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Genetics and cytogenetics of Cuphea viscosissima X Cuphea lanceolata hybrid populationsBrandt, Tina L. 15 July 1991 (has links)
Graduation date: 1992
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Simple sequence repeat marker development and fingerprinting in Cuphea lanceolata Ait. and C. viscosissima JacqKarmarkar, Vidyadhar M. 05 August 2003 (has links)
Graduation date: 2004
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GENETIC AND CYTOGENETIC INVESTIGATIONS IN THE GENUS CUPHEAByrd, Abigail Denniston, 1953- January 1987 (has links)
Cuphea (Lythraceae) is a genus of interest as a possible domestic source of medium-chain fatty acids. The genus is in the preliminary phases of domestication. An unsuccessful attempt was made to produce interspecific hybrids between species in several sections of the genus and with varying chromosome numbers. An analysis was made of the time of day and size bud to collect for cytological study of meiotic phases. Chromosome numbers were reported for C. ignea and C. pinetorum. Arm-pairing data were collected for 4 species and one interspecific hybrid. Metaphase I bivalent size was measured for 4 species. A genetic study was done in C. procumbens. Hypotheses regarding the inheritance of flower color, viscid plant hairs, seed coat spots, seed coat hairs and seed dormancy are proposed. A PEG osmoticum pre-germination treatment was found ineffective in breaking dormancy.
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Genetic mapping of Cuphea lanceolata : molecular-marker linkage to quantitative-trait loci affecting seed capric acid, seed oil, and embryo developmentWebb, David M. (David Morton), 1954- 13 November 1990 (has links)
Cuphea is an herbaceous genus having species whose seed
storage lipids are predominantly medium-chain triglycerides (MCTs).
Cuphea lanceolate Ait, and Cuphea viscosissima Jacq. are central to
the breeding of Cuphea as a new commercial source of MCTs. The
objectives of this thesis were to develop a reliable method to
extract DNA from Cuphea, to construct a molecular-marker linkage
map of C. lanceolate, and to evaluate quantitative-trait loci (QTL)
affecting seed capric acid content, seed oil content, and embryo
development in C. lanceolate. We used allozyme and restriction-fragment-
length-polymorphism (RFLP) markers to study Cuphea because
molecular markers are the best method available to investigate the
Mendelian genetics underlying quantitative traits, and are useful
in breeding. Adequate yield of clean DNA is essential for RFLP
mapping. We tried numerous DNA extraction methods that failed to
remove contaminants that interfere with restriction digests of
Cuphea DNA. The method described here was developed to remove
those contaminants and maintain relatively high DNA yields. In
this method, the critical step in purification consists of washing
the DNA with phenol while it is complexed with CTAB and dissolved
in 1 M NaCl. An RFLP and allozyme linkage map of C. lanceolate was
constructed having 37 markers in six linkage groups with a total
distance of 288 cM. Levels of polymorphism were estimated for
three lines of C. lanceolate and one line of C. viscosissima using
84 random genomic clones and two restriction enzymes, EcoRI and
HindIII. Twenty-nine percent of the probes detected RFLPs between
C. lanceolate lines, whereas 63% of the probes detected RFLPs
between C. lanceolate and C. viscosissima lines. Thirty RFLP and
four allozyme markers were used to locate on the C. lanceolata
linkage map QTL affecting seed capric acid content, seed oil
content, and embryo development. Three unlinked QTL explained
19.4% of the phenotypic variation in capric acid content in F₂
seed. Seed oil content and seed weight were measured on seed from
field-grown F₂ plants. Seed weight was indicative of embryo size
(development). Four unlinked QTL explained 33.9% of the phenotypic
variation in embryo size. One of these QTL, which explained 20.3%
of the variation, may have been a chromosomal deletion detected by
a marker having a null allele. Three QTL pleiotropically affected
seed oil by affecting embryo size. The one QTL that only affected
seed oil accounted for 2.8% of the phenotypic variation. / Graduation date: 1991
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Cytology, inbreeding depression, and heterosis of Cuphea Lanceolata AitAli, Md. Shahjahan 02 October 1991 (has links)
Graduation date: 1992
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Acyl-ACP thioesterases and beta-ketoacyl-ACP synthase regulate fatty acid chain length in seed oils of Cuphea wrightiiLeonard, Jeffrey M. 16 December 1996 (has links)
Graduation date: 1997
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The evaluation of cuphea pulchra and cuphea schumannii as potential new ornamental crops for introduction into the Floriculture industryLeopold, Jennifer Hrach 29 September 2004 (has links)
No description available.
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Estudo de Determinação Cromatográfica e Avaliação das Atividades Antifúngica e Anti-hipertensiva de Extratos Obtidos de Cuphea Glutinosa Cham. & Schltdl (lythraceae) / Study of Chromatographic Determination and Evaluation of the Antifungal and Antihypertensive Activities of Extract S Obtained from Cuphea Glutinosa Cham . & Schltdl (lythraceae)Santos, Marí Castro 17 July 2014 (has links)
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Previous issue date: 2014-07-17 / O gênero Cuphea, popularmente conhecido no Brasil por “sete-sangrias”, tem seu uso
medicinal reconhecido devido aos efeitos diurético, hipotensor e cardioprotetor. No sul do
Brasil, em região característica do bioma Pampa, foi encontrada a espécie Cuphea glutinosa
Cham. & Schltdl. Embora o uso popular, esta espécie é pouco descrita na literatura. O
presente trabalho tem como objetivos o estudo da composição química dos extratos de C.
glutinosa e a avaliação das atividades antifúngica e anti-hipertensiva. O material vegetal foi
coletado na cidade de Uruguaiana (RS, Brasil), identificado e depositado em herbário. Após
secagem e trituração do material vegetal, foram obtidos os extratos hidroetanólicos através de
maceração exaustiva com etanol 40% (v/v) para folhas e etanol 70% (v/v) para raízes. Para a
infusão, utilizou-se água a 80oC. As análises cromatográficas foram realizadas em
equipamento cromatógrafo a líquido Prominence Shimadzu, em técnica por CLAE e CLUE.
