Eldana saccharina Walker (Lepidoptera: Pyralidae) is an indigenous insect widely distributed throughout Sub-Saharan Africa that is a major pest of sugarcane in southern Africa. Studies have shown that populations from West Africa have distinct behavioural differences compared to populations from eastern and southern Africa. In addition, the parasitoid guilds attacking populations in these regions are markedly different. This marked behavioural and parasitoid guild variation evoked a hypothesis of genetic diversification. To evaluate this hypothesis a project on the phylogeography of E. saccharina was initiated. The project was planned to include sampling of as many regions as possible in its known range in Africa, to obtain specimens of E. saccharina for genetic analysis. When these surveys were initiated in Ethiopia, it was found that there was no published literature available on the occurrence of stem borers in Ethiopian sugarcane. It was thus clear that no stalk borer/parasitoid surveys had been completed in either sugarcane or any large grass and sedge indigenous hosts in Ethiopia. The study was thus expanded beyond the investigation of only the genetic diversity of E. saccharina, to include area-wide surveys to determine ecological aspects of the borer complex in suspected host plants, including sugarcane, in Ethiopia. In this way the host plant range and distribution of E. saccharina and other sugarcane borers in Ethiopia in particular could be determined, samples for a larger phylogeography project could be collected, and the insect's impact on sugarcane could be assessed. Quantified area-wide surveys of the sugarcane estates and small-scale farmer fields of Ethiopia were conducted between December 2003 and February 2004. The surveys verified the presence of four lepidopteran stem borer species on Ethiopian sugarcane. These were Chilo partellus Swinhoe (Lepidoptera: Crambidae), Sesamia calamistis Hampson (Lepidoptera: Noctuidae), Busseola fusca Fuller (Lepidoptera: Noctuidae) and Busseola phaia Bowden (Lepidoptera: Noctuidae). The surveys indicated that Busseola species are the major and most widely distributed sugarcane stem borers in sugarcane farms of Ethiopia. Over 70% of the peasant sugarcane fields visited were infested by these borers, with the highest levels of infestation (35% and 50%) being in the northern and western part of the country, respectively. Busseola was also the predominant stem borer of sugarcane in two of the three estates (Wonji and Finchawa). Chilo partellus and S.calamistis were recovered in very low numbers at all the commercial estates and from peasant farms in the western part of Ethiopia. However, C. partellus was the predominant sugarcane stem borer in lowland areas of northern, southern and eastern parts of the country. Eldana saccharina was recovered from large sedges in waterways of Metehara and Wonji sugar estates in the central part of the country, and sedges growing around lakes in northern and southern Ethiopia, but not from sugarcane anywhere in Ethiopia. The phylogeographic study conducted on E. saccharina populations from eleven countries of Africa clearly showed the population structure of the insect within the continent. Five hundred and two base pairs of the mitochondrial DNA (mtDNA), corresponding to the Cytochrome Oxidase subunit I (COl) region, were sequenced to clarify phylogenetic relationships between geographically distant populations from eastern, northern, southern and western Africa. Results revealed that E. saccharina is separated into four major populations corresponding to their geographical location, i.e. West African, Rift Valley and two southern African populations. Sequence divergence between the four populations ranged from 1% to 4.98%. The molecular data are congruent with an isolation by distance pattern except for some of the specimens from eastern and southern Africa where geographically close populations are genetically distant from each other. Geographical features such as the Rift Valley and large water bodies in the continent seem to have a considerable impact on the genetic diversity in E. saccharina. Identification of field-collected stem borer specimens was done using classical taxonomic techniques, except for Busseola spp. where DNA barcoding was used. As field-collected larval material of Busseola died before reaching the adult stage, identification of species using adult morphology was not possible. 'Sequence divergence in the COl gene was used as a tool to identify the species of Busseola attacking Ethiopian sugarcane. Partial COl sequences from Ethiopian specimens were compared with sequences of already identified noctuid species from the East African region. Results of the sequence analysis indicated that the Busseola species complex in Ethiopian sugarcane comprised B. fusca and B. phaia. Sequence divergences between Ethiopian Busseola species was as high as 5.0 %, whereas divergences within species were less than 1% in both species identified. Several larval parasitoids, bacterial and fungal diseases of stem boring caterpillars were also recorded in Ethiopian sugarcane. Amongst these was Cotesia flavipes Cameron (Hymenoptera: Braconidae). This exotic parasitoid has been introduced into several African countries for the control of C. partellus in maize and sorghum, but