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Monitoring von Membranen und membrangebundenen Dehydrogenasen in Essigsäurebakterien / Monitoring of membranes and membrane-bound dehydrogenases in acetic acid bacteriaKokoschka, Sebastian 21 October 2013 (has links)
No description available.
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Extended Scope and Understanding of Zinc-Dependent Alcohol Dehydrogenases for Reduction of Cyclic α-DiketonesStark, Frances, Hoffmann, Aaron, Ihle, Nadine, Loderer, Christoph, Ansorge-Schumacher, Marion B. 19 August 2024 (has links)
Alcohol dehydrogenases (ADH) are important tools for generating chiral α-hydroxyketones. Previously, only the ADH of Thauera aromatica was known to convert cyclic α-diketones with appropriate preference. Here, we extend the spectrum of suitable enzymes by three alcohol dehydrogenases from Citrifermentans bemidjiense (CibADH), Deferrisoma camini (DecADH), and Thauera phenylacetica (ThpADH). Of these, DecADH is characterized by very high thermostability; CibADH and ThpADH convert α-halogenated cyclohexanones with increased activity. Otherwise, however, the substrate spectrum of all four ADHs is highly conserved. Structural considerations led to the conclusion that conversion of diketones requires not only the expansion of the active site into a large binding pocket, but also the circumferential modification of almost all amino acid residues that form the first shell of the binding pocket. The constellation appears to be overall highly specific for the relative positioning of the carbonyl functions and the size of the C-ring.
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Identifizierung und Charakterisierung von Genen und korrespondierenden Genprodukten aus Metagenombanken, die Butanol-Dehydrogenase-Aktivität oder proteolytische Aktivität vermitteln / Identification and characterization of genes and encoding gene products of metagenomic libraries conferring butanol dehydrogenase activity or proteolytic activityWaschkowitz, Tanja 03 May 2006 (has links)
No description available.
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Untersuchung der Stressantwort von <i>Picrophilus torridus</i> mittels 2D-Gelelektrophorese und Charakterisierung ausgewählter Dehydrogenase / Stress response in <i>Picrophilus torridus</i> and characterization of different dehydrogenasesThürmer, Andrea 23 January 2008 (has links)
No description available.
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