Thesis (doctoral)--Karolinska Institutet, Stockholm, 1982. / Extra t.p. with thesis statement inserted. Includes bibliographical references.

Thesis (doctoral)--Karolinska Institutet, Stockholm, 1982. / Extra t.p. with thesis statement inserted. Includes bibliographical references.

Akademisk avhandling - Göteborg. / Added title page, with thesis note, inserted. Includes bibliographical references (p. 63-68).

Thesis--Bergen. / Includes 5 articles by the author reprinted from the Journal of periodontology and Acta odontologica Scandinavica. Includes bibliographical references.

Thesis--Bergen. / Includes 5 articles by the author reprinted from the Journal of periodontology and Acta odontologica Scandinavica. Includes bibliographical references.

Thesis (M.Sc.D.)--Boston University. School of Graduate Dentistry, 1969. / Bibliography, tables included. / An investigation of the fluoride concentration of cementum of rat molars was performed utilizing sixty Sprague-Dawley rats, averaging eighty grams, thirty-one to thirty-six days of age at the onset of the project. The experimental period was from twenty-two to thirty-five days. The rats were randomly placed into seven groups and were fed Purina Laboratory chow and tap water, to which had been added either none, 50, or 100 ppm fluoride. At the conclusion of the study the first and second maxillary and mandibular molars were extracted and the apical one millimeter of each root was collected for analysis. The samples were ashed and then dissolved in l ml of 0.5 M HC10 [subscript 4] and 4 ml of 0.5 M Na Citrate. The fluoride concentration of the samples was obtained by use of the Ionalyzer (Orion Research, Inc., Mode1 401) with its component specific fluoride ion activity electrode and essential reference electrode and standardizing solutions. The results were statistically significant (p <.00l) and the mean fluoride values ranged from 500 ppm to 2,100 ppm for the controls and from 3,900 ppm to 6,500 ppm for the experimental rats ingesting 100 ppm fluoride in their drinking water. The experimental design and significance of the results are discussed.

Thesis (M.S.)--University of Michigan, 2004. / Includes bibliographical references.

Thesis (M.S.)--University of Michigan, 1960.

Dental cementum is a mineralized tissue that coats the root of a tooth and anchors it into the alveolar socket via the periodontal ligament. Cementum is continuously deposited and mineralized throughout the life of a tooth, preserving the complex developmental processes of root formation in optically distinct histologic layers (Hillson, 1996; 2005). Analysis of these features has several anthropological applications, specifically the analysis of cementum increments and their utility in estimating age and season at death in humans for establishing a more specific post-mortem interval (PMI). Wedel (2007) conducted a pilot study to determine whether dental cementum increment analysis can be used to establish season of death in humans. Wedel (2007) hypothesized that by identifying the timing of the transition between the bands of arrested development and the bands of increased deposition, dental cementum increment analysis can be used to identify the season at death in humans. It was demonstrated that cementum increment analysis is 99% accurate in estimating whether an individual died in either a fall/winter or spring/summer season. The purpose of the present study was to estimate whether cementum bands could accurately be assigned to fall/winter, spring/summer seasons based on their optical properties as being light or dark. A total of 143 teeth of known age and known extraction date drawn from the Boston University Collection and the Antioquia Modern Skeletal Reference Collection in Medellín, Colombia were analyzed using recent protocols for preparation, sectioning, and observing increment lines in cementum. Dental cementum increment analysis was found to be between 61.54% and 71.15% successful in accurately correlating the nature of the last cementum increment to season at death using a combination of image evaluation and through focus evaluation methods on two distinct geographic samples. A through focus evaluation was found to be more successful at identifying the last band formed and is recommended for microscopic analyses estimating season at death using dental cementum increments. Geographic origin did not have a significant influence over the accuracy of the method to estimate season at death, however the results of this study suggest that teeth extracted from cadavers versus living individuals may have some influence on the accuracy of cementum increments to estimate season at death. No significant influence of sex, age, or tooth type on the identification and correlation of the last band formed were detected in this study. An interobserver analysis using digital images of a randomly selected sample of 45 sections found that interobserver agreement on the nature of the last band formed occurred in only 28.8% of the sample. Inconsistencies in the nature of the last band formed between multiple sections prepared from the same tooth were observed, which calls into question the validity of using cementum increments to estimate season at death. The potential limitations for the reliability of using dental cementum increment analysis to determine season at death in humans include the lack of a standardized method for preparing adequate sections for viewing cementum increments, and the subjectivity of identifying the last band in a given section. It is concluded that if the validity and reliability of dental cementum increment analysis as a method for age estimation can be established and configured to meet the criteria of the Daubert Standard, specifically in the adoption of a standardized protocol of analysis, then the validity of using this method for estimating season at death can be further considered.

Made available in DSpace on 2014-06-11T19:33:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-02-15Bitstream added on 2014-06-13T20:05:27Z : No. of bitstreams: 1 nassar_ca_dr_arafo.pdf: 644620 bytes, checksum: c8f926b8b07b13ddf9cd03079f1d6f41 (MD5) / Tacrolimus (FK506) é uma droga imunossupressora usada em transplantes de órgãos e também como terapia alternativa em pacientes com rejeição refratária de enxertos ou intolerância à ciclosporina A (CsA), sem os efeitos adversos atribuídos freqüentemente à CsA. Atualmente não há estudos que explorem os efeitos da administração de FK506 por longos períodos sobre o periodonto. Ë sugerido também que terapias imunossupressivas, principalmente com FK506, possam ser um importante fator no desenvolvimento de Diabetes mellitus pós-transplantes (PTDM). Existem alguns estudos explorando os efeitos do FK506 por longos períodos no desenvolvimento da PTDM em protocolos animais. Assim os objetivos do estudo foram avaliar os efeitos da terapia com FK506 por longos períodos sobre os tecidos gengivais, metabolismo do osso alveolar e do cemento de ratos, bem como os efeitos sobre os níveis glicêmicos. Com relação ao metabolismo ósseo, houve uma tendência de diminuição, estatisticamente significante, dos níveis de ALP em todos os períodos tratados com FK506, sendo que com os níveis de cálcio apenas um aumento em 240 dias de tratamento, quando comparados com os grupos controles. Assim, nós podemos concluir que o FK506 em longos períodos pode levar os efeitos deletérios sobre os tecidos gengivais e pode ser dependente da idade, mas não causaria efeitos negativos sobre o osso alveolar e nem ao cemento. E ainda em relação aos níveis séricos de glicemia, os efeitos negativos da terapia com FK506 também são dependentes do tempo. / Tacrolimus (FK506) is an immunosuppressive drug in organ transplantation and it is also an alternative therapy used for transplant patients with either refractory graft rejection or intolerant to cyclosporine A (CsA), without the adverse effects frequently attributed to CsA therapy. Nevertheless there are not studies exploring the effects of long-term FK506 therapy on periodontium. It has been suggested that immunosuppressive therapy, mainly FK506, may be an important factor in the development of post-transplantation diabetes mellitus (PTDM). There is a lack of studies exploring the effects of a long-term FK506 on PTDM in animal's protocols. The objectives of the present study were to evaluate the effects of long-term therapy with FK506 on the gingival tissue, alveolar bone metabolism and cementum in rats and the deleterious glycemic effects of long-term therapy with FK506. There was a tendency of increase of serum glycemia level in the initial periods (60 and 120 days). Within the limits of this experimental study, it can be concluded that the deleterious gingival effects of FK506 administration may be time and age related side effects and that have not the negative effects of FK506 administration long-term on the alveolar bone and cementum. It can be concluded that the deleterious glycemia effects of FK506 therapy may be time related side effects.