To mitigate the problems of depleting and soaring price of fossil fuels, the production and use of renewable energy have been vigorously promoted. In Europe, the role of biologically-derived fuels and in particular biodiesel is gradually increasing in prominent. Rapeseed biodiesel is the most widely produced in Europe. As a consequence, enormous amount of by-products from production processes are being generated. Current strategies for managing these by-products (mainly rapeseed meal and crude glycerol) seem not to be economically sustainable. More efficient utilisation could add more value to the production chain which in turn would raise the competitiveness of biodiesel compared to petro-diesel. The aim of the project reported in this thesis was to study the feasibility of producing a value added product, polyhydroxybutyrate (PHB), from by-products generated from rapeseed biodiesel production processes as well as to investigate the effects of methanol, a major impurity in crude glycerol, on growth of Cupriavidus necator, a PHB-producing micro-organism.The preliminary study of C. necator growth in crude glycerol based media revealed that optimum concentration of crude glycerol was in a range 15-25 g/L. It was also found that slight changes in the carbon to nitrogen ratio of the feedstock did not significantly affect the growth while methanol at concentrations beyond 10 g/L did. A model based on a saturation equation was developed and used to successfully predict the inhibition of growth by methanol. From the developed model, mechanisms of the inhibition were proposed. The model could also be used to predict satisfactorily growth or productivity rates in other systems containing short-chain alcohols. The growth in solutions derived from rapeseed meal (designated as hydrolysate) via solid-state fermentation by Aspergillus oryzae followed by hydrolysis of the fermented solids was also studied. The biomass production was found to increase as a function of initial free amino nitrogen (FAN) concentration presented in the hydrolysate. However, at higher initial FAN concentrations, a lower conversion of nitrogen to biomass was observed. PHB production was studied using a feedstock which was a mixture of the hydrolysate and crude glycerol. Total biomass concentration reached 28.8 g/L at 120 h with 86% PHB content. PHB productivity and PHB yield on glycerol were 0.21 g/L•h and 0.32 g/g respectively. These results were comparable with those obtained when pure glycerol and synthetic crude glycerol were used, suggesting that, technically, the use of the generic rapeseed- and crude glycerol-based feedstock to produce PHB is feasible.Overall, the feasibility of producing PHB from rapeseed biodiesel by-products has been demonstrated. The satisfactory result leads to the more important outlook that the generic feedstock derived from rapeseed biodiesel by-products has the potential to be used to produce a wide range of products depending on the micro-organism used. Further development of this process to improve nutrient production efficiency as well as product yields and subsequent integration of the process into the biodiesel production process could well be an important contribution in the development of a sustainable biodiesel industry.

Leveduras oleaginosas possuem a capacidade de sintetizar grande quantidade de lipídeos em suas células, gerando produtos de alto valor agregado através de um processo sustentável. Um perfil de ácidos graxos poli-insaturados é de grande interesse por parte das industrias alimentícia e farmacêutica e têm sido alvo de diversos estudos em relação à sua produção e efeitos benéficos à saúde. O presente trabalho teve como objetivo produzir ácidos graxos poli-insaturados pela levedura Meyerozyma guilliermondii BI281 utilizando glicose, glicerol residual e permeado de soro de queijo como fontes de carbono. Avaliou-se o metabolismo da levedura em meios de cultivo com diferentes composições (“meio A” e “base YM”) e comparou-se o seu desempenho em razões C/N 100: 1 e 50:1. A levedura foi capaz de metabolizar