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The White-bellied Sea-Eagle Haliaeetus leucogaster is widespread in Australia, but has been the subject of conservation concern due to suggested localised declines and extinctions. Regionalised monitoring programmes have addressed some aspects of local concern, however a broader approach is needed to gain an understanding of large-scale processes affecting long-term persistence at scales equivalent to the species Australian range. Ultimately, the ability to predict change in population size over time accurately depends on the scale of analysis. By necessity, ecological studies using direct sampling techniques are often made across spatial scales smaller than a species geographic range and across relatively short time frames. This seems counter-intuitive considering that long-term species persistence is often dependent on large-scale processes. The principal aim of this thesis was to identify historical and contemporary forces responsible for the current pattern of population structure in H. leucogaster. This required a multi-scale approach, and the resulting research uses genetic, distributional and morphometric data. Haliaeetus leucogaster is a large territorial raptor that historically has been associated with coastal regions, lakes and perennial river systems. It has an extensive worldwide distribution from the western coast of India throughout the Indomalaysian region, Papua New Guinea and Australia. By virtue of the species' large-scale distribution, in Australia it is fairly cosmopolitan in its use of habitat and prey types. Haliaeetus leucogaster is monomorphic for adult plumage colouration, but in body size displays reversed sexual dimorphism with female birds significantly larger. A discriminant function based on 10 morphometric characters was 100% effective in discriminating between 19 males and 18 females that had been sexed using molecular genetic methods. Re-classification using a jackknife procedure correctly identified 92% of individuals. The discriminant function should be a viable alternative to genetic sexing or laparoscopy for a large proportion of individuals within the Australo-Papuan range of this species; and can also be used to identify a small proportion of "ambiguous" individuals for which reliable sexing will require those other techniques. I used mitochondrial (mtDNA) control region sequence data to investigate the current distribution of genetic variation in this species at the continental level and within and between specified regional units. I was specifically interested in identifying breaks in genetic connectivity between the west and east of the continent and between Tasmania and the Australian mainland. Overall, genetic diversity was low and there was no significant level of genetic subdivision between regions. The observed genetic distribution suggests that the population expanded from a bottleneck approximately 160 000 years ago during the late Pleistocene, and spread throughout the continent through a contiguous range expansion. There is insufficient evidence to suggest division of the population into different units for conservation management purposes based on the theoretical definition of the 'evolutionary significant unit'. It is clear from the analysis that there are signatures of both historical and contemporary processes affecting the current distribution. Given the suggestion that population expansion has been relatively recent, additional sampling and confirmation of the perceived pattern of population structure using a nuclear marker is recommended to validate conservation monitoring and management at a continental scale. To determine the existence of perceived population declines across ecological time scales, I analysed the Australian Bird Atlas Data to identify the extent and pattern of change in range and density of the species between three Atlas Periods (1901-1976, 1977-1981 and 1998-2001) using a new standardised frequency measure, the Occupancy Index (OI) for 1° blocks (approx. 100km2) across the continent. At the continental scale, there was no significant difference in the spatial extent of occupancy between Atlas Periods. However, there were considerable changes in frequency and range extent between defined regions, and there were distinct differences in the pattern of change in OI between coastal and inland blocks over time. Coastal blocks showed much more change than inland blocks, with a clear increase in the use of coastal blocks, accompanied by a decrease in inland blocks, during the 1977-1981 Atlas Period, relative to both other Atlas Periods. The over-riding factor associated with distributional shifts and frequency changes was apparently climatic fluctuation (the 1977-1981 period showing the influence of El Nino associated drought). The impression of abundance was strongly dependent on both the temporal and spatial scale of analysis. To test for correspondence between geographic variation in morphology and geographic variation in mtDNA I analysed morphometric data from 95 individuals from Australia and Papua New Guinea. First, the degree of morphometric variation between specified regions was determined. This was then compared with the pattern of genetic differentiation. There was a strong latitudinal cline in body dimensions. However, there was no relationship between morphometric variation and patterns of genetic variation at least for mtDNA. Females showed a pattern of isolation by distance based on morphometric characters whereas males did not. Three hypotheses to explain the pattern of morphometric variation were considered: phenotypic plasticity, natural selection and secondary contact between previously isolated populations. I conclude that the pattern of morphometric variation is best explained by the suggestion that there is sufficient local recruitment for natural selection to maintain the observed pattern of morphometric variation. This implies that gene flow may not be as widespread as the mtDNA analysis suggested. In this instance either the relatively recent colonisation history of the species or the inability of the mtDNA marker to detect high mutation rates among traits responsible for maintaining morphometric variation may be overestimating the levels of mixing among regions. As might be expected given the physical scale over which this study was conducted, the pattern of genetic, morphometric and physical distribution varied dependent on the scale of analysis. Regional patterns of genetic variation, trends in occupancy and density and morphometric variation did not reflect continental patterns, reinforcing the contention that extrapolation of data from local or regional levels is often inappropriate. The combined indirect methodologies applied in this study circumvent the restrictions imposed by direct ecological sampling, because they allow survey across large geographic and temporal scales effectively covering the entire Australian range of H. leucogaster. They also allow exploration of the evolutionary factors underpinning the species' current distribution.

