Southern Chile is recognized by different international organizations such as UNESCO (United Nations Educational, Scientific and Cultural Organization), and Conservational International as an ecologically sensitive area. The country of Chile is home to one third of the earth’s remaining temperate forests. Monitoring and assessing land cover changes in these forests is important not only to international organizations but to the people of Chile. The lack of multi-temporal studies that evaluate changes in land cover biomass make this study an important one for increasing awareness of how the evolution of the landscape affects environmental planning and development of legal precedents aimed at protecting this rich ecological habitat. The results of this study revealed important differences between the growth and loss of biomass in the period between 1986 and 2003. In the study area, 40% of areas that traditionally supported row crops and pasture hay were replaced my forestry plantations and herbaceous successional vegetation. This replacement has impacted the Chilean people and their agricultural way of life. The results obtained depict the usefulness of Change Vector Analysis (CVA) as a technique to analyze changes in biomass over time.

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Yes / This paper represents a significant advance on the issue of testing for implementations specified by P systems with transformation and communicating rules. Using the X-machine framework and the concept of cover automaton, it devises a testing approach for such systems, that, under well defined conditions, it ensures that the implementation conforms to the specification. It also investigates the issue of identifiability for P systems, that is an essential prerequisite for testing implementations based on such specifications and establishes a fundamental set of properties for identifiable P systems. / Marian Gheorghe and Savas Konur acknowledge the support from EPSRC (EP/I031812/1). Marian Gheorghe’s and Florentin Ipate’s work is partially supported by CNCS-UEFISCDI (PN-II-ID-PCE-2011-3-0688).

In order to ensure clean drinking water for all, it is crucial to understand potential upland stressors that compromise the quality of source waters treated by local community water systems (CWSs). Contamination associated with specific types of land cover can result in downstream water quality degradation, which may reduce the effectiveness of treatment by CWSs. Surface mining has been hypothesized as a source of drinking water degradation within the Central Appalachian region, which may result in adverse exposures and health disparities. The purpose of this study was to identify potential correlations between land cover and adverse birth outcomes (ABOs) through the application of watershed epidemiology, an emerging environmental health paradigm. Birth records for the Central Appalachian region were acquired from their respective state health departments from 2001 to 2015: each record contained the mother's street address, outcome variables, and covariates. Records were included in later analyses if they fell within an approximated CWS service area. Contributing land cover to each CWS was determined via previously delineated watersheds that relied on CWS intake points. A binomial generalized linear model was used to compare low birth weight (LBW), term low birth rate (tLBW), and preterm birth (PTB) incidence to CWS source watershed land cover, Safe Drinking Water Act (SDWA) violations, CWS size, and covariates related to the birth records. Source watershed mining and SDWA health based (HB) violations were significantly associated with greater risks for preterm birth (PTB) and low birth weight (LBW). Future work should be conducted to explore upstream flow impacts, address missing data in the birth records, and to more accurately represent CWS service areas to better characterize exposure. / Master of Science / Millions of individuals throughout the world are sickened by waterborne exposures every year. To ensure clean drinking water long-term, it is crucial to understand how human land cover might change the water quality of source watersheds, as this may impact the effectiveness of water treatment and increase adverse human health exposures. The goal of this effort is to understand whether land cover is linked to downstream adverse birth outcomes (ABOs) in Central Appalachia, a region of the United States previously associated with high disease incidence suspected to be partially linked to environmental exposure. Birth records were acquired for the years of 2001 to 2015 from four (VA, WV, TN, KY) respective state health departments. Each record contained the mother's address, outcome variables, and covariates (e.g., race, ethnicity). Births were located within approximate service areas for 140 surface water dependent community water systems (CWS) within the region. Data from each CWS, including weighted land cover proportions for their source watershed, were merged with the birth records according to approximate service areas. Statistical analysis suggested that higher source watershed levels of mining and urban development were associated with higher risks of preterm birth (PTB) and low birth weight (LBW). The number of health based (HB) violations associated with each CWS was also associated with both of these outcomes. Major limitations of this work include birth record data gaps and the lack of publicly available CWS service areas and/or water consumption rates, which does increase the risk of exposure misclassification.