todos os substratos testados e o meio de cultivo “A” com glicerol residual e razão C/N 50:1 mostrou-se o mais adequado, obtendo valores de 5,67 g.L-1 de biomassa e 1,04 g.L-1 de lipídeos, representando aproximadamente 18 % do peso da massa seca de biomassa. Esta condição foi escalonada para biorreator de 2 L, onde condições de aeração e controle de pH puderam ser avaliadas, apresentando quantidade semelhante de lipídeos (1,08 g.L-1) e uma maior produção de biomassa (7,05 g.L-1). Os ácidos graxos sintetizados apresentaram em sua composição ácido láurico (C12:0), ácido mistírico (C14:0), ácido palmítico (C16:0), ácido pamitoleico (C16:1), ácido esteárico (C18:0), ácido oleico (C18:1 n-9), ácido linoleico (C18:2 n-6) e ácido linolênico (C18:3 n-3). / Oleaginous yeasts are able to synthesize high amounts of lipids in their cells, producing high added-value products through a sustainable process. Food and pharmaceutical companies have great interest in polyunsaturated fatty acids depending on their profiles and many studies related to their production and health benefits have been carried out. The aim of this study was to produce polyunsaturated fatty acids by the yeast Meyerozyma guilliermondii BI281A using glucose, crude glycerol, and cheese whey permeate as carbon sources. Yeast metabolism was evaluated in different compositions of culture media (“A” and “YM”) and at different C/N ratios (100:1 and 50:1). The yeast was able to assimilate all substrates tested, and medium “A” with crude glycerol as carbon source at a C/N ratio of 50:1 had the most efficient result with biomass production of 5.67 g.L-1 and lipid production of 1.08 g.L-1, which represented 18 % of dry cell weight. This composition was scaled-up to a 2 L bioreactor, where it was possible to measure pH and aeration conditions, and showed similar lipid production (1.08 g.L-1) and higher biomass production (7.05 g.L-1). Fatty acids profile obtained was composed by lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid(C18:1), linoleic acid (C18:2 n-6), and linolenic acid (C18:3 n-3).

Anaerobic digestion can be used to sustainably treat the organic byproducts of the biodiesel process (crude glycerol and biodiesel wastewater) while generating a renewable natural gas to be used for heating or electricity generation. The purpose of this thesis was to (1) investigate the possibility of co-digestion of biodiesel byproducts without use of external substrates or pretreatment and (2) assess the impact of various acclimatization rates on the stability and efficiency of such a system. Two inocula (effluent from a wastewater treatment plant digester and from an agro-industrial waste digester) and two acclimatization rates were studied. The results showed that co-digestion of crude glycerol and biodiesel wastewater at high organic loading rates(up to 6.8 g COD L-1day-1)is possible without addition of other substrates or pretreatment.Thecumulative biogas production of the digesters using inoculum from the agro-industrial waste digester was statistically greater than the digesters using the wastewater treatment plant digester, indicating that similar inoculum could be useful for additional experiments.In addition,maximum efficiency due to a slower rate of acclimatization was higher for both inocula, up to a maximum average daily biogas yield of 621 mL biogas g-1COD added.Finally, comparison of two methods for measuring gas production (mass difference and volumetrically using a syringe) revealed a reasonable correlation(R2= 0.97)between the methods. Additional validation could lead to use of the mass difference method as a validation method or an alternative gas production measurement method.

No description available.

