The purpose of this study was to examine the direct and indirect effects of ultraviolet-B (UV-B) radiation on freshwater protists. Laboratory experiments were conducted in order to determine the importance of photoenzymatic repair (PER) of UVBinduced DNA damage in the heterotrophic nanoflagellate Paraphysomonas sp. Investigations into the combined effects of UV-B and chromophoric dissolved organic matter (CDOM) were conducted in laboratory experiments in which protist cultures were exposed to UV-B radiation in the presence and absence of water amended to a higher CDOM concentration in order to determine the ability of CDOM to act as a UV-B filter and as a potential nutrient source. Field experiments examined the responses of natural communities of protists and bacteria to ultraviolet radiation (UVR) in the presence of high and low concentrations of CDOM. Ultraviolet-B radiation (UV-B; 280 – 320 nm) negatively affects many aquatic organisms, including heterotrophic flagellates, by directly damaging DNA. The quantity of UV-B reaching the surface of a lake varies with atmospheric chemistry, including stratospheric ozone, and the presence of large holes (Alldredge 1977) in this ozone shield during the last decade resulted in historically high UV-B levels. In aquatic systems, the nature of the damage to organisms depends on the intensity and duration of solar radiation, plus its attenuation in the water column. The amount of UV-B damage is highly dependent on the concentration of CDOM in the water column because CDOM strongly absorbs UV-B radiation. This protective role of CDOM is likely to be reduced in areas where warmer, drier climate decreases watershed runoff, which ultimately results in acidification and increased CDOM photodegradation. However, CDOM also may also act as an organic carbon source for bacteria and stimulate growth of the microbial food web, including bacterivores like heterotrophic flagellates. The effect of UV and CDOM interactions on the microbial food web is not well understood, but climate-related increase in CDOM in an oligotrophic lake could increase the heterotrophic microbial food web impact by reducing UV-B damage and increasing available resources. Since aquatic organisms, including protists, are differentially susceptible to UV-B radiation, climate change effects on CDOM and UV-B attenuation are likely to alter the ecology and community structure of aquatic systems. This thesis describes investigations into the direct and indirect effects of UV-B radiation on freshwater protozoa. The role of PER of direct UV-B induced DNA damage was examined in laboratory experiments that compared the survival and population growth of the heterotrophic nanoflagellate Paraphysomonas sp. at two environmentally relevant temperatures. The results from these experiments demonstrated the reliance of Paraphysomonas sp. on PER, with 100% mortality in the absence of the photorepair radiation that activates photoenzymatic repair enzymes. The ability of Paraphysomonas sp. to recover from exposure to UV-B radiation declined in flagellates adapted to 15°C relative to the same exposures at 20°C. Experiments examining the direct and indirect effects of UV-B radiation and CDOM on freshwater protists conducted in the laboratory and in an oligotrophic lake in the Pocono Mountains showed that potential DNA damage resulting from UV exposure is reduced and microbial growth may be enhanced with an increase in CDOM concentration. / Biology

Yes / This article considers how cuts in Government funding will affect continuing professional development and mentorship training for NHS staff

