Apoptosis represents one of the cell death mechanisms which is realized after the application of taxanes in breast cancer cell lines. Apoptosis induction can be principally triggered either by outer or inner pathway. The aim of the diploma thesis is to contribute to the elucidation of role and mechanisms of the inner mitochondrial pathway of apoptosis induction after taxane application (paclitaxel and SB-T-1216) employing a model of breast carcinoma cell lines SK- BR-3 (nonfunctional p53, functional capase-3) and MCF-7 (functional p53, nonfunctional caspase-3). Specifically, we tested the effect of both employed taxanes on mitochondrial membrane potential, ROS level and the expression and localization of proteins regulating inner mitochondrial pathway. Taxane application resulted in mitochondrial membrane dissipation in SK-BR-3 cell line. However, this was not shown in MCF-7 cell line. We found no changes in Bax and Smac/DIABLO expression after taxane application in both tested cell lines. There was a decrease of Bid expression after taxane application in SK-BR-3 line, but not in MCF-7 line. Taxane application did not lead to the translocation of Bax and Bid (tBid) proteins from cytosol to mitochondria in both tested cell lines. Similarly, there was no Smac/DIABLO release from mitochondria to...

The involvement of mitochondrial signaling in mammalian PCD regulation has been examined extensively via biochemical analyses and cellular studies in vitro. However there still exist considerable gaps in our knowledge regarding its contribution in specific tissues and cell types during mammalian development in vivo. In addition, given the numerous pathologic conditions associated with aberrant PCD, modulation of this signaling process represents an attractive target for therapeutic intervention. In this thesis I have therefore examined the regulation of mitochondrion-mediated PCD signaling as it pertains to several forms of developmental and injury-induced cell death. In the first component of the thesis I have examined the differential sensitivity of Bcl2 on the survival of motor neuron populations from two distinct developmental origins (alpha and gamma motor neurons), demonstrating that gamma motor neurons are preferentially affected in Bcl2 null mice. Thus, Bcl-2 plays a critical in vivo in regulating subtype-specific motor neuron survival during development. In the second study I have demonstrated that a major portion of the neuroprotective effect exerted by the immunophilins cyclosporin A and FK-506 are mediated through calcineurin signaling; rather than MOMP-mediated events as previously held. Additional findings of this study demonstrated the first neuroprotective effects of the pyrethroid insecticide cypermethrin and calcineurin-mediated control of Bad phosphorylation. Such findings establish a link between calcineurin signaling and mitochondrion-mediated cell survival. The above studies established critical features of mitochondrion-mediated PCD in regulating survival of several neuronal subpopulations. I therefore followed these studies with an examination of how post-mitochondrial PCD signaling is regulated following MOMP permeabilization. Specifically I examined regulation of the Smac-IAP-caspase axis, investigating how combinatorial deletion of Casp3 and Diablo alter PCD progression in mouse embryonic fibroblasts. Using a series of injury stimuli in the context of biochemical and cellular analyses I have developed a model of how endogenous Smac/DIABLO regulates executioner caspase activity. Collectively these studies elucidate key aspects of mitochondrial signaling during both developmental and injury-induced PCD in vivo.

The involvement of mitochondrial signaling in mammalian PCD regulation has been examined extensively via biochemical analyses and cellular studies in vitro. However there still exist considerable gaps in our knowledge regarding its contribution in specific tissues and cell types during mammalian development in vivo. In addition, given the numerous pathologic conditions associated with aberrant PCD, modulation of this signaling process represents an attractive target for therapeutic intervention. In this thesis I have therefore examined the regulation of mitochondrion-mediated PCD signaling as it pertains to several forms of developmental and injury-induced cell death. In the first component of the thesis I have examined the differential sensitivity of Bcl2 on the survival of motor neuron populations from two distinct developmental origins (alpha and gamma motor neurons), demonstrating that gamma motor neurons are preferentially affected in Bcl2 null mice. Thus, Bcl-2 plays a critical in vivo in regulating subtype-specific motor neuron survival during development. In the second study I have demonstrated that a major portion of the neuroprotective effect exerted by the immunophilins cyclosporin A and FK-506 are mediated through calcineurin signaling; rather than MOMP-mediated events as previously held. Additional findings of this study demonstrated the first neuroprotective effects of the pyrethroid insecticide cypermethrin and calcineurin-mediated control of Bad phosphorylation. Such findings establish a link between calcineurin signaling and mitochondrion-mediated cell survival. The above studies established critical features of mitochondrion-mediated PCD in regulating survival of several neuronal subpopulations. I therefore followed these studies with an examination of how post-mitochondrial PCD signaling is regulated following MOMP permeabilization. Specifically I examined regulation of the Smac-IAP-caspase axis, investigating how combinatorial deletion of Casp3 and Diablo alter PCD progression in mouse embryonic fibroblasts. Using a series of injury stimuli in the context of biochemical and cellular analyses I have developed a model of how endogenous Smac/DIABLO regulates executioner caspase activity. Collectively these studies elucidate key aspects of mitochondrial signaling during both developmental and injury-induced PCD in vivo.

