For a long time, Roman ideal sculptures have primarily been studied within the tradition of Kopienkritik. Owing to some of the theoretical assumptions tied to this practice, several important aspects of Roman visual culture have been neglected as the overall aim of such research has been to gain new knowledge regarding assumed Classical and Hellenistic models. This thesis is a collection of three studies on Roman ideal sculpture. The articles share three general aims: 1. To show that the practice of Kopienkritik has, so far, not produced convincing interpretations of the sculpture types and motifs discussed. 2. To show that aspects of the methodology tied to the practice of Kopienkritik (thorough examination and comparison of physical forms in sculptures) can, and should, be used to gain insights other than those concerning hypothetical Classical and Hellenistic model images. 3. To present new interpretations of the sculpture types and motifs studied, interpretations which emphasize their role and importance within Roman visual culture. The first article shows that reputed, post-Antique restorations may have an unexpected—and unwanted—impact on the study of ancient sculptures. This is examined by tracing the impact that a restored motif ("Satyrs with cymbals") has had on the study of an ancient sculpture type: the satyr ascribed to the two-figure group "The invitation to the dance". The second article presents and interprets a sculpture type which had previously gone unnoticed—The satyrs of "The Palazzo Massimo-type". The type is interpreted as a variant of "The Marsyas in the forum", a motif that was well known within the Roman cultural context. The third article examines how, and why, two motifs known from Classical models were changed in an eclectic fashion once they had been incorporated into Roman visual culture. The motifs concerned are kalathiskos dancers, which were transformed into Victoriae, and pyrrhic dancers, which were also reinterpreted as mythological figures—the curetes. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Accepted. Paper 3: Accepted.</p>

Humans are continuously challenged by harmful endogenous and xenobiotic substances. Detoxification is the ability to neutralise and remove these substances from the body. Glycine N-acyltransferase, EC 2.3.1.13 (GLYAT) is a key enzyme in detoxification. GLYAT catalyses an amino acid (glycine) conjugation reaction in phase II of detoxification. It is expected that, similar to what has been observed in the Cytochrome P450 enzymes, variations within the GLYAT gene may lead to altered enzyme activity that may affect the efficacy of detoxification. The aim of this study was to identify genetic variations within the GLYAT gene of a cohort of individuals whose GLYAT activity has been biochemically characterized. Biochemical profiles of phase I and II detoxification of a number of individuals was screened to select those with possible aberrant GLYAT activity. Eighteen selected individuals agreed to participate in the study. The 23.21 kb GLYAT gene of the participants was amplified in four fragments and sent for pyrosequencing (Roche GS FLX titanium) at Inqaba Biotec. The results were analysed with the Lasergene software package from DNAStar (Madison, Wisconsin, USA). A total of 94 variations were identified from the Next Generation Sequencing data. Of these three found in the exons were known variations and four variations located in the exons were novel. A total of 62 known and 25 novel variations were identified in the introns of the GLYAT gene. Sanger sequencing verified 70.29% (68 in total) of the variation, which included 12 novel variations, of which one is located in exon six. Real-time quantitative PCR (qPCR) experiments were conducted and the data analysed using CopyCaller software to identify copy number variations within the cohort. It was found that participant 17 may have multiple copies of parts of the 3-terminal end of the gene (exons five and six), which might have an effect on GLYAT activity. Variations could possibly affect GLYAT activity, but the data was inconclusive and must be confirmed. Some of the variations could possibly affect GLYAT activity, but no correlation could be made between the variations identified during this study and the cohort’s detoxification ability. Further studies needs to be conducted to establish the effect of the variations in combination with one another on GLYAT activity. If some of these variations affect GLYAT activity such data might shed some light on variations observed between the glycine conjugation ability of individuals. Such information could eventually be of value in treatment of inborn errors of metabolism. / MSc (Biochemistry), North-West University, Potchefstroom Campus, 2014