Made available in DSpace on 2014-12-17T14:56:19Z (GMT). No. of bitstreams: 1 ArthurDLL_DISSERT.pdf: 2517302 bytes, checksum: c3d693c770dc1c58bad5f378aba6d268 (MD5) Previous issue date: 2014-06-28 / The increasing demand for high performance wireless communication systems has shown the inefficiency of the current model of fixed allocation of the radio spectrum. In this context, cognitive radio appears as a more efficient alternative, by providing opportunistic spectrum access, with the maximum bandwidth possible. To ensure these requirements, it is necessary that the transmitter identify opportunities for transmission and the receiver recognizes the parameters defined for the communication signal. The techniques that use cyclostationary analysis can be applied to problems in either spectrum sensing and modulation classification, even in low signal-to-noise ratio (SNR) environments. However, despite the robustness, one of the main disadvantages of cyclostationarity is the high computational cost for calculating its functions. This work proposes efficient architectures for obtaining cyclostationary features to be employed in either spectrum sensing and automatic modulation classification (AMC). In the context of spectrum sensing, a parallelized algorithm for extracting cyclostationary features of communication signals is presented. The performance of this features extractor parallelization is evaluated by speedup and parallel eficiency metrics. The architecture for spectrum sensing is analyzed for several configuration of false alarm probability, SNR levels and observation time for BPSK and QPSK modulations. In the context of AMC, the reduced alpha-profile is proposed as as a cyclostationary signature calculated for a reduced cyclic frequencies set. This signature is validated by a modulation classification architecture based on pattern matching. The architecture for AMC is investigated for correct classification rates of AM, BPSK, QPSK, MSK and FSK modulations, considering several scenarios of observation length and SNR levels. The numerical results of performance obtained in this work show the eficiency of the proposed architectures / O aumento da demanda por sistemas de comunica??o sem fio de alto desempenho tem evidenciado a inefici?ncia do atual modelo de aloca??o fixa do espectro de r?dio. Nesse contexto, o r?dio cognitivo surge como uma alternativa mais eficiente, ao proporcionar o acesso oportunista ao espectro, com a maior largura de banda poss?vel. Para garantir esses requisitos, ? necess?rio que o transmissor identifique as oportunidades de transmiss?o e que o receptor reconhe?a os par?metros definidos para o sinal de comunica??o. As t?cnicas que utilizam a an?lise cicloestacion?ria podem ser aplicadas tanto em problemas de sensoriamento espectral, quanto na classifica??o de modula??es, mesmo em ambientes de baixa rela??o sinal-ru?do (SNR). Entretanto, apesar da robustez, uma das principais desvantagens da cicloestacionariedade est? no elevado custo computacional para o c?lculo das suas fun??es. Este trabalho prop?e arquiteturas eficientes de obten??o de caracter?sticas cicloestacion?rias para serem empregadas no sensoriamento espectral e na classifica??o autom?tica de modula??es (AMC). No contexto do sensoriamento espectral, um algoritmo paralelizado para extrair as caracter?sticas cicloestacion?rias de sinais de comunica??o ? apresentado. O desempenho da paraleliza??o desse extrator de caracter?sticas ? avaliado atrav?s das m?tricas de speedup e efici?ncia paralela. A arquitetura de sensoriamento espectral ? analisada para diversas configura??es de probabilidades de falso alarme, n?veis de SNR e tempo de observa??o das modula??es BPSK e QPSK. No contexto da AMC, o perfil-alfa reduzido ? proposto como uma assinatura cicloestacion?ria calculada para um conjunto reduzido de frequ?ncia c?clicas. Essa assinatura ? validada por meio de uma arquitetura de classifica??o baseada no casamento de padr?es. A arquitetura para AMC ? investigada para as taxas de acerto obtidas para as modula??es AM, BPSK, QPSK, MSK e FSK, considerando diversos cen?rios de tempo de observa??o e n?veis de SNR. Os resultados num?ricos de desempenho obtidos neste trabalho demonstram a efici?ncia das arquiteturas propostas

