Research in the field of drug dependence and recovery emphasises the need for more understanding of the concept of recovery from dependent drug use. This study explored the ways in which dependent drug users recover from drug dependency in a Christian faith-based agency in Lagos, Nigeria. The strategy employed was a qualitative research design using a case study approach. Instruments for data collection were qualitative interviews and observation methods; agency records were also accessed for background information purposes. Three stages of the recovery process were explored: motivation for recovery, disengagement from drugs and maintenance of recovery. In each of these stages, psychological, socio-environmental and spiritual elements were identified as significant factors in the recovery process. The offer of treatment from a Christian faith-based agency seemed to be the most important factor in motivating informants to engage in treatment in the first place. At the disengagement stage, psychological and socio-environmental issues came to the fore, with a personal commitment to change and support from significant others including peers becoming important. Spiritual factors played a significant part at this time, however, including teaching and Bible reviews and prayers. Maintenance of recovery was found to be facilitated by psychological strategies such as positive self-talk and avoidance of triggers of drug dependency; by socio-environmental factors including supportive relationships; and by spiritual elements, which centred on the adoption of a Christian lifestyle. The findings conclude that although recovery from drug dependence is achieved through various routes, the most significant factor for the informants in this study was the spiritual intervention received.

No description available.

No description available.

Binding sites for substance P(1-7), SP(1-7) have been identified and characterized for the first time in crude membrane fraction from rat CNS using tritiated ([3H]) SP(1-7) as tracer. These putative receptors were investigated in relation to their affinity for tachykinins, opioid peptides and sigma receptor ligands. [3H]-SP(1-7) specifically binds to high affinity binding sites identified as receptor targets for the heptapeptide SP (1-7). Two distinct binding sites were observed in the spinal cord. One site is recognized by high affinity for SP(1-7) with a Kd of 0.5 nM, whereas the other site showed low affinity for the heptapeptide (Kd=12 nM). In the brain, the binding of SP(1-7) fitted a single site binding model with a Kd of 4.4 nM and a Ki of 4.2 nM. Further, using the spinal cord membranes the binding of [3H]-SP (1-7) was weakly displaced by SP and other N-terminal fragments thereof and no or negligible affinity was observed for ligands of the NK-1, NK-2 and NK-3 tachykinin receptors, C-terminal SP(5-11), Tyr-w-MIF-1 or the mu-opioid receptor antagonists naloxone and naloxonazine. On the other hand it was significantly displaced by endomorphin-2, DAMGO, and Try-MIF-1 and exhibit some affinity for MIF-1, ß-casomorphin and endomorphin-1. However, only endomorphin-2, DAMGO and Tyr-MIF-1 showed affinity in the close range of the native peptide SP(1-7). The affinity of endomorphin-2 for the spinal cord site was 10 times lower than that of SP(1-7) but more than 100 times higher than the affinity recorded for endomorphin-1. Tyr-MIF-1 but not Tyr-w-MIF-1 showed similar affinity as endomorphin-2 for SP(1-7) site. All peptides exhibiting high affinity at the SP(1-7) site, have a phenylalanine or a leucine residue in their C-terminal structure. Further, synthetic analogues of SP(1-7) were tested for their affinity for the SP(1-7) receptor in the rat spinal cord. An important finding here was that the receptor-ligand-interaction was favoured by the C-terminal region of SP(1-7). Residues at positions 5-7 appeared crucial for binding to the specific SP(1-7) site. The presence of the amidated Phe7 residue was extremely critical for binding to the SP(1-7) site.The analogue Gln5-Gln6-Phe7-NH2 was almost equipotent with the parent peptide in the SP (1-7) receptor binding assay. Furthermore, the SP(1-7)-amide potently and dose dependently reduced several signs of the reaction to morphine withdrawal and was significantly attenuated by the addition of the sigma agonist SK-10047. In conclusion, the work presented in this thesis has contributed the characterization of the properties of highly selective binding sites for SP(1-7) in the rat spinal cord and VTA. These sites appear to be distinct from the µ-opioid receptor or any of the known neurokinin receptors. The study further indicates that the SP(1-7)-amide mimics the effect of the nativ heptapeptide and that the mechanisms for its action involve a sigma receptor site.

No description available.