When offenders commit crime there is the potential that they may leave behind trace amounts of their DNA, even when there has been no apparent body fluid spill. During the examination of crime scenes, scene investigators try to identify areas that may be sampled to locate these traces. Specialist techniques are then required within the laboratory to enable such small amounts to be analysed to obtain a profile. These techniques are referred to as Low Template DNA analysis (LTDNA), of which Low Copy Number DNA (LCN DNA) is one instance. In 2008, following the Omagh Bombing trial, and comments made by Judge Weir, the UK Forensic Regulator commissioned a review of the science of LTDNA analysis. The subsequent report made specific mention of the fact that there was no available information on the success rate of the use of such DNA techniques and that there seemed to be confusion over what constituted a success. The report went on to state that there was no information on where such trace amounts of DNA were likely to be found, or what factors could influence the likelihood of obtaining a trace DNA profile (Caddy, 2008). This research considered the outcomes of LCN DNA analysis from 3,552 samples to try to establish where trace amounts of DNA could be found, whether some areas sampled were more successful in generating profiles than others, and the likelihood of the profiles obtained being of use to a criminal investigation. Analysis of results identified areas that were more successful in generating profiles of use to an investigation and highlighted significant differences in results across a variety of items from which samples were taken. DNA samples taken from items associated with communication such as mobile phones were much more likely to produce a profile useful to a criminal investigation than those taken from fixed surfaces within premises. The results obtained showed that obtaining a DNA profile did not necessarily correlate with the profile being of use to a criminal investigation. This was due to the fact that a large number of these profiles were anticipated eliminations from legitimate sources. Items that produced high numbers of profiles but were anticipated eliminations, and therefore of no value to an investigation, came from items associated with skin samples and clothing. The research went further to identify key factors that affected the profiling rates. Factors that had a positive influence on the ability to obtain a profile included: any area that had been in close proximity to saliva (direct contact was not required); samples that had been recovered from the inside of premises or vehicles and therefore protected from the elements; those that were dry; items that were of a porous nature; and those that had a rough texture. No differences were found between the actual surface materials (plastic, glass, wood, metal), as all showed a propensity to generate profiles. Other factors that were considered but proved to have no effect on the profiling rates included seasonal differences and whether the area targeted for sampling was clearly defined. Items that had had high contact with a victim, were recovered from outside or had been wet, all proved to be less useful to an nvestigation. A further finding of the research was that swabs that had been recovered and stored frozen appeared to deteriorate in their ability to profile. This was particularly notable if they were submitted later than 5 months after recovery. Items stored in dry conditions did not deteriorate in this way. Overall the research can be used to provide investigators with the knowledge of what areas of crime scenes are most likely to yield trace DNA material, the key factors that can affect the likelihood of obtaining a profile, and those areas that are more likely to produce profiles useful to criminal investigations.

We are today experiencing an increasing demand for high energy density storage devices like the lithium-ion battery for applications in portable electronic devices, electric vehicles (EV) and as interim storage for renewable energy. High capacity retention and long cycle life are prerequisites, particularly for the EV market. The key for a long cycle life is the formation of a stable solid-electrolyte interphase (SEI) layer on the surface of the negative electrode, which typically forms on the first cycles due to decomposition reactions at the electrode-electrolyte interface. More control over the surface layer can be gained when the layer is generated prior to the battery operation. Such a layer can be tailored more easily and can reduce the loss of lithium inventory considerably. In this context, water-soluble electrode binders, e.g. sodium carboxymethyl cellulose (CMC-Na) and poly(acrylic acid) (PAA), have proven themselves exceptionally useful. Since the binder is a standard component in composite electrodes anyway, its integration into the electrode fabrication process is easily accomplished. This thesis work investigates the parameters that govern binder distribution in elec-trode coatings, control the stability and electrochemical performance of the elec-trode and that determine the composition of the surface layer. Several commonly used electrode materials (graphite, silicon and lithium titanate) have been applied in order to study the impact of the binder on the electrode morphology and the differ-ent electrode-electrolyte interfaces. The results are correlated with the electrochemi-cal performance and with the SEI composition obtained by in-house and synchro-tron-based photoelectron spectroscopy (PES). The results demonstrate that the poor swellability of these water-soluble binders leads to a protection of the active material, given that the surface coverage is high and the binder evenly distributed. Although on the laboratory scale electrode formu-lations with a high binder content are common, they have little practical use in commercial devices due to the high content of inactive material. As the binder con-tent is decreased, complete surface coverage is more difficult to achieve and the binder distribution is more strongly coupled to the particle-binder interactions during the preparation process. Moreover, it is demonstrated in this thesis how these inter-actions are related to the surface area of the electrode components applied, the surface composition and the electrochemistry of the electrode. As a result of the smaller binder contents the benefits provided by CMC-Na and PAA at the electrode surface are compromised and the performance differs less distinctly from electrodes fabricated with the conventional binder, i.e. poly(vinylidene difluoride) (PVdF). Composites of alloying and conversion materials, on the other hand, typically em-ploy binders in larger amounts. Despite the frequently noted resiliency to volume expansion, which is also a positive side effect of the poor swellability of the binder in the electrolyte, the protection of the surface and the formation of a more stable interface are the major cause for the improved electrochemical behaviour, com-pared to electrodes employing PVdF binders.

