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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Electrophoretic behaviour of polystyrene microspheres in agarose gels

杜光旭, To, Kwong-yuk. 1993
published_or_final_version Biochemistry Doctoral Doctor of Philosophy
22

Analysis of flavan-3-ols by capillary electrophoresis.

2004 (has links)
Lee Wai Hang. Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. Includes bibliographical references (leaves 71-76). Abstracts in English and Chinese. Acknowledgement --- p.i Abstract --- p.ii Table of Contents --- p.v Abbreviations --- p.viii List of Figures --- p.ix List of Tables --- p.xiv Chapter Chapter 1. --- Introduction --- p.1 Chapter 1.1 --- The French Paradox --- p.1 Chapter 1.2 --- Flavonoids --- p.2 Chapter 1.3 --- Grape seed extract --- p.4 Chapter 1.4 --- Instrunmental analysis --- p.7 Chapter 1.4.1 --- High Performance Liquid Chromatography --- p.7 Chapter 1.4.2 --- Colorimetry --- p.9 Chapter 1.5 --- Capillary Electrophoresis --- p.10 Chapter 1.5.1 --- Instrunmentation --- p.10 Chapter 1.5.2 --- Electroosmotic Flow --- p.11 Chapter 1.5.3 --- Electrophoretic mobility --- p.13 Chapter 1.6 --- Objective of the study --- p.15 Chapter 2. --- Experimental --- p.18 Chapter 2.1 --- Reagents and material --- p.18 Chapter 2.1.1 --- Reagents --- p.18 Chapter 2.1.2 --- Instrunmentation --- p.18 Chapter 2.1.3 --- Reference compounds --- p.19 Chapter 2.1.4 --- Samples --- p.19 Chapter 2.2 --- Selection of solvent for sample preparation --- p.20 Chapter 2.3 --- Procedures --- p.21 Chapter 2.3.1 --- Preparation of running buffer solution --- p.21 Chapter 2.3.2 --- Preparation of standard solution --- p.21 Chapter 2.3.3 --- Preparation of sample solution --- p.22 Chapter 2.3.4 --- Flushing procedures --- p.22 Chapter 3. --- Results and Discussion --- p.24 Chapter 3.1 --- Preliminary experiments --- p.24 Chapter 3.2 --- Effect of pH --- p.27 Chapter 3.3 --- Addition of surfactant --- p.30 Chapter 3.4 --- Effect of SDS concentration --- p.35 Chapter 3.5 --- Addition of cyclodextrins --- p.39 Chapter 3.6 --- Urea --- p.46 Chapter 3.7 --- Addition of organic modifier --- p.47 Chapter 3.8 --- Effect of borate concentration --- p.49 Chapter 3.9 --- Effect of cyclodextrin concentration --- p.53 Chapter 3.10 --- Optimized condition --- p.58 Chapter 3.11 --- Reproducibility of the method --- p.58 Chapter 3.12 --- Quantitative analysis of reference compounds --- p.60 Chapter 3.13 --- Application of the CE method in grape seed products --- p.62 Chapter 4. --- Conclusion --- p.69 References --- p.71
23

Determination of capillary electrophoretic methodology : its application as a mechanistic tool for gaining insight into the composition of, and detergent action on, coloured macromolecule fabric stains

Pretswell, Emma Louise 1995 (has links)
No description available.
24

Nuclear magnetic resonance studies of electrophoretic mobility in surfactant systems

Coveney, Francis Michael 1989 (has links)
No description available.
25

Microcolumn separations coupled to mass spectrometry : suitability for drug metabolism studies

Baynham, Michael Thomas 2000 (has links)
No description available.
26

Electrophoresis of Colloidal Particles in Shear-Thinning Polymer Solutions

Posluszny, Denise 1 August 2014 (has links)
This thesis includes a theoretical and experimental analysis of electrophoresis of colloidal particles in non-Newtonian polymeric fluids with shear-rate dependent viscosities. A model is derived that predicts field dependent electrophoretic mobility in shear-thinning Carreau uids. The latter effect is experimentally investigated for submicron particles in solutions of linear polyacrylamide using capillary electrophoresis. The mobilitiies of the particles studied in these solutions did not depend on field strength, yet the mobilities were consistently an order of magnitude greater than in water and glycerol solutions with similar bulk viscosities. The increase in particle mobility could be attributed to several mechanisms, however it is consistent with the depletion of polymer apparent viscosity of the fluid as it migrates by electrophoresis is the same as experienced by the particle in Brownian diffusion. A comparison of particle mobility in both glycerol and polyacrylamide solutions to diffusion coefficients of the particles measured by dynamic light scattering supports this conclusion.
27

Charging behaviour in a nonpolar colloidal system

Suparno. Unknown Date (has links)
Thesis (PhDAppliedPhysics)--University of South Australia, 2000.
28