Crop production, soil system, water quality, and economic impacts of four corn silage production systems were compared through a field study including 16 plots (4 replications of each treatment). Systems included a rye cover crop and application of liquid dairy manure in the spring and fall. The four management systems were: 1) traditional, 2) double-crop, 3) roll-down, and 4) undercut. In the fourth system, manure was applied below the soil surface during the undercutting process. In all other systems, manure was surface-applied. In the third system, the rye crop was flattened with a heavy roller after manure application. Simulated rainfall was applied within 48 h of manure application. Measured constituent concentrations in runoff were compared with water quality criteria. Costs and returns of all systems were compared. The undercut system reduced loadings of all nutrients, but increased total suspended solids (TSS) concentration as compared with all other systems. The mean volume of runoff from the undercut system was less than half that from any other system, which influenced all constituent loadings. Mean TSS concentration in runoff from the undercut system was over three times the mean of any other system. The roll-down system had no significant effect on water quality as compared to the traditional system. The undercut system was reasonably effective in keeping phosphate phosphorus levels below the criterion set for bathing water. None of the systems generally exceeded nitrate nitrogen concentration criteria. However, total phosphorus, orthophosphate, fecal coliform and e. coli criteria for drinking, bathing, shellfish harvest, and aesthetics were regularly exceeded by all of the systems. There were no differences among the treatments in effects on bacterial concentrations. The double-crop system produced significantly higher net returns than all other systems only if the value of the rye crop was $92.31/Mg or more. There were no significant differences in net returns of the traditional, roll-down, or undercut systems. / Ph. D.

I investigated the relationships between historical and contemporary land cover and macroinvertebrate communities, water quality, and nutrient levels in 10 streams in a historically agricultural region of the Virginia Piedmont. Historical (1963) and contemporary (2011) impervious surface, open area, and forested cover were evaluated using aerial photos and GIS data. Macroinvertebrates were collected in the fall of 2012 and spring of 2013. Water quality parameters (temperature, conductivity, alkalinity, hardness, and DO) and nutrient concentrations (NH3+NH4, PO4-P, NO3-N, Cl, and SO4) were measured at each site. Overall, forest cover decreased by 6.29%, open area decreased by 1.46%, and impervious surface increased by 4.83% from 1963 to 2011. Macroinvertebrate communities were explored using Principal Coordinates Analysis and were found to be significantly related to 2011 percent impervious surface. Water quality parameters were not significantly related to contemporary or historical land cover. Nitrate was negatively related with 2011 forest cover and positively related with 2011 open area; chloride was positively related with 2011 impervious surface and negatively related with 2011 open area. For the 10 watersheds included in this study, contemporary land cover is a better predictor of macroinvertebrate assemblages and nutrient concentrations than historical land cover. / Master of Science

While cover crops are actively growing, they compete with winter annual weeds. Studies were conducted to determine the ability of early planted cover crop monocultures and mixtures and a fall applied residual herbicide to compete with winter annual weeds. Cereal rye containing cover crops provided the greatest control of winter weeds in May. Flumioxazin, as a fall applied herbicide, controlled winter weeds in December, but control did not persist until May. Once cover crops are terminated, their residue suppresses summer annual weeds. A greenhouse experiment studying the effects of cereal rye biomass on common ragweed and Palmer amaranth control and light penetration and two field experiments to determine the effects of cereal rye and wheat cover crop biomass terminated with a roller crimper or left standing on summer weed control and light penetration were conducted. For summer weed control, as cover crop biomass increased, weed control increased, light penetration decreased, soil temperature decreased, and soil moisture increased. Standing cover crop residue provided greater control of common ragweed compared to rolled residue until 8400 kg ha⁻¹ of cover crop biomass. As cover crop biomass increased, rolled cover crop residue reduced light