Improved survival for women receiving chemotherapy for breast cancer (BC) has been accompanied by the development/persistence of psychoneurological symptoms (PNS) that compromise their quality of life. The biological basis for these PNS is unknown, but could reflect the acquisition of soma-wide chromosomal/epigenetic alterations. An important first step in testing this hypothesis is to determine if somatic genetic/epigenetic changes arise and persist following treatment. To answer this question we longitudinally studied 71 women (ages 23-71) with early-stage BC and collected measures before chemotherapy (baseline), and 4 weeks (mid-chemo); six months (during radiation therapy for a subset of women); and one year following the initiation of chemotherapy. Acquired lymphocyte chromosomal instability (scored by micronuclei frequencies [MNF]) showed a significant increase in post-treatment compared to baseline time-points (p<0.0001), with these increases persisting for at least one year following chemotherapy. Significant predictive associations were observed between MNF and tumor characteristics [luminal B (lower MNF; p=0.0182); triple negative (higher MNF; p=0.0446)], radiotherapy (higher MNF; p=0.0004), the type of chemotherapy received (p=0.0463), race (Caucasians > African Americans; p=0.0037), perceived stress levels (positive-association; p=0.0123), and cognitive flexibility domain measures (positive-association; p=0.0238). Genome-wide acquired methylation changes were also measured in peripheral blood cells, with 1265 sites showing significant differential methylation following chemotherapy. These sites were localized to open sea, shores, shelves, and CpG island sequences and included sites within genes involved in cell cycle, DNA repair, transcription regulation, signal transduction pathways, neuronal regeneration, and immunity. To determine if the genetic/epigenetic alterations acquired in peripheral blood cells correlated with those in tumor cells, BC tumors from 10 participants were analyzed using a genome-wide copy number/targeted mutations (CN/M) microarray. While no clear blood-tumor cell correlations were detected, genome-wide CN/M evaluations showed promise for stratifying tumors. Lastly, in an unrelated project studying a rare case of fetuses in fetu, methylation changes acquired in embryogenesis were shown to be influenced by both environmental and genetic cues. In summary, acquired chromosomal/epigenetic alterations do arise following chemotherapy (and in embryogenesis). Further delineation of these acquired changes could increase our understanding of the biological basis for cancer-related side-effects and help to identify “at risk” individuals.

The market for high-tech services is expanding, and writers will have to create more documents to market these services. Researchers note marked differences between traditional goods marketing and services marketing. A rhetorical framework for high-tech services marketing will give writers a tool for creating effective marketing messages. This study examines the five canons of rhetoric in their classical context, and then examines how the first professional teachers, the Sophists, used rhetoric to promote their services. The canons of rhetoric are then analyzed to show their modern significance. This study also considers visual rhetoric and how writers can use it effectively. This study shows that companies should promote service quality and strong service relationships through the rhetorical element of ethos. This study examines services marketing samples through a visual and verbal rhetorical framework, providing rhetorical insights that writers can use in their work.

Plasmids are small circular DNA molecules within bacterial cells that are separated from the bacterial chromosome and replicate independently. Also, they play a crucial role in the dissemination of antibiotic resistance genes among bacteria through horizontal gene transfer. They can be present in many copies within host cell, which is known as plasmid copy number. Plasmids can regulate their own copy number by different mechanisms. Additionally, the selective pressure can also play a pivotal role in determining plasmid copy number. The presence of antibiotics in the surrounding environment can drive variations of plasmid copy number. In this study, we examined plasmid copy number variations of multidrug resistance plasmids in presence of antibiotics by using EvaGreen® - based multiplexed digital droplet PCR. We could observe that cultures of Klebsiella pneumoniae and Escherichia coli harboring multidrug resistance plasmids grown in presence of sub-MIC concentrations of the antibiotics did not show high variations in plasmid copy numbers. On the other hand, mutants of K. pneumoniae selected for increased antibiotic resistance showed high increases in copy number of a multidrug-resistance plasmid.

