The efficacy of a novel carbohydrate extraction procedure was investigated with methylation analysis and alditol acetate method by Gas Chromatography-Mass Spectrometry. A published extraction procedure for β-glucans was compared to one developed in house. Both procedures gave a dominant glucose peak in the Gas chromatogram indicative of successful β-glucan isolation. Further linkage studies showed four linkage positions for β-glucans isolated with the published method; terminal, 1,3-linkage, 1,6-linkage and 1,3,6-linkage, while β-glucans isolated using the new method showed six linkage positions; terminal, 1,3-linkage, 1,6-linkage, 1,4-linkage, 1,2,3-linkage and 1,3,6-linkage. Diminishing β-glucan linkage peaks in the chromatogram for the published method indicated structure degradation. The results for mannan isolated with 50 mM base gave mannose as a dominant component compared to mannan isolated with 50 mM acid. Base extracted mannan also indicated a good yield of mannan in hyphal form of Candida albicans. This has not been reported with other published isolation methods.

Using radiotracking techniques in Kostelecký les, we studied spatial activity and habitat preferences of three cryptic whiskered bats (Myotis alcathoe, Myotis brandtii, Myotis mystacinus). We examined whether the species emerged from roost at different times. We researched home range (MCP) size and feeding sites (95% Kernel, 50% Kernel), and the distances travelled from the roost. Using a compositional analysis, we evaluated what habitat of the home range and feeding sites Myotis alcathoe and Myotis mystacinus prefer.

Using radiotracking techniques in Kostelecký les, we studied spatial activity and habitat preferences of three cryptic whiskered bats (Myotis alcathoe, Myotis brandtii, Myotis mystacinus). We examined whether the species emerged from roost at different times. We researched home range (MCP) size and feeding sites (95% Kernel, 50% Kernel), and the distances travelled from the roost. Using a compositional analysis, we evaluated what habitat of the home range and feeding sites Myotis alcathoe and Myotis mystacinus prefer.

The development and implementation of effective species and population-specific management strategies requires population-specific information. To demonstrate the relative extirpation risk associated with various road mortality scenarios for a population of Blanding’s turtles at Canadian Nuclear Laboratories in Chalk River, Ontario, a Population Viability Analysis was conducted. Road mortality of two adult females every ten years resulted in population extirpation within 200 years relative to a stable population not experiencing road mortality. To accommodate informed decision-making for the management of this species at risk, the movement patterns and habitat selection of this Blanding’s turtle population were described. There was no significant difference between males and females in distance moved between relocations in either the spring or the summer, but turtles moved greater distances in the spring than in the summer. Annual and seasonal home range size did not differ between the sexes or between spring and summer periods. A compositional analysis indicated Blanding’s turtles preferred marsh habitats over bog, swamp, lake, and upland. Matched-paired logistic regression was used to determine selection of microhabitat features, such as type of vegetation, in the spring and summer. Turtles preferred sites with warmer air temperatures, shallower water, a higher availability of open water, and greater coverage of emergent and floating vegetation types in the spring period. In the summer period, turtles preferred sites characterized by cooler, deeper water, a higher availability of open water, and greater coverage of emergent and floating vegetation types. This population of Blanding’s turtles appears to be relatively small and the continued threat of road mortality indicates a delicate situation for its persistence. Considering seasonally preferred habitats will best inform management decisions for seasonal work restrictions and future development plans.

Component-based software development has emerged as a promising approach for developing complex software systems by composing smaller independently developed components into larger component assemblies. This approach offers means to increase software reuse, achieve higher flexibility and shorter time-to-market by the use of off-the-shelf components (COTS). However, the use of COTS in safety-critical system is highly unexplored. This thesis addresses the problems appearing in component-based development of safety-critical systems. We aim at efficient reasoning about safety at system level while adding or replacing components. For safety-related reasoning it does not suffice to consider functioning components in their intended environments but also the behaviour of components in presence of single or multiple faults. Our contribution is a formal component model that includes the notion of a safety interface. It describes how the component behaves with respect to violation of a given system-level property in presence of faults in its environment. This approach also provides a link between formal analysis of components in safety-critical systems and the traditional engineering processes supported by model-based development. We also present an algorithm for deriving safety interfaces given a particular safety property and fault modes for the component. The safety interface is then used in a method proposed for compositional reasoning about component assemblies. Instead of reasoning about the effect of faults on the composed system, we suggest analysis of fault tolerance through pair wise analysis based on safety interfaces. The framework is demonstrated as a proof-of-concept in two case studies; a hydraulic system from the aerospace industry and an adaptive cruise controller from the automotive industry. The case studies have shown that a more efficient system-level safety analysis can be performed using the safety interfaces.

