Resumo: A alface é a hortaliça folhosa de maior consumo no Brasil, no entanto a dificuldade em produzi-la vem aumentando, principalmente pela infestação das áreas de produção por Bremia lactucae, sendo necessário o monitoramento anual para utilização ou desenvolvimento de cultivares resistentes. Diante do exposto, os objetivos do presente trabalho foram: identificação de raças de B. lactucae no Estado de São Paulo, durante os anos de 2008, 2009 e 2010 e obtenção de linhagens de alface crespa resistentes às raças SPBl:01, SPBl:02, SPBl:03, SPBl:04, SPBl:05, SPBl:06 e SPBl:07. Na primeira fase do estudo foram coletadas nos meses de agosto, setembro e outubro de 2008; agosto e setembro de 2009; e junho e julho de 2010 folhas de alface com esporângios de B. lactucae nos principais municípios produtores de alface do Estado de São Paulo. Após a multiplicação dos esporângios na cultivar suscetível Solaris, com posterior inoculação nas diferenciadoras, realizaram-se as avaliações, no mesmo dia do aparecimento da primeira esporulação na cultivar suscetível Cobham Green (DM 0). Na segunda fase do estudo realizou-se o cruzamento entre a cultivar Argeles e a linhagem JAB 4-13-7, bem como autofecundações, até a obtenção da geração F3, quando realizou-se o teste de resistência. Os dados permitiram concluir que: três novos códigos "Sextet" para Bremia lactucae ocorreram no Estado de São Paulo: 63/63/33/00, 63/63/02/00 e 63/31/03/00, denominados raças SPBl:05, SPBl:06 e SPBl:07, respectivamente; recomenda-se a utilização dos genes R-17, R-18 e R-38 como fontes de resistência ao míldio para o desenvolvimento de cultivares no Estado de São Paulo; e 19 progênies resistentes à B. lactucae, raças SPBl:01, SPBl:02, SPBl:03, SPBl:04, SPBl:05, SPBl:06 e SPBl:07, apresentaramse homozigotas, e podem ser avançadas e avaliadas no processo de seleção / Abstract: Lettuce is the most consumed leafy vegetable in Brazil. Its production, nonetheless, is meeting with increasing difficulties mainly due to the infestation of producing areas with Bremia lactucae. Thus, the annual monitoring of B. lactucae races becomes of essential importance to use or development of resistant cultivars. The objectives of this work were identification of new races of B. lactucae occurring during the years of 2008, 2009 and 2010 in the state of São Paulo, Brazil, and development of resistant lettuce cultivars to races SPBl:01, SPBl:02, SPBl:03, SPBl:04, SPBl:05, SPBl:06 and SPBl:07. In the first phase of experiment in August, September, and October of 2008; August and September of 2009; and June and July of 2010 lettuce leaf samples containing B. lactucae sporangia were collected in some of the lettuce producing areas of the state of São Paulo. After a multiplication procedure in which the susceptible lettuce cultivar 'Solaris' was used, the B. lactucae sporangia were inoculated in differentiating lettuce cultivars. Evaluations of the plants response were performed on the same day when plants of the susceptible Cobham Green (DM 0) cultivar showed signs of the first sporulation. In the second phase of experiment made the crossing between Argeles cultivar and JAB 4-13-7 line, and self pollination, to obtain the F3 generation, when made the resistant test. The data have identified: three new codes "Sextet" in the state of São Paulo: 63/63/33/00, 63/63/02/00 e 63/31/03/00. SPBl:05, SPBl:06 and SPBl:07 were the denomination proposed to races, respectively. The genes R-17, R-18, and R-38 are suggested to be used as source of resistance in the lettuce cultivars to state of São Paulo; and 19 resistant progenies to B. lactuae, races SPBl:01, SPBl:02, SPBl:03, SPBl:04, SPBl:05, SPBl:06 and SPBl:07, shown homozygous, and they can be advanced and evaluated in the selection process / Orientadora: Leila Trevizan Braz / Coorientador: Tagli Dalpian / Coorientadora: Margarete Camargo / Banca: Pablo Forlan Vargas / Banca: Érika Auxiliadora Giacheto Scalopp / Banca: Rita de Cássia Panizzi / Banca: Joaquim Adelino de Azevedo Filho / Doutor

