The goal of this research was to develop gypsy moth egg mass sampling plans that reflect the influence of habitat, changes in egg mass distribution, and provide populations densities or density categories for making control decisions. Sequential egg mass sampling plans for gypsy moth management programs in urban and suburban habitats were developed from 0.01 ha fixed-radius plot samples collected in Loudoun, Fairfax, and Arlington Counties, Virginia. The sampling plans were develop from Wald's sequential probability ratio test and is based on a negative binomial distribution. Operating characteristic and average sample number curves were used to determine the acceptability of the sampling plans. Three sampling plans were developed for the action thresholds of 618, 1,236, and 2,471 egg masses/ha. The use of binomial sampling for low density (<618 egg masses/ha) gypsy moth populations in continuously forested habitats was examined. Fixed- and variable-radius plot egg mass samples were collected in 28 study areas in Virginia, Maryland, and Massachusetts. The relationship between egg mass density and the proportion of trees with zero egg masses was developed. Binomial sampling resulted in a higher relative variability and lower relative efficiency than the fixed- and variable-radius plot sampling method. Binomial sampling was determined not to be an effective sampling method for gypsy moth populations below 618 egg masses/ha. Fixed- and variable-radius plot egg mass samples were taken when leaves were present (summer) and absent (winter) in 136 sample sites in Virginia. A significant difference between summer and winter counts was determined. The relationship between summer and winter counts was quantified using nonparametric Statistics. Winter egg mass counts were found to be 14 to 34 percent higher than summer egg mass counts . The probability of a summer egg mass count exceeding an action threshold was constructed by fitting a logistic curve to empirical data for the action thresholds of 618 and 1,236 egg masses/ha. Egg mass counts need to compensate for differences between summer and winter counts. Alternatively, the probability of a summer egg mass count exceeding an action threshold should be utilized. / Master of Science

The white perch (Morone americana) is a highly successful invader of freshwater systems, with negative consequences to some resident fishes. White perch are ovivores and may prey upon as well as compete with juvenile sportfishes. Since 1988, an introduced population of white perch has proliferated in the 19,790-ha Kerr Reservoir, NC-VA, potentially threatening popular sport fisheries for largemouth bass (Micropterus salmoides), crappie (Pomoxis spp.), and self-sustaining striped bass (Morone saxatilis). Trophic relationships between white perch and resident sportfish were examined in the Kerr Reservoir system to determine the white perch's impact on them. Striped bass eggs were found in the stomach contents of white perch collected from the Roanoke River during their concurrent spawning runs in May, and at times egg predation was intense (frequency of egg occurrence in white perch individuals up to 100 %). However, modeling simulations indicated that observed densities of white perch in the Roanoke River during the peak spawning period of striped bass (May) were too low to have a substantial effect on striped bass recruitment. Crappie eggs were found in the diets of white perch collected from Kerr Reservoir during April, but the significance of this predation was not determined. Trophic overlap (Schoener's Index) was high (> 0.5) between age-0 white perch and age-0 crappie, largemouth bass, and striped bass in June, but only remained high between white perch and crappie in the remainder of the growing season (July – September). After June, largemouth and striped bass were primarily piscivorous, whereas white perch and crappie remained primarily invertivorous. Trophic overlap was high between adult white perch and adult crappie (> 0.6), but not between white perch and any other species of adult sportfish. The utilization of white perch by adult piscivores (Ictalurids, Pomoxis spp., Moronids, and Percids) as a food source was low (< 2% of diets by weight). Piscivorous sportfish primarily ate clupeids, which are highly abundant in Kerr Reservoir. Analysis of sportfish performance before (1974 – 1987) versus after (1996 – 2005) white perch establishment indicated no change in abundance and growth of striped bass and largemouth bass. Performance data for crappie prior to white perch introductions were unavailable, but observed egg predation by and trophic overlap with white perch suggests that crappie have the highest potential to suffer deleterious interactions with the white perch. Abundance of white bass has significantly declined in years since white perch introductions. Future research in Kerr Reservoir should concentrate on interactions between white perch and these two species. / Master of Science