had never been released in Ethiopia. To investigate the origin of C. flavipes in Ethiopian sugarcane, molecular analyses were conducted on Ethiopian specimens from sugarcane and specimens of C. flavipes from different countries of Africa released from the Kenyan laboratory colony, again using COl sequences. Results of the analysis revealed that the C. flavipes population that had established in sugarcane fields of Ethiopia was similar to the south east Asian populations released against C. partellus in maize in other parts of Africa, and different from other populations of this species, providing evidence that the Ethiopian C. flavipes is likely to be a descendant of the original Pakistani population that was released in different parts of Africa. The study reveals the importance of lepidopteran stem borers in sugarcane production in Ethiopia and highlights the role of molecular methods in species identification and determining phylogenetic relationships. More importantly, this study establishes the continental phylogeographical pattern of the indigenous moth, E. saccharina. The impact of geological events, geographic barriers and cropping systems on the evolution, distribution and abundance of stem borers are discussed. Future areas of research for understanding more about the phylogeographic relationships of E. saccharina and management of stem borers are discussed. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Since becoming a pest in graminaceous crops in Africa, the African sugarcane stalkborer Eldana saccharina Walker has been the subject of much study. Its very cryptic habits have precluded more commonly available control measures being used against it. Biological control is regarded as a viable control option, but the apparent paucity of parasitoids in graminaceous crops leads to E. saccharina being regarded as lacking parasitoids, and thus not a good candidate for biological control in the classical sense. In contrast, this project argues that interactions in indigenous hosts of E. saccharina had been ignored, and that classical biocontrol principles and basic ecological theory could be applied by the discovery, collection and introduction of parasitoids from its indigenous habitat to its newly adapted habitat, sugarcane. The habitat offered by Cyperus papyrus L. was shown to be heterogenous both temporarily and spatially. Umbels, from young through mature to senescent, were available in the same proportion for colonisation throughout the year. Umbels with sexual reproductive stages (seeds) were present from early spring into late summer, and provided an additional component to the already heterogenous environment. Young umbels, in addition, developed from rhizomes in an environment regarded as sub-optimal for photosynthesis, until they reached the canopy. All stages of umbels were attacked by E. saccharina, but larvae were only found in rays of umbels and in the apex of the culm, which was the meristematic area for rays, both high nutrient areas. Young umbels were never found with borer pupae, only smaller larvae, indicating that E. saccharina development matched growth of young umbels until they reached canopy height. Also, the majority of borings found were occupied, indicating that infestation of young umbels was recent. All stages of E. saccharina development were found in mature umbels, which were also most abundant at anyone time. Numerous empty borings were found in addition to those occupied, indicative of past occupation by E. saccharina. Very few young larvae were found in old umbels, the majority of life stages found being pupae or empty pupal cases, and also many empty borings, showing that old umbels were not suitable for E. saccharina development. A guild of parasitoids which comprised Orgilus bifasciatus Turner, the most common parasitoid of small and smaller medium E. saccharina larvae, Goniozus indicus Ashmead the most common parasitoid of larger medium and large larvae, and an entomogenous fungus Seauveria bassiana (Sals.) Vuill. attacking all life stages of E. saccharina was found. Three uncommon parasitoids of smaller saccharina were also found, viz. Sassus sublevis (Granger), Iphiaulax sp. and Venturia sp. The former three natural enemies were instrumental in depressing a major outbreak within two months of it being observed and then maintaining the host population at a lower level in C. papyrus. G. indicus and B. bassiana were most effective during the summer and autumn months, and O. bifasciatus most effective during the winter months. This study supports the hypotheses that the apparent paucity of parasitoids and lack of biological control success thus far against E. saccharina in sugarcane has been because very little was known about its ecology and biology in its numerous indigenous host plants, and that studies of the latter factors coupled with ecological theory could enhance biological control programmes against this borer. As more indigenous host plants are investigated in the same way as has been done with C. papyrus, more will become known of natural enemies of E. saccharina. Parasitoid guilds could be selected, even from rare parasitoids in the more stable indigenous habitats, which would provide control in the unstable habitat of sugarcane. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1994.