A utilização de combustíveis renováveis tem se destacado nos últimos anos, principalmente devido aos impactos ambientais gerados pelo uso de combustíveis fósseis e escassez dos mesmos. Dentre os combustíveis renováveis destaca-se o biodiesel, cujo processo produtivo apresenta como principal subproduto o glicerol bruto, que tem instigado nos últimos anos intensas discussões sobre a problemática de sua destinação e disposição final. Apesar de ser utilizado na indústria química, o seu teor de impurezas limita o seu processamento industrial. Buscando agregar valor a esse resíduo bruto e contribuir para a produção de bioenergia, este trabalho teve por intuito avaliar a produção dos biocombustíveis hidrogênio e metano, assim como de intermediários químicos a partir de glicerol bruto como substrato em reatores anaeróbios de leito fluidificado (RALF), mesofílicos (30°C), utilizando inóculo de cultura mista. O RALF-H2, com o objetivo de produzir hidrogênio e metabólitos solúveis, foi submetido à concentração de 10 g.L-1 de glicerol e variação do tempo de detenção hidráulica (TDH) de 8, 6, 4, 2, 1 e 0,5 h. Já o RALF-CH4, com intuito de produzir metano, operou sob TDH fixo de 24 h, submetido à variação da concentração de glicerol de 1, 2, 3, 4, 5 e 7 g.L-1, e portanto, da taxa de carregamento orgânico (TCO) de 1, 2, 3, 4, 5 e 7 kgDQO.m-3.d-1, respectivamente. No RALF-H2, os valores máximos de conteúdo de hidrogênio no biogás (69,2 %), produtividade volumétrica de hidrogênio (1,90 L.h-1.L-1) e rendimento de hidrogênio (0,28 molH2.mol-1glicerolconsumido) foram verificados no TDH de 0,5 h. O 1,3-propanodiol se destacou entre os metabólitos produzidos, com rendimento máximo de 0,57 mol1,3-PDO.mol-1glicerolconsumido, alcançado no TDH de 8 h. No RALF-CH4, o máximo conteúdo de metano no biogás (83,0%) foi verificado na TCO de 1 kgDQO.m-3.d-1, a maior produtividade volumétrica de metano (2,26 L.d-1.L-1) na TCO de 5 kgDQO.m-3.d-1 e o máximo rendimento (0,19 m3H4.kg-1DQOaplicada ou 0,77 molCH4.mol-1glicerolconsumido) na TCO de 4 kgDQO.m-3.d-1. Portanto, os resultados evidenciaram a possibilidade de produção de biocombustíveis e metabólitos de valor agregado utilizando glicerol bruto como substrato no processo da digestão anaeróbia. / The use of renewable fuels has been highlighted in recent years, mainly due to the environmental impacts generated by the use of fossil fuels and their scarcity. Among the renewable fuels highlights the biodiesel, whose production process displays as the main byproduct crude glycerol, which has instigated in recent years, intense debates about a problem of its destination and final disposal. Although it is used in the chemical industry, its impurities content limits its industrial processing. In order to add value to this crude residue and to contribute to the production of bioenergy, this work aimed to evaluate the production of biofuels hydrogen and methane, as well as chemical intermediates from crude glycerol as substrate in anaerobic fluidized bed reactors (AFBR), mesophilic (30°C), using mixed culture inoculum. The AFBR-H2, with the objective of producing hydrogen and soluble metabolites, was submitted to a concentration of 10 g.L-1 of glycerol and a variation of the hydraulic retention time (HRT) of 8, 6, 4, 2, 1 and 0.5 h. The AFBR-CH4, with the intention of producing methane, operated under fixed HRT of 24 h, subjected to the variation of the glycerol concentration of 1, 2, 3, 4, 5 and 7 g.L-1 and, therefore, of the organic loading rate (OLR) of 1, 2, 3, 4, 5 and 7 kgCOD.m-3d-1, respectively. In the AFBR-H2, the maximum values of hydrogen content in the biogas (69.2%), hydrogen volumetric productivity (1.90 L.h-1.L-1) and hydrogen yield (0.28 molH2.mol-1glycerolconsumed) were verified in the HRT of 0.5 h. The 1,3-propanediol was highlighted among the metabolites produced, with a maximum yield of 0.57 mol1,3-PDO.mol-1glycerolconsumed, reached in the TDH of 8 h. In the AFBR-CH4, the higher methane content in the biogas (83.0%) was detected in the OLR of 1 kgCOD.m-3.d-1, a higher volumetric productivity of methane (2.26 L.d-1.L-1) in the OLR of 5 kgCOD.m-3.d-1 and the maximum yield (0.19 m3CH4.kg-1CODapplied or 0.77 molCH4.mol-1glycerolconsumed) in the OLR of 4 kgCOD.m-3d-1. Therefore, the results evidenced the possibility of producing biofuels and value-added metabolites using crude glycerol as a substrate at the anaerobic digestion process.

