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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
61

A rapid method for detecting single nucleotide polymorphisms using antimicrobial resistance in Neisseria gonorrhoeae as a model

Cullingham, Kyle 26 April 2005 (has links)
Chromosomal mediated antimicrobial resistance in Neisseria gonorrhoeae can develop as a result of three main processes including the alteration of target enzymes, changes in transmembrane transport channels and active efflux pump function. Single nucleotide polymorphisms (SNPs) of target genes such as DNA gyrase (gyrA) and topoisomerase (parC), together with mutations in the promoter regions of the efflux pumps norM and mtr can confer resistance to the macrolides, penicillins and fluoroquinolones. These SNPs were analyzed using the SNaPshot method to allow for rapid detection of resistant isolates. Oligonucleotides were developed in the 5’ to the 3’ direction, ending one nucleotide adjacent to the specific SNP of interest. Single base extension reactions were performed and were detected using capillary electrophoresis. The SNaPshot procedure from Applied Biosystems employed in this study adds a single fluorescently-labelled nucleotide complementary to this SNP at the 3’ end by a primer extension polymerase reaction. Then using capillary electrophoresis, the labelled nucleotide is detected, enabling differentiation between A, C, T, or G. SNP results obtained were verified using DNA sequencing and both single and multiplexed reactions were carried out to increase the efficiency of the procedure. Spiked urine samples were also observed to determine if SNPs could be detected clinically. Single reactions enabled the characterization of all confirmed and relevant SNPs. With multiplex primer extension, multiple peaks were observed, each corresponding to one of the SNPs in the gene. This technique was explored for its applicability to detect SNPs of gyrA and parC mutations. Observable SNP detection limits were seen in spiked urine samples at 108 cells/mL in as early as 4 hours. DNA sequencing results confirmed the SNPs identity in each case. Thus, capillary electrophoresis using the SNaPshot protocol is another way to rapidly identify clinically resistant strains of Neisseria gonorrhoeae. This technique has also been shown to reduce analysis time compared to DNA sequencing and produces the same results. February 2005
62

Microsecond electrophoresis

Plenert, Matthew Lee 2006 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2006. Vita. Includes bibliographical references.
63

Electrophoretisch onderzoek van het mechanisme van enige klinisch gebruikelijke serumvlokkingsreacties

Verschure, Johannes Christiaan Mari. 1946 (has links)
Academisch proefschrift - Utrecht. Summary in Dutch and English. Cover title: Electrophorese en serumvlokkingsreacties.
64

Phospholipid enhanced separation with capillary electrophoresis

Luo, Ruijuan. Unknown Date (has links)
Thesis (Ph. D.)--West Virginia University, 2010. Title from document title page. Document formatted into pages; contains xviii, 145 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.
65

Microparticle influenced electroosmotic flow

Young, John M. 2005 (has links) (PDF)
Thesis (M.S.)--Brigham Young University. Dept. of Mechanical Engineering, 2005. Includes bibliographical references (p. 91-94).
66

Membrane-based protein preconcentration microfluidic devices

Li, Yi 2006 (has links) (PDF)
Thesis (M.S.)--Brigham Young University. Dept. of Chemistry and Biochemistry, 2006. Includes bibliographical references
67

DNA electrophoresis in microsystems integrated with sub-micron pillar arrays

Chan, Yick Chuen. 2006 (has links)
Thesis (Ph.D.)--Hong Kong University of Science and Technology, 2006. Includes bibliographical references (leaves 132-138). Also available in electronic version.
68

Ultrasensitive one- and two- dimensional capillary electrophoresis for single cell analysis

Fazal, Md Abul. 2005 (has links)
Thesis (Ph. D.)--University of Washington, 2005. Includes bibliographical references (leaves 208-217).
69

Photo-initiated cross-linked polyacrylamide gels for microdevice electrophoresis

Agrawal, Shilpa 29 August 2005 (has links)
Photo-polymerized cross-linked polyacrylamide gels are becoming increasingly important for use in micro-fabricated DNA electrophoresis systems because they allow a concentrated sieving matrix to be precisely positioned at any location within a complex micro-channel network. The rate of photo-initiation in the free radical gel polymerization reaction, however, can exert a strong influence on the resulting gel structure. Experimental data on separation resolution of single stranded DNA (ssDNA) in photoinitiated polyacrylamide gels is very sparse. In this study, we investigate the performance of ssDNA electrophoresis in an ALF Express automated DNA sequencer using various photo-initiation chemistries. Cross-linked polyacrylamide gels with concentrations ranging from 6 to 12 %T were prepared using riboflavin, methylene blue, irgacure 651 and ReproGel (AP Biotech) photo-initiators. Separation resolution is compared with that attained in cross-linked polyacrylamide gels prepared using conventional chemical initiators (e.g. ammonium persulfate/ Tetramethylethylenediamine) in order to determine the polymerization conditions necessary for optimum performance.
70

Non-aqueous, capillary electrophoretic separations of enantiomers with a charged cyclodextrin highly-soluble in organic solvents

Sanchez Vindas, Silvia Elena 2004 (has links)
The synthesis of the sodium salt of heptakis (2, 3-di-O-acetyl-6-O-sulfo)-β-cyclodextrin was modified to increase the isomeric purity to 98.5%. This salt was used to obtain the organic-solvent-soluble, single-isomer, charged tetrabutylammonium salt of heptakis (2, 3-di-O-acetyl-6-O-sulfo)-β-cyclodextrin. Its isomeric purity was higher than 99%, as determined by CE, and its structure was confirmed by NMR and ESI-MS analysis. The hydrophobic single-isomer cyclodextrin was utilized to separate the enantiomers of weak base analytes in aprotic media by capillary electrophoresis. The effective mobilities and separation selectivities follow trends observed with negatively charged cyclodextrins in amphiprotic solvents. The properties of the dissolved cyclodextrin are altered by its counter ion, thereby affecting the separations of enantiomers. The aprotic media allow the modification of the separation selectivity, since the binding strength of the enantiomers to the cyclodextrin is intermediate between that reported in aqueous and methanolic buffers.

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