For 36 generations, White Leghorn chickens were selected for high (HAS) or low (LAS) antibody response to sheep red blood cells. The focus of this thesis was to investigate correlated responses in reproductive soundness and egg quality resulting from that selection. Forty-five hens and 25 roosters from each antibody line were used. In hens, commencement and intensity of lay, and egg quality, were analyzed; in both sexes, length of fertility was considered. Hens and roosters were mated to an intercross line to avoid confounding selection with sex effects. The LAS line was more reproductively sound, commencing lay at a younger age (11.67 ± 3.53 d; P < 0.001), lighter body weight (-169.46 ± 40.20 g; P < 0.001) and with greater intensity (2.68 ± 0.25%; P = 0.001) than the HAS line. Additionally, the LAS line had a greater length of fertility (hens: 2.43 ± 0.55 d; P < 0.001; roosters: 3.11 ± 0.71 d; P < 0.001). In contrast to their poorer reproductive soundness, the HAS line had superior egg quality compared to the LAS line. Egg shape index (4.12 ± 0.55; P < 0.001) and albumen height, measured in both mm (0.27 ± 0.12 mm; P < 0.001) and Haugh units (1.89 ± 0.91; P = 0.04), were superior in HAS hens. Selection for increased antibody response appeared to compromise reproductive soundness, perhaps due to limitations in available resources. However, the selection did not compromise egg quality. / Master of Science

Two experiments were conducted to examine the effects of type of milk (whole; nonfat; nonfat, 70% lactose-reduced) and type of egg (fresh, whole egg; egg substitute) on the quality of baked custards. The egg substitute was a combination of dried egg whit e solids, dried low-fat, low-cholesterol egg yolk solids, and xanthan gum. Custard formulations served as prototypes for use in studies conducted in the Department of Food Science and Technology, Virginia Tech. In those studies, the effects of processing parameters on the quality of the custards were examined. The goal of all the studies was to develop shelf-stable lactose-reduced custard mixes that have potential for use in the foodservice industry. In Experiment I, chemical, physical, and sensory characteristics of a dessert type baked custard, made with sucrose, were examined. In Experiment II, the same characteristics were examined for a cheese flavored, entree type custard made without sucrose. Results indicate that use of nonfat and nonfat, 70% lactose-reduced milk in place of whole milk decreased significantly total fat and cholesterol concentrations in both types of custard mixes. Lactose-reduced milk also decreased lactose and increased galactose concentrations. The egg substitute decreased total fat and cholesterol concentrations and increased protein concentration. In general, lactose reduction had little effect on the physical and sensory characteristics of both types of custards, but the egg substitute affected these characteristics. Custards made with the egg substitute had less intense yellow color and greater gel strength than those made with fresh, whole egg. These custards also were less bright and more uneven in color and had weaker egg flavor and aftertaste. Based on results of this study, development of a lactose-reduced custard is feasible. However, additional studies are needed to examine potential for use of the formulations in the foodservice industry. Because the mixes could be used as bases for other types of products, such as custard pies, filled pastries, and quiches, studies that focus on development of those products could help define the niche for the mixes in the foodservice industry. Because of the trend in today1s market for reduced-fat, reduced-cholesterol foods, additional studies could be done to examine the effects of various types of egg substitutes on the quality of the custards. / Master of Science

A precision biotic (PB) in standard and reduced CP diets, and a protease (PRO) used alone and in combination with PB in the reduced CP diet were evaluated. Effects on performance, digestibility, and blood biomarkers were determined in Dekalb White hens from 24 to 42 weeks of age. Nine diets were fed: 1) a standard diet with a 17.5% formulated CP level (T1); 2) T1 with 500ppm PB (T2); 3) T1 with 700ppm PB (T3); 4) a reduced CP diet with a 15.5% formulated CP level (T4); 5) T4 with 500ppm PB (T5); 6) T4 with 700ppm PB (T6); 7) T4 with 33g/MT PRO (T7); 8) T4 with 33g/MT PRO and 500ppm PB (T8); and 9) T4 with 33g/MT PRO and 700ppm PB (T9). There were no overall differences for feed conversion, but the response of T9 over time suggests longer periods may result in improved feed conversion.

