The Atomic Force Microscope (AFM) is an instrument that is capable of measuring intermolecular forces between single molecules. Multi-Parameter Force Spectroscopy (MPFS) is a technique that uses the AFM. MPFS enables the acquisition of force curves and thermal resonance of the system under investigation. This technique can shed light on the mechanical behavior at the molecular level. Improvements described herein have enhanced the sensitivity of MPFS over background noise. This investigation focuses on the mechanical and interfacial properties of three carbon nanostructures: long nanotubes, nanocoils, and nanoloops. Different types of adhesion are encountered, measured and discussed: friction, rupture, and peeling. The elastic modulus of long carbon nanotubes is calculated from frequency shifts when the system is put into tension. An elastica model is applied to the post-buckled carbon nanotubes, which enables the estimation of the static coefficient of friction on chemically modified surfaces. The compression of a nanocoil at large contact angles reveals that changes in oscillation amplitude do not occur from damping, but from adding stiffness into the systems measured herein. This result is counter to the assumptions of dynamic force spectroscopy. Finally, carbon nanoloops are brought into and out of contact with several different surfaces. The force curve and frequency response of the system shows the difference between rupture and peeling. The results presented herein lead to a better understanding of the mechanical and tribological properties of the carbon nanostructures.

This thesis deals with the radial forces acting on the impeller of a centrifugal pump. It focuses on the centrifugal pump type BETA 26. It addresses forces acting on the pump shaft. These forces are converted into the impeller. The most extensive part of this thesis is devoted to the experimental measurement and evaluation. The results are summarized and processed graphically.

In dieser Dissertation werden die Bindungseigenschaften von Rezeptor-Ligand-Komplexen mit Hilfe von Optischen Pinzetten untersucht. Aufgrund ihrer außerordentlichen Orts- (2nm) und Kraftauflösung (0,2pN) ist es möglich, diese spezifischen Interaktionen anhand einzelner Bindungsereignisse zu charakterisieren. Als Modellsysteme dienen die Wechselwirkungen zwischen den phosphorylierungsspezifischen, monoklonalen Antikörpern HPT-101 und HPT-104 und dem Morbus Alzheimer relevanten Tau-Peptid. Dieses pathogen veränderte Peptid wird krankheitsspezifisch an den Aminosäuren Threonin231 und Serin235 phosphoryliert, sodass die Detektion dieses Phosphorylierungsmusters mit Hilfe von monoklonalen Antikörpern eine mögliche Früherkennung der Alzheimer-Krankheit darstellt. Eine notwendige Voraussetzung dafür ist jedoch die exakte Kenntnis der Bindungsstellen des Liganden am Rezeptor. Ziel des ersten Teils dieser Arbeit ist es, das Epitop des monoklonalen Antikörpers HPT-101 zu bestimmen. Dazu werden mögliche bindungsrelevante Aminosäuren durch ein Alanin ausgetauscht (Alanin-Scan) und so insgesamt sieben neue Tau-Isoformen aus dem ursprünglichen doppelt-phosphorylierten Peptid Tau[pThr231/pSer235] hergestellt. Die jeweiligen Interaktionen zwischen den modifizierten Peptiden und dem Antikörper werden mit der dynamischen Kraftspektroskopie untersucht und mit Hilfe eines literaturbekannten Modells analysiert. Die sich daraus ergebenden Bindungsparameter (Lebensdauer der Bindung, charakteristische Bindungslänge, freie Aktivierungsenergie und Affinitätskonstante) werden zusammen mit den relativen Bindungshäufigkeiten erstmals genutzt, um Kriterien für essentielle, sekundäre und nicht-essentielle Aminosäuren im Tau-Peptid zu definieren. Bemerkenswerterweise existieren für insgesamt drei dieser Parameter (Bindungslebensdauer, Bindungslänge und Affinitätskonstante) scharfe Klassengrenzen, mit denen eine objektive Einteilung des Epitops von Antikörper HPT-101 möglich ist. Die erhaltenen Ergebnisse sind in überzeugender Weise im Einklang mit ELISA-Messungen zu diesem Antikörper-Peptid-Komplexen, sie liefern jedoch einen tieferen Einblick in die Natur einer spezifischen Bindung, da den kraftspektroskopischen Messungen auch die Bindungskinetik zugänglich ist. Das zweite Projekt der vorliegenden Dissertation etabliert eine Methodik, um die Datenvarianz in der Bestimmung der relativen Bindungshäufigkeit zu reduzieren. Anhand einer Kombination aus Fluoreszenz- und kraftspektroskopischen Messungen werden die Wechselwirkungen zwischen dem monoklonalen Antikörper HPT-104 und dem fluoreszenzmarkierten Peptid Tau[Fl-pThr231] untersucht. Es wird gezeigt, dass durch Vorsortieren der Peptid-beschichteten Kolloide, entsprechend ihrer Oberflächenbeladung, die Datenvarianz in den Bindungshäufigkeitsmessungen signifikant reduziert wird.