Les rayons UV du soleil causent une variété de dommages dans l'ADN, parmi lesquels les dimères cyclobutilyques de pyrimidines (CPD) sont considérés comme hautement toxiques et dommageables pour un organisme. Par conséquent, il est important de comprendre comment la machinerie de réparation par excision des nucléotides (la NER), assure la réparation in vivo des CPD présents dans l'ADN empaqueté sous forme de chromatine. Il est connu que la présence du nucléosome inhiberait la NER, mais les détails fonctionnels demeurent mal compris, de même que les mécanismes cellulaires nécessaires pour contourner cette inhibition offerte par la chromatine. Chez la levure Saccharomyces cerevisiae, les gènes SIR (SIR1, SIR2, SIR3 et SIR4 ) permettent la formation d'une structure hétérochromatique sur le locus du type sexuel et les télomères. Cependant, l'impact de cette hétérochromatine sur la réparation des CPD est très peu étudié.Les travaux présentés dans cette thèse de doctorat ont permis de caractériser l'impact des gènes SIR dans la survie des cellules après irradiation aux rayons UV, de même que dans la réparation de l'ADN des régions hétérochromatiques. Premièrement, à l'aide d'une méthode basée sur le suivi de la croissance en milieu de culture liquide, nous avons démontré que les mutants sir[delta] sont plus résistants aux rayons UV par rapport aux cellules de types sauvages. Ce phénotype serait relié à l'effet de pseudo-diploïdie présent dans ces mutants, et plus précisément à la recombinaison homologue en phase G2/M du cycle cellulaire.Les protéines Sir ne joueraient donc pas un rôle directement dans la réparation des CPD. Par la suite, nous avons procédé à l'analyse de la cinétique de réparation de l'ADN du locus du type sexuel et des télomères dans des cellules de type sauvage et des mutants si2r[delta], sir3[delta], et rad26[delta] . À partir des résultats obtenus, nous avons pu tirer différentes conclusions préliminaires laissant croire que la présence de l'hétérochromatine faite par les protéines Sir n'inhiberait pas davantage la réparation par rapport à la présence des nucléosomes, du moins dans les régions sous-télomériques. De plus, nos résultats démontreraient que la réparation couplée à la transcription pourrait jouer un rôle important dans la réparation de ces régions. Ces hypothèses devront évidemment être testées.

This phenomenographic study, explores the collaborative open learning experience of academic staff and open learners in cross-institutional academic development settings, and adds to what is known in these settings. It provides new insights for academic developers and course designers about the benefits of crossing boundaries (i.e. open learning) in an academic development context and proposes an alternative model to traditional academic Continuing Professional Development (CPD). It engages academic staff in experiencing novel approaches to learning and teaching and developing as practitioners through engagement in academic CPD that stretches beyond institutional boundaries, characterised by diversity and based on collaboration and openness. Data collection was conducted using a collective case study approach to gain insights into the collective lived collaborative open learning experience in two authentic cross-institutional academic development settings with collaborative learning features designed in. At least one of the institutions involved in each course was based in the United Kingdom. Twenty two individual phenomenographic interviews were conducted and coded. The findings illustrate that collaborative open learning was experienced as two dynamic immersive and selective patterns. Boundary crossing as captured in the categories of description and their qualitatively different variations, shaped that experience and related to modes of participation; time, place and space; culture and language as well as diverse professional contexts. Facilitator support and the elasticity of the design also positively shaped this experience. The community aspect influenced study participants' experience at individual and course level and illuminated new opportunities for academic development practice based on cross-boundary community-led approaches. The findings synthesised in the phenomenographic outcome space, depicting the logical relationships of the eleven categories of description in this study, organised in structural factors, illustrate how these contributed and shaped the lived experience, together with a critical discussion of these with the literature, aided the creation of the openly licensed cross-boundary collaborative open learning framework for cross-institutional academic development, the final output of this study. A design tool developed from the results is included that aims to inform academic developers and other course designers who may be considering and planning to model and implement such approaches in their own practice.

This thesis addresses the fabrication, evaluation, and analysis of the probe tip of a non-vibrating contact potential difference sensor. The non-vibrating contact potential difference (nvCPD) probe measures the work function variation on a conducting surface and recent experiments performed to measure the size of surface features have shown poor correlation between actual and calculated probe tip dimensions. In order to address this deficiency, experiments were done and an analytical model was developed, including fringe electrical fields, that predicts the shape of the nvCPD probe signal as a function of probe tip geometry, work function variation, and experimental parameters. Probe tips were constructed with varying geometric properties and experiments using these probe tips were compared to a model. There was good correlation of the nvCPD probe output for a known work function change and probe tip geometry. The effective area of the probe tip resulting from electrical field fringing is expected to increase with dielectric thickness to a finite value, based on pre-existing electrostatic models for a shielded parallel plate capacitor. The minimum fringe field obtained in these experiments was for a 3.18mm diameter probe tip with a dielectric thickness of 0.20mm. The fringe field diameter was 3.38mm at a fly height of 0.60mm, representing an effective probe tip area increase of 13%.

No description available.