A utilização de combustíveis renováveis tem se destacado nos últimos anos, principalmente devido aos impactos ambientais gerados pelo uso de combustíveis fósseis e escassez dos mesmos. Dentre os combustíveis renováveis destaca-se o biodiesel, cujo processo produtivo apresenta como principal subproduto o glicerol bruto, que tem instigado nos últimos anos intensas discussões sobre a problemática de sua destinação e disposição final. Apesar de ser utilizado na indústria química, o seu teor de impurezas limita o seu processamento industrial. Buscando agregar valor a esse resíduo bruto e contribuir para a produção de bioenergia, este trabalho teve por intuito avaliar a produção dos biocombustíveis hidrogênio e metano, assim como de intermediários químicos a partir de glicerol bruto como substrato em reatores anaeróbios de leito fluidificado (RALF), mesofílicos (30°C), utilizando inóculo de cultura mista. O RALF-H2, com o objetivo de produzir hidrogênio e metabólitos solúveis, foi submetido à concentração de 10 g.L-1 de glicerol e variação do tempo de detenção hidráulica (TDH) de 8, 6, 4, 2, 1 e 0,5 h. Já o RALF-CH4, com intuito de produzir metano, operou sob TDH fixo de 24 h, submetido à variação da concentração de glicerol de 1, 2, 3, 4, 5 e 7 g.L-1, e portanto, da taxa de carregamento orgânico (TCO) de 1, 2, 3, 4, 5 e 7 kgDQO.m-3.d-1, respectivamente. No RALF-H2, os valores máximos de conteúdo de hidrogênio no biogás (69,2 %), produtividade volumétrica de hidrogênio (1,90 L.h-1.L-1) e rendimento de hidrogênio (0,28 molH2.mol-1glicerolconsumido) foram verificados no TDH de 0,5 h. O 1,3-propanodiol se destacou entre os metabólitos produzidos, com rendimento máximo de 0,57 mol1,3-PDO.mol-1glicerolconsumido, alcançado no TDH de 8 h. No RALF-CH4, o máximo conteúdo de metano no biogás (83,0%) foi verificado na TCO de 1 kgDQO.m-3.d-1, a maior produtividade volumétrica de metano (2,26 L.d-1.L-1) na TCO de 5 kgDQO.m-3.d-1 e o máximo rendimento (0,19 m3H4.kg-1DQOaplicada ou 0,77 molCH4.mol-1glicerolconsumido) na TCO de 4 kgDQO.m-3.d-1. Portanto, os resultados evidenciaram a possibilidade de produção de biocombustíveis e metabólitos de valor agregado utilizando glicerol bruto como substrato no processo da digestão anaeróbia. / The use of renewable fuels has been highlighted in recent years, mainly due to the environmental impacts generated by the use of fossil fuels and their scarcity. Among the renewable fuels highlights the biodiesel, whose production process displays as the main byproduct crude glycerol, which has instigated in recent years, intense debates about a problem of its destination and final disposal. Although it is used in the chemical industry, its impurities content limits its industrial processing. In order to add value to this crude residue and to contribute to the production of bioenergy, this work aimed to evaluate the production of biofuels hydrogen and methane, as well as chemical intermediates from crude glycerol as substrate in anaerobic fluidized bed reactors (AFBR), mesophilic (30°C), using mixed culture inoculum. The AFBR-H2, with the objective of producing hydrogen and soluble metabolites, was submitted to a concentration of 10 g.L-1 of glycerol and a variation of the hydraulic retention time (HRT) of 8, 6, 4, 2, 1 and 0.5 h. The AFBR-CH4, with the intention of producing methane, operated under fixed HRT of 24 h, subjected to the variation of the glycerol concentration of 1, 2, 3, 4, 5 and 7 g.L-1 and, therefore, of the organic loading rate (OLR) of 1, 2, 3, 4, 5 and 7 kgCOD.m-3d-1, respectively. In the AFBR-H2, the maximum values of hydrogen content in the biogas (69.2%), hydrogen volumetric productivity (1.90 L.h-1.L-1) and hydrogen yield (0.28 molH2.mol-1glycerolconsumed) were verified in the HRT of 0.5 h. The 1,3-propanediol was highlighted among the metabolites produced, with a maximum yield of 0.57 mol1,3-PDO.mol-1glycerolconsumed, reached in the TDH of 8 h. In the AFBR-CH4, the higher methane content in the biogas (83.0%) was detected in the OLR of 1 kgCOD.m-3.d-1, a higher volumetric productivity of methane (2.26 L.d-1.L-1) in the OLR of 5 kgCOD.m-3.d-1 and the maximum yield (0.19 m3CH4.kg-1CODapplied or 0.77 molCH4.mol-1glycerolconsumed) in the OLR of 4 kgCOD.m-3d-1. Therefore, the results evidenced the possibility of producing biofuels and value-added metabolites using crude glycerol as a substrate at the anaerobic digestion process.

