Lipid oxidation has important consequences in the edible oil industry, producing compounds with sensory impact and thus reducing the economic value of the products. This work focused on the development of two Fourier transform infrared (FTIR) spectroscopy methods for the measurement of peroxide value (PV) and anisidine value (AV), representing the primary and secondary oxidation products of edible oils. / The infrared method developed for PV determination was based on a mathematical treatment by the partial least squares method of the information contained in the spectral region between 3750 and 3150 cm$ sp{-1}$. / The second method developed considered aldehyde content and anisidine value, a measure of secondary oxidation products. / The two methods developed are rapid ($ sim$2 min/sample) and have the advantage of being automatable. An infrared system coupled to a computer can collect the spectrum of an oil, analyze it and present a report without the need for personnel trained in FTIR spectroscopy. The cost of such a system would rapidly be absorbed through savings on personnel cost, time and chemical reagents required for conventional chemical methods and as such provides a useful advance in quality control methodology for the edible oils sector. (Abstract shortened by UMI.)

Graduation date: 2004

Thesis (M.Ed. (Education)) - Rhodes University, 2009. / Submitted in partial fulfillment of the requirements for the degree of Master of Education.

Thesis (Ph. D.)--University of Hong Kong, 1999. / Includes bibliographical references.

Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 137-149). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 137-149). Also available on the Internet.

Submitted in fulfillment for the Degree of Master of Technology (Biotechnology) in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, 2009. / Information regarding the nutritional value of wild food plants in Africa and current information varies from source to source. Prior to commercialization of wild foods the nutritional, ethnobotanical, medical, chemical, anthropological and toxicity requires investigation. Plants from the Amaranthaceae family were chosen because the family is characterized by several species which are used by indigenous communities as a source of nutrition in different plants of the world. The focus of this study was to investigate the nutritional and biological activities of three plants from the Amaranthaceae family viz. Achyranthes aspera, Alternanthera sessilis and Guilleminea densa that are considered famine plants. This study aimed to determine the nutritional value (proximate, minerals and vitamins), biological activity, toxicity and potential of a tissue culture system for three species from the family Amaranthaceae. Nutritional analysis comprised of determining moisture, ash, protein, fat, carbohydrate, dietary fibre and energy. Mineral analysis of calcium, copper, iron, magnesium, manganese, phosphorus, sodium and zinc was performed by microwave digestion and then analyzed by ICP Spectrophotometry. Vitamin A, Vitamin B1, Vitamin B2, Vitamin B3 and Vitamin C were also analyzed. For biological and safety analyses aqueous and methanolic extracts were prepared. Anti-oxidative and anti-inflammatory properties of the extracts were tested; antimicrobial activity was tested by evaluating the bactericidal, fungal effect and minimum inhibitory concentration on selected bacteria and fungi using the agar disk diffusion method. Anti mosquito potential was determined by setting up repellency, larvacidal assay and insecticidal assay. The safety and toxicity analysis was carried out by measuring cytotoxicity, toxicity and mutagenicity. The potential of an in vitro tissue culture system of A. aspera, A. sessilis and G. densa was determined using micropropagation. A. aspera indicated significant amounts moisture, ash, dietary fibre, protein, vitamin B1, vitamin B2, magnesium and manganese. Plant extracts of A. aspera had antibacterial activity against the Gram negative bacteria Esherichia coli, Pseudomas aeroginosa and Salmonella typhi; Gram positive bacteria Staphylococcus epidermis and Staphylococcus aureus. The methanolic extract had antifungal activity against Sacchromyces cerevisiae and exhibited significant free radical scavenging activity as well as 85% repellency against Anopheles arabiensis. The aqueous extract stimulated the growth of the K562 (Chronic Myclogenous Leukaemia) cell line and the plant extracts showed no mutagenicity or toxicity. A. sessilis indicated significant levels of ash, dietary fibre, protein, energy, vitamin A, vitamin B1, vitamin B2, vitamin B3, iron, magnesium and manganese present. Plant extracts of A. sessilis had antibacterial activity against Gram negative bacteria P. aeroginosa and Gram positive bacteria S. epidermis. The plant also showed antifungal activity against the yeasts S. cerevisiae and Candida albicans. The methanolic plant extract showed excellent antioxidant activity. The aqueous plant extract stimulated the growth of the K562 cell line and the plant extracts possessed no mutagenicity or toxicity. This plant grew well in a tissue culture system where it was propagated from callus to a fully grown plant able to survive in environmental conditions. G. densa has ash and dietary fibre, vitamin B2, vitamin B3 and iron. The plant extracts had antibacterial activity against Gram negative bacteria E. coli, P. aeroginosa and Klebsiella. oxytoca; Gram positive bacteria Baccilus stereathermophilus and S. aureus. The plant also has antifungal activity against C. albicans and significant repellency activity against A. arabiensis where it showed 100% repellency. This plant was not found to be mutagenic or toxic. The results obtained from this study show promising potential for the plants to be exploited as famine food plants. The nutritional value, biological activity and ability to micropropagate A. aspera, A. sessilis and G. densa indicates a good potential for purposes of harnessing biotechnological products.

