby making it an excellent test case for such a study. With spectroscopic and photometric observations of the resolved stars and neutral gas in WLM we have been able to characterize the chemical, structural and kinematic properties of this gas rich dwarf galaxy. As WLM has not been subject to strong tidal or ram-pressure stripping of its stellar and gaseous populations, we have been able to compare the dynamical evolution and chemical history of WLM to theoretical models which are environment independent. A differential comparison of WLM to more environmentally processed dwarf galaxies in the Local Group has revealed that WLM's structural and dynamical state is far from the idealized picture of dIrrs as thin gas-rich rotating systems. The stellar component of WLM shows equal parts rotation and dispersion, and both the gaseous and stellar structural properties show an intrinsically thick axisymmetric configuration. The time evolution of the random (dispersion) component of the stellar orbital energy shows an increase with stellar age, which we show is consistent with secular processes alone - such as disk heating from giant molecular clouds and dark matter substructure. While the degree to which the thick structural and dynamically hot configuration for WLM is surprising, its chemical properties show remarkably consistent values with other galaxies of the same halo mass. Comparing the spatial chemical trends in WLM with other dwarf galaxies we identify a correlation between the strength of the radial abundance gradients and the angular momentum content of dwarf galaxies in the Local Group. Finally using a large sample of chemical abundance measurements in the literature for dwarf galaxies and star clusters, we demonstrate that their distributions of chemical elements all exhibit a binomial form, and use the statistical properties of the distributions to identify a new metric for differentiating low luminosity stellar systems. We further apply a simple binomial chemical evolution model to describe the self-enrichment and pre-enrichment in the two classes of objects, and suggest how this may be used to place constraints on the formation environments of globular clusters in particular. / Graduate

Damped Lyman α systems (DLAs) are useful probes of the chemical enrichment of the universe as they provide accurate abundance measurements of many chemical species. Using a sample of 30 DLAs (with large metal column densities) observed with the High Resolution Echelle Spectrometer on the Keck I telescope, the abundances of several elements (i.e. iron, zinc, chromium, silicon, sulphur, phosphorus, manganese, and boron) are derived and presented. A comparison is drawn between the abundances from these metal-rich DLAs with literature samples encompassing the largest compilation of high resolution observations of other DLAs, and stars from the Milky Way and its satellite galaxies to understand the astrophysical nature of DLAs. Furthermore, the first ever extragalactic study of boron is presented. Using the sample of 30 metal-rich DLAs, two 3σ detections and one near detection 2.97σ) were found. From the comparison of [B/O] and, for the first time, [B/S], with studies in the Milky Way, there appears to be an excess of boron relative to its parent nucleus (oxygen) in these three DLA systems, suggesting that there may be a higher cosmic ray flux in DLAs than in the Milky Way. / Graduate / 0606

Este trabalho constituiu-se da análise de abundâncias químicas de nebulosas planetárias localizadas na conexão bojo-disco, onde se dá o encontro das características do bojo, tais como a diversidade de abundâncias, com as do disco, tais como o limite interno do gradiente radial de abundâncias. Em particular, o estudo de nebulosas planetárias nesta região traz informações importantes a respeito das abundâncias de elementos tais como He, O, Ne, Ar, S e de sua evolução associada à evolução das estrelas de massa intermediária. Novas abundâncias foram derivadas a partir de observações espectrofotométricas no telescópio Perkin-Elmer de 1.60 m do Laboratório Nacional de Astrofísica (LNA) em Minas Gerais, Brasil. Foram observadas nebulosas planetárias selecionadas através da localização na direção do centro da Galáxia, diâmetro angular no óptico e fluxo em rádio. A comparação entre as abundâncias obtidas neste trabalho com outros trabalhos da literatura mostrou que as distribuições das abundâncias são compatíveis. Para o estudo da distribuição das abundâncias na conexão utilizou-se as escalas de distância de Maciel & Pottasch (1980), Cahn et al. (1992) e Zhang (1995). A separação das nebulosas planetárias do bojo e do disco mostrou que em média as do bojo apresentam menores abundâncias se comparadas as disco interno, para as escalas de Cahn et al. (1992) e Zhang (1995). Contudo esta separação não é superior aos erros na obtenção das abundâncias, indicando apenas uma tendência. Através deste estudo encontrou-se uma distância de separação entre as propriedades químicas destas regiões. Para a primeira escala esta distância é de 2.9 kpc e para a segunda de 1.5 kpc. Sendo que o valor de 2.9 kpc concorda com resultados independentes. A escala de Maciel & Pottasch (1980) não apresentou resultados conclusivos a respeito da distribuição das abundâncias entre estas estruturas. / This project consisted in a spectrophotometric investigation of planetary nebulae located at the bulge-disk connection of the Milk Way, where the bulge and disk characteristics such as chemical and kinematic properties should intersect. In particular, the study of planetary nebulae in the bulge-disk connection brings important informations about the chemical abundances of elements such as He,N,O,S,Ar,Ne and the evolution of these abundances, associated with the evolution of intermediate-mass stars, as well as for the chemical evolution of the Galaxy. New abundances were derived from spectrophotometric observations at the Perkin-Elmer 1.6 m telescope of Laboratório Nacional de Astrofísica - Brazil. The objects were selected according to their location toward the Galactic center, angular diameter, and radio flux. The data show a good agreement with some other results in the literature, in the sense that the distribution of the abundances is similar to those works. Statistical distance scales from Maciel & Pottasch (1980), Cahn et al. (1992), and Zhang (1995) were used to study the distribution of chemical abundances in the bulge-disk connection. Making use of Cahn et al. (1992) and Zhang (1995) scales, the separation between PNe belonging to the disk and bulge showed that on the average those from the bulge have a slight underabundance compared to those from the inner disk. Nevertheless this separation is not larger than the errors in the abundance determinations, showing only a tendency. This study allowed to find the distance in which the chemical properties of these regions are distinct. For the former scale the distance is 2.9 kpc and for the latter is 1.5 kpc. The value of 2.9 kpc agree with other results for the disk-bulge separation. The same study with Maciel & Pottasch (1980) distance scale did not show any conclusive result about the distribution of chemical abundances between these structures.