The complexity of the behaviour of neonate Eldana saccharina Walker (Lepidoptera: Pyralidae) larvae and the limited information on their response to the morphological characteristics of South African sugarcane varieties was the primary justification to study antixenotic/antibiotic effects on larval behaviour. Laboratory experiments were conducted with stalk segments in plastic jars inoculated with larvae and in a metal cage covered with gauze. In jars, the larvae were observed until they penetrated the stalks. After 14 days, the stalks were dissected and larvae weighed. In all varieties, larvae moved directly to the node after inoculation and penetrated the stalk through leaf scars and buds. No significant differences in larval mass were observed among varieties. In cage experiments different parts of the node, namely the rind below the wax band; the bud; and the root primordia were tested. There was a clear indication that rind hardness and the budscale properties are associated with varietal resistance and only affect early instars. The experiments were repeated using whole cane plants in a glasshouse. The results were similar to those of laboratory experiments. In the Insect Rearing Unit, scraped waxes from different varieties were incorporated into the diet. Larval masses from different diets showed significant differences among varieties, but they did not conform to the known resistance ratings, as cane varieties N12 and N21 showed high susceptibility, instead of resistance. Dispersal behaviour of neonates shortly after hatching was investigated in 'mobility experiments' conducted on live cane plants. Mobility is important because the more time neonates spend wandering around on the stalk surface or on exposed parts of the plant, the more vulnerable they are to predation and other adverse factors that may reduce their survival. Experiments to test stalk penetration by larvae on the node showed that neonates required a softer food source before attacking the hard nodal parts. Second and third instar larvae were used subsequently to the mortality of all neonates fed on the rind, which in turn resulted in non-significant differences, suggesting that feeding on debris and/or leaves is critical to the survival and penetration of larvae into the sugarcane stalk. Incorporation of the characteristics tested in these experiments aims to reduce the number of larvae that penetrate the stalk and to expose them for longer on the surface where their numbers may be controlled by predators and insecticides. The resistant varieties used in these experiments have high fibre and less sugar, but newer varieties, such as N29 and N33 incorporate both high resistance and high sucrose yield, which are the two key elements for optimised sugar production. Chemical characteristics of the plants need to be taken into consideration as high sucrose is seldom found in fibrous varieties. Leaf sheath tightness is another characteristic that would go well with leaf sheath hairiness, because though not tested in this work-would make it difficult for the larvae to get to the smooth adaxial surface of the leaf. The hardness of trichomes is another feature that needs to be investigated, because a variety may have dense, but soft pubescence that does not repel even the most sensitive larvae, neonates. At present, integrating plant resistance with cultural control, i.e. field hygiene etc. is cost-beneficial for the sugar industry. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2002.