Thesis (M.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 68-82). Also available on the World Wide Web.

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Global amphibian declines have been attributed to numerous and often synergistic causes, such as invasive species, pathogens, and ultraviolet-B (UV-B) radiation. The effects of these stressors are context dependent and can vary with location, species, and populations. As sensitivity to UV-B has shown inconsistencies across amphibian taxa, it can be expected that variation also occurs between populations of a single species. High elevation populations of the long-toed salamander (Ambystoma macrodactylum) face exponentially higher UV-B radiation levels relative to low elevation populations and these levels are predicted to increase in conjunction with continued ozone depletion. We hypothesized that breeding long-toed salamander females at high elevations have modified oviposition behavior to better protect embryos from UV-B induced damage. In addition, we hypothesized that long-toed salamander embryos at high elevation would exhibit elevated photolyase activity, a photo-reactivating enzyme that repairs UV-B radiation-induced damage to DNA. We predicted that this behavioral defense strategy would be employed together with an elevated physiological response as a correlated defense response to increased levels of UV-B radiation in high elevation populations. We surveyed high and low elevation long-toed salamander breeding sites throughout Oregon to quantify oviposition site characteristics and associated UV-B profiles. We simultaneously collected embryos for quantification of photolyase activity in a bacterial transformation assay. We found significant differences in oviposition behavior across elevations, with high elevation breeding females ovipositing in deeper water and using UV-B protective refugia. Oviposition sites at low elevations, however, were most often found in UV-B exposed microhabitats located at the surface of the water. This population difference in oviposition behavior resulted in a standardization of UV-B and temperature conditions for long-toed salamander embryos across elevation. In contrast, we found no population differentiation in photolyase activity between high and low elevation breeding sites. This indicates that behavioral selection for UV-B protected oviposition substrates may either be negating the need for increased photolyase activity in long-toed salamander embryos, or that populations lack the capacity to adapt a heightened physiological response to UV-B at high elevations. Together, these results show how trade-offs in physiology and behavior are a unique adaptation to a significant environmental stressor. Further research into the susceptibility of amphibian species to changing environmental conditions may help to demonstrate the effectiveness of correlated trait responses and plasticity in behavior, and species persistence under changing climate regimes. / Graduation date: 2012

Alien fish have been implicated in the decline of Rana draytonii (California red-legged frog) and Pseudacris regilla (Pacific treefrog) populations. Micropterus salmoides (largemouth bass) is a common sport fish that has been introduced into the sympatric range of these two anuran species; however, no studies have investigated the individual influence that this alien fish has on these two species. I conducted M. salmoides control or eradication experiments during a four year study in two ponds within the Transverse Mountain Range of Santa Barbara County, California. Changes in the densities of all life stages of R. draytonii and P. regilla were assessed through repeated visual encounter surveys. In response to the manipulations of the M. salmoides populations there was an increase in the density of both species. For R. draytonii, this salient increase in density was recorded in the larval and metamorph life stages. For P. regilla, a slight, yet steady increase in the density of adult, metamorph, and larval life stages was recorded. My results suggest that M. salmoides can be controlled or eradicated from lentic waterbodies and provides reasonable evidence that the negative effects of this species on R. draytonii and P. regilla populations can be minimized or reversed. The reversal of these effects may translate into increases in the densities and potential recruitment of these two native anurans. Therefore, my results provide tentative support that the control or eradication of M. salmoides where they cohabitate with these two species may be a viable conservation measure for R. draytonii and P. regilla populations. Since my study was performed at a very local scale and all results are strictly observational and descriptive, additional studies should be performed at a larger spatial scale and buttressed with controlled laboratory and field enclosures experiments to identify the causal factors responsible for the identified patterns.