No description available.

Des nouveaux procédés éco-efficients basés sur une meilleure utilisation des ressources renouvelables sont nécessaires pour assurer la continuité du développement énergétique. La thèse étudie le procédé de gazéification en eau supercritique (T>374°C et P>22,1 MPa) de la biomasse très humide pour l’obtention de l’hydrogène, molécule ayant un potentiel énergétique très intéressant à valoriser avec un impact environnemental très favorable. L’étude porte sur l’application du procédé à la biomasse modèle (solutions de glucose, glycérol et leur mélange) ainsi qu’au bioglycérol, résidu de la fabrication du biodiesel. Les propriétés du solvant et les mécanismes prépondérants développés par l’eau en phase souset supercritique peuvent être contrôlés par les paramètres opératoires imposés au processus : température, pression, concentration en molécules organiques et catalyseur alcalin, temps de réaction... Les études paramétriques des systèmes réactionnels ont été menées dans des réacteurs batch à deux échelles différentes, les phases résultantes étant caractérisées par des protocoles analytiques élaborés et validés dans le cadre de l’étude. Le suivi du milieu réactionnel en batch lors de son déplacement vers l’état supercritique a mis en évidence une conversion avancée des molécules organiques et une identification de certains intermédiaires générés. Parmi les paramètres étudiés, la température et le temps de réaction influent le plus le rendement à l’obtention d’hydrogène en présence de catalyseur (K2CO3) dans les réacteurs batch, rendements de 1,5 et 2 mol d’H2 respectivement par mol de glycérol et de glucose introduites. Les gaz obtenus contiennent des proportions variables d’hydrocarbures légers et du CO2. Environ 75% du carbone est converti en phase gaz et liquide (sous forme de carbone organique et inorganique), le restant étant déposé sous forme solide ou huileuse. L’analyse du solide généré (plus de 90% de C) laisse apparaître différentes phases, y compris la formation de nanoparticules sphériques. Enfin, la gazéification en réacteur continu du glycérol préchauffé a montré de meilleurs rendements en hydrogène que le procédé batch, pendant que celle du bioglycérol demande une évolution du procédé à cause de la précipitation en phase supercritique des sels contenus dans le réactant. En conclusion, la gazéification en eau supercritique de la biomasse peut être considérée comme une alternative intéressante à d’autres procédés physico-chimiques de production de l’hydrogène. L’amélioration du procédé sera possible par son intensification menée en parallèle avec l’utilisation de matériaux plus performants et le contrôle de la salinité de la phase réactante. / Supercritical water (T > 374 ° C and P > 22.1 MPa) gasification of wet biomass for hydrogen production is investigated. This process converts a renewable resource into a gas, which is mainly composed of hydrogen and hydrocarbons with interesting energy potential, and which can be separated at high pressure. In addition, the greenhouse gas effect of the process is zero or negative. Model biomasses (glucose, glycerol and their mixture) and bio-glycerol, residue from bio-diesel production, have been gasified by different processes: two-scale batch reactors (5 mL and 500 mL) and a continuous gasification system. Supercritical water acts as a reactive solvent, its properties can be adjusted by the choice of the experimental (P, T) couple. The operating parameters, e.g. temperature, pressure, concentration of biomass and alkaline catalysts, reaction time… allow favoring certain reaction mechanisms. In order to characterize the processes, specific analytical protocols have been developed and validated. The intermediates, formed during the heating time in the batch reactors, have been identified. Among the investigated operating parameters, temperature and reaction time have the greatest influence on the hydrogen production in batch reactors. In the presence of catalyst (K2CO3), H2 yields of 1.5 mol/mol glucose and 2 mol/mol glycerol have been respectively observed. The obtained gas contains different proportions of light hydrocarbons and CO2. About 75% of the carbon is converted into gas and liquid (in form of organic and inorganic carbon). The conversion leads also to a solid or oily residue. In the generated solid phase (composed over 90% of C), spherical nanoparticles are observed via electronic microscopy. The hydrogen production from glycerol is improved in the continuous process compared to batch reactors, however, bio-glycerol supercritical water gasification requests process improvement due to the precipitation of the salt contained in the reactant. In conclusion, supercritical water gasification of biomass can be considered as an promising alternative process for hydrogen production. The process should be improved by more performing equipments and by the control of the salinity content of the crude biomass.