Utilizou-se sistema de fase reversa, eluição por gradiente com fase móvel composta por
acetonitrila:metanol (4:1) e ácido fórmico 0,1% pH 3,0, coluna C18 analítica e fast, e
detecção por UV-DAD e ESI-MS. Os teores de polifenóis totais e de flavonóides foram
determinados por método colorimétrico, seguindo metodologia padronizada. A atividade
antifúngica in vitro foi realizada utilizando o método de microdiluição em caldo,
determinando-se a CIM, in-vitro, contra diferentes isolados clínicos. Para avaliação do
potencial anti-hipertensivo in vivo, foram realizadas medições da pressão sanguínea pelo
método de monitoramento hemodinâmico invasivo, através da inserção de cateter na artéria
carótida. Os resultados de teor de fenólicos totais indicaram predominância destes
componentes em extratos obtidos de folhas e por maceração, conforme os valores obtidos:
1,8501 mg EAG/mL (folhas) e 0,8467 mg EAG/mL (raízes) para infusão, e 3,7284
mgEAG/mL (folhas) e 2,6266 mg EAG/mL (raízes) para maceração. Quanto ao teor de
flavonóides, os resultados quantitativos foram: 7,0959 mg/g (folhas) e 0,5664 mg/g (raízes)
para a infusão, e 7,9511 mg/g (folhas) e 0,5994 mg/g (raízes) para maceração. Na análise
cromatográfica, os extratos obtidos das folhas de C. glutinosa apresentaram picos
cromatográficos bem separados, em perfil reprodutível. Na determinação por CLUE-MS, os
dados de íon molecular e fragmentos de massa indicaram a composição predominante em
flavonóides, sugerindo-se os componentes quercetina-3-O-glicosídeo, quercetina-3-
arabinosídeo, quercetina-3-glicuronídeo, isoramnetina e quercetina-5-O-β-glicopiranosídeo.
Para o potencial antifúngico, os extratos das folhas e raízes apresentaram atividade in vitro
contra Candida parapsilosis, Candida tropicalis e Trichosporon asahii, com CIM variando na
faixa de 1,9-62,5 μg/mL. Nos testes hemodinâmicos realizados, os extratos das folhas não
apresentaram efeito significativo sobre a pressão arterial. A identificação dos componentes
em C. glutinosa, derivados de quercetina, torna promissora novas investigações a fim de
aprofundar o conhecimento a respeito desta espécie, em especial na busca de respostas para a
relatada ação anti-hipertensiva. / The Cuphea genus, popularly known in Brazil as "sete-sangrias", is used traditionally
due the diuretic, hypotensive and cardioprotective effects. In southern Brazil, in characteristic
region of Pampa biome, it was found the species Cuphea glutinosa Cham. & Schltdl.
Although used popularly, this species is few reported in the literature. The present work
aimed to study the chemical composition of extracts from C. glutinosa and to evaluate the
antifungal and anti -hypertensive activities. The plant material was collected in the city of
Uruguaiana (RS, Brazil), identified and deposited in a herbarium. After dryness and milling,
the hydroethanolic extracts were obtained through exhaustive maceration using ethanol 40%
(v/v) for leaves and ethanol 70% (v/v) for roots. The infusions were prepared using water at
80 °C. The chromatographic analyses were performed in liquid chromatography Prominence
Shimadzu, for HPLC and UPLC assays. The method was conducted using reverse phase
system, gradient elution with mobile phase composed by acetonitrile:methanol (4:1) and
formic acid 0.1% pH 3.0, C18 analytical and fast column, and detection by UV-DAD and MS.
The polyphenols and flavonoids contents were determined by colorimetric method. The in
vitro antifungal activity was conducted by using the broth microdilution method, determining
the MIC against different clinical isolates. For evaluation of in vivo anti-hypertensive
potential, the blood pressure was measured by the method of invasive hemodynamic
monitoring, through of insertion the catheter into the carotid artery. The results of phenolic
content indicated the high concentration of these compounds in leaves extracts obtained by
maceration: 1.8501 mgEAG/mL (leaves) and 0.8467 mgEAG/mL (roots) for infusion, and
3.7284 mgEAG/mL (leaves) and 2.6266 mgEAG/mL (roots) for maceration. For flavonoids,
the contents were: 7.0959 mg/g (leaves) and 0.5664 mg/g (roots) for infusion, and 7.9511
mg/g (leaves) and 0.5994mg/g (roots) for maceration. In the chromatographic analyses, the
leaf extracts from C. glutinosa presented chromatographic peaks well separated and
reproducible. In the determination by UPLC-MS, the molecular ion and mass fragments
indicated the predominant composition in flavonoids, suggesting the compounds quercetin-3-
O-glucoside, quercetin-3-arabinoside, quercetin-3-glucuronide, isorhamnetin and quercetin-5-
O-β-glucopiranoside. For the antifungal potential, the leaf and roots extracts presented
activity against Candida parapsilosis, Candida tropicalis e Trichosporon asahii, with MIC
values ranging 1,9-62,5 μg/mL. In the hemodynamic tests performed, the leaves extracts did
not present significant effect in the arterial pressure, although a tendency in pressure reduction
could be observed. The identification of quercetin derivatives in C. glutinosa becomes
promisor further investigations about this species, mainly in respect to the anti-hypertensive
action.
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