penetration compared to standing residue. Wheat cover crop residue increased soil moisture more compared to cereal rye residue. Cover crops must become established to produce adequate biomass to compete with weeds. Herbicide carryover has the potential to reduce cover crop establishment. A study was conducted to evaluate the potential for 30 different residual herbicides applied in the cash crop growing season to carryover to 10 different cover crops. While visible injury was observed, cover crop biomass was similar to the nontreated check in all cases, indicating that herbicide carryover to cover crops is of little concern. Herbicide carryover has few biological effects on establishment of cover crops, under the conditions and herbicides evaluated, that are effective at competing with winter annual weeds and suppressing summer annual weeds. / Master of Science in Life Sciences / Cover crops are grown after the cash crop has been harvested and terminated before another is planted. They are grown for environmental and agronomic benefits and not harvested. Cover crops improve soil health, reduce erosion, prevent nutrient loss, and control weeds. Cover crops can compete with weeds while they are actively growing. Their residue can create a mulch layer to reduce weed establishment and limit the amount of light reaching weed seed to reduce germination and establishment. As winter cover crops are growing, they compete with winter weeds for sunlight, nutrients, and water. Fall applied herbicides that remain active in the soil are also utilized to control winter weeds in between cash crop growing seasons. Experiments evaluated the ability of cover crop monocultures and cover crop mixtures compared to a fall applied herbicide to compete with winter annual weeds. Monocultures and mixtures of cereal rye, crimson clover, hairy vetch, and forage radish were utilized. Cereal rye containing treatments provided the greatest control of winter weeds in the spring. The fall applied herbicide provided adequate control of winter weeds in December, but control did not persist until the May, indicating that a fall applied herbicide will not provide control of winter weeds from cash crop harvest to the next cash crop planting. As cover crop biomass increases, summer annual weed control increases. Cover crops are usually terminated with herbicide and left standing in Virginia, but the use of a roller crimper lays down residue and creates a mulch layer. Experiments compared the effects of cereal rye and wheat cover crops at different biomass rates terminated with herbicide only (left standing) or a roller crimper and herbicide on summer weed control, light penetrating the cover crop canopy and reaching the soil surface, soil moisture, and soil temperature. As cover crop biomass increased, weed control increased, light reaching the soil surface decreased, soil temperature decreased, and soil moisture increased. Standing cover crop residue provided greater weed control until 8400 kg ha⁻¹ of cover crop biomass was reached. After 8400 kg ha⁻¹ rolled cover crop residue provided greater control, but control from standing and rolled were both greater than 80% compared to the no cover control. Cereal rye intercepted more light than wheat cover crop residue at less than 6000 kg ha⁻¹ of cover crop biomass was achieved. Rolled cover crop residue intercepted more light than standing residue. Established cover crops most produce adequate biomass to effectively control weeds. Herbicides applied during the cash crop growing season to control weeds can remain active in the soil and reduce the establishment of subsequently planted cover crops. Experiments evaluated the potential for different herbicides applied during the cash crop, such as corn, cotton, or soybeans, to remain in the soil at high enough concentrations to injure cover crops commonly utilized in the Mid-Atlantic region. Cover crops utilized were wheat, barley, cereal rye, oats, annual ryegrass, forage radish, Austrian winter pea, crimson clover, hairy vetch, and rapeseed. Results suggest that little potential exists for the herbicides utilized to persist in the soil to injure the five grass cover crop species utilized. There is the potential for some herbicides to injure forage radish, crimson clover, and rapeseed, but no reduction in cover crop biomass was observed, indicating there is little concern for herbicide carryover to cover crops. Residual herbicide carryover has little effect on establishment of cover crops and does not reduce cover crop biomass, under the conditions and herbicides tested in this study. Cover crops can effectively compete with winter weeds, and as cover crop biomass increases, summer annual weed control increases.