Commercial laying hens are heavily selected for increased egg production, but little selection has occurred for resistance to extreme temperatures. The objective of the experiment was to determine the effects of a daily cyclic heat stress (HS) on performance, body temperature, egg quality, nitrogen corrected apparent metabolizable energy, and blood chemistry in laying hens at acute and chronic time points. In total, 407 pure line laying hen pullets (18 wk) were sourced from Hy-Line International and housed individually for the duration of the experiment. Feed intake, egg production, and body weights were significantly decreased while egg weight and feed efficiency increased due to HS exposure. All egg quality parameters except yolk weight were decreased by HS exposure, including Haugh unit, eggshell and albumen weights. Egg yolk weights were increased over the first 2 wk of HS before falling. Blood chemistry was affected by HS resulting in respiratory alkalosis likely caused by increased respiration and evaporative cooling. There was a decrease in the PCO2, an increase in pH and a reduction in iCa in the blood within 4 to 6 hours of HS when compared to pre-HS levels. The data indicate that the hens were negatively affected by HS and the response was different for acute and chronic time points. At the acute time point the changes to the hen's physiology were caused by altered blood chemistry, but after chronic exposure, the low feed intake seemed to influence the responses. / Master of Science / High environmental temperatures can cause laying hens to become heat stressed, including reduced egg production, egg quality, and body weight, and in some cases increased mortality. Two mechanisms to reduce heat production and increase heat dissipation are reduced feed intake and increased respiration rate during heat stress. The reduced feed intake can result in insufficient nutrients to maintain egg production and increased respiration rate can change the blood chemistry, reducing ionic calcium and ultimately causing thinner eggshells. The most efficient way to reduce the negative effects of heat stress is by avoidance through controlling the environmental temperature, however, above certain temperatures cooling methods fail. If heat stress conditions are reached, it is important to understand the genetic ability of the hen to minimize the negative consequences of elevated temperature. Therefore, the effects of a four-week heat stress were determined with a focus on hen performance, egg quality, and blood chemistry parameters in laying hens. Some parameters such as blood pH and ionized calcium, shell weight, and body temperature were negatively affected within four to six hours while others, such as shell thickness, Haugh unit, and egg production took longer to be affected. By the end of the experiment blood pH returned to pre-heat stress levels but feed intake was significantly reduced over the 4 wk HS period. This could indicate that the laying hens began to acclimate to the high environmental temperatures but were not able to completely overcome the negative effects.

Doctor of Philosophy / Department of Entomology / J.P. Michaud / James R. Nechols / The manner in which organisms allocate reproductive resources for reproduction is a central question with respect to life history theory. The main objectives of this research were to i) examine lifetime patterns of reproductive allocation in the lady beetles Coleomegilla maculata (DeGeer) and Hippodamia convergens (Guérin-Menéville) (Coleoptera: Coccinellidae) while manipulating environmental conditions that affect female body size (i.e., larval food supply), ii) to study the interaction between factors underlying female body size and the resources available during reproduction, and iii) to explore the maternal effects of female size and age on the development and survival of progeny. When different size classes of females were produced and adult females were maintained with unlimited food, there were no differences in egg size across female size in C. maculata, but egg size increased over time in all females. In H. convergens, only larger females increased egg size over time, and they laid larger eggs, on average, than did small females. Maternal body size was positively correlated with the number of eggs laid per day in both species. When three size classes of females were subjected to a fluctuating food supply as adults, female size was again positively correlated with egg and daily fecundity. Whereas both species varied daily fecundity in response to adult food supply, egg size was unaffected and demonstrated a fixed pattern of change with female age and species-specific effects of maternal body size. To observe maternal effects in H. convergens, three female size classes were again produced and progeny were reared from three different periods of each female‟s reproductive life. Offspring from later oviposition days and larger females developed faster and achieved larger adult size than those reared from earlier oviposition days. Egg size showed inconsistent correlations with developmental parameters and adult progeny size, so other, more cryptic, maternal signals were inferred to signal phenotype development in progeny. A fixed program of producing faster-developing offspring that mature to larger sizes late in the oviposition cycle is adaptive for exploiting ephemeral aphid blooms that exhibit predictable dynamics of declining prey abundance and increasing competition. In the case of H. convergens, resource limitation during development constrained not only body size, fecundity and egg size, but also maternal ability to manipulate progeny phenotypes.

