Thesis (doctoral)--Eberhard-Karls-Universität, Tübingen, 1935.

No description available.

No description available.

No description available.

Thesis (doctoral)--University of Copenhagen.

No description available.

Introduction Previous studies on the glandular odontogenic cyst (GOC) have largely focused on the application of immunohistochemistry for determining how the GOC lining epithelium compares with that of other odontogenic cysts. Studies on the histochemical composition of the mucous cells in the GOC are, however, lacking. This study therefore aimed to determine the mucin phenotype of the mucous cells in the GOC and compared these findings with the mucous cells in the epithelial linings of other odontogenic cysts and with normal salivary gland mucous acinar cells. Materials and Methods Twenty-seven cases made up of 10 GOCs, 9 dentigerous cysts (DC) with mucous cells and 8 radicular/residual radicular cysts (RC) with mucous cells were stained using the combined alcian blue pH 2.5-PAS (AB-PAS) histochemical technique. AB-PAS allows for differentiation between acidic- (type I mucous cells), neutral- (type II mucous cells) and mixed mucin-containing cells (type III mucous cells). Submandibular, sublingual and palatal salivary gland tissue was also subjected to AB-PAS staining. The odontogenic cysts and salivary glands were evaluated for the frequency of type I, II and III mucous cells in these tissues. Results There were significant differences between the level of type I, type II and type III mucous cells within each of the three cyst types; GOC (p=0.006), DC (p=0.0004), RC (p=0.0017). There were no significant differences in the cell counts for each mucous cell type between the 3 cyst types;type I mucous cells (p=0.54); type II mucous cells (p=0.73) and type III mucous cells (p=0.97).All 3 odontogenic cysts showed a predominance of type III mucous cells and this mirrored the mucin phenotype of the submandibular and sublingual salivary glands. Conclusion The mucin phenotype of the GOC is shared by DC and RC with mucous metaplasia. The overlapping mucin phenotypes of the different odontogenic cysts unfortunately does not support the use of the AB-PAS stain as a potential histochemical marker to distinguish between the GOC and other odontogenic cysts with mucous metaplasia. Similarities in the mucin phenotype between odontogenic cysts, submandibular and sublingual salivary glands may suggest a common ectodermal histogenetic origin for the mucous cells in odontogenic cysts and major salivary glands.

Harmful algal blooms (HABs) along the Florida coast have been reported for many decades. Karenia brevis is a red tide species on the West Florida Shelf, producing a suite of toxins called brevetoxins that adversely affect marine organisms and humans. Dinoflagellate cysts have been studied as a tool for red tide studies since the location where cysts accumulate in the sediments and the size of the seed beds can be important for potential blooms. However, little attention has been paid on the dinoflagellate cysts on the West Florida Coast. This study describes the distribution of dinoflagellate cysts along the West Florida Coast and proposes the possibility of Karenia brevis cysts. Samples were collected with a box corer July 17-29 and October 5-17, 2009. Overall cyst concentrations are low. The sediments along the West Florida Coast compose of coarse-sized grains that generate large-sized pores. Thus, cysts in the coarse-grained sediments might easily move along with turbulence or water movements flowing above the sediments. Cyst concentrations gradually increased shoreward. The hydrographic features along the West Florida Coast are influenced by the coastal current. Southward coastal current flowed during two cruises might drive a subsequent offshore Ekman transport which might lead to a coastal upwelling, thereby transporting bottom sediments closer to the coast. Thus, cyst concentration was higher inshore compared to that offshore. The average concentration of heterotrophic dinoflagellate cysts was higher in July than that in October, whereas autotrophic dinoflagellate cysts did not have a noticeable difference between July and October. The heterotrophic group is dominated by a protoperidinioid group that mainly feeds on diatoms. The abundance of protoperidinioid was higher in July than in October and other groups have similar abundance between two periods. The highest abundance of diatoms is in June, July and August, whereas the lowest was in October, November and May. Therefore, the decrease in the abundance of protoperidinioid cysts correlates with the lower food supply for their motile cells. Based on a morphological similarity to a Karenia brevis cyst detected in culture by Walker (1982) and a morphological difference from other species belonging to the same genus, cysts that are probably Karenia brevis have been identified.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1967. / Typescript. Vita. Description based on print version record. Includes bibliographical references.

Osteoarthritis is a chronic degenerative joint disease that affects the whole joint, including the articulating surfaces and capsular tissues in and around the joint (Hügle and Geurts 2016). Osteoarthritis currently impacts 27 million Americans (Racine 2015). Two prominent features in osteoarthritis that are correlated with greater pain and reduced function are MRI-identified bone marrow lesions and CT-identified subchondral bone cysts (Kumar et al. 2013). At this time, the relationship between bone marrow lesions and subchondral bone cysts has not been confirmed. Nor has the nature of the tissues associated with these clinical signs been fully characterized, pointing to the need for further research to identify the composition of subchondral bone cysts as these cysts could be a potential target for therapeutic intervention. This study focused on characterizing the tissue content of subchondral bone cysts in osteoarthritic femoral heads. Osteoarthritic femoral heads were collected from male and female patients, ranging in age from 43-72 years old, who underwent total hip replacement. After surgery, the femoral heads were fixed in 4% paraformaldehyde and scanned via micro-computed tomography (MicroCT). The MicroCT images were used to identify regions in each head containing large subchondral bone cysts, the primary compressive bone, and anterior cartilage. These regions were mapped to the actual femoral head and guided the dissection of the femoral head. Samples from each of the regions were decalcified, embedded in paraffin, and cut into 5-micron-thick histological sections which were then mounted onto slides. Each section was stained in Safranin-O/Fast green and hematoxylin and eosin to visualize the tissues present. Immunohistochemistry with anti-CD31 was carried out on selected slides to identify blood vessels. The samples within all three regions of the femoral head were graded histologically for the presence of subchondral bone cysts, whether cartilage was within the cyst region, if the histological section matched its corresponding MicroCT image, if the articular cartilage was fibrillated, the presence of sclerotic bone, the presence of osteophytes, and the presence of blood vessels. It was clear that many of these samples were in later stages of osteoarthritis considering most samples exhibited all of the above characteristics, contained fibrous tissue, and had little normal fatty marrow. Typically, subchondral bone cysts presented beneath fibrillated and degenerated articular cartilage, contained fibrous tissue that was much more intensely vascularized and innervated as compared to normal fatty marrow, and was surrounded by sclerotic trabecular bone. In some cases, osteophytes also formed at the articular surface beneath areas of degenerated cartilage. Some subchondral bone cysts contained cartilage and even smooth muscle cells in addition to fibrous connective tissue. The varied location of the subchondral bone cysts shows the need for further research as to how their etiologies develop. The content of the subchondral bone cysts suggests that areas with disrupted trabeculae become intensely vascularized to allow the influx of inflammatory materials and mesenchymal stem cells to lay down fibrous tissue and thicken the surrounding trabeculae to stabilize the weakened microstructure of the femoral head. By gaining a comprehensive understanding of the pathology of osteoarthritis and of subchondral bone cysts in particular, the progression of the disease can be more firmly established and potential new treatments can be developed.