The eldana stalk borer (Eldana saccharina Walker) is the most serious pest of the Southern African sugarcane industry, and it is imperative that effective control measures are available to minimize economic damage. Because conventional control methods have had limited success, cultivar resistance is seen as the most viable method of controlling infestation. However, due to the space- and time-consuming nature of the present screening methods, only small numbers of cultivars can be tested relatively late in the Plant Breeding selection programme. Increased resistance in breeding and selection populations is therefore slow. Buds are a preferred entry point of eldana larvae as they are softer than the rind that is present on the rest of the stalk surface. Preliminary results by other workers suggested that near infrared spectroscopy (NIRS) could provide a rapid screening method for the chemical profile in bud scales, the outer coating of buds and therefore the first contact point of an invading larva. If feasible, analysis of samples using this method could be done in the South African Sugar Experiment Station's (SASEX) stage two selection trials, providing an early indication of eldana resistance on large numbers of cultivars, without the necessity of separate trials. However, knowledge of how environments, position of bud scales on the stalk and age affect NIRS is required in order to determine the feasibility of the method. Planting of a trial with an identical set of genotypes across a range of environments, sampled at a number of ages, would provide the necessary information on environmental effects, whilst simultaneously providing the necessary range of samples to develop a calibration between bud scale chemical profiles and eldana resistance ratings. Inheritance patterns of the characteristics being measured is also required if they are to be used in a breeding programme. The original work by Rutherford (1993) was carried out on only five calibration sets (a set of standard clones with relatively well-known eldana resistance ratings), and different sets were not comparable due to what was assumed to be environmental differences between calibration sets. One aspect of the current experiment was to examine more closely the effect of genotype x environment interaction (G x E) on the performance of the NIRS technique under a range of conditions. Two sites were chosen to represent the conditions encountered in trials carried out by SASEX. The crops were sampled at three ages, representing the range of ages at which sugarcane is harvested in South Africa. Two locations on the stalk were also examined, top and bottom, for removal of bud scales, based on the assumption that aging of bud scales may affect chemical composition. A new NIRSystems 6500 instrument was acquired during the course of this study. Data from the new instrument indicated that there were no longer differences between the different calibration sets, and therefore no longer differences between environments. Spectra for different samples were very close, the differences being of the same scale as those recorded with repeated measures of the same samples, or between the readings for the standard solvent solution. This led to the conclusion that the differences observed on the original NIRSystems 5000 instrument were due to instrument error, not environmental differences. More importantly, the different calibration sets were not comparable despite being similar to each other. Prediction from one calibration set to another was low. These observations led to the conclusion that NIRS was not a suitable method for determining chemical compounds associated with tolerance of sugarcane genotypes to eldana borer. The original NIRS instrument was subject to error, and the small number of calibration sets included in the study led to the erroneous conclusion that NIRS was suitable for the prediction of varietal tolerance to eldana. With the acquisition of the new instrument, the errors generated by the old instrument became apparent. With the increase in number of calibration sets included in the study, it also became apparent that a global calibration covering all environments was not possible. An analysis of the heritability of the chemical compounds associated with eldana resistance was also included in this study. A biparental progeny design of 24 crosses with 33 unselected offspring per cross was used. This trial would have been analysed once the calibration had been developed using the environmental trial, and it would have provided knowledge of the breeding behaviour of the chemical compounds associated with tolerance to eldana. Because the NIRS technique proved to be unsuitable for detection of chemical compounds associated with eldana resistance, the heritability of these chemical compounds could not be studied. As the NIRS study did not produce data, the G x E interaction analysis and determination of heritability was applied to the bud scale mass data set. This study showed a relatively low positive correlation between bud scale mass and resistance to eldana. The broad sense heritability estimate for bud scale mass from the G x E interaction analysis was 0.45, and the narrow sense heritability estimate from parent-offspring regression analysis was approximately 0.27, suggesting a low degree of genetic determination in bud scale mass. The G x E interaction analyses gave varying results depending on the method used. The ANOVA analysis suggested that ages, sites and years had an effect on bud scale mass, while deviation from maximum plot showed no significance for G x E interactions. The number and choice of genotypes selected as unstable also varied with the method used to determine the stability of individual genotypes. Regression analysis and rank order analysis revealed a number of unstable genotypes, whilst stability variance and ecovalence, which produced similar results, detected only two unstable genotypes. In the rank order analysis correction of data to remove genotype effect, reduced the number of unstable genotypes, suggesting that the G x E interaction effect was partially confounded with the bud scale mass of the genotypes. This was a more reliable method than the uncorrected rank order analysis, and would be the preferred analysis type of all those tried. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003.