This thesis explores the Continuing Professional Development (CPD) of mid-career artists in England and the South West of England in particular during 2000 to 2010. It identifies what their needs are and asks what CPD means to them; how they navigate their careers through their practice; how they articulate their needs; and how they negotiate to fulfil those needs. It examines to what extent the providers’ thinking about, and provision of, CPD in the region is aligned with the needs of the artists themselves. The individual narratives of artists are represented at the centre of this research. The research was developed in collaboration with University of Plymouth and the CPD agency, ArtsMatrix Ltd. Research methods used to collect data included extended dialogues with the artists Alyson Hallett, Mariele Neudecker, Helen Poynor and Phil Smith, via a series of walking interviews, using walking as an ethnographic research tool. By walking I engaged with a literal momentum of movement paralleling the physical and theoretical momentums of the artists’ practices. Policy and literature reviews; group interviews; artist interviews; desk-based research; observation and attendance at artist-led seminars and practice groups were also used. The research contrasts two CPD Schemes: The Contemporary Craft Fellowship Scheme, and The Artist as Cultural Agent: DIY. The thesis includes a policy and provision review of CPD literature in the UK and South West of England over the past ten years; a mapping of South West CPD provision for artists; and the identification and application of relevant theoretical and critical approaches to place, space, language and momentum in order to consider CPD provision in relation to the articulation, situation and concept of a career. This thesis argues that the language of CPD can constrain as much as enable artists’ development; that the terms “mid-career” and “South West” are open to contestation and can affect provider conceptualisations of artists in the region; and that artists need professional development throughout their careers but may not name it as such. I advocate for policy and provision to understand artists’ need to be supported “throughout” their practices rather than at certain points in a career, and advocate walking as both a research method and as one of a number of facilitative practices for those who provide CPD with or for experienced artists. I also advocate for artist-led CPD initiatives and an administrative support agency for artists.