Made available in DSpace on 2016-05-17T16:51:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-11. Added 1 bitstream(s) on 2016-05-17T16:54:56Z : No. of bitstreams: 1 000864019.pdf: 1052012 bytes, checksum: 30868035736293f5c3dc2f8b6388e85b (MD5) / A presente dissertação de Mestrado tem como título Macário e La cruz del diablo: a caracterização da figura do diabo na literatura fantástica e discute a forma como a figura do diabo se estabelece nas duas obras fantásticas, de autoria de Álvares de Azevedo e de Gustavo Adolfo Bécquer, respectivamente. Para estudar estas duas obras e estabelecer semelhanças e diferenças entre elas, será estudada a figura do diabo, criatura bastante utilizada em obras literárias, especialmente fantásticas; o gênero fantástico, pois ambas as obras possuem características que permitem inseri-las neste. Serão estudadas também algumas características do Romantismo, pois ambas as obras foram escritas durante este período. Outras teorias como as do conto, do teatro, da lenda e da literatura comparada serão brevemente incluídas quando necessárias para a análise das obras / This Masters dissertation called Macário e La cruz del diablo: the figure of the devil's characterization in fantastic literature, discourses about the way as the figure of the devil sets in two Álvares de Azevedo and Gustavo Adolfo Becker 's literary works, respectively. To study these two literay works and find similarities and differences between them, it will be study the figure of the devil, a creature widely used in literary works, especially in fantastic literay works; fantastic literary genre, because both literay works have features which allow insert them in fantastic literay genre. It will be also studied some features of Romanticism, whereas the two literary works studied here, were written during the romantic period. Other theories such as the tale, the theater, the legend and the comparative literature, will be briefly included when necessary, for the analysis of the literay works

No description available.

No description available.

Submitted by Maria Aparecida (cidazen@gmail.com) on 2017-11-27T20:18:19Z No. of bitstreams: 1 Isa Prazeres Pestana.pdf: 1967644 bytes, checksum: 126e377e02ce70433d5512a91248e655 (MD5) / Made available in DSpace on 2017-11-27T20:18:19Z (GMT). No. of bitstreams: 1 Isa Prazeres Pestana.pdf: 1967644 bytes, checksum: 126e377e02ce70433d5512a91248e655 (MD5) Previous issue date: 2017-07-10 / Este estudio tiene como objetivo analizar el discurso y las prácticas religiosas de la Igreja Universal do Reino de Deus, destacando la guerra contra el diablo y el proceso de demonización de los cultos afro-brasileños, en el período entre 1992 - en la primera edición del diario Folha Universal y 2017 - año de la realización de las entrevistas. Además, se busca comprender la figura del diablo en el imaginario cristiano, que tuvo presencia marcada desde el período medieval y resurge como elemento central en el discurso de la Igreja Universal en el siglo XX, período analizado por algunos investigadores como: de la secularización y del desencanto. Las fuentes utilizadas durante la investigación fueron orales (entrevistas), documentación escrita (periódicos, procesos, publicaciones), bibliografía especializada sobre la temática. / This study aims to analyze the discourse and religious practices of the Church Universal of the Kingdom of God, highlighting the war against the devil and the demonization of Afro-Brazilian cults, in the period between 1992 - in the first edition of the newspaper Folha Universal and 2017 - year of the interviews. In addition, we are looking for understand the figure of the devil in the Christian imaginary, which had a marked presence since the medieval period and resurges as a central element in the discourse of the Universal Church in the twentieth century, a period analyzed by some researchers such as: secularization and disenchantment The sources used during the research were oral (interviews), written documentation (newspapers, processes, publications), specialized bibliography on thematic. / Este estudo tem como objetivo analisar o discurso e as práticas religiosas da Igreja Universal do Reino de Deus, destacando a guerra contra o diabo e o processo de demonização dos cultos afro-brasileiros, no período compreendido entre 1992 – ano da primeira edição do jornal Folha Universal e 2017 – ano da realização das entrevistas. Além disso, busca-se compreender a figura do diabo no imaginário cristão, que teve presença marcada desde o período medieval e ressurge como elemento central no discurso da Igreja Universal no século XX, período analisado por alguns pesquisadores como: da secularização e do desencantamento. As fontes utilizadas durante a pesquisa foram orais (entrevistas), documentação escrita (jornais, processos, publicações), bibliografia especializada sobre a temática.