This thesis contains several contributions to the theoretical description and interpretation of biophysical single-molecule measurements: (i) For semiflexible polymers, we derive an efficient formulation of their local transverse dynamics in terms of a Generalized Langevin Equation. The elastic and frictional properties of the polymer are condensed into a memory kernel that is a function of the polymer\''s length and stiffness, the level of backbone tension, the position of the force probe along the polymer backbone and the boundary conditions at the polymer ends. At short times, the memory kernel attains a universal limiting form that depends neither on the polymer length nor on the boundary conditions; we obtain analytical results that accurately describe this regime. We discuss how to quickly and reliably evaluate the memory kernel for arbitrary times using a spectral decomposition method, and use an extensive body of numerical data to obtain analytical approximations to the memory kernel that cover the complementary long-time limit wherein polymer friction can be subsumed under a renormalized drag coefficient. (ii) Based on a systematic nonequilibrium treatment of an overdamped, one-dimensional stochastic escape process driven by external force, we develop a theory of Dynamic Force Spectroscopy (DFS) that generalizes previously available DFS theories to the high loading rates realized in novel experimental assays and in computer simulations. (iii) Extrapolating to future DFS experiments that may operate at far higher time resolution than presently achievable, we discuss the fast nonequilibrium relaxation of a semiflexible linker after bond rupture. Based on a rigorous theory of tension propagation in semiflexible polymers, we predict the relaxation of force within the force actuator, show that this relaxation is dominated by linker contraction, and demonstrate quantitative agreement of our predictions with experimental data obtained by a collaborating experimentalist group.