Diplomová práce se zabývá problematikou hodnocení bonity klienta v české komerční pojišťovně. Skládá se ze dvou hlavních logických celků ? přípravy teoretického modelu bonity klienta a jeho praktického ověření na reálných datech jedné české pojišťovny. Příprava modelu bonity klienta se přidržuje postupu popsaného v metodice CRISP-DM. Postupně jsou prozkoumány současné způsoby sledování bonity klientů v českém bankovním i nebankovním sektoru a je rozebrán způsob určování bonity klienta v amerických pojišťovnách. Následuje samotné sestavování modelu bonity klienta v pojišťovně. Nejdříve jsou nalezeny oblasti ke sledování a z těchto oblastí jsou vybrány vhodné ukazatele bonity klienta. Přípravu modelu uzavírá nastavení vah u jednotlivých ukazatelů a popis sledovaných kategorií bonity klienta. Druhý logický celek se zabývá aplikací připraveného modelu bonity klienta v praxi. Popsána je fyzická architektura řešení, příprava datové základny, použitá skóringová aplikace a převedení modelu bonity klienta do této aplikace. Dalšími popsanými kroky jsou testování modelu na vzorku dat a na kompletním portfoliu klientů spolupracující pojišťovny. Výsledky jsou analyzovány a zobrazeny v grafech. Poté jsou obdržené výsledky porovnávány s očekávanými výsledky. Diplomová práce končí diskuzí k využití bonity klienta v reálných procesech pojišťovny.

This study was initiated to investigate a possible strategy to alter an enzyme deficiency in a mouse model. The enzyme investigated is a multifunctional nucleoside kinase from Drosophila melanogaster (Dm-dNK). This enzyme has special features in that it has higher enzymatic activity than any other known nucleoside kinases and still has similar substrate specificity as the human nucleoside kinases. The deficiency where the Dm-dNK transgenic mice model will be used is a TK2 deficient model with severe phenotype caused by mitochondrial DNA depletion. The Dm-dNK transgenic mice model will be used as a way to rescue the TK2 deficient mice. The results from the present study show that Dm-dNK expression in mice results in a substantial increase of thymidine phosphorylation in several investigated tissues. The mice were otherwise normal as judged by life span, weight and behavior. The mitochondrial DNA was also detected at normal levels. In conclusion, the Dm-dNK mouse model is promising as a way to rescue the severe phenotype of the TK2 deficient mice.

This thesis presents different Internet Protocol (IP) multicasting design models and together with their implementation methods in OPNET Modeler 14.5. The models presented in this report are designed for handling various types of advanced multimedia applications and services when using the multicast communication paradigm. The architectural models presented in this thesis are independent of the routing protocols used as well as of the underlying networking environment. Several exiting challenges related to high bandwidth requirements, low network delay, and jitter are discussed together with these design models, and are simulated using the OPNET Modeler. The emerging demands for new multicast capable applications, for both real-time and non real-time traffic are considered together in this report in order to better evaluate multicast traffic requirements. Efficient routing methods, group management policies, and shortest path algorithms are also discussed in the proposed models. The pros and cons of IP multicasting are described using the Protocol Independent Multicast Sparse Mode (PIM-SM) method, in the highlight of the proposed design models. Several results related to link utilization, end-to-end delay, packet loss, PIM control traffic, Internet Group Management Protocol (IGMP), and network convergence are obtained by using already available statistical methods and modules from OPNET Modeler. Our major contribution with this thesis work relates to components associated with network convergence, routing delay, and processing delay. These components are illustrated in this thesis using various OPNET metrics. Moreover, several issues related to protocol scalability, supportability, performance optimization and efficiency are also presented by using OPNET’s build in reports, i.e., executive flow and survivability analysis reports. / 0735609277

