The use of many of South Africa’s medicinal plants has shown marked increase with over 27 million users in South Africa alone. Most plants are still being unsustainably wild-harvested, a major concern for biodiversity conservation. Commercial interest in certain more commonly-used species has increased, with potential to cultivate medicinal plants on a more sustainable basis. Focus has shifted from conventional use of synthetic fertilisers, pesticides and fungicides to more organic methods of plant propagation. Aqueous extract derived from earthworm composted food waste (vermitea) was used to study the germination and rooting success of selected species. Also survival and growth performance of selected plants grown in a medium amended with commercial NPK fertiliser was compared to those grown in the same medium amended with compost and to those grown in the same medium amended with compost with weekly applications of vermitea. No change in germination success was noted. Vermitea showed promising results on the rooting of cuttings. The application of NPK improved growth performance (biomass) significantly for all species tested. However, they had lower root:shoot ratios as well as lower survival rates compared to plants under the compost and compost/vermitea treatments. The improved survival of these plants highlights the potential of these organic treatments on the propagation of selected medicinal plants.

Memoria para optar al Título Profesional de Ingeniero en Recursos Naturales Renovables / El Protocolo de Kioto establece compromisos de reducción de emisiones de gases de efecto invernadero a los países desarrollados, lo que ha generado un mercado de emisiones de carbono. Las emisiones de metano provenientes de los rellenos sanitarios, pueden ser reducidas si parte de los residuos que se destinan al relleno sanitario son desviadas a un proceso aeróbico, como el compostaje. El propósito de este estudio fue determinar la viabilidad técnica de un sistema de compostaje y efectuar un análisis económico preliminar que permita determinar la conveniencia al ingresar al mercado de los bonos de carbono.

Thesis ((MSc. Agriculture (Agronomy)) -- University of Limpopo, 2015 / An experiment was conducted to evaluate the effect of vermicompost on cabbage growth and yield. Vermicompost (VC) was applied at 2500 kg/ha and was compared to one organic {vita grow (VG) [N:P:K 2:3:2 (16)] and one chemical fertiliser (CF) (N:P:K 2:3:2(22)]} which were both applied at 1500 kg/ha. The other treatments were prepared as mixtures of the three fertilisers (i.e. vermicompost and vita grow; vita grow and chemical fertiliser as well as vermicompost and chemical fertiliser) at 50% recommended rate, with no fertiliser treatment as a control. The trial was laid out in a randomised complete block design with three replicates. Chemical fertiliser achieved the highest chlorophyll content (80.6 nmol/mg) which was significantly higher than the other treatments. Vermicompost and the control had the least chlorophyll content at 58.6 and 55.8 nmol/mg, respectively. VC+VG, CF and CF+VC achieved the highest numbers of marketable heads while the control had the least. VC+VG, CF and CF+VC had more than 60% of the plants producing marketable heads whilst VC and VG had less than 40% of the plants producing marketable heads. The control only had 19.93% of the plants producing marketable heads. Range of marketable heads ranged from 2.82 t/ha for the control to 12.7 t/ha for CF. VC+VG, CF and CF+VC achieved marketable yields above 10.0 t/ha.VC and VG achieved 5.45 and 8.28 t/ha. The results thus show that use of chemical fertiliser or the combinations of VC and VG and that of CF and VC are the ones that can be recommended for cabbage production. Key words: vermicompost, growth, yield, cabbage, fertiliser

>Magister Scientiae - MSc / The complete degradation of recalcitrant lignocellulose biomass into value-added products requires the efficient and synergistic action of lignocellulose degrading enzymes. This has resulted in a need for the discovery of new hydrolytic enzymes which are more effective than commonly used ones. β-xylosidases and β-glucosidases are key glycoside hydrolases (GHs) that catalyse the final hydrolytic steps of xylan and cellulose degradation, essential for the complete degradation of lignocellulose. Functional-based metagenomics has been employed successfully for the identification and discovery of novel GH genes from a metagenome library. Therefore, this approach was used in this study to increase the chances of discovering novel glycoside hydrolase genes from a horse manure metagenomic DNA library constructed in a previous study. Three fosmid clones P55E4, P81G1, and P89A4 exhibiting β-xylosidase activity were found to encode putative glycosyl hydrolases designated XylP55, XylP81, and BglP89. Amino acid sequence analysis revealed that XylP55, XylP81, and BglP89 are members of the GH43, GH39, and GH3 glycoside hydrolase families, respectively. Phylogenetic analysis of XylP81 and BglP89 indicated that these showed relatively low sequence similarities to other homologues in the respective GH families. The enzymes were expressed and purified, and only XylP81 and BglP89 were biochemically characterized. XylP81 (~58 kDa) and BglP89 (~84 kDa) both showed optimum activity at pH 6 and 50℃ and retained 100% residual activity at 55℃ after 1-hour indicating that they are moderately thermostable. XylP81 had high specific activity against 4-nitrophenyl-β-D-xylopyranoside (pNPX; 122 U/mg) with a KM value of 5.3 mM, kcat/KM of 20.3 s-1mM-1, and it showed enzyme activity against α-L-arabinofuranosidase, β-galactosidase, and β-glucosidase activity. BglP89 had a high specific activity for 4-nitrophenyl-β-D-glucopyranoside (pNPG; 133.5 U/mg) with a KM value of 8.4 mM, kcat/KM of 22 s-1mM-1 and also showed α-L-arabinofuranosidase, β-galactosidase, β-glucosidase, and low β-xylosidase activity. BglP89 also showed low hydrolytic activity on cellobiose, β-glucan, and lichenan indicating that it is a broad specificity β-glucosidase. XylP81 retained ~40% activity in the presence of 3 M xylose whilst BglP89 showed considerable glucose tolerance at 150 mM glucose and retained ~46% residual activity. This study reveals two metagenomic derived enzymes (β-xylosidase and β-glucosidase) showing characteristics that could make them potential candidates for lignocellulose biomass degradation in biotechnological and industrial applications.