This dissertation investigates the literary and cinematic use of the child’s perspective to present the Mexican Revolution and the Spanish Civil War and their aftermath in several Mexican, Spanish, and international (Mexican-Spanish collaborative) narratives of the 20th and early 21st Centuries written by adult authors and filmmakers, and targeted for adult audiences. The Mexican narratives are Cartucho and Las manos de mamá by Nellie Campobello, Balún Canán by Rosario Castellanos, and Bandidos, a film by Luis Estrada; selected Spanish works are El espíritu de la colmena by Víctor Erice, Cría cuervos by Carlos Saura, and El sur by Adelaida García Morales; and both international works are films by Guillermo del Toro, El espinazo del diablo and El laberinto del fauno. I attempt to determine the textual or cinematic function of the child as first person (homodiegetic) narrative viewer in these works, and I study the different ways in which this child’s point of view is constructed in order to depict the overwhelming tragedy of war. I note patterns and diversities in subject matter presented by the narrative voice, and observe the characteristics of the child narrative viewer’s world and priorities (as presented by the authors and filmmakers), paying careful attention to how each perceives and understands his or her country’s violent upheaval and its aftermath. The theoretical framework of this investigation draws mainly from trauma theory, Gothic studies, and the tradition of the fairy tale. I illustrate how within the war narrative in addition to the author’s/filmmaker’s desire to recreate the sentiment that a child would evoke in adult readers and viewers, the child narrative viewer is employed for three main reasons: to play upon or against preexisting notions of the child’s innocence; to represent (possibly subversively) the nation; and as therapeutic means of returning to a paradise lost or creating a paradise never experienced. / text

The aim of this project has been to investigate whether there are correlations to be found between gut microbes and serum metabolites, which could be involved in the development of epilepsy. To do so, metabolomics data containing metabolites and metagenomics data containing bacteria have been integrated and used in a pipeline utilizing the software package DIABLO in R Studio. DIABLO stands for Data Integration Analysis for Biomarker discovery using Latent cOmponents and utilizes multi-block pls-da to integrate multiple omics data sets to find potential biomarkers. The results in this project are mainly divided into two groups, the first group being from taking samples at an early time point, where subjects have not yet developed symptoms of epilepsy and the second group being from taking samples at a late time point, where the subjects have developed epilepsy. To find biomarkers in the data used for the integration, two subgroups are of highest interest, namely subgroup PTE, which is the group that develops epilepsy symptoms after an induced trauma to the brain, as well as subgroup TBI which do not develop epilepsy symptoms after an induced trauma to the brain. Results from the early time point suggests that bacteria such as those from Phelethenecus, Christenselellales, Ventrimonas, Ruminococcaceae and Acetatifactor, as well as metabolites such as LPC 17:0, Indole and Indole-3-carboxyaldehyde might be of interest in finding biomarkers previous to the development of epilepsy after induced brain trauma.  Results from the late time point suggests that bacteria such as those from Muribaculaceae and Avidehalobacter, as well as metabolites such as Dioctyl sulfosuccinate, Canrenone, LPC 18:0, Uric acid, Arjunolic acid and Pseudouridine might be of interest in finding underlying mechanisms behind the existing condition of epilepsy. The hope is that findings in this paper might aid in future development of knowledge behind this disease as well as its underlying mechanisms.