Microalgae are gaining more attention for several reasons such as being potential producers of sustainable fuel, for use as health supplements and in skincare. Simris Alg is a Swedish company that produces Omega 3 supplements from a primary producer of these fatty acids - the algal diatom Phaeodactylum tricornutum, which is a sustainable alternative to Omega 3 derived from fish. Omega 3 fatty acids constitute a small fraction of the total algal biomass, and to increase profitability and utilize all of the biomass, the purpose of this thesis project is to present potential applications for the residual material that is left after oil extraction. A general composition study was made of Simris Alg algal residue material, and results are compared to those found in previous studies of P. tricornutum biomass. An optimization of the fractionation is needed to separate the storage carbohydrate chrysolaminarin and cell wall component glucuronomannan, followed by analysis for confirmation. Also, it would be interesting to separate chitin, if there is any, since despite the presence of chitin synthases, it is unclear whether the diatom actually produces chitin. When gathering information, no actual experimental characterization of carbohydrate active enzymes involved in synthesis of the main carbohydrates investigated were found. Such information would be useful to increase production of the carbohydrate of interest, if valuable applications are found. Potential applications of various cell components, such as carbohydrates, in skincare would be interesting to investigate, as well as optimizing fucoxanthin extraction for use as an additional high value product next to Omega 3.

The master thesis Bedřich Kocek and Czech Press Photography is concerned with the life and work of Bedřich Kocek - Czech photojournalist who worked in the renowned journal Svět v obrazech (translated as World in Pictures) for over thirty years. The theoretical part specifies the genre of journalistic photography and its development from the turn of the 19th and 20th centuries to the 1980s. Furthermore, the development of the journal Svět v obrazech serves as an example for outlining the media and political context in Czechoslovakia since the end of World War II, when the periodical was established, until the 1980s, when Bedřich Kocek left the profession of a photojournalist. An especially valuable and vital part of the thesis is a chapter about the life of Bedřich Kocek, which has not been previously researched and described in detail. That section is based on personal interviews with the photographer using the oral history method. Moreover, the thesis explores the photographic work of Bedřich Kocek through the use of compositional analysis as well as the characteristic features of his pictures, which were part of the reports produced for Svět v obrazech.

Migration of animals has been studied for decades and has included everything from large terrestrial and pelagic mammals traveling thousands of kilometers to many types of birds flying through several countries to insects going through multiple life cycles in a single migration. The migration of bats has been studied in broad terms to gather coarse information such as distance traveled, connecting summer and winter habitat, and a general understanding of timing. However, only recently have researchers begun to understand the specifics of bat migration including physiology and fine resolution behavior. Using nine years of spring migration data collected on VHF radio-tagged federally endangered female Indiana bats (Myotis sodalis), I predicted behavior based on previous migration studies for bats that used various methods such as band recovery data, stable hydrogen isotopes, and automated telemetry. My project used aerial telemetry to collect location points while tracking individual bats throughout the migration. I describe distances traveled, how much time bats spent in migration, traveling and foraging behavior during migration, and how weather affects bat behavior. In addition, this project resulted in the location of 17 previously unknown maternity colonies for the species including the southernmost colony known to date. Next, I used temperature data collected from individual bats along with ambient temperature to determine how air temperature affects specific bat behavior. I calculated air temperature thresholds for use of torpor in Indiana bats and described how air temperature affects transition states between torpor and normothermy. Finally, I determined landscape preferences for migrating bats during travel and foraging. Indiana bats tend to migrate in a direct path from hibernaculum to summer grounds and use the habitat in the proportion that it is available. Although bats used forested cover when possible, they did travel in open areas (e.g., across agricultural fields) when necessary to continue along the intended trajectory. This data set is the first to track individual nocturnally migrating bats via aerial telemetry for the entirety of the spring migration journey. It provides specific information about how far Indiana bats travel and how long they are migrating across the landscape, illustrates a web of connections between summer and winter habitat, describes the effect of weather on bat behavior, and provides landscape use information that can be useful for land managers and developers.