The objectives of this study were to investigate nutrient retention, intI1 prevalence, and compost maturity rates for poultry litter co-composted with 5, 10, and 20% southern yellow pine biochar and with or without 2% wood vinegar (WV). Samples were collected at 0, 57, and 112 days to measure nitrogen, phosphorus, and potassium (N, P, K) concentrations, microbial counts, pH, moisture content, carbon to nitrogen (C:N) ratio, and intI1 abundance. Composts were aerated once a week and the temperature was also recorded once a week. There was sufficient rainfall so no additional water was added. The results showed that N and P concentrations significantly increased over time in all treatments except 20% biochar and 20% biochar + wood vinegar, while K concentrations significantly decreased. In general, composting with wood vinegar significantly decreased nutrient concentrations; however, all nutrient concentrations were much higher than typical animal manure fertilizers. Increases in biochar level resulted in significantly lower bacteria counts and significantly higher fungi counts. Compost treatments containing wood vinegar had significantly lower bacteria and fungi counts, indicating that southern yellow pine wood vinegar had a biocide effect on microorganisms, and may be not suitable for composting at that application rate. intI1 prevalence was not significantly different among treatments, which may be due to insufficient thermophilic composting. Because thermophilic temperatures were not achieved, the compost was not mature by the end of the study; therefore, compost maturity rates could not be determined.

The solid portion of waste disposal, known as Municipal Solid Waste (MSW) can be landfilled. Landfilling has proved to be a safe, sanitary and economical method of disposal. A by-product from wastewater treatment plants called biosolids is sometimes co-disposed along with MSW in landfills. Recent work at the University of Central Florida has focused on the behavior of the mixture of MSW and biosolids. As an increased amount of waste accumulates in these landfills, it creates a new problem – the geotechnical stability of landfills. In current literature, classical geotechnical testing methods have been followed to find the strength properties of these landfill materials. Furthermore, geotechnical methods of slope stability analyses have been employed to determine the stability of landfill slopes. As these materials have a high organic content, their strength properties may potentially change with time because of the decay of the organic materials. In the present work, an attempt is made to monitor the change in the geotechnical strength properties of the landfill materials as a function of time. Direct shear tests used for soil testing, with some modifications, were performed on cured compost samples of MSW mixed with biosolids. Geotechnical strength properties of these cured samples were compared to those of an artificially prepared mixture of MSW and biosolids, from the published literature. In addition, direct shear tests are also performed to find the interface properties of a geonet with the cured samples to check the role of a geonet in reinforcing the landfill slopes. A slope stability analysis software SLOPE/W is used to analyze the stability of the landfills. Cohesion is observed to decrease with time while the friction angle increases with time. Stability (the factor of safety against failure) of landfill slopes increases with time due to increased effective stresses and increased friction angle, as the organic material decays. This may result in additional subsidence but an increase in the effective shear strength with time. Based on the interface test results and subsequent slope stability analyses, it is found that the inclusion of a geonet improves the slope stability of a landfill. This could be a potential benefit to the landfill as reinforcement if properly placed. Based on the slope stability analysis on landfills with different slopes, it is concluded that the slope stability of a landfill is improved by keeping the slopes less steep.

En agriculture biologique, peu de produits sont autorisés pour améliorer la croissance des plantes. Cette étude qualifie les éléments solubles d’extraits de compost (EC) sur la croissance du soya. En présence ou non d’aération, six composts ont macéré dans l’eau pendant 14 jours et caractérisé. Quatre EC ont été sélectionnés, fortifiés et vaporisés sur le feuillage du soya cultivé dans du sable et traité avec de l’eau ou une solution nutritive. Les éléments solubles des EC évoluent en fonction du temps, dont les nitrates qui diminuent en fonction de l’aération. La teneur en éléments minéraux des EC est optimale après 2 jours et sans aération. L’EC «Fumier de vers» a démontré une augmentation du développement, des biomasses, et du prélèvement en éléments minéraux du soya traité avec une solution nutritive. L’effet bénéfique de cet EC semble lié à des substances de croissance solubles, autres que les éléments minéraux solubles. / In organic agriculture, there are few certified products that can be used to favor plant growth. This study was conducted to determine the soluble compounds from water extract of compost (WEC) that could benefit soybean growth. In the presence or the absence of aeration, six composts were macerated into water up to 14 days. Following the characterization of WEC, four were selected, enriched and applied on foliage of soybean grown in sand and watered with a nutrient solution or water. Over time, most soluble mineral salts went into solution, but N could be lost trough denitrification. To enhance mineral contents and minimize N loss, WEC should be produced within two days. When treated with the nutrient solution, the water extract of vermicompost enhanced soybean development, biomasses, and plant mineral contents. This WEC appears to favor plant growth through the provision of soluble growth substances, other than soluble mineral elements.

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