The aim of the research presented in this dissertation was to further the implementation of push-pull for control of Eldana saccharina on sugarcane in the Midlands North region, KwaZulu-Natal, South Africa. Eldana saccharina, an indigenous stem borer, is the most damaging pest of sugarcane in South Africa, and sustainable control has still not been achieved. The push-pull strategy, a form of habitat management, has been developed for E. saccharina and is recommended as part of an integrated pest management (IPM) approach. Implementation of this strategy for both large- and small-scale farmers was facilitated through mixed methods social research. It included a novel exploratory network analysis to understand the process of technology adoption by farmers. Surveys showed that large-scale farmers have a good knowledge of E. saccharina, IPM and push-pull but that they needed more practical knowledge for implementation of the strategy. Farmers recommended experiential learning opportunities such as field days and model farms to get to know more about this technology. Despite demonstrating a positive attitude towards push-pull, farmers perceived it to be a ‘hassle’ and this is potentially the biggest barrier to its adoption. However, with suitable learning opportunities for farmers and good support for planting inputs, implementation of push-pull is likely to succeed. Sugarcane was shown to play an important role in the livelihoods and farming systems of small-scale growers. They did not perceive E. saccharina as a serious production constraint and had poor knowledge of the pest and its control. Extension for small-scale growers in this region should focus primarily on weed management and on reducing input costs, but still raising awareness of the increasing threat of E. saccharina. On-farm push-pull field trials showed a significant reduction of E. saccharina damage on two farms. Mean percentage damaged internodes decreased from 4.1% to 2.7% and from 1.7% to 1.1% in the presence of the repellent grass species, Melinis minutiflora. Where farmers did not manage their crops well, push-pull was not effective. It is therefore crucial that push-pull within an IPM framework be implemented together with good crop management practices. Stem borer surveys in wetlands on sugarcane farms revealed a high diversity of indigenous stem borers and parasitoids, including a stem borer species, Pirateolea piscator, which may pose a threat to crops in the future. These findings, together with a literature review on the significance of on-farm biodiversity and ecosystem services, demonstrated the value which wetlands have for pest management on sugarcane farms. Wetland health assessments were used to develop a tool for farmers to assess and utilise the wetlands on their farms for improved management of E. saccharina. This study highlights the importance of a farmer-participatory approach to implementation of knowledge-intensive farming practices such as push-pull. The importance of wetlands for providing pest regulatory services on sugarcane farms has shown that environmental sustainability needs to become a fundamental principle of farming and agricultural research. Participatory implementation of push-pull, as recommended in this dissertation, could act as a driving force for agroecology in the South African sugar industry and move sustainable farming practices off the pages of journals and manuals onto farmers’ fields. / Thesis (MSc (Zoology))--North-West University, Potchefstroom Campus, 2013

The aim of the research presented in this dissertation was to further the implementation of push-pull for control of Eldana saccharina on sugarcane in the Midlands North region, KwaZulu-Natal, South Africa. Eldana saccharina, an indigenous stem borer, is the most damaging pest of sugarcane in South Africa, and sustainable control has still not been achieved. The push-pull strategy, a form of habitat management, has been developed for E. saccharina and is recommended as part of an integrated pest management (IPM) approach. Implementation of this strategy for both large- and small-scale farmers was facilitated through mixed methods social research. It included a novel exploratory network analysis to understand the process of technology adoption by farmers. Surveys showed that large-scale farmers have a good knowledge of E. saccharina, IPM and push-pull but that they needed more practical knowledge for implementation of the strategy. Farmers recommended experiential learning opportunities such as field days and model farms to get to know more about this technology. Despite demonstrating a positive attitude towards push-pull, farmers perceived it to be a ‘hassle’ and this is potentially the