Since mankind found out that she is the reason to the recent global warming shehas, in some societies, begun a conversion of her society, from driven by fossil fuel tosustainable fuel. One of these is so called wave energy.CorPower Ocean's wave energy converter consist of a buoy, which through a wire,is connected to a shaft in the plant. This shaft drives two gas lled and pressurisedpistons, which stores the energy from the buoy's upward motion and returns it at thebuoy downward motion, aiming at evening the energy production over the cycle. Inthe wave energy converter there are several seals, between hydraulic uids, betweenhydraulic uids and the gas pistons as well as between the plant and the surroundingocean where the shaft exits the plant.To select the right kind of seals the range in the working temperature of the surroundinguids need to be known, as do the load of the waves on the shaft. This inorder to select seals of the right material and to construct a linear guide.The purpose of this thesis is to acquire the initial demands for the seal and linearguide. In other words make a thermal analysis of the surrounding environmentin order to select seals and an initial load analysis in order to construct a robustlinear guide.The result is that in order to control the temperature, a reservoir volume shouldbe added to the cylinders and a change in this volume gives the most eect on thetemperature and compression rate. Regarding the linear guides, slide rings on bothsides of the two outer seals should be enough. This thesis should be viewed as aninitial analysis of the sealing problem to be able to outline the demands on the sealsand linear guides related to the gas compartments. / Sedan manninskan ck reda pa att hon ar orsaken till den senaste tidens globalauppvarming har hon, i nagra samhallen, paborjat en omstallning av sitt samhalle,fran fossila branslen som drivmedel till langsiktigt hallbara drivmedel. Ett av dessaar sa kallad vagkraft.CorPower Oceans vagkraftverk bestar av en boj, som genom en vajer faster i endragstang i sjalva kraftverket. Denna dragstang driver tva gasfyllda och trycksattakolvar, som lagrar energin fran bojens uppgaende rorelse och aterger den vid bojensnedgande rorelse, med malet att dessa jamnar ut kraftproduktionen over cykeln. Iverket nns era tatningar, mellan olika hydraulvatskor, mellan hydraulvatskor ochgaskamrarna samt mellan verket och det omgivande havet dar dragstangen gar ut urverket.For att kunna valja ratt tatningar maste spannet i arbetstemperatur pa de omgivandeuiderna, samt lasten fran vagorna pa dragstangen, vara kanda. Detta foratt tatningar av ratt material ska kunna valjas och en linjarguide konstrueras.Detta arbete syftar till att ta fram de initiella kraven pa tating och linjarguide.Med andra ord gora en termisk analys av den omgivande miljon for att kunnavalja tatningar samt en inledande kraftanalys for att kunna konstruera en robustlinjarguide.Slutsatsen blir att for att styra temperaturen, bor en reservoirvolym laggas till cylindrarnaoch en andring av volymen pa denna ger storst eekt pa gasens temperaturoch kompressionsgrad. For linjarguidens del bor det ga bra med glidringar pa badasidor om de yttersta tatningarna. Det exakta valet av tatingslosning kommer Cor-Power Ocean dock att arbeta fram med ett lampligt foretag. Denna uppsats ska sessom en inledande analys av tatningsproblemet for att kunna ange huvuddragen avkraven pa tatningar och linjarguider relaterade till gascylindrarna

No description available.