Avec ses quatre témoins, le récit épique d’Anseÿs de Gascogne est l’un des succès du Moyen-Âge. C’est une conclusion du Cycle des Lorrains nettement en faveur des comtes du Nord, et Philippe le Bon en a naturellement commandé une mise en prose. La copie de David Aubert datée de 1465 nous est parvenue dans un luxueux manuscrit conservé à Bruxelles (KBR, 9), quatrième volume des Histoires de Charles Martel. La seconde partie du récit – à partir de la bataille en Santerre – sur laquelle s’appuie notre étude, ne peut être considérée comme un simple dérimage. En effet, elle révèle les goûts de la société bourguignonne du XVe siècle, s’organise en chapitres et modernise la langue, le vocabulaire et surtout les techniques littéraires. Nous sommes donc en présence d’un récit davantage ancré dans l’époque de la copie que dans celle de son modèle en vers. La présente édition réunit une description du manuscrit, une étude littéraire commentant la filiation de la prose et du vers, une analyse linguistique ainsi qu’un glossaire, un index des noms propres et une table des proverbes. / The epic tale Anseÿs de Gascogne, which is composed of four reference texts, is one of the best medieval literary achievements. It comes as a conclusion to the Lorraine cycle clearly paying tribute to the counts of the North of France and Philippe le Bon naturally ordered a copy of it in prose. David Aubert’s copy, dating back to 1465, is to be found in a luxurious manuscript kept in Brussels (KBR, 9) in the fourth volume of Histoires de Charles Martel. The second part of the tale – from the Battle of Santerre onwards – on which our study lies should not be considered as a mere conversion from poetry to prose. Indeed, not only does it throw light upon the tastes of the Bourguignon society of the XVth century but it also falls into chapters and modernises the language, the vocabulary and above all, the narrative techniques. The tale is really anchored in the prose tradition rather than in that of verse. This edition gathers a description of the manuscript, a literary study commenting on the connections between prose and verse, a linguistic analysis as well as a glossary, an index of proper nouns and a table of proverbs.

O filme Cópia fiel (2010), de Abbas Kiarostami, configura um objeto estranho dentro da filmografia desse autor iraniano. Ao mesmo tempo que muito dialoga com questões já apresentadas em trabalhos anteriores do diretor, o filme marca uma ruptura, indica um novo caminho. Se sua obra continuamente explorou os cruzamentos entre ficção e realidade, Cópia fiel, por sua vez, parece mergulhar na ficção sem se referir a uma realidade que lhe seja exterior ou contígua. A dramaturgia e as construções formais do filme (enquadramentos, montagem, mise en scène) elaboram uma reflexão conceitual sobre a própria ideia de ficção e representação artística, explorando os conceitos-chave da obra: cópias e originais. Esta dissertação busca, através da análise fílmica, investigar tais procedimentos do filme que competem para tensionar a própria ideia de ficção cinematográfica. Dividimos nosso percurso em três partes para dar conta de distintos aspectos da ficção que o filme aborda, sintetizados nos termos palco, espelho e janela. O palco representa o ponto em que a ficção se coloca como evidentemente separada da realidade: lugar de paradoxos e de fricções entre ator e personagem. O espelho representa as infinitas repetições e duplicações da estrutura do filme e a relação de identificação-projeção do espectador com a obra. Por fim, a janela explora o viés realista do filme, sua vocação \"transparente\", problematizando a possibilidade de se acessar um determinado real, o original de todas as cópias. / The film Certified copy (2010), by Abbas Kiarostami, is a strange object within his filmography. It continues to work on issues presented in the director\'s prior works, but it also represents a rupture, indicating a new path. His works continually explored the entanglements between fiction and reality, whereas Certified copy seems to abolish any perspective on an exterior reality to the film\'s fiction. The dramaturgy and the formal constructions of the film (framings, montage, mise en scène) build a conceptual reflection on the very idea of fiction and artistic representation, exploring key-concepts of the work, such as copies and originals. This dissertation intends to investigate through filmic analyses the procedures that tension the notion of cinematic fiction. The text is divided in three parts, each of them aiming at a particular aspect of the fiction explored by the movie, each compressed into one symbolic notion: stage, mirror and window. The stage represents the strict division between reality and fiction: a place of paradoxes and frictions between the actors and their characters. The mirror investigates the infinite repetitions and duplications within the film structure, and also the spectator\'s process of projection and identification. Finally, the window explores the realistic aspect of the film, its \"transparent\" vocation, questioning the possibility of a true access of the real, the original to every copy.

Chan, Ho Ching. / Thesis M.Phil. Chinese University of Hong Kong 2016. / Includes bibliographical references (leaves 159-166). / Abstracts also in Chinese. / Title from PDF title page (viewed on 15, September, 2016).