biggest barrier to its adoption. However, with suitable learning opportunities for farmers and good support for planting inputs, implementation of push-pull is likely to succeed. Sugarcane was shown to play an important role in the livelihoods and farming systems of small-scale growers. They did not perceive E. saccharina as a serious production constraint and had poor knowledge of the pest and its control. Extension for small-scale growers in this region should focus primarily on weed management and on reducing input costs, but still raising awareness of the increasing threat of E. saccharina. On-farm push-pull field trials showed a significant reduction of E. saccharina damage on two farms. Mean percentage damaged internodes decreased from 4.1% to 2.7% and from 1.7% to 1.1% in the presence of the repellent grass species, Melinis minutiflora. Where farmers did not manage their crops well, push-pull was not effective. It is therefore crucial that push-pull within an IPM framework be implemented together with good crop management practices. Stem borer surveys in wetlands on sugarcane farms revealed a high diversity of indigenous stem borers and parasitoids, including a stem borer species, Pirateolea piscator, which may pose a threat to crops in the future. These findings, together with a literature review on the significance of on-farm biodiversity and ecosystem services, demonstrated the value which wetlands have for pest management on sugarcane farms. Wetland health assessments were used to develop a tool for farmers to assess and utilise the wetlands on their farms for improved management of E. saccharina. This study highlights the importance of a farmer-participatory approach to implementation of knowledge-intensive farming practices such as push-pull. The importance of wetlands for providing pest regulatory services on sugarcane farms has shown that environmental sustainability needs to become a fundamental principle of farming and agricultural research. Participatory implementation of push-pull, as recommended in this dissertation, could act as a driving force for agroecology in the South African sugar industry and move sustainable farming practices off the pages of journals and manuals onto farmers’ fields. / Thesis (MSc (Zoology))--North-West University, Potchefstroom Campus, 2013

Host recognition behaviour of two braconid larval parasitoids Cotesia sesamiae and Cotesia flavipes was studied using suitable stemborer hosts [i.e. Busseola fusca for C. sesamiae, and Chilo partellus for C. flavipes] and one non-host [Eldana saccharina]. The wasps displayed similar sequences of behavioural steps when locating their hosts largely depending on their antennae for host recognition and both antennae and tarsi for final host acceptance and oviposition. Tactile and contact chemoreception stimuli from the hosts seem to play a major role in oviposition decision by the parasitoids. In addition, the external morphology and distribution pattern of sensilla present on antennae, tarsi and ovipositor of the parasitoids were examined by scanning electron and optic microscopy after staining with silver nitrate. Three sensillar types were identified on the distal antennomeres: (i) non-porous sensilla trichoidea most probably involved in mechanoreception, (ii) uniporous sensilla chaetica likely to be gustatory and, (iii) multiporous sensilla placodea likely to be olfactory. The tarsi possess a few sensilla chaetica which could be gustatory while the manubrium is likely to be used in detection of vibrations. The distal end of the ovipositor bears numerous multiporous dome-shaped sensilla. Additionally, the ability of the wasps to discriminate between contact cues was studied. When host larvae were washed in distilled water the wasps did not insert their ovipositors. However, ovipositor insertion resumed when washed host or non-host larvae were painted with water extracts of their respective host larvae. The water extracts of the suitable hosts were more attractive to the wasps than those of non-hosts. Similarly, the frass is important in host recognition during short-range examination as those of respective hosts are more intensely antennated than of non-hosts. The parasitoids were able to discriminate the regurgitant of E. saccharina by not antennating the cotton wool ball of this host; while the regurgitant of B. fusca and C. partellus appeared not useful in discriminating between the two species for both parasitoid species. Further analysis suggests the presence of a protein(s) component(s) in the regurgitant possibly responsible for host recognition and oviposition by C. flavipes. / Thesis (Ph.D. (Environmental Science))--North-West University, Potchefstroom Campus, 2009.