The Optical Spectrograph and InfraRed Imaging System (OSIRIS) satellite instrument spearheaded by a team of researchers at the University of Saskatchewan has provided scientists with 13 years of information about the state of the atmosphere. The success of the mission has motivated further development of the technology in a next generation instrument called the Canadian Atmospheric Tomography System (CATS) to provide better spatial and spectral resolution through a successive satellite mission. This work details the development of a proof-of-concept prototype built to test the validity of the CATS optical design. This thesis project utilized the developmental model built for the OSIRIS mission. The major modification made to the instrument replaced the optical element that defines the instrument’s field of view. This new development transformed the original single line of sight utilized by the satellite based OSIRIS instrument into three separate fields of view, which increased the imaging capabilities of the instrument. The new system has improved spatial resolution by collecting spectral information from three separate regions in the atmosphere in a single exposure, as opposed to the single region imaged by the original system. The newly developed prototype was tested on the platform of a stratospheric balloon. This test platform offered the capabilities to make limb scattered measurements similar to those that are made by a satellite based instrument. However, from the balloon geometry, the instrument required an additional pointing system to redirect the line of sight over stratospheric tangent altitudes. The design and test of this pointing system is also detailed in this work.

The development of remote sensing satellite-borne instrumentation for the study of the Earth’s atmosphere has provided an immense increase in our understanding of atmospheric trends and processes. The Canadian built OSIRIS satellite instrument uses the limb scatter technique to measure scattered sunlight for the retrieval of vertical profiles of atmospheric species such as ozone. Recently, the next generation instrument, CATS, based on the OSIRIS design, is under development to continue OSIRIS measurements into the future. One key optical design change for CATS is the ability to measure simultaneously over multiple fields of view. However, this new optical design concept needs to be tested and evaluated. To achieve this, a prototype slit plate was installed into the preflight developmental version of OSIRIS, called OSIRIS-DM, for testing in the laboratory and on a stratospheric balloon. In this thesis work, an evaluation of the performance of this multi-slit instrument was undertaken through laboratory calibrations and limb scatter measurement collection. The calibration process includes a wavelength registration, a spectral point spread function analysis, a relative calibration and an absolute calibration, all performed with laboratory equipment. Along with laboratory calibrations, this thesis work involved preparation for the stratospheric balloon mission including the development of a flight ready electronic control and communication system to operate OSIRIS-DM during the mission. The upgraded instrument was launched on September 19, 2014, and ascended to a stable float altitude of 36.5 km. The collected flight measurements were used to evaluate the calibrations and general instrument performance. Overall, the laboratory calibrations proved to be sufficiently accurate and the measurement collection produced multiple spectra that may be used for atmospheric analysis in the future. These results show that the multi-slit design of the slit plate produces an instrument that can be reliably calibrated and implemented for limb scatter measurement collection.

abstract: The field of Data Mining is widely recognized and accepted for its applications in many business problems to guide decision-making processes based on data. However, in recent times, the scope of these problems has swollen and the methods are under scrutiny for applicability and relevance to real-world circumstances. At the crossroads of innovation and standards, it is important to examine and understand whether the current theoretical methods for industrial applications (which include KDD, SEMMA and CRISP-DM) encompass all possible scenarios that could arise in practical situations. Do the methods require changes or enhancements? As part of the thesis I study the current methods and delineate the ideas of these methods and illuminate their shortcomings which posed challenges during practical implementation. Based on the experiments conducted and the research carried out, I propose an approach which illustrates the business problems with higher accuracy and provides a broader view of the process. It is then applied to different case studies highlighting the different aspects to this approach. / Dissertation/Thesis / M.S. Computer Science 2012

My diploma thesis deals with the issue of data mining and its use in the commercial sphere. The aim of my work was first to assemble knowledge from data mining and then use it on particular data. In the first part I gather the theoretical information about data mining. I focused on definition, methods of data mining, algorithms and of course on the most frequent usage. The second part consists of the practical application of acquired knowledge on real-world date from mobile telecommunications.

Effective device management is not trivial due to a variety of devices and software. To keep costs to minimum companies must effectively utilize a unified solution for device management. This research investigated Funambol’s implementation of Open Mobile Alliance Device Management (OMA DM) which is the most popular device management solution. Interviews were used to set experiments and create realistic test cases. A set of devices and a collection of Funambol software were used to create device management sessions. All of the sessions were recorded, analysed, manipulated and resent to identify efficient ways of device management. Additionally, an influence of compression and buffer-like mechanisms were checked. Methods and guidelines are provided for efficient use of OMA DM as well as a reliable analysis of OMA DM Sessions under various conditions. It was found that for most data it is best to use a built-in transport protocol compressor. Hypertext Transfer Protocol’s (HTTP) deflate with a combination of client-side buffering-like mechanism at a client side performed best at most cases. Funambol’s implementation of the Binary Extensible Markup Language (WBXML), in most cases, performed very badly, even though it was designed specifically to compress OMA DM Session messages. It was found that for an efficient use of OMA DM a proper software option set (e.g. forced use of compression) may be sufficient.