This study investigated the implementation of a remaining range estimation algorithm for electrical vehicles, an essential feature to define a vehicle's reliability on the road. The implementation was made on an electrical motorcycle, comparing three models: a dynamic force based model, a power based model and a mass estimation model. The mass model estimated the mass with the help of a RLS algorithm and is a combination of the force based model and the power model. It investigates the possibility to further increase the accuracy of a range estimation algorithm by estimating the total mass of the vehicle over a driving session. On top of these models, two kinds of prediction methods for future consumption were evaluated: the average-past prediction and the home-intention prediction. Both models uses past data to predict the future, but the home-intention prediction is a suggested method to further improve the classic average-past method, where the beginning and end of the vehicle's driving sessions is assumed to be the same location. Tests were executed for the models on an electrical motorcycle provided by the company CAKE. A test equipment were put on the motorcycle, consisting of microprocessors and sensors, used for computation and collection of data. With this equipment, experiments were performed on three test routes with different conditions, comparing the models’ accuracies. The results showed that the Power Model, even with its lower complexity performed best overall, while the Force Model showed mixed results. Depending on the prediction method the Force Model performed either at the top or at the bottom. When the results were analyzed, this behavior seem to be the result of insufficient/faulty hardware which were essential for the average-past prediction to achieve proper results. The Force Model using home-intention prediction consistently performed better, as long as its prediction was correct. The Mass Model was executed offline and were then used to simulate the effect it could have had online. This showed promising result, suggesting improved accuracy if implemented online, but which in this thesis is left as a suggestion of improvement for future work. / Den här studien utforskade implementationen av en algoritm för att estimatera kvarstående räckvidd för ett elektriskt fordon, som är en viktigt funktionalitet för att utvärdera ett fordons pålitlighet på vägen. Implementeringen gjordes på en elektrisk motorcykel på tre modeller: en kraftbaserad-, en effektbaserad- och en massestimeringsmodell. Massestimeringsmodellen estimerar fordonets massa med hjälp av en RLS algoritm och är en kombination av kraft- och effektmodellen. Den utforskar möjligheten att förbättra räckviddsestimeringen ytterligare genom att kunna estimera den totala vikten av fordonet under körningen. På dessa modeller så utvärderades två typer av prediktionsmetoder för att förutspå framtida energiförbrukning: genomsnittliga-datametoden, en metod som använder genomsnittlig data i dåtid, och hem-avsiktsmetoden, en metod som förutspår förarens avsikt att åka hem. Båda modellerna använder gammal data för att förutspå framtiden, men hem-avsiktsmetoden är en föreslagen metod för att ytterligare förbättra den klassiska genomsnittliga-passerade metoden, där början och slutet av körningen antas vara samma position. Test utfördes för modellerna på en elektrisk motorcykel från företaget CAKE. En testutrustning monterades på motorcykeln som består av mikroprocessorer och sensorer och användes för samla och bearbeta data. Med denna utrustning genomfördes experiment på tre olika rutter som hade olika förutsättningar där modellerna träffsäkerhet sedan jämfördes. Resultatet visade på att Effektmodellen, även då den har en lägre nivå av komplexitet, faktiskt presterade generellt sätt bäst, medans Kraftmodellen visade på blandat resultat. Beroende på prediktionsmetod som användes så presterade Kraftmoddel antingen i toppen eller botten. När resultatet analyserades så verkar detta beteende bero på otillräcklig/problematisk hårdvara som var avgörande för den genomsnittliga-passerade metoden. Kraftmodellen tillsammans med hem-avsiktsmetoden ökade prestandan konsekvent så länge som förutsägelsen var korrekt. Massmodellen utfördes offline och detta resultat användes sedan för att simulera massmodellens påverkan på estimering online. Detta visade på lovande resultat och visar på att ifall den metod kördes online så skulle pricksäkerheten kunna ökas, men är något som inte utförs i detta arbete utan lämnas som ett förslag på förbättring för framtida studier.

CATD was not only adequate for analyzing the pursuit of erratically moving prey but also worked well when analyzing the pursuit of prey that remained stationary. It cannot be fully concluded that bats utilize the CATD strategy to successfully capture erratically flying prey. The angle remains relatively constant but does not exhibit a zero change in angle as by definition. The large forces experienced by the bat were seen when the bat began to rotate its body about its pitch axis or when the bat made a large turn. Moments were seen specifically when the bat began to bank into its last and final turn towards its target.; Digital recordings of three different species of bats were studied in this thesis to determine the forces and moments that were experienced throughout the bat's flight. The recordings were also studied to determine the pursuit strategies that were most effective for the bat to quickly capture its prey. A pursuit strategy is a strategic way to travel that will allow a pursuer to capture/approach their target the quickest. Therefore when a bat utilizes a particular pursuit strategy, it will adjust its position/ direction vector in a particular way that will allow it to approach its target very quickly. Data was collected directly from the video by manual collection utilizing Microsoft Visual Studio to extract frames, collect and record the data. This research was conducted to determine when throughout the flight the bat would experience significant forces and moments. The location and magnitude of the forces were reported along with an explanation of why the bat was experiencing a peak at each specific time. The forces and moments that the bat experienced thought-out the flight pursuit were calculated by relative velocity and acceleration calculations. In all four scenarios the bat experienced forces in relation to rotating its body about its center of mass. Forces were specifically seen when the bat periodically began to rotate its body before the final plunge to capture its prey. Prey avoidance and pursuit strategies were also studied and observed in this thesis which included the constant bearing and the constant absolute target direction. The intent was to determine which pursuit strategy bats use to quickly capture their prey. The constant bearing strategy is utilized to pursue prey moving along a smooth path, on the other hand the Constant Absolute Target Direction (CATD) pursuit strategy is utilized to capture erratically moving prey. For most of the bats analyzed, it was seen that the CATD strategy proved to be the preferred pursuit strategy.