This Ph.D. research project targets Cooper Basin oil reservoirs of very low permeability (approximately 1mD) where injectivities required for water flooding are not achievable. However, the use of injection gases such as CO₂ would not have injectivity problems. CO₂ is abundant in the region and available for EOR use. CO₂ was compared to other CO₂-rich injection gases with a hydrocarbon content including pentane plus components. While the effect of hydrocarbon components up to butane have been investigated in the past, the effect of n-pentane has on impure CO₂ gas streams has not. One particular field of the Cooper Basin was investigated in detail (Field A). However, since similar reservoir and fluid characteristics of Field A are common to the region it is expected that the data measured and developed has applications to many other oil reservoirs of the region and similar reservoirs elsewhere. The aim of this Ph.D. project is to determine the applicability of CO₂ as an injection gas for Enhanced Oil Recovery (EOR) in the Cooper Basin oil reservoirs and to compare CO₂ with other possible CO₂-rich injection gases. The summarised goals of this research are to: • Determine the compatibility of Field A reservoir fluid with CO₂ as an injection gas. • Compare CO₂ to other injection gas options for Field A. • Development of a correlation to predict the effect of nC₅ on MMP for a CO₂- rich injection gas stream. These goals were achieved through the following work: • Extensive experimental studies of the reservoir properties and the effects of interaction between CO₂-rich injection gas streams and Field A reservoir fluid measuring properties related to: • Miscibility of the injection gas with Field A reservoir fluid • Solubility and swelling properties of the injection gas with Field A reservoir fluid • Change in viscosity-pressure relationship of Field A reservoir fluid due to addition of injection gas • A reservoir condition core flood experiment • Compositional simulation of the reservoir condition core flood to compare expected recoveries from different injection gases • Development of a set of Minimum Miscibility Pressure (MMP) measurements targeted at correlating the effect of nC₅ on CO₂ MMP. The key findings of this research are as follows: • Miscibility is achievable at practical pressures for Field A and similar reservoir fluids with pure CO₂ or CO₂-rich injection gases. • For Field A reservoir fluid, viscosity of the remaining flashed liquid will increase at pressures below ~2500psi due to mixing the reservoir fluid with a CO₂-rich injection gas stream. • Comparison of injection gases showed that methane rich gases are miscible with Field A so long as a significant quantity of C₃+ components is also present in the gas stream. • There is a defined trend for effect of nC₅ on MMP of impure CO₂. This trend was correlated with an error of less than 4%. • Even though oil composition is taken into account with the base gas MMP, it still affects the trend for effect of nC₅ on MMP of a CO₂-rich gas stream. • An oil characterisation factor was developed to account for this effect, significantly improving the results, reducing the error of the correlation to only 1.6%. The significance of these findings is as follows: • An injection pressure above ~3000psi should be targeted. At these pressures miscibility is achieved and the viscosity of the reservoir fluid injection gas mix is reduced. • CO₂ should be compared to gases such as Tim Gas should after considering the cost of compression, pipeline costs and distance from source to destination will need to be considered. • The addition of nC₅ will reduce the MMP and increase the recovery factor, however the cost of the nC₅ used would be more than the value of increased oil recovered. • The developed correlation for the effect of nC₅ on impure CO₂ MMP can be used broadly within the limits of the correlation. • Further research using more oils is necessary to validate the developed oil characterisation factor and if successful, using the same or similar method used to improve other correlations. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1369016 / Thesis (Ph.D.) -- University of Adelaide, Australian School of Petroleum, 2009.

Spanish, as a V(erb)-framed language (Talmy 1985), is expected to lexicalize the path of motion in the verb and manner in some satellite when it comes to the description of motion events. Nonetheless, it shows mixed properties (e.g. Aske 1989, Berman & Slobin 1994). All manner of motion verbs can take a path satellite introduced by the prepositions "hacia" and "hasta", and yet only some can take a path satellite introduced by the preposition "a." I claim that goal XPs introduced by "hasta" and "hacia" are adjuncts, whereas "a" is an argument marker. In order to capture the intermediacy of a verb’s ability to take a goal XP, I classify manner of motion verbs according to a three-way distinction that takes into account whether they encode path categorically, overwhelmingly, or only sometimes, and whether they lexically reject the notion of a goal. Finally, I posit verb coercion—under certain semantic and pragmatic conditions—of manner of motion verbs that strongly or categorically favor displacement in order to express a goal. These semantic/pragmatic influential factors are reduced to (i) degree of manner and (ii) degree of goal-orientedness. / text