MSc (Chemistry) / Department of Chemistry / Bridelia micrantha (Hochst) Baill (B. micrantha) is a South African medicinal plant used by traditional healers in the treatment of different human ailments including diabetes, gastrointestinal ailments, joint aches, cough, conjunctivitis, skin problems and malaria. Previous studies have demonstrated the antidiabetic activities of B. micrantha crude extracts in in vivo studies. However, there are no studies on the compounds responsible for the antidiabetic activity of the plant. The purpose of this study was to isolate and characterize the antidiabetic constituents from B. micrantha. Materials and methods Crude methanolic extracts of root, stem and leaf were investigated using in vitro antidiabetic enzyme assays. Antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The isolation of the antidiabetic constituents was carried out using column chromatography on silica gel. Purification of the active fraction was carried out using preparative thin layer chromatography (pTLC). Structure elucidation of the compound was carried out using Nuclear Magnetic Resonance (NMR) spectroscopy and by comparison with literature. Results The results obtained in this study indicated that root, stem and leaf extracts exhibited high inhibition activity against α-glucosidase (98.52, 98.62 and 81.62% respectively). A moderate inhibition against α-amylase enzyme was observed for root (65.62%) and stem (61.86%) extracts. Leaf fraction LFR5 exhibited a high inhibition activity of 96.19% against α-glucosidase. Moreover, the isolated compound showed 96.74% inhibition against α-glucosidase. DPPH results revealed that antioxidant activity of crude extracts was not significantly different and they were concentration-dependent. Reducing power results revealed that stem (119.31 μg/mL) extract had higher activities compared to root (125.17 μg/mL) and leaf (291.88 μg/mL) extracts. Conclusion Quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside was successfully isolated from B. micrantha leaves. Furthermore, quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside demonstrated the ability to inhibit significantly the carbohydrate hydrolysing enzyme α-glucosidase and therefore validate the ethnomedicinal use of B. micrantha in the management of diabetes / Bridelia micrantha (Hochst) Baill (B. micrantha) is a South African medicinal plant used by traditional healers in the treatment of different human ailments including diabetes, gastrointestinal ailments, joint aches, cough, conjunctivitis, skin problems and malaria. Previous studies have demonstrated the antidiabetic activities of B. micrantha crude extracts in in vivo studies. However, there are no studies on the compounds responsible for the antidiabetic activity of the plant. The purpose of this study was to isolate and characterize the antidiabetic constituents from B. micrantha. Materials and methods Crude methanolic extracts of root, stem and leaf were investigated using in vitro antidiabetic enzyme assays. Antioxidant activities were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The isolation of the antidiabetic constituents was carried out using column chromatography on silica gel. Purification of the active fraction was carried out using preparative thin layer chromatography (pTLC). Structure elucidation of the compound was carried out using Nuclear Magnetic Resonance (NMR) spectroscopy and by comparison with literature. Results The results obtained in this study indicated that root, stem and leaf extracts exhibited high inhibition activity against α-glucosidase (98.52, 98.62 and 81.62% respectively). A moderate inhibition against α-amylase enzyme was observed for root (65.62%) and stem (61.86%) extracts. Leaf fraction LFR5 exhibited a high inhibition activity of 96.19% against α-glucosidase. Moreover, the isolated compound showed 96.74% inhibition against α-glucosidase. DPPH results revealed that antioxidant activity of crude extracts was not significantly different and they were concentration-dependent. Reducing power results revealed that stem (119.31 μg/mL) extract had higher activities compared to root (125.17 μg/mL) and leaf (291.88 μg/mL) extracts. Conclusion Quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside was successfully isolated from B. micrantha leaves. Furthermore, quercetin-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnoside demonstrated the ability to inhibit significantly the carbohydrate hydrolysing enzyme α-glucosidase and therefore validate the ethnomedicinal use of B. micrantha in the management of diabetes.