O presente trabalho de pesquisa teve como objetivo principal avaliar o efeito da carga orgânica volumétrica aplicada (COVA) na produção de hidrogênio usando o reator AnSBBR com recirculação operado em batelada e biomassa imobilizada. Para este efeito, o reator foi operado a 30ºC com dois tempos de ciclo (3 e 4 h), alimentado com três concentrações afluentes (3000, 4000 e 5000 mgDQO.L-1), uma velocidade de recirculação de 30 L.h-1, usando glicerol como única fonte de carbono e a biomassa de uma estação de tratamento de abatedouro de aves. A combinação destes fatores fez com que o reator fosse operado com seis cargas orgânicas volumétricas aplicadas diferentes (7565, 9764, 12911, 10319, 13327 e 16216 mgDQO.L-1.d-1). Os resultados mostraram que não existiu uma tendência clara entre a carga orgânica aplicada e a produção de hidrogênio. Porém, os melhores resultados quanto à produção de hidrogênio foram atingidos quando o reator foi operado com 4 horas de tempo de ciclo e alimentado com uma concentração afluente de 5000 mgDQO.L-1 (COVA de 12911 mgDQO.L-1.d-1), sendo sua produtividade molar média de hidrogênio (PrM) de 67,5 molH2.m-3.d-1. Esta condição também atingiu o melhor rendimento molar de hidrogênio por carga orgânica aplicada RMCAS,m e o melhor rendimento molar de hidrogênio por carga orgânica removida (RMCRS,m), sendo estes de 5,2 e 21,1 molH2.kgDQO-1, respectivamente. Adicionalmente foi estudada a diferença na produção de hidrogênio entre o uso de biomassa pré-tratada e não tratada termicamente, cuja análise de variância (ANOVA) mostrou que a diferença não foi estatisticamente significativa. Finalmente o reator foi operado usando glicerina bruta industrial para comparar os resultados com aqueles obtidos operando com glicerol, observando-se uma clara desvantagem na produção de hidrogênio quando foi usada glicerina bruta. Em geral, o reator AnSBBR operado em batelada sequencial apresentou resultados promissores na produção de hidrogênio usando glicerol como fonte de carbono, porém estudos mais profundos ainda são necessários no intuito de otimizar o sistema para a utilização de glicerina bruta. / This study evaluated the influence of applied volumetric organic load on biohydrogen production in an anaerobic sequencing batch biofilm reactor (AnSBBR) with 3.5 L of liquid medium and treating 1.5 L of glycerin based wastewater per cycle at 30ºC. The reactor was operated with two cycle periods (3 and 4 hours), three influent concentrations (3000, 4000 and 5000 mgCOD.L-1), recirculation rate of 30 L.h-1 and an inoculum from a poultry slaughterhouse. Six applied volumetric organic loads (AVOLCT) were generated from the combination of cycle period and influent concentrations (7565, 9764, 12911, 10319, 13327 e 16216 mgCOD.L-1.d-1). There was not a clear relation between the applied volumetric organic load and hydrogen production. However, the highest hydrogen molar production (MPr: 67.5 molH2.m-3 .d-1) was reached when the reactor was operated with a cycle period of 4 h and an influent concentration of 5000 mgCOD.L-1 (AVOLCT: 12911 mgCOD.L-1. d-1). This condition also reached the highest molar yield per removed load based on organic matter (MYRLC,m: 5.2 molH2.kgCOD-1) and the highest molar yield per applied load based on organic matter (MYALC,m: 21.1 molH2.kgCOD-1). In addition, it was studied whether existed or not a statistical significant difference on molar productivity averages pre-treating and not pre-treating the inoculum. It was observed that this was not statistically significant (p>0.05). Finally, the reactor was operated using crude glycerol as a sole source of carbon to evaluate hydrogen production. The disadvantage on hydrogen production when crude glycerol was used comparing to pure glycerol was clearly observed. The AnSBBR used on hydrogen production experiments operated with pure glycerol as a sole carbon XIV source showed an important potential. Nevertheless, additional studies are required in order to optimize results.

O presente trabalho de pesquisa teve como objetivo principal avaliar o efeito da carga orgânica volumétrica aplicada (COVA) na produção de hidrogênio usando o reator AnSBBR com recirculação operado em batelada e biomassa imobilizada. Para este efeito, o reator foi operado a 30ºC com dois tempos de ciclo (3 e 4 h), alimentado com três concentrações afluentes (3000, 4000 e 5000 mgDQO.L-1), uma velocidade de recirculação de 30 L.h-1, usando glicerol como única fonte de carbono e a biomassa de uma estação de tratamento de abatedouro de aves. A combinação destes fatores fez com que o reator fosse operado com seis cargas orgânicas volumétricas aplicadas diferentes (7565, 9764, 12911, 10319, 13327 e 16216 mgDQO.L-1.d-1). Os resultados mostraram que não existiu uma tendência clara entre a carga orgânica aplicada e a produção de hidrogênio. Porém, os melhores resultados quanto à produção de hidrogênio foram atingidos quando o reator foi operado com 4 horas de tempo de ciclo e alimentado com uma concentração afluente de 5000 mgDQO.L-1 (COVA de 12911 mgDQO.L-1.d-1), sendo sua produtividade molar média de hidrogênio (PrM) de 67,5 molH2.m-3.d-1. Esta condição também atingiu o melhor rendimento molar de hidrogênio por carga orgânica aplicada RMCAS,m e o melhor rendimento molar de hidrogênio por carga orgânica removida (RMCRS,m), sendo estes de 5,2 e 21,1 molH2.kgDQO-1, respectivamente. Adicionalmente foi estudada a diferença na produção de hidrogênio entre o uso de biomassa pré-tratada e não tratada termicamente, cuja análise de variância (ANOVA) mostrou que a diferença não foi estatisticamente significativa. Finalmente o reator foi operado usando glicerina bruta industrial para comparar os resultados com aqueles obtidos operando com glicerol, observando-se uma clara desvantagem na produção de hidrogênio quando foi usada glicerina bruta. Em geral, o reator AnSBBR operado em batelada sequencial apresentou resultados promissores na produção de hidrogênio usando glicerol como fonte de carbono, porém estudos mais profundos ainda são necessários no intuito de otimizar o sistema para a utilização de glicerina bruta. / This study evaluated the influence of applied volumetric organic load on biohydrogen production in an anaerobic sequencing batch biofilm reactor (AnSBBR) with 3.5 L of liquid medium and treating 1.5 L of glycerin based wastewater per cycle at 30ºC. The reactor was operated with two cycle periods (3 and 4 hours), three influent concentrations (3000, 4000 and 5000 mgCOD.L-1), recirculation rate of 30 L.h-1 and an inoculum from a poultry slaughterhouse. Six applied volumetric organic loads (AVOLCT) were generated from the combination of cycle period and influent concentrations (7565, 9764, 12911, 10319, 13327 e 16216 mgCOD.L-1.d-1). There was not a clear relation between the applied volumetric organic load and hydrogen production. However, the highest hydrogen molar production (MPr: 67.5 molH2.m-3 .d-1) was reached when the reactor was operated with a cycle period of 4 h and an influent concentration of 5000 mgCOD.L-1 (AVOLCT: 12911 mgCOD.L-1. d-1). This condition also reached the highest molar yield per removed load based on organic matter (MYRLC,m: 5.2 molH2.kgCOD-1) and the highest molar yield per applied load based on organic matter (MYALC,m: 21.1 molH2.kgCOD-1). In addition, it was studied whether existed or not a statistical significant difference on molar productivity averages pre-treating and not pre-treating the inoculum. It was observed that this was not statistically significant (p>0.05). Finally, the reactor was operated using crude glycerol as a sole source of carbon to evaluate hydrogen production. The disadvantage on hydrogen production when crude glycerol was used comparing to pure glycerol was clearly observed. The AnSBBR used on hydrogen production experiments operated with pure glycerol as a sole carbon XIV source showed an important potential. Nevertheless, additional studies are required in order to optimize results.