Cover crops have become an important part of cropping systems in the United States, especially in the Mid-Atlantic region. Rapeseed is a popular choice due to its deep growing taproot which creates soil macropores and increases water infiltration. If not properly terminated rapeseed can become problematic due to its pod-shattering tendency and its difficulty to terminate with herbicides once it enters reproductive growth. Results indicate termination of rapeseed is most effective when the cover crop is small. Combinations that successfully terminated rapeseed include glyphosate plus 2,4-D and paraquat plus 2,4-D. Halauxifen-methyl is a new Group 4 herbicide marketed for preplant burndown horseweed (Conyza canadensis L.) control. Previous research indicates that halauxifen effectively controls glyphosate-resistant horseweed. However, little is known about control of other common winter annual weeds by halauxifen. Results indicate halauxifen has a narrow spectrum of control providing adequate control (>80%) of horseweed, henbit (Lamium amplexicaule L.), and purple deadnettle (Lamium purpureum L.), while failing to control cutleaf evening-primrose (Oenothera laciniata Hill), curly dock (Rumex crispus L.), purple cudweed (Gamochaeta purpurea L. Cabrera), common chickweed (Stellaria media L.), and mousear chickweed (Cerastium L.). Little is known of cotton (Gossypium hirsutum L.) tolerance to halauxifen applied preplant burndown. Results indicate cotton is more tolerant to halauxifen than 2,4-D or dicamba when the interval between preplant application and cotton planting is less than 30 days. / Master of Science in Life Sciences / Cover crops are an important part of cropping systems in the United States, especially in the Mid-Atlantic region. Producers utilize cover crops to aid in weed suppression, reduce soil erosion, as well as to increase soil health. Cereals, legumes, and Brassicaceae species are popular cover crops planted either as monocultures or mixtures. Rapeseed can become problematic due to its difficulty to terminate once it enters reproductive stage, as well as its podshattering characteristic. Experiments were conducted to evaluate various herbicides and herbicide combinations for rapeseed termination two application timings. At three locations where rapeseed averaged 12 cm in height at early termination, and 52 cm in height at late termination, glyphosate + 2,4-D was most effective, controlling rapeseed (96%) 28 days after early termination (DAET). Paraquat + atrazine + atrazine (92%), glyphosate + saflufenacil (91%), glyphosate + dicamba (91%), and glyphosate (86%) all provided at least 80% control 28 DAET. Paraquat + 2,4-D (85%), glyphosate + 2,4-D (82%), and paraquat + atrazine + mesotrione (81%) were the only treatments to provide at least 80% control 28 days after late termination (DALT). At one location where rapeseed was much taller (41 cm early termination; 107 cm late termination), herbicides were much less effective, as no herbicide treatments provided greater than 80% control. Results indicated that rapeseed size at time of termination was more critical to successful termination than herbicide choice. Prior to the development of glyphosate-resistant horseweed, producers were able to control horseweed and other weeds with glyphosate applied preplant burndown. Producers now rely on auxin herbicides tank mixed with glyphosate and a residual herbicide to control horseweed and other winter weeds prior to cash crop planting. Experiments were conducted to evaluate halauxifen-methyl, a new Group 4 herbicide, for control of horseweed and other commonly encountered winter annual weeds. Halauxifen (89%) controlled small horseweed (<5 cm in height at time of application) similar to dicamba (91%), while providing better control of large horseweed (79%) (>15 cm in height at time of application) than either dicamba (77%) or 2,4-D evaluated (64%). Halauxifen provided adequate control (>80%) of henbit (Lamium amplexicaule L). and purple deadnettle (Lamium purpureum L.), while failing to effectively control of cutleaf evening-primrose (Oenothera laciniata Hill), curly dock (Rumex crispus L.), purple cudweed (Gamochaeta purpurea L. Cabrera), common chickweed (Stellaria media L. Vill.), and mousear chickweed (Cerastium L.). Results indicate that halauxifen has a narrow spectrum of control and should be tank mixed with 2,4-D or glyphosate in order to control weeds other than horseweed and henbit. Glyphosate plus dicamba or 2,4-D plus a residual herbicide is typically applied prior to cotton planting. Previous research has shown that as long as rainfall requirements and rotation intervals are met, no adverse effects on cotton is observed from 2,4-D or dicamba herbicides. Little is known of cotton tolerance to halauxifen applied preplant burndown. Experiments were conducted to determine if halauxifen applied sooner than the labeled 30-day rotation interval would injure cotton. Very little injury was observed from halauxifen (9%) applied at-planting, however dicamba (26%) and 2,4-D (21%) applied at the same timing did injure cotton. Auxin herbicides applied earlier in the season resulted in little injury (<2%). Early season injury was transient as cotton recovered later in the season and seedcotton yield was unaffected.