Submitted by José Rodrigo de Araujo Guimarães (rodrigoagronomoal@yahoo.com.br) on 2018-08-06T14:27:57Z No. of bitstreams: 1 CARACTERIZAÇÃO FÍSICO-QUÍMICA E COMPOSIÇÃO MINERAL DE Pereskia aculeata Mill., Pereskia grandifolia Haw E Pereskia bleo (Kunth) DC..pdf: 1218066 bytes, checksum: 25200ec41273cd0bdc54b9ad8dd8601d (MD5) / Approved for entry into archive by Ana Lucia de Grava Kempinas (algkempinas@fca.unesp.br) on 2018-08-06T14:40:21Z (GMT) No. of bitstreams: 1 guimaraes_jra_dr_botfca.pdf: 1218066 bytes, checksum: 25200ec41273cd0bdc54b9ad8dd8601d (MD5) / Made available in DSpace on 2018-08-06T14:40:21Z (GMT). No. of bitstreams: 1 guimaraes_jra_dr_botfca.pdf: 1218066 bytes, checksum: 25200ec41273cd0bdc54b9ad8dd8601d (MD5) Previous issue date: 2018-06-08 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Ora-pro-nobis é uma planta alimentícia não convencional de elevado potencial nutricional utilizada, principalmente, em comunidades tradicionais como alimento proteico. Objetivou-se neste trabalho realizar a caracterização físico-química e análise de minerais em Pereskia aculeata Mill., Pereskia grandifolia Haw. e Pereskia bleo (Kunth) DC. plantas conhecidas como ora-pro-nobis. O experimento foi conduzido na Fazenda Experimental Lageado, e as avaliações realizadas no Departamento de Horticultura da Faculdade de Ciências Agronômicas da UNESP. Para este estudo foram utilizadas mudas das três espécies de ora-pro-nobis e conduzidas em campo com espaçamento 1,0 x 1,0 m entre fileiras e plantas. O experimento foi constituído de um total de 168 plantas, sendo 56 de cada espécie, P. aculeata, P. grandifolia e P. bleo, respectivamente. Foram realizadas análises físico-químicas (sólido solúveis, pH, acidez titulável, açúcares redutores, teor de umidade e cinzas, fibra bruta, gordura, ácido ascórbico, compostos fenólicos, pigmentos - clorofila a, clorofila b, antocioninas e carotenoides), proteína e minerais (P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn). Pode-se concluir que as três espécies avaliadas apresentaram-se como excelentes fontes de proteína, fibras, ácido ascórbico, ferro, zinco, cálcio e coloração atraente, por suas folhas possuírem cores verde escura. P. bleo apresentou maior teor de ácido ascórbico, teor de umidade, alto teor de lipídeos, ferro e zinco. P. grandifolia apresentou maiores teores de proteína e compostos fenólicos e P. aculeata apresentou maiores teores de fibras. Por fim, esta caracterização pode subsidiar estudos futuro para inclusão em dietas alimentares, por estas plantas apresentarem elevado potencial nutricional. / Ora-pro-nobis is an edible plants with a high nutritional potential, mainly used in traditional communities as a protein food. The objective of this work was to perform the physicochemical characterization and analysis of minerals in Pereskia aculeata Mill., Pereskia grandifolia Haw. and Pereskia bleo (Kunth) DC. plants known as ora-pronobis. The experiment was conducted at the Experimental Farm Lageado, and the evaluations carried out at the Department of Horticulture of the Faculty of Agronomic Sciences of UNESP. For this study, seedlings of the three ora-pro-nobis species were used and conducted in the field with spacing 1.0 x 1.0 m between rows and plants. The experiment consisted of a total of 168 plants, 56 of each species, P. aculeata, P. grandifolia and P. bleo, respectively. Physical and chemical analyzes (solid soluble, pH, titratable acidity, reducing sugars, moisture content and ashes, crude fiber, fat, ascorbic acid, phenolic compounds, pigments - chlorophyll a, chlorophyll b, anthocyanins and carotenoids), protein and minerals (P, K, Ca, Mg, S, B, Cu, Fe, Mn, Zn). It can be concluded that the three evaluated species presented as excellent sources of protein, fibers, ascorbic acid, iron, zinc, calcium and attractive coloring, because their leaves have dark green colors. P. bleo presented higher content of ascorbic acid, moisture content, high lipid content, iron and zinc. P. grandifolia presented higher levels of protein and phenolic compounds and P. aculeata had higher fiber contents. Finally, this characterization may support future studies for inclusion in dietary diets, because these plants present high nutritional potential.