Development of selective electrophoresis for proteins and peptides within proteomes

Ly, Linda, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW 2008 (has links)
Analysis of complex protein samples is demanding due to the wide dynamic range of expression levels and the limited detection range of technology. Proteomics relies heavily on the development of new fractionation strategies to help reduce complexity, and overcome the technological and biological challenges associated with proteome analysis. Here, the development of a prototype instrument named ??Microflow MF10?? was explored to enrich for particular classes of proteins. The MF10 was found to have a number of advantages over commercially available fractionation systems. Due to the reduced separation electrode distance, fractionation was rapid, occuring within ~0.125 kVH over 2-6 fractions under native conditions but longer under denaturing conditions. As low as 2 ng peptide could be fractionated with recovery for downstream analysis achievable. The ability to alter protein charge by changing the pH (acidic (pI 3.6) to basic environments (pI 10.4)) allows selection of proteins based on charge/mobility, size, shape, buffer ionic strength, pH and field strength. Proteins <10 kDa are also not routinely analysed because current technology is unable to cater for this region of the proteome. Peptide enrichment using the MF10 was achieved using a 7-protein/peptide standard mix (1-25 kDa), to the 1-5 kDa fraction with simultaneous fractionation of the higher mass protein standards. Plasma was also used to enrich for the peptidome (< 5 kDa) in the presence of the proteome. Enrichment of 73 proteins inclusive of 22 proteins in the 1-25 kDa fraction was achieved compared to a total equivalent of 42 proteins from unfractionated plasma. Rare samples (≤ 106 cells) from stem cell populations or derived clinically are challenging due to the absolute limits in protein copy number and abundance. CD34+ haematopoietic stem cells and CD4+/CD8+ T-cells were used to develop fractionation methods and elucidate the cell differentiation process. MF10 fractionation and analysis by SDS-PAGE and LC-MS/MS revealed 24 differentially expressed proteins between the 3 cell populations, which may be involved in cell differentiation. To quantify these expression differences, iTRAQ with 2-D LC-MS/MS was applied. This study has highlighted the challenges associated with samples of limited quantity. It has been successful in understanding the effects of various conditions on the electrophoretic mobility of proteins, which in proteomics, has remained largely unexplored.
29

Development of selective electrophoresis for proteins and peptides within proteomes

Ly, Linda, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW 2008 (has links)
Analysis of complex protein samples is demanding due to the wide dynamic range of expression levels and the limited detection range of technology. Proteomics relies heavily on the development of new fractionation strategies to help reduce complexity, and overcome the technological and biological challenges associated with proteome analysis. Here, the development of a prototype instrument named ??Microflow MF10?? was explored to enrich for particular classes of proteins. The MF10 was found to have a number of advantages over commercially available fractionation systems. Due to the reduced separation electrode distance, fractionation was rapid, occuring within ~0.125 kVH over 2-6 fractions under native conditions but longer under denaturing conditions. As low as 2 ng peptide could be fractionated with recovery for downstream analysis achievable. The ability to alter protein charge by changing the pH (acidic (pI 3.6) to basic environments (pI 10.4)) allows selection of proteins based on charge/mobility, size, shape, buffer ionic strength, pH and field strength. Proteins <10 kDa are also not routinely analysed because current technology is unable to cater for this region of the proteome. Peptide enrichment using the MF10 was achieved using a 7-protein/peptide standard mix (1-25 kDa), to the 1-5 kDa fraction with simultaneous fractionation of the higher mass protein standards. Plasma was also used to enrich for the peptidome (< 5 kDa) in the presence of the proteome. Enrichment of 73 proteins inclusive of 22 proteins in the 1-25 kDa fraction was achieved compared to a total equivalent of 42 proteins from unfractionated plasma. Rare samples (≤ 106 cells) from stem cell populations or derived clinically are challenging due to the absolute limits in protein copy number and abundance. CD34+ haematopoietic stem cells and CD4+/CD8+ T-cells were used to develop fractionation methods and elucidate the cell differentiation process. MF10 fractionation and analysis by SDS-PAGE and LC-MS/MS revealed 24 differentially expressed proteins between the 3 cell populations, which may be involved in cell differentiation. To quantify these expression differences, iTRAQ with 2-D LC-MS/MS was applied. This study has highlighted the challenges associated with samples of limited quantity. It has been successful in understanding the effects of various conditions on the electrophoretic mobility of proteins, which in proteomics, has remained largely unexplored.
30

Kinetic capillary electrophoresis and its applications

Berezovski, Maxim. 2005 (has links)
Thesis (Ph.D.)--York University, 2005. Graduate Programme in Chemistry. Typescript. Includes bibliographical references (leaves 140-150). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNR11550

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