Thesis (MSc)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: This study aimed to understand how Dukuduku Forest in Kwa-Zulu Natal has changed from 1960 to 2008 and whether the change in political regimes, during and post apartheid eras might have contributed to changes in forest extent. To achieve the aims, the following analyses were made: - Qualitative and quantitative spatial analyses of forest change; - Analyses of the correspondence of change with political changes in the country; - Assessment of perception of people living in the Dukuduku forest area. The Dukuduku land cover was mapped from aerial photos using ArcGIS 9.3 to determine whether or not there has been a significant change in the area from 1960-2008, in response to resource use pressures and to come up with the strategic sustainable management plan from the results found. Five aerial photographs were used to determine the changes in land cover from the year: 1960, 1970, 1992, 2005 and 2008. The Land cover types were classified into four classes, Indigenous Forests, Plantation Forests, Water Bodies and Other (open areas, cultivated land, and all the human disturbed and transformed land). The percentage of cover per class was compared across the years to determine overall change in land cover and the rate of change per year was also calculated. The results from the study showed that: - Natural Forest increased by 11% (700 ha), at the rate of 20.56 hectares per year between 1960 and 1992, which is the apartheid era. Between 1992 and 2008, the democratic era, the forest decreased by 34.4% (2472.31ha), at the rate of 168 hectares per year. - The Dukuduku forest community gains resources (timber and grass for construction, art, firewood, medicinal plants, grazing of livestock and food) from the forest. The people are willing to contribute in protecting the forest only if the governing authorities would include them in decisions made, as the NFA demands Participatory Forest Management, but which does not currently exist in Dukuduku. / AFRIKAANSE OPSOMMING: Hierdie studie ondersoek die verandering van die Dukuduku woud in Kwa-Zulu Natal vanaf 1960 tot 2008, en vernaamlik of die verandering in politieke regimes tydens en in die postapartheid eras tot verandering bygedra het in die woud se vorm. Om hierdie doelwitte te breik is die volgende analises gedoen: - Kwalitatiewe en kwantitatiewe ruimtelike analises van woudverandering; - Analises van die korrelasie tussen hierdie fisiese omgewingsverandering en politieke verandering in die land; - Analise van die persepsie van mense wat in die Dukuduku woudgebied woon. Die Dukuduku gronddekking is gekarteer met behulp van lugfotos, waarvoor ArcGIS 9.3 gebruik is om te bepaal of daar noemenswaardige verandering in die gebied plaasgevind het van 1960 tot 2008, in reaksie op hulpbrongebruike, en om ‘n volhoubare bestuursplan gestel voor wat op die bevindinge gebaseer is. Vyf lugfotos is gebruik om verandering in gronddekking te bepaal vir die jare: 1960, 1970, 1992, 2005 en 2008. Die Gronddekking tipes is geklassifiseer in vier klasse naamlik Inheemse Woude, Plantasiebosse, Waterliggame en Ander (oop gebiede, landerye en al die mens-versteurde en getransfomeerde gebiede). Die persentasie van elke dekkingsklas is oor die jare vergelyk om die verandering in algehele grond-dekking te bepaal, en die tempo van verandering is ook bepaal, asook die tempo van verandering. Die resultate van die studie wys dat: - Die natuurlike woud toegeneem het met 11% (700 ha), teen ‘n tempo van 20.56 hektaar per jaar tussen 1960 en 1992, tgedurende die apartheidsera. Tussen 1992 en 2008, die demokratiese era, het die woude verminder met 34.4% (2472.31 ha), teen ‘n tempo van 168 hektaar per jaar. - Die gemeenskap wat in die Dukuduku woud woon verkry hulpbronne van die woud (hout en gras vir konstruksie, kuns, brandhout, medisinale plante, weiding vir vee, en voedsel). Die mense is gewillig om by te dra tot beskerming van die woud indien die owerhede hulle sou betrek in besluite wat geneem word, veral omdat die nasionale Wet op Bosse voorsiening maak vir Deelnemende Bosbestuur, wat tans nie by Dukuduku gebeur nie.

The northern bobwhite (Colinus virginianus) population decline is a severe, rangewide phenomenon beginning >150 years ago and continuing today. In this investigation, I: 1. document the timeline of bobwhite population decline and unintended genetic consequences of attempted remedies, 2) develop a model useful for predicting possible locations of potentially sustainable bobwhite populations in semiarid rangeland in Texas and Oklahoma, and 3) examine the relationship between population monitoring data and meteorological factors. While breeding season call counts of male bobwhite have been used for >70 years to provide estimates of fall populations for hunting, most studies of call counts have focused on mathematics and statistical accuracy of the count, largely overlooking the influence of meteorological factors on call counts. Here, I present the results of >4,400 individual point counts and examine their relationship with meteorological variables recorded at each stop. Humidity was positively correlated with the number of birds recorded (ρ = 0.275, p < 0.001) and temperature was negatively correlated (ρ = -0.252, p < 0.001). The number of birds recorded was significantly higher in wet years than in drought years. There was no significant correlation between wind velocity and number of birds recorded. These results suggest that, while weather does influence call counts and efforts should be made to record meteorological conditions when collecting call count data, the influence of weather may not easily factor into the analysis. These results also provide another line of evidence for decreased breeding behavior during high temperatures. With the increased focus on bobwhite habitat management on a regional scale, there is a need for reliable methods to identify potential bobwhite habitat. To identify bobwhite habitat in semiarid rangeland, I performed classification of LANDSAT scenes of Clay County, Texas from July and December 2015. Stands of mature little bluestem provide excellent bobwhite nesting cover and could be identified using LANDSAT imagery. I scored habitat by type, compared these scores with the results of breeding season call counts from 2014 and 2015 and found significant correlation. When used in combination with other landscape data, this approach can provide a regional context to inform conservation and management decisions.