Mycelial pellet formation by filamentous fungi is one of the most researched topics in fungal biotechnology research. Pellets are generally formed as a result of a complex interaction process through the influence of many cultivation factors such as inoculum size, pH, dissolved oxygen level, agitation system, nucleating agents, additives, trace metals, CO2, temperature, reactor types, carbon substrate, rheology, culture modes, fermenter geometry, nitrogen and phosphate levels etc. Each factor has varying effects on the growth morphologies of different fungal species. Fungal growths in the form of pellets have several advantages and pose a potential solution to overcome the problems associated with the filamentous fungal growth in large scale industrial bioreactors. The aim of the present work was to study pellet formation of edible filamentous fungus Neurospora intermedia, focusing on the molecular aspects of the fungal pellets with special interest to investigate the role of cell signaling second messenger cyclic 3', 5’-adenosine mono- phosphate (cAMP). It was found that Neurospora intermedia stimulate cAMP in the pellet form than filamentous form. The industrial applications of fungal pellets for generating value added products were also studied and observed fermentation in individual and co fermented first and second-generation ethanol substrate, showed an ethanol yield maximum of 0.25 ± 0.05 g/g dry substrate. The growth of fungal pellets in presence of inhibitors (such as acetic acid, HMF and furfural) resulted in about 11% to 45% increase in ethanol production as compared to filamentous forms, at similar growth conditions in the liquid straw hydrolysate.

Orientador: Renata Tieko Nassu / Banca: Rubens Bernardes Filho / Banca: Daniela Cardoso Umbelino Cavallini / Resumo: No mercado brasileiro, a cadeia da ovinocultura vem ganhando destaque como uma atividade em expansão dentro do agronegócio. Durante a vida de prateleira da carne fresca podem ocorrer mudanças físico-químicas, microbiológicas e sensoriais indesejadas. Devido a este fato, para atender necessidades do consumidor, tais como praticidade, maior tempo de conservação, entre outros, é necessário prolongar a vida útil da carne, permitindo responder ao intervalo de tempo entre a produção e a distribuição dos produtos. Uma alternativa é o uso de filmes de revestimentos comestíveis, que podem ser aplicados como embalagens primárias. Objetivo: O objetivo deste trabalho foi avaliar o efeito de revestimentos à base de quitosana e zeína em carne ovina in natura embaladas à vácuo e armazenadas por um período de 57 dias em duas diferentes temperaturas. Material e métodos: O estudo foi dividido em dois experimentos. No experimento 1, foram testados sete revestimentos à base de zeínas com diferentes plastificantes em carne ovina embalada à vácuo. Foi estudado o efeito desses revestimentos na qualidade da carne ovina em suas características físico-químicas (pH, cor instrumental, capacidade de retenção de água, perda de peso por cocção, força de cisalhamento e oxidação lipídica) e também foi feito o teste de halo de inibição das soluções filmogênicas com o intuito de selecionar a melhor formulação para o segundo estudo. No experimento 2 foram avaliadas as características físico-químicas, microbioló... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Objective: The objective of this study was to evaluate the effect of daily orange juice consumption on cardiometabolic risk parameters in subjects with metabolic syndrome. Methods: The study design was parallel, controlled and randomized, and all participants were previously classified with metabolic syndrome (ATPIII, AHA / NHLA). The volunteers were oriented to the consumption of a nutritionally balanced diet, energetically compatible with current weight. The participants were randomly divided into: Control Group (n = 36), who underwent nutritional orientation throughout the experiment, and Orange Juice Group (n = 36), with nutritional orientation followed by ingestion of 500 mL / day of 100% whole orange juice for 12 weeks. Biochemical, metabolic, anthropometric, hemodynamic, dietary parameters were analyzed at regular intervals throughout the 12 weeks of intervention. Results: Individuals in both groups presented a reduction in waist circumference (6%), visceral fat area (4.5%), systolic and diastolic blood pressure (8% and 9%) and glycemia (3%) (p < 0.05), without changes in muscle mass and insulin levels. Both groups presented similar results for energy consumption and macronutrients, and vitamin C and folic acid intake were 133% and 43%, respectively, higher in the orange juice group than in the control group at the end of the 12 weeks of intervention. Conclusion: It was concluded, therefore, that the inclusion of orange juice in the diet of patients with metabolic syndrome did not promote adverse effects on body composition, cardiometabolic or hemodynamic risk factors, with a reduction on number of individuals diagnosed with metabolic syndrome, in addition to improving the quality of diet... (Complete abstract click electronic access below) / Mestre