Tolerance to anesthetic drugs is mediated partially by homeostatic mechanisms that attempt to restore normal neural excitability. The BK-type Ca2+-activated K+ channel, encoded by the slo gene, plays an important role in this neural adaptation. In Drosophila, a single sedative dose of the organic solvent anesthetic benzyl alcohol induces dynamic spatiotemporal changes in histone H4 acetylation across the slo regulatory region and leads to slo induction and tolerance. Mutations ablating the expression of slo also block the acquisition of tolerance, whereas activating the expression of a slo transgene results in resistance to drug sedation. Moreover, artificially inducing histone acetylation with the histone deacetylase inhibitor causes similar acetylation changes, slo induction, and functional tolerance to the drug. Histone acetylation changes occur over two highly conserved non-coding DNA elements, 6b and 55b, of the slo control region. To investigate the function of these two elements, I generated individual knockout mutants by gene targeting. Both knockout alleles are backcrossed into the CS wild type background. The 6b element seems to repress slo induction after drug sedation, because the 6b knockout allele overreacts to the drug. Compared to the wild type, 6b knockout allele shows a much greater slo message induction after drug sedation, it also displays stronger enhancements in seizure susceptibility and following frequency. In addition, the 6b deletion causes a persistent tolerance for at least a month, while tolerance only lasts about 10 days in wild type flies. My investigation also indicates that the 55b element limits basal slo expression in muscle. Finally, to investigate if the particular histone acetylation spikes are required for drug-induced slo induction and tolerance, I tether the histone-modifying enzymes, HDAC or HAT, to the 6b and 55b DNA elements, respectively. I observe that the positioning of an HDAC on these two elements blocks drug-induced slo induction and the development of tolerance. Therefore, histone acetylation across slo control region is required for the activation of slo and the acquisition of tolerance. / text

The purpose of this study was to examine the relationship between history of parental substance abuse and substance use and psychiatric severity measures in a sample of treatment-seeking, pregnant women who meet DSM-IIIR criteria for lifetime opioid and cocaine abuse and/or dependence (N=260). The study examined whether a dose-response relationship was observed between number of parents affected by substance use problems (0, 1, and 2) and degree of women’s substance use and psychiatric severity. Results did not support a dose-response relationship between density of parental problems and substance use and psychiatric severity. However, some differences in severity were seen at different levels and types of parental density. Overall, the present study supports the need to further examine parental history of substance abuse as a potential risk factor for more severe substance use and psychiatric problems. Study findings have important implications for the screening and treatment of substance dependence during pregnancy.

This study examines the relationship between age of onset of drug use and later drug dependence, and that of age of onset of drug use and current offense type/severity. In addition, it investigates the relationship between mental disorders, drug dependence, and current offense type/severity. Data from years 2007 to 2010 of the Arrestee Drug Abuse Monitoring Program II (ADAM II) were used. The analyses included cross tabulation and chi square. The results indicated that early onset marijuana users (those who began using at age fourteen or younger) were more likely to develop drug dependence than late onset users of marijuana. In addition, early onset users of heroin and of methamphetamine were more likely to develop drug dependence than late onset users of those drugs. No significant relationships were found between early onset of any of the four drugs and offense type; however significant relationships were found between early onset of marijuana and of methamphetamine, and offense severity. Significant relationships were found between offense severity and mental disorders, but not between offense type and mental disorders. Significant relationships were found for both offense type and severity when cross tabulated with mental disorders and drug dependence. These results indicate that more research is needed on these topics. This is because the findings of the current study partially support what has been found in existing literature. A clearer understanding of the topics of the current study is needed in order to draw definite conclusions.

<p>Syftet med studien är att belysa hur barn i åldrarna tre till fem år tänker kring genus. Barnen fick lyssna på sagan ”Prinsessan Papperspåse” (Robert N. Munsch, 1980) och intervjuades sedan med utgångspunkt från sagan. Resultatet visar att barnen tänker relativt lika kring genus oberoende av kön och ålder. Både flickorna och pojkarna hade tydliga föreställningar om vad som är ett acceptabelt uppträdande av det motsatta könet. Framför allt hade de klara tankar om hur en flicka ska se ut om hon ska gifta sig. Att vara smutsig är för barnen inte acceptabelt, vare sig man är flicka eller pojke. Utan undantagsvis lade flickorna fokus på relationen mellan prinsessan och prinsen medan pojkarna lade fokus mer på draken och prinsen.</p>

No description available.