La réponse cellulaire aux ultra-violets (UV), ou réponse UV, est une réponse complexe et spécialisée dans l’adaptation et la tolérance des dommages aux UV. Celle-ci est initiée par un grand nombre d’évènements moléculaires et de signalisation nucléaire mais aussi au niveau de la membrane plasmique ou du cytoplasme. L’importance et l’influence exactes de ces évènements sur la réparation par excision de nucléotides (NER) des dommages UV à l’ADN sont encore mal comprises et doivent encore être méthodiquement démontrées. Dans cette thèse, grâce à l’utilisation d’une méthode sensible d’analyse de la réparation NER basée sur la cytométrie en flux, il est montré, dans un premier temps, que l’activité des voies MAPK (Mitogen-Activated Protein Kinases), qui sont des voies de signalisation de stress UV d’origine cytoplsamique, ne participent pas à l’efficacité de réparation NER des dommages UV dans les cellules humaines. En effet, l’abrogation de la signalisation MAPK, par inhibition pharmacologique, par utilisation de mutants dominant-négatifs ou par inhibition de leur expression endogène, ne révèlent aucun changement de la cinétique de réparation des dommages UV par excision de nucléotides. Cependant, l’utilisation de cette même méthode de réparation, mais cette fois, appliquée pour l’étude de réparation NER en fonction du cycle cellulaire, a permis de mettre en évidence la nécessité fonctionnelle de l’ADN polymérase translésionnelle eta (Pol η) dans la réparation NER des dommages UV, uniquement en phase S. Cette observation fut initialement caractérisée dans les cellules de patients affectés du syndrome variant de xérodermie pigmentaire (XP-V) puis, confirmée ensuite par l’inhibition de l’expression de Pol η endogène ou par la complémentation avec des mutants non-fonctionnels dans les cellules XP-V. Ces résultats indiquent que, contrairement à la réponse UV MAPK cytoplasmique, les évènements nucléaires comme la synthèse translésionnelle, peuvent influencer l’efficacité de réparation NER en phase S. Plus particulièrement, ces données établissent un lien possible entre la réparation NER en phase S et les niveaux de stress réplicatifs, révélé ici par la déficience fonctionnelle Pol η ou ATR. Les observations, présentées dans cette thèse, renforcent un rôle du point de contrôle S aux UV sur l’efficacité de la réparation NER et suggèrent que l’inhibition NER, observée en phase S dans les cellules XP-V, est modulée par le stress réplicatif. Un tel moyen de contrôle pourrait avoir une action plutôt protectrice pendant cette phase critique du cycle cellulaire. Mots clés: UV, translésionnelle, eta, MAPK, NER, CPD, cytométrie, phase-S, tolérance. / The UV-response is a complex cellular response to UV irradiation, which allows cellular adaptation and protection against deleterious effects of UV. This specialized response involves numerous molecular and signaling events from plasma membrane and from the nucleus represented, among others, by Mitogen-Activated Protein Kinase (MAPK) pathway activation and translesion synthesis respectively. More particularly, the exact role of these events on the removal UV-induced DNA damage by nucleotide excision repair (NER) in human cells is poorly understood and documented. By using a sensitive flow cytometry based-NER assay, presented and validated in this thesis, to quantify the removal of UV-DNA damage, it was unexpectedly found that Mitogen-Activated Protein Kinase (MAPK) signalling, originating from the the plasma membrane, does not regulate the efficiency of UV-induced DNA damage repair in human cells. Indeed, MAPK inhibition with pharmacological inhibitors, expression of short-hairpin RNA or dominant negative mutant, all together, substantiate fully the lack of effect of this signalling pathway on UV-damage removal by NER in human primaries and tumorous cells. Surprisingly, the same NER assay, applied to quantify the removal of UV-induced DNA damages as a function of the cell cycle, has shown a requirement of functional translesion synthesis polymerase eta (Pol η) for efficient UV-DNA damage repair in human cells uniquely during S-phase, where its function is required for the bypass of UV DNA damage. This observation, originally made in fibroblasts from xeroderma pigmentosum variant syndrome (XP-V) afflicted patients, was further confirmed in normal human cells, by abrogation of endogenous Pol η expression or by complementation with Pol η in XP-V cells. All together, the data presented here, indicate that MAPK signaling play no role in NER-mediated UV-damage removal, but highlight a role for UV-DNA damage tolerance response, as translesion synthesis, in regulation of NER efficiency. More particularly, these observations establish a potential link between the S-Phase Repair (SPR) of UV-DNA damages and replicative stress, revealed by a deficiency of Pol η or ATR. This SPR defects seen under acute replicative stress conditions could impact tumorogenesis or chemotherapy outcomes. Moreover, SPR defects, seen in XP-V cells could be controlled by replicative stress, and also reflect a protective coordination to reduce high risks of genetic or chromosomal aberrations that may occur during DNA replication upon UV exposure. Key Words: Translesion, UV, TLS, eta, MAPK, NER, S-phase, flow-cytometry, CPD.

This qualitative study focused on four secondary school physics teachers in Bangladesh using semi-structured interviews and observations to explore their understanding about the relationship between practical work and developing students’ conceptual knowledge of physics. Recent studies indicate that practical work helps secondary science students easily and effectively learn the concepts and theories of physics. However, the secondary school physics teachers in Bangladesh in this study did not provide students with practical work during classroom teaching. Rather, they provided practical work in separate practical classes. Although the teachers believed that practical work made their teaching and also students’ learning easier and effective, they did not offer frequent practical demonstrations in teaching the contents of physics. The major findings of the study include that teachers used mostly transmissive pedagogy to assist students to understand physics concepts and theories. Even though there are clear and specific instructions for the teachers to do demonstrations in the secondary physics curriculum, there were constraints on teachers and on students trying to conduct practical work. Constraints included: a lack of sufficient equipment. Teachers and students in non-government schools faced comparatively more difficulties than those in government schools. Low teacher/student ratios and no positions for laboratory assistants were reasons given for teachers’ intense workloads. This study implies a need to provide government and non-government schools with necessary equipment for doing practical work; to appoint sufficient teachers with higher studies and training that includes practical work in physics; to create positions for laboratory assistants; to set up classrooms with a smaller number of students; and to develop awareness of the value of practical work among school administration and among physics teachers.