O ovo tem sido estudado por sua riqueza nutricional, por apresentar interesse industrial, como matéria-prima, e pelo seu elevado conteúdo de colesterol. Ao mesmo tempo, por sua susceptibilidade à contaminação por salmonela, principalmente, é proposta a irradiação ionizante como medida sanitária. O colesterol está sujeito à oxidação, facilitada por vários fatores, entre os quais a radiação ionizante. Os óxidos de colesterol formados, por sua vez, apresentam propriedades biológicas prejudiciais à saúde, relacionadas com a aterogenicidade, citotoxicidade, carcinogenicidade e mutagenicidade, além de outras manifestações. Os objetivos deste trabalho foram avaliar o efeito da radiação ionizante sobre o pH, viscosidade e cor, além da estabilidade oxidativa do colesterol, em ovos crus armazenados e processados. Com o aumento nas doses utilizadas (1, 2 e 3 kGy) houve redução na viscosidade da clara e na cor da gema, além do aumento da oxidação lipídica, medida através das substâncias que reagem ao ácido tiobarbitúrico (TBARS). Parâmetros como umidade, lípides totais e colesterol das gemas não foram influenciados. No caso da umidade e do colesterol, houve alteração significativa pelo armazenamento (30 dias, a 4ºC). O somatório dos óxidos analisados não variou com a irradiação, só individualmente, contudo variaram com o armazenamento. O processamento térmico provocou um aumento significativo das TBARS, mas apesar disso, o somatório dos óxidos não diferiu entre os tratamentos. / The egg have being studied due its nutritional wealth, for show industrial interest as a raw material, e due its higher cholesterol content. At the same time, due its susceptibility to contamination mainly with salmonella, it is being proposed the ionizating radiation as a hygienic measure. Cholesterol is subject to oxidation, that it is facilitated by several factors, among them ionizating radiation. Formed cholesterol oxides, by its turn, show harmful biological properties to human health, as atherogenicity, cytotoxicity, carcinogenicity and mutagenicity, among others. The objectives of this work were evaluate the effect of ionizating radiation over pH, viscosity and color, besides the oxidative stability of cholesterol, in storaged and processed crude eggs. With the increasement of used doses (1, 2 and 3 KGy), there was an reduction in the viscosity of the egg white and in the color yolk egg, besides the increase in lipidic oxidation, measured through tiobarbituric acid-reactive substances (TBARS). Specifications as humidity, total lipids and egg yolk cholesterol were not influenced. In the subject of humidity and of cholesterol, there was an meaningful alteration due storage (30 days in 4ºC). The sum of the analyzed oxides didn\'t variate with the irradiation, only individually, although it did vary with storage. The themical processing caused an meaningful increase of TBARS, but despite this, the oxides sum didn\'t differed between treatments.

I concern on african mouthbrooding fishes (Cichlidae) and the possible role of egg-dummies during their courtship (Wickler's theory). I am bringing list of african cichlid fishes with information about kind of parental care, courtship ritual and presence of egg-dummies and I am giving this ethoecological information to phylogenetic context.

In the nature, crayfish are represented like predators or a part of food base of some fish. Also in free waters they acts as detrivors and are an important element in the nutrient and energy circle in aquatic ecosystem. The crayfish weren't able to avoid negative impacts of anthropogenic activities such as water pollution and devastation of habitats. For these reasons reproduction of the crayfish is necessary in hatcheries and farms. The main aim of my work was to study the reproductive biology of the crayfish focused on the process of mating, the interval between mating and laying eggs, fertilization and morphological structure of the male and female gametes. Among the secondary objectives there was included using of repeated electrostimulation of the crayfish and the possibility of hybrids of the noble crayfish (Astacus astacus) and the narrow-clawed crayfish (Astacus leptodactylus). The observation of the period of reproduction was realized on representativies of the noble crayfish and the narrow-clawed crayfish throughout natural mating in the tanks. The structure of gametes was observed on the samples taken from thenoble crayfish (Astacus astacus), the narrow-clawed crayfish (Astacus leptodactylus), the signal crayfish (Pacifastacus leniusculus), the red swamp crayfish (Procambarus clarkii) and spinycheek crayfish (Orconectes limosus). The experiments were carried out during the autumn season in 2012 and 2013. The results of observations of the reproduction of crayfish made us clear as for the period and intervals for all phases of the process of mating crayfish. For females the noble crayfish and the narrow-clawed crayfish was found oviposition without the presence of males. Their individual components have been described by using ultrastructure of males and female gametes. Repeated electrostimulation showed at the family Astacidae as a suitable tool for collecting large mass of sperm material. Hybridization experiment proved that it can occur to mate the two species of crayfish in areas where they occur simultaneously. These experiments helped to further knowledge of reproductive biology of crayfish.