Schrader, Jeffrey A.
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1990. Vita. Abstract. Includes bibliographical references (leaves 104-107). Also available via the Internet.
Buica, Astrid Sorina.
Thesis (Ph. D.)--University of Stellenbosch, 2007. Includes bibliographical references. Also available via the Internet.
Separation of proteins with capillary electrophoresis in coated capillaries with and without electroosmosis : studies on zone broadening and analytical performancesMohabbati, Sheila 2006 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. Härtill 4 uppsatser.
Diss. (sammanfattning) Linköping Univ. Härtill 7 uppsatser.
A rapid method for detecting single nucleotide polymorphisms using antimicrobial resistance in Neisseria gonorrhoeae as a modelCullingham, Kyle 26 April 2005 (has links)
Chromosomal mediated antimicrobial resistance in Neisseria gonorrhoeae can develop as a result of three main processes including the alteration of target enzymes, changes in transmembrane transport channels and active efflux pump function. Single nucleotide polymorphisms (SNPs) of target genes such as DNA gyrase (gyrA) and topoisomerase (parC), together with mutations in the promoter regions of the efflux pumps norM and mtr can confer resistance to the macrolides, penicillins and fluoroquinolones. These SNPs were analyzed using the SNaPshot method to allow for rapid detection of resistant isolates. Oligonucleotides were developed in the 5’ to the 3’ direction, ending one nucleotide adjacent to the specific SNP of interest. Single base extension reactions were performed and were detected using capillary electrophoresis. The SNaPshot procedure from Applied Biosystems employed in this study adds a single fluorescently-labelled nucleotide complementary to this SNP at the 3’ end by a primer extension polymerase reaction. Then using capillary electrophoresis, the labelled nucleotide is detected, enabling differentiation between A, C, T, or G. SNP results obtained were verified using DNA sequencing and both single and multiplexed reactions were carried out to increase the efficiency of the procedure. Spiked urine samples were also observed to determine if SNPs could be detected clinically. Single reactions enabled the characterization of all confirmed and relevant SNPs. With multiplex primer extension, multiple peaks were observed, each corresponding to one of the SNPs in the gene. This technique was explored for its applicability to detect SNPs of gyrA and parC mutations. Observable SNP detection limits were seen in spiked urine samples at 108 cells/mL in as early as 4 hours. DNA sequencing results confirmed the SNPs identity in each case. Thus, capillary electrophoresis using the SNaPshot protocol is another way to rapidly identify clinically resistant strains of Neisseria gonorrhoeae. This technique has also been shown to reduce analysis time compared to DNA sequencing and produces the same results. February 2005
Chan, Yick Chuen.
Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2002. Includes bibliographical references (leaves 78-80). Also available in electronic version. Access restricted to campus users.
Development and validation of analytical methodologies for the determination of ionic compounds in environmental, biological and industrial samples by capillary zone electrophoresisLau, Kap-man. 1997 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1998. Includes bibliographical references (leaves 402-404).
Beheshti, Afshin. Van Winkle, David H.
Thesis (Ph. D.)--Florida State University, 2002. Advisor: Dr. David H. Van Winkle, Florida State University, College of Arts and Sciences, Dept. of Physics. Title and description from dissertation home page (viewed Oct. 3, 2003). Includes bibliographical references.
An investigation on capillary electrophoresis-based immunoassay with laser induced fluorescence detectionOu, Jianping. 1999 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1999. Includes bibliographical references (leaves 122-135).
Shum, Kwok-yan, Daisy
Thesis (M. Phil.)--University of Hong Kong, 1978. Typescript.
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