Host recognition behaviour of two braconid larval parasitoids Cotesia sesamiae and Cotesia flavipes was studied using suitable stemborer hosts [i.e. Busseola fusca for C. sesamiae, and Chilo partellus for C. flavipes] and one non-host [Eldana saccharina]. The wasps displayed similar sequences of behavioural steps when locating their hosts largely depending on their antennae for host recognition and both antennae and tarsi for final host acceptance and oviposition. Tactile and contact chemoreception stimuli from the hosts seem to play a major role in oviposition decision by the parasitoids. In addition, the external morphology and distribution pattern of sensilla present on antennae, tarsi and ovipositor of the parasitoids were examined by scanning electron and optic microscopy after staining with silver nitrate. Three sensillar types were identified on the distal antennomeres: (i) non-porous sensilla trichoidea most probably involved in mechanoreception, (ii) uniporous sensilla chaetica likely to be gustatory and, (iii) multiporous sensilla placodea likely to be olfactory. The tarsi possess a few sensilla chaetica which could be gustatory while the manubrium is likely to be used in detection of vibrations. The distal end of the ovipositor bears numerous multiporous dome-shaped sensilla. Additionally, the ability of the wasps to discriminate between contact cues was studied. When host larvae were washed in distilled water the wasps did not insert their ovipositors. However, ovipositor insertion resumed when washed host or non-host larvae were painted with water extracts of their respective host larvae. The water extracts of the suitable hosts were more attractive to the wasps than those of non-hosts. Similarly, the frass is important in host recognition during short-range examination as those of respective hosts are more intensely antennated than of non-hosts. The parasitoids were able to discriminate the regurgitant of E. saccharina by not antennating the cotton wool ball of this host; while the regurgitant of B. fusca and C. partellus appeared not useful in discriminating between the two species for both parasitoid species. Further analysis suggests the presence of a protein(s) component(s) in the regurgitant possibly responsible for host recognition and oviposition by C. flavipes. / Thesis (Ph.D. (Environmental Science))--North-West University, Potchefstroom Campus, 2009.

Organic insecticides play a big role in reducing the usage of chemical insecticides and their negative impact on the environment. Bacillus thuringiensis (Bt) spays are the only tool that organic farmers are allowed to use for the control of pests. Genetic engineering and modification of crops have been made possible with scientific advances in cell and molecular biology. These advances are used to transfer some of the Bt Cry toxins into crops for control of target species to reduce yield loss. Bt maize were commercialised for the first time in South Africa in 1998 and the economic important stem borers, Busseola fusca (Fuller) (Lepidoptera: Noctuidae), Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) and Sesamia calamistis (Hampson) (Lepidoptera: Noctuidae) were exposed to the Cry1Ab toxin that is found in Bt maize. Busseola fusca developed resistance to Cry1Ab under field conditions within eight years after it had been released. Eldana saccharina (Walker) (Lepidoptera: Pyralidae) is a major pest on sugarcane in South Africa and although it has not been recorded on maize in this country, is it known as a major pest of maize in other African countries. African armyworm, Spodoptera exempta (Walker) (Lepidoptera: Noctuidae) has a very wide distribution in Africa and is known to be an occasional pest on maize. The aims of this dissertation were to determine the efficacy of Bt spray applications for control of four lepidopteran pests and whether development of Cry1Ab resistance by B. fusca caused a loss in susceptibility to other Bt toxins (i.e. cross-resistance). Susceptibility bioassays with 10 day old larvae were conducted under laboratory conditions. Treatments included application of various dosage rates of Dipel® and deltamethrin as well as exposure to MON810 (maize leaves). Stemborer populations of C. partellus, E. saccharina, and B. fusca (Venda) as well as the S. exempta were effectively controlled by the Bt spray, Dipel®. Care should be taken not be interpret the percentage C. partellus, E. saccharina and S. exempta larvae that survived after exposure to MON810 and Bt spray treatments as development of resistance without verification of these experiments with earlier instars that are known to be more susceptible. Spodoptera exempta is active throughout a year in temperate zones of Africa. If S. exempta develop resistance to Cry toxins and Bt maize events would be released for commercial planting in these areas, S. exempta pose a threat added to their injuriousness. Busseola fusca larvae were sampled from Venda (susceptible population), Ventersdorp and the Vaalharts Irrigation Scheme (resistant population). The Ventersdorp B. fusca population was controlled by MON810 and MON89034 and Bt sprays, but the percentage larvae that survived showed reduced susceptibility within the population. Dipel® treatments, MON810 and MON89034 did not provide effective control of the Vaalharts B. fusca population reported to be resistant to Cry1Ab, in two experiments. The high survival rates indicate a reduction in susceptibility to Cry toxins other than Cry1Ab and therefore development of cross resistance in the Vaalharts B. fusca population. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2014

Organic insecticides play a big role in reducing the usage of chemical insecticides and their negative impact on the environment. Bacillus thuringiensis (Bt) spays are the only tool that organic farmers are allowed to use for the control of pests. Genetic engineering and modification of crops have been made possible with scientific advances in cell and molecular biology. These advances are used to transfer some of the Bt Cry toxins into crops for control of target species to reduce yield loss. Bt maize were commercialised for the first time in South Africa in 1998 and the economic important stem borers, Busseola fusca (Fuller) (Lepidoptera: Noctuidae), Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) and Sesamia calamistis (Hampson) (Lepidoptera: Noctuidae) were exposed to the Cry1Ab toxin that is found in Bt maize. Busseola fusca developed resistance to Cry1Ab under field conditions within eight years after it had been released. Eldana saccharina (Walker) (Lepidoptera: Pyralidae) is a major pest on sugarcane in South Africa and although it has not been recorded on maize in this country, is it known as a major pest of maize in other African countries. African armyworm, Spodoptera exempta (Walker) (Lepidoptera: Noctuidae) has a very wide distribution in Africa and is known to be an occasional pest on maize. The aims of this dissertation were to determine the efficacy of Bt spray applications for control of four lepidopteran pests and whether development of Cry1Ab resistance by B. fusca caused a loss in susceptibility to other Bt toxins (i.e. cross-resistance). Susceptibility bioassays with 10 day old larvae were conducted under laboratory conditions. Treatments included application of various dosage rates of Dipel® and deltamethrin as well as exposure to MON810 (maize leaves). Stemborer populations of C. partellus, E. saccharina, and B. fusca (Venda) as well as the S. exempta were effectively controlled by the Bt spray, Dipel®. Care should be taken not be interpret the percentage C. partellus, E. saccharina and S. exempta larvae that survived after exposure to MON810 and Bt spray treatments as development of resistance without verification of these experiments with earlier instars that are known to be more susceptible. Spodoptera exempta is active throughout a year in temperate zones of Africa. If S. exempta develop resistance to Cry toxins and Bt maize events would be released for commercial planting in these areas, S. exempta pose a threat added to their injuriousness. Busseola fusca larvae were sampled from Venda (susceptible population), Ventersdorp and the Vaalharts Irrigation Scheme (resistant population). The Ventersdorp B. fusca population was controlled by MON810 and MON89034 and Bt sprays, but the percentage larvae that survived showed reduced susceptibility within the population. Dipel® treatments, MON810 and MON89034 did not provide effective control of the Vaalharts B. fusca population reported to be resistant to Cry1Ab, in two experiments. The high survival rates indicate a reduction in susceptibility to Cry toxins other than Cry1Ab and therefore development of cross resistance in the Vaalharts B. fusca population. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2014