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Cellular Basis of Sponge-Sponge AssociationsUnknown Date (has links)
Marine sponges interact and coexist with many different organisms. A two-sponge
association between Amphimedon erina and Geodia gibberosa commonly occurs in the
Florida Keys. Previous studies have only focused on the ecological influence of the
association; they did not examine the cellular basis of the association. This association
between A. erina and G. gibberosa was used in the development of an in vitro model to
further the understanding of the cellular basis of natural sponge-sponge associations. In
this study, sponge cells were cultured individually and in co-cultures and their responses
related to apoptosis, cell death, and proliferation were monitored using high content
imaging. Co-cultured cells of species that form sponge-sponge associations did not have
the same cellular responses compared to co-cultured cells of species that do not form
sponge-sponge associations. Protein expression analyses demonstrated that the model that
was established does not mimic the cellular response of the association in nature, but this
model can be used to test in vitro cellular interactions of sponge species that do not form
associations in nature. In addition, the protein expression data that were obtained revealed that sponges use similar apoptotic pathways as humans and suggest that sponge cells may
shut down cell cycling in order to repair damaged DNA. This research is a small piece to
the puzzle that is sponge cell culture research. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection
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Desenvolvimento de meio quimicamente definido para produção de polissacarídeo capsular em cultivo de Streptococcus pneumoniae sorotipo 14. / Development of a chemically defined medium for capsular polysaccharide production by Streptococcus pneumoniae serotype 14.Ferri, Anne Letícia Silva 14 June 2013 (has links)
Neste trabalho avaliou-se a influência de fontes de carbono (FC) e composições de meio definido no crescimento celular e na produção do polissacarídeo PS14. Em batelada, testou-se como FC glicose, sacarose e frutose em diferentes concentrações. Testou-se também meios com ausência dos aminoácidos asparagina, ácido aspártico, fenilalanina, serina, alanina, treonina, triptofano, lisina e tirosina, das vitaminas/cofatores ácido fólico, piridoxamina, ácido p-aminobenzóico, <font face=\"Symbol\">b-NAD e riboflavina, além bem como da adição de maiores concentrações de aminoácidos identificados como importantes. Em cultivo contínuo foram avaliadas vazões específicas de alimentação (D) de 0,1h-1a 0,5h-1 e a influência das bases nitrogenadas. O meio com sacarose como FC, retirada dos aminoácidos e vitaminas citados e adição do dobro de glicina isoleucina, leucina, valina e o triplo de glutamina levou à maior produção de PS14 (441mg/L). Obteve-se a maior produtividade com D=0,4h-1e a maior quantidade de PS14 com adenina na concentração original no meio de cultura. / In this work we assessed the influence of different carbon sources (CS) and defined medium compositions on cell growth and polysaccharide PS14 production. In bath, glucose, sucrose and fructose were tested at different concentrations. Also, media were tested with absence of the amino acids: asparagine, aspartic acid, phenylalanine, serine, alanine, threonine, tryptophan, lysine, and tyrosine, and vitamins/cofactors: folic acid, pyridoxamine, p-aminobenzoic acid, riboflavin and <font face=\"Symbol\">b-NAD, besides the addition of higher concentration of amino acids identified as important. In continuous cultivation, dilution rates (D) from 0.1 h-1 to 0.5 h-1 were evaluated as well as the influence of nitrogenous bases. The medium containing sucrose as CS, absence of amino acids and vitamins and addition of twice glycine isoleucine, leucine, valine, and triple glutamine led to higher production of PS14 (441mg/L). D of 0.4 h-1 showed higher productivity and adenine in standard concentration produced greater amounts of PS14.
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Purification and identification of a 100 kDa protein, which is tyrosine-phosphorylated by EGF stimulation in SFME cellMurayama, Kaoru 01 May 1997 (has links)
Serum-free mouse embryo (SFME) cells, which were derived
from 16-day-old Balb/c mouse embryo brain, grow in absence of
serum without losing genomic normality or proliferative
potential, and require epidermal growth factor (EGF) for
normal growth. EGF is a well studied mitogen that binds to a
specific receptor on the cell surface membrane to activate
the proliferative signal transduction pathways. The activated
receptor is a tyrosine specific protein kinase, and tyrosine
phosphorylation is one of the important mediators of EGF
receptor (EGFR) signal transduction.
Using anti-phosphotyrosine Western immunoblotting, we
detected a 100 kDa protein which is tyrosine-phosphorylated
in response to EGF in SFME cells. This protein is
constitutively phosphorylated in an SFME cell line which
expresses the neu oncogene. The neu oncogene encodes an
analog protein of EGFR which does not require a ligand for
activation, and neu-transformed SFME cells are tumorgenic in
mice.This protein, p100 was not a fragment of EGFR, and was
not antigenically related to other signal transduction
phosphoproteins of about 100 kDa. We attempted to purify p100
from neu SFME tumor cells for amino acid sequencing. / Graduation date: 1997
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The effect of activated carbon on the organic and elemental composition of plant tissue culture mediumVan Winkle, Stephen C. 05 1900 (has links)
No description available.
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Spirulina production in brine effluent from cooling towersChoonawala, Bilkis Banu January 2007 (has links)
Thesis (M.Tech.:Biotechnology)-Dept. of Biotechnology, Durban University of Technology, 2007
xvi, 185 leaves / Spirulina is a blue-green, multicellular, filamentous cyanobacterium that can grow to sizes of 0.5 millimetres in length. It is an obligate photoautotroph and has a pH growth range from 8.3 to 11.0.The large-scale production of Spirulina biomass depends on many factors, the most important of which are nutrient availability, temperature and light. These factors can influence the growth of Spirulina and the composition of the biomass produced by changes in metabolism. Brine effluent from cooling towers of electricity generating plants may provide an ideal growth medium for Spirulina based on its growth requirements, i.e. high alkalinity and salinity. The aim of this research was to optimise brine effluent from cooling towers by supplementing it with salts, in order to use this optimised effluent in a small open laboratory raceway pond in an attempt to increase the biomass production of Spirulina.
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Detection and enumeration of sublethally-injured Escherichia coli B-41560 using selective agar overlaysSmith, Amanda R. 15 December 2012 (has links)
Quality control procedures during food processing may involve either lengthy enrichment steps, precluding enumeration of bacteria in contaminated food, or direct inoculation of food samples onto appropriate selective media for subsequent enumeration. However, sublethally injured bacteria often fail to grow on selective media, enabling them to evade detection and intervention measures and ultimately threaten the health of consumers. This study compares traditional selective and nonselective agar-based overlays versus two commercial systems (Petrifilm and Easygel) for recovery of injured Escherichia coli B-41560, originally an isolate from ground beef. Bacteria were propagated in tryptic soy broth (TSB), ground beef, or infant milk formula (IMF) to a density of 106-108 CFU/mL, and stressed for six minutes either in lactic acid (pH of 4.5) or heat-shocked for 3 min. at 60°C. Samples were pour- plated in basal layers of either tryptic soy agar (TSA), Sorbitol MacConkey (SMAC), or Violet Red Bile (VRB) agar and resuscitated for 4h prior to addition of agar overlays. Other stressed bacteria were plated directly onto the commercial media Petrifilm and Easygel. Our results indicate that the use of selective and nonselective agar overlays for sensitive recovery and accurate enumeration of E. coli B-41560 is dependent on the stress treatment and food system. These data underscore the need to implement food safety measures that address sublethally- injured bacteria such as E. coli O157:H7, without the use of enrichment steps, in order to avoid underestimation of true densities for target pathogens. / Department of Biology
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The development of a microcomputer controlled variable pathlength turbidimeter /Ortmanis, Andris. January 1986 (has links)
No description available.
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Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cellsVarga Weisz, Patrick D. 05 June 1992 (has links)
Serum-free mouse embryo (SFME) cells are derived
in medium in which serum is replaced with growth
factors and other supplements. They display unusual
properties. They do not lose proliferative potential
or show gross chromosomal aberration upon extended
culture, they depend on epidermal growth factor (EGF)
for survival, and are reversibly growth inhibited by
plasma and serum. In the presence of transforming
growth factor beta (TGF-β) SFME cells express the
astrocyte marker, glial fibrillary acidic protein
(GFAP).
The growth inhibitory activity of human plasma
on serum-free mouse embryo cells was investigated.
Human plasma did not inhibit SFME cells transformed
with the human Ha-ras oncogene. The activity was
present in delipidated plasma and was not dialyzable
against 1 M acetic acid. The activity could be
precipitated by methanol, bound to concanavalin Aagarose
and was retarded by Sephadex G-50 in 200 mM
acetic acid. A fifty to hundred fold purification was
achieved, although the differential inhibition of
untransformed versus transformed cells was lost in the
course of the purification.
Using the technique of differential
screening of a cDNA library a calf serum- and TGF -β-regulated
mRNA species was identified in SFME cells.
This mRNA was approximately 8.5 kilobases in size and
brain-specific. Picomolar quantities of TGF-β caused
an increase of this message in SFME cells within four
hours. This increase was reversed when TGF-β was
removed from the culture medium. / Graduation date: 1993
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Popular culture and literacy learning negotiating meaning with everyday literacies /Jamison, Sally. January 2007 (has links) (PDF)
Thesis (M.I.T.)--The Evergreen State College, 2007. / Title from title screen viewed (6/23/2008). Includes bibliographical references (leaves 80-87).
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Spirulina production in brine effluent from cooling towersChoonawala, Bilkis Banu January 2007 (has links)
Thesis (M.Tech.:Biotechnology)-Dept. of Biotechnology, Durban University of Technology, 2007
xvi, 185 leaves / Spirulina is a blue-green, multicellular, filamentous cyanobacterium that can grow to sizes of 0.5 millimetres in length. It is an obligate photoautotroph and has a pH growth range from 8.3 to 11.0.The large-scale production of Spirulina biomass depends on many factors, the most important of which are nutrient availability, temperature and light. These factors can influence the growth of Spirulina and the composition of the biomass produced by changes in metabolism. Brine effluent from cooling towers of electricity generating plants may provide an ideal growth medium for Spirulina based on its growth requirements, i.e. high alkalinity and salinity. The aim of this research was to optimise brine effluent from cooling towers by supplementing it with salts, in order to use this optimised effluent in a small open laboratory raceway pond in an